Comparison of transgenic plant production for bacterial blight resistance in Pakistani local rice (Oryza sativa L.) cultivars

The study was carried out to improve bacterial leaf blight resistance in three rice cultivars (Basmati - 370, DR - 82 and IR - 6) by Agrobacterium mediated transformation system. Three week-old scutellum derived calli were infected with Agrobacterium strain EHA101, containing binary vector pTCL5 which has Xa 21 gene. Different levels of acetosyringone were tested to enhance transformation efficiency. Acetosyringone at 300 ìM showed 56.6% GUS expression with 100 and 200 ìM acetosyringone showing 13.3 and 30.0% GUS expression, respectively. Maximum transformation efficiency was obtained using DR - 82 with calli exposed to 300 ìM acetosyringone for 2 min. Direct hygromycin selection with 48 h ofco-cultivation was superior to pre-selection in all three cultivars. Transient GUS expression was 51.4% while stable GUS expression in calli was 18.8%. PCR analysis confirmed the presence of the Xa 21 gene in transformed regenerated plants. Stable varietal transformation efficiency was DR - 82 > Basmati-370 > IR - 6. Resistance of transgenic plants against Xanthomonas oryzae pathovar oryzae was evaluated with various strains/isolates at the seedling stage. All PCR positive transgenic plants of DR - 82 and Basmati - 370 were resistant with lesion areas less than 5% of the inoculated leaf area. The tested transgenic plants were resistant to all the indigenous and exotic strains tested due to the broad spectrum protection provided by the Xa 21 gene

Stereo Vision-based Semantic 3D Object and Ego-motion Tracking for Autonomous Driving

We propose a stereo vision-based approach for tracking the camera ego-motion and 3D semantic objects in dynamic autonomous driving scenarios. Instead of directly regressing the 3D bounding box using end-to-end approaches, we propose to use the easy-to-labeled 2D detection and discrete viewpoint classification together with a light-weight semantic inference method to obtain rough 3D object measurements. Based on the object-aware-aided camera pose tracking which is robust in dynamic environments, in combination with our novel dynamic object bundle adjustment (BA) approach to fuse temporal sparse feature correspondences and the semantic 3D measurement model, we obtain 3D object pose, velocity and anchored dynamic point cloud estimation with instance accuracy and temporal consistency. The performance of our proposed method is demonstrated in diverse scenarios. Both the ego-motion estimation and object localization are compared with the state-of-of-the-art solutions.Comment: 14 pages, 9 figures, eccv201

Virtual Immortality: Reanimating Characters from TV Shows.

The objective of this work is to build virtual talking avatars of characters fully automatically from TV shows. From this unconstrained data, we show how to capture a character's style of speech, visual appearance and language in an e ort to construct an interactive avatar of the person and e ectively immortalize them in a computational model. We make three contributions (i) a complete framework for producing a generative model of the audiovisual and language of characters from TV shows; (ii) a novel method for aligning transcripts to video using the audio; and (iii) a fast audio segmentation system for silencing nonspoken audio from TV shows. Our framework is demonstrated using all 236 episodes from the TV series Friends [34] ( 97hrs of video) and shown to generate novel sentences as well as character specific speech and video

Degradability of cross-linked polyurethanes based on synthetic polyhydroxybutyrate and modified with polylactide

In many areas of application of conventional non-degradable cross-linked polyurethanes (PUR), there is a need for their degradation under the influence of specific environmental factors. It is practiced by incorporation of sensitive to degradation compounds (usually of natural origin) into the polyurethane structure, or by mixing them with polyurethanes. Cross-linked polyurethanes (with 10 and 30%wt amount of synthetic poly([R,S]-3-hydroxybutyrate) (R,S-PHB) in soft segments) and their physical blends with poly([d,l]-lactide) (PDLLA) were investigated and then degraded under hydrolytic (phosphate buffer solution) and oxidative (CoCl2/H2O2) conditions. The rate of degradation was monitored by changes of samples mass, morphology of surface and their thermal properties. Despite the small weight losses of samples, the changes of thermal properties of polymers and topography of their surface indicated that they were susceptible to gradual degradation under oxidative and hydrolytic conditions. Blends of PDLLA and polyurethane with 30 wt% of R,S-PHB in soft segments and PUR/PDLLA blends absorbed more water and degraded faster than polyurethane with low amount of R,S-PHB

The Rate of Recurrence of Club Foot Deformity in Patients Using Dennis Brown Splint

Objective: To estimate the rate of recurrence of deformity in patients using Dennis Brown Splint and for the description of the issues related to non-compliance of the Dennis Brown Splint. Material and Methods: It was a prospective descriptive case series study.70 patients with corrected Club Feet were selected from APPNA Rehabilitation Department of Benazir Bhutto Hospital. The patients were given the Dennis Brown Splints for the maintenance of the correction followed by Ponseti method. Clubfeet were graded according to the Pirani scoring system check the recurrence of the deformity. Patients were called for routine follow-up to check the rate of recurrence of the deformity and parents were interviewed for the issues related to non-compliance to the bracing program. Duration of the study was 12 months followed by the bracing. Results: It is founded that the recurrence of the deformity is about 24%. 17/70 reported with recurrence with the deformity after correction during bracing program. While the issues of non-compliance 47% reported with skin problems while 45% reported with residual adduction of the foot part due to loosening of the screw. 30 % reported that their babies withdraw the foot from the shoes by applying force. 70% of the families don’t have even basic education which also adds to the poor outcomes. Conclusion: It was concluded that relapse rate is 24% because of the issues related to the non-compliance from which main issue is skin damage. The second other cause is repeated residual adduction due to the loosening of the screw. Most of the families have very low education so it was founded very difficult to counsel them. Some families also quit from bracing without informing the consultants

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