PENGARUH SALEP EKSTRAK ETANOL DAUN SIRIH (Piper betle)</i> TERHADAP PENYEMBUHAN LUKA IRIS PADA TIKUS PUTIH JANTAN (Rattus norvegicus)

ABSTRAK Latar Belakang Tanaman obat yang banyak digunakan oleh masyarakat untuk mengobati luka adalah daun sirih (Piper betle). Daun sirih juga banyak dimanfaatkan untuk mengobati sariawan. Tujuan Penelitian ini bertujuan untuk menguji pengaruh salep ekstrak etanol daun sirih terhadap penyembuhan luka iris pada tikus putih jantan (Rattus norvegicus). Metode Penelitian ini menggunakan rancang penelitian eksperimental. Subjek penelitian dikelompokkan secara acak menjadi 3 kelompok. Masing-masing kelompok terdiri dari 10 ekor tikus, kemudian dilukai sepanjang 2 cm kedalaman 0,5 cm dan diberi perlakuan yang berbeda. Kelompok I merupakan kelompok perlakuan dengan pemberian salep ekstrak etanol daun sirih. Kelompok II merupakan kontrol positif dengan pemberian povidon iodine 10%, dan kelompok III adalah kontrol negatif dengan pemberian vaselin album. Panjang luka masing-masing kelompok diukur dan diamati setiap hari hingga hari ke-14, kemudian data yang didapatkan dianalisis menggunakan Oneway Anova. Hasil Lama penyembuhan luka iris kelompok uji dengan salep ekstrak etanol daun sirih, kelompok uji dengan povidon iodin dan kelompok uji dengan vaselin album berturut turut adalah 10,8±0,422; 11,9±0,316; 13,8±0,422 (p=0,000). Kesimpulan Penelitian pada tikus putih jantan (Rattus norvegicus) menunjukkan bahwa salep ekstrak etanol daun sirih mampu mempercepat proses penyembuhan luka. Salep daun sirih dapat dimanfaatkan dan menjadi alternatif terapi baru untuk penyambuhan luka. Kata Kunci : Salep daun sirih (Piper betle), Penyembuhan luka iris.   ABSTRACT Background One of the medicinal plants used to treat wounds is betel leaf. Betel leaf (Piper betle) is also used by Indonesian since the first medication to treat ulcers. However, there is currently no scientific evidence of the effects of betel leaf to accelerate incision wound healing in experimental animals or in humans. Objective The study aims to assess the ethanol extract of Piper betle leaf ointment on the healing of incised wounds in the rats Methods This type of study was purely experimental design. Research subjects were randomly divided into three groups and each group consisted of 10 rats. The incision wound were made 2-cm long and 0.5-cm deep and were given different treatments. Group I was treated with the ointment of betel leaf extract. Group II was a positive control by administering povidone-iodine 10%, and group III was a negative control with the provision of Vaseline-album. The length of incision wound in each group were measured and observed every day until the 14th day, then the data obtained were analyzed using Oneway Anova. Results Wound healing at the ethanol extract of betel leaf group, the povidone-iodine group and the vaseline-album groupwere 10,8±0,422; 11,9±0,316; 13,8±0,422, respectively (p=0,000). Conclusion This study showed that the ointment of betel leaf had the shortest healing times. Later, Ointment betel leaf can be utilized and become a new therapeutic alternative for wound healing. Keywords : Piper betle leaf ointment, incision wound  

Pengaruh Frekuensi Menguras Terhadap Jumlah Candida sp. Pada Air Bak Toilet Wanita di SPBU Surakarta

Candida sp. are dimorphic fungi that grow as the yeast cells. These fungi are commonly found on stagnant water, for example is toilet water tub. Several factors that affect the growth of Candida sp. on the toilet water tub are the source of water, toilet users, and the draining. The purpose of this research is to find out the effect of draining frequency on the number of Candida sp. in women’s toilet water tub at the gas station in Surakarta. This study is an observational analytical research with cross sectional approach. The examination was showed that the average number of Candida sp. in the regularly drained toilet water tubs was 66,67 colonies/10ml sample and the average number ofCandida sp. in the irregularly drained toilet water tubs was 240,3 colonies/10ml sample. The datawere tested by using independent t-tes and the significant number was 0,003 (P < 0,05). It could beconcluded that there was a distinctive effect of draining frequency on the number of Candida sp. inwomen’s toilet water tub at the gas station in Surakarta.Keywords: Candida sp., draining, water tu

ANTIFUNGAL AND ANTIBACTERIAL ACTIVITIES OF JUICE AND ETHANOLIC EXTRACTS OF GARCINIA MANGOSTANA L. LEAVES

Objective: Garcinia mangostana is a plant that can be used as a traditional medicine to treat various infectious diseases for the treatment of diarrhea, skin infection, and chronic wounds. The activity as antifungal and antibacterial of juice and ethanolic extract from G. mangostana leaves were investigated. Methods: Juice and ethanolic extract were concentrated using a rotary evaporator to get concentrated extract with rendemen 2.571 and 5.647% (w/w). Juice and ethanolic extract dilution method were employed to evaluate the antifungal activity against Saccharomyces cerevisiae. Ethanolic extract dilution method was used to assess the antibacterial activity against Bacillus subtilis and Escherichia coli. Results: The results of this research showed that juice and ethanolic extract were effective against S. cerevisiae, and the minimum inhibitory concentration was 1000, and 500 mg/mL. Antibacterial activity of the G. mangostana leaves ethanolic extract showed that the action was potential with the inhibition zone in B. subtilis and E. coli. Conclusions: The conclusion of this study is that juice and ethanolic extract of G. mangostana leaves have possible antifungal and antibacterial activity

POTENSI PENGEMBANGAN GRAFIT UNTUK PROSES ELEKTRIFIKASI SEBAGAI BAHAN BAKU BATERAI GUNA MENDUKUNG INDUSTRI PERTAHANAN STRATEGIS

Pengembangan teknologi elektrifikasi dalam industri pertahanan strategis memerlukan bahan baku yang efisien dan dapat diandalkan untuk memenuhi kebutuhan baterai. Grafit, merupakan bahan yang terdapat di Indonesia sebagai salah satu material yang memiliki sifat konduktif dan kapasitas penyimpanan energi yang baik, memiliki potensi yang besar dalam mendukung proses elektrifikasi ini. Pengembangan bahan baku grafit ini bertujuan untuk mengetahui potensi pengembangan grafik sebagai bahan baku baterai guna mendukung industri pertahanan strategis. Metode penelitian yang digunakan meliputi studi literatur yang melibatkan penelusuran sumber daya ilmiah terkini yang relevan dengan topik ini. Hasil studi menunjukkan bahwa grafit memiliki karakteristik yang sangat diinginkan untuk aplikasi baterai dalam industri pertahanan strategis. Grafit memiliki konduktivitas listrik yang baik dan mampu menyimpan energi dalam jumlah yang signifikan. Selain itu, grafit juga memiliki stabilitas termal yang tinggi dan ketahanan terhadap kerusakan fisik, sehingga menjadikannya sebagai kandidat yang menjanjikan dalam pengembangan baterai. Namun, terdapat beberapa tantangan dalam pengembangan grafit sebagai bahan baku baterai. Salah satunya adalah memperbaiki kapasitas penyimpanan energi yang terbatas pada grafit saat ini. Penelitian lebih lanjut diperlukan untuk mengatasi tantangan ini dan memaksimalkan potensi penggunaan grafit dalam industri pertahanan strategis. Pengembangan grafit sebagai bahan baku baterai memiliki potensi yang signifikan dalam mendukung proses elektrifikasi dalam industri pertahanan strategis. Namun, tantangan teknis perlu diatasi melalui penelitian dan pengembangan lebih lanjut. Dengan memperluas pengetahuan dan penerapan teknologi grafit, diharapkan dapat mendorong kemajuan dalam industri pertahanan strategis dan menghadirkan solusi yang inovatif untuk kebutuhan energi yang berkelanjutan

Effect of feed supplement modifications using keratinolytic bacteria on growth performance and meat chemical composition of domesticated quail

This study was carried out to determine a novel strategy for utilization of feather in poultry feed, along with the addition of immobilized keratinolytic Bacillus sp. SLII–1 as a supplement. Eighty male quails (day–old) were randomly divided into four diet groups, with each group receiving four different treatments. The results showed that Bacillus sp. SLII–I exhibited keratinolytic potential by increasing the protein solubility of feather meal in a liquid medium. The immobilization of Bacillus sp. SLII–I using 3% sodium alginate and 0.8% chitosan composition occurred at approximately 4.05×107 CFU/g, while the viable cells were maintained in acidic medium (pH 3.0) at around 3.95×107 CFU/g. Quails aged 19–33 days, fed with feather meal and Bacillus sp. SLII–1, demonstrated significantly higher weight gain and lower feed conversion ratio (FCR) as compared to those treated with feather meal without supplements. There were no significant differences in the values of dry matter, ash, and crude protein content of the meat among the quails in all the treatments. Therefore, feed supplemented with immobilized bacteria and feather waste can be utilized as an alternative diet for quails. This way, the use of feed materials can be reduced, and at the same time, chicken feather waste can be effectively utilized

Immobilization of Bacillus sp. SLII-1 on Chitosan-Alginate Hybrid Material for Promising Feedstock Supplement

The increase in poultry meat production led to an increase in feed demand. On the other hand, poultry industry produces feather waste which composes 5-7 wt% of the total body weight. Feed diversification by utilizing feather waste can be an alternative to overcome the problem of the high price of feed and to reduce environmental waste. The use of feather as a feed has a disadvantage because of the low digestibility of feather waste. The strategy to increase the digestibility of feather waste is to deliver keratinolytic bacteria to poultry intestine using immobilization technique. The purpose of this study was to determine the sodium alginate and chitosan concentration of immobilant with the highest number of released cell in poultry digestive system simulation. Bacillus sp. SLII-I as keratinolytic bacteria was able to increase soluble protein in feather meal liquid medium by 117.69% (wt/v). The composition of sodium alginate (2% w/v) and chitosan (0,8% w/v) was the most effective in delivering bacteria to poultry intestine as much as 2.54x107 CFU per gram bead in digestive system simulation

Temporal variation of water suitability for paddy irrigation needs at karst springs influenced by allogenic recharge in Gunungsewu Karst Area, Indonesia

Irrigation, a critical element in farming, can fulfill crop water needs and increase agricultural productivity during the dry season, provided that the two necessary factors are met, namely water supply and water quality. Water quality is a principal factor in assessing whether or not a water body is usable as a source of irrigation. Excess or lack of elements in irrigation water may affect irrigated crops and soil. For maximum harvests, studies scrutinizing the suitability of water supply for irrigation become necessary. Beton Spring has a large discharge, which the people of Ponjong District, Gunungkidul Regency, rely on for their irrigation and fish farming practices. Uniquely, this karst spring receives allogenic recharges from outside the karst area. This research was intended to assess the suitability of water quality of Beton Spring for temporal irrigation purposes by Sodium analysis (Na%), Sodium Adsorption Ratio (SAR), USSL, and Wilcox. The elements observed in the analysis were Ca2+, Na+, Mg2+, K+ and electrical conductivity. Based on the overall analysis results of the water quality series—Sodium analysis (the water samples were classified as Excellent), SAR (Excellent), USSL (moderate to good), and Wilcox (Excellent to Good), Beton Spring produces good-quality water that is suitable for irrigation

Effect of Leadership Style Toward Indonesian Education Performance in Education 4.0 Era: A Schematic Literature Review

The purpose of writing this article is to conduct a systematic literature review, on 6 (six) articles of leadership models from reputable international journals. The method used is a systematic literature review of 6 articles published in reputable international journals that discuss leadership models in secondary and higher education institutions in education 4.0 era. The results of the systematic literature review show that the leadership models of the 6 articles show similar similarities, namely transactional, transformational, charismatic, bureaucratic, and democratic leadership that have a positive and significant effect on the performance of educational institutions