A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Distribution of races and Tox genes in Pyrenophora tritici-repentis isolates from wheat in Argentina

Tan spot, caused by Pyrenophora tritici-repentis, is a common disease in wheat-growing regions of Argentina. In this study 65 isolates of P. tritici-repentis obtained from different cultivars and wheat regions of Argentina were assessed for their virulence on six wheat cultivars/lines (Glenlea, Salomouni, Katepwa, M-3, 6B365 and 6B662) and for the presence/absence of the Tox genes based on a PCR approach. Thirty-six isolates were assigned to races, of which races 4 and 8 were dominant. Results for molecular analysis of ToxA, ToxB, ToxB-like and toxb genes showed that 57 isolates possessed the ToxA gene whereas only one isolate possessed ToxA and ToxB genes. There was no correlation between races and the toxin genotypes. It is suggested that P. tritici-repentis exhibits a complex race structure in Argentina

Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines

We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was obtained from an oocyte classified as failed to fertilise and subsequently chemically activated. We report the characterisation of pluripotency and the differentiation potential of these lines. Work is in progress to establish novel methods of stem cell derivation and culture, which will avoid the use of xenobiotics and be relevant to clinical production of human embryonic stem cell lines. Both newly derived human embryonic stem cell lines will be available for the research community from the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk)