Indoor Air Quality in Elderly Centers: Pollutants Emission and Health Effects

The world population is ageing, in particular in the developed world, with a significant increase in the percentage of people above 60 years old. They represent a segment of the population that is more vulnerable to adverse environmental conditions. Among them, indoor air quality is one of the most relevant, as elders spend comparatively more time indoors than younger generations. Furthermore, the recent COVID-19 pandemic contributed immensely to raising awareness of the importance of breathing air quality for human health and of the fact that indoor air is a vector for airborne infections and poisoning. Hence, this work reviews the state of the art regarding indoor air quality in elderly centers, considering the type of pollutants involved, their emission sources, and their health effects. Moreover, the influence of ventilation on air quality is also addressed. Notwithstanding the potential health problems with the corresponding costs and morbidity effects, only a few studies have considered explicitly indoor air quality and its impacts on elderly health. More studies are, therefore, necessary to objectively identify what are the impacts on the health of elderly people due to the quality of indoor air and how it can be improved, either by reducing the pollutants emission sources or by more adequate ventilation and thermal comfort strategies. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Funding: This work was financially supported by base funding of the following projects: LA/P/0045/2020 (ALiCE), UIDB/00511/2020 (LEPABE), and UIDB/50022/2020 (LAETA), funded by national funds through FCT/MCTES (PIDDAC). Fátima Felgueiras gratefully acknowledges the Portuguese National Funding Agency for Science, Research, and Technology (FCT) for the financial support through the Grant BD/6521/2020. António Martins thanks FCT for funding through program DL 57/2016—Norma transitória. Teresa Mata gratefully acknowledge the funding of Project NORTE-06-3559-FSE-000107, cofinanced by Programa Operacional Regional do Norte (NORTE2020), through Fundo Social Europeu (FSE)

Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF

We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow we used the ‘Isotope-Coded Protein Labeling’ (ICPL) method for accurate relative quantitation of protein expression. Such workflow was successfully applied to a comparative proteome analysis of schizophrenia versus healthy control brain tissues and can be an alternative to proteome researches

The diagnostic value of symptoms of possible oesophagogastric cancers: a systematic review and meta-analysis

Abstracts of the Cancer and Primary Care Research International (Ca-PRI) Network Seventh Annual Meeting: New Partnerships in Primary Care Cancer Research. June 10-13, 2014, Winnipeg, Manitoba, CanadaOral presentation abstractThis is the author accepted manuscript. The final version is available from Wiley via the DOI in this record

COVID-19 and the targets of SDG 8: reflections on Brazilian scenario

Purpose: This paper aims to present reflections on the COVID-19 pandemic impacts in the search for the targets of Sustainable Development Goal 8 (SDG 8) disseminated by the United Nations. These reflections are made considering the Brazilian scenario. Design/methodology/approach: As this paper is a viewpoint, several information presented here are based on authors’ opinion. However, to enhance the information basis and present examples, a bibliographic research was also conducted. Findings: Analyzing the targets of SDG 8 and the probable crisis in the Brazilian economy as a consequence of the pandemic, the authors believe that this crisis will impact the achievement of these targets. Practical implications: The reflections presented here can contribute with the debates about the search for decent work in Brazil. It is worth to remember that the development of actions aligned with SDG 8 targets generates more than only economic gains, as these contribute for building a more just, egalitarian and inclusive society. Originality/value: This viewpoint calls attention to issues that demand urgent public policies; otherwise, these issues can generate losses to an entire generation of Brazilians

Determining emotional profile based on microblogging analysis

First Online 30 August 2019In general, groups of people are formed because of the similarities and affinities that members have with each other. Musical preferences, soccer teams or even similar behaviours are examples of similarities and affinities that motivate group formation. In social media, identifying these affinities is a difficult task because personal information is not easily identified. In this paper we present an alternative to identifying similarities between authors and their most frequent audience in Twitter, using emotional and grammatical writing style analysis. Through this study it is possible to define the creation of an emotional profile entirely based on the interactions of people, thus allowing software like chatbots to “learn emotions” and provide emotionally acceptable responses.This work has been supported by FCT – Fundação para a Ciéncia e Tecnologia within the Project Scope: UID/CEC/00319/2019

Behavioural syndrome in a solitary predator is independent of body size and growth rate.

Models explaining behavioural syndromes often focus on state-dependency, linking behavioural variation to individual differences in other phenotypic features. Empirical studies are, however, rare. Here, we tested for a size and growth-dependent stable behavioural syndrome in the juvenile-stages of a solitary apex predator (pike, Esox lucius), shown as repeatable foraging behaviour across risk. Pike swimming activity, latency to prey attack, number of successful and unsuccessful prey attacks was measured during the presence/absence of visual contact with a competitor or predator. Foraging behaviour across risks was considered an appropriate indicator of boldness in this solitary predator where a trade-off between foraging behaviour and threat avoidance has been reported. Support was found for a behavioural syndrome, where the rank order differences in the foraging behaviour between individuals were maintained across time and risk situation. However, individual behaviour was independent of body size and growth in conditions of high food availability, showing no evidence to support the state-dependent personality hypothesis. The importance of a combination of spatial and temporal environmental variation for generating growth differences is highlighted

Insulin modulates cytokine release and selectin expression in the early phase of allergic airway inflammation in diabetic rats

<p>Abstract</p> <p>Background</p> <p>Clinical and experimental data suggest that the inflammatory response is impaired in diabetics and can be modulated by insulin. The present study was undertaken to investigate the role of insulin on the early phase of allergic airway inflammation.</p> <p>Methods</p> <p>Diabetic male Wistar rats (alloxan, 42 mg/Kg, i.v., 10 days) and controls were sensitized by s.c. injection of ovalbumin (OA) in aluminium hydroxide 14 days before OA (1 mg/0.4 mL) or saline intratracheal challenge. The following analyses were performed 6 hours thereafter: a) quantification of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and cytokine-induced neutrophil chemoattractant (CINC)-1 in the bronchoalveolar lavage fluid (BALF) by Enzyme-Linked Immunosorbent Assay, b) expression of E- and P- selectins on lung vessels by immunohistochemistry, and c) inflammatory cell infiltration into the airways and lung parenchyma. NPH insulin (4 IU, s.c.) was given i.v. 2 hours before antigen challenge.</p> <p>Results</p> <p>Diabetic rats exhibited significant reduction in the BALF concentrations of IL-1β (30%) and TNF-α (45%), and in the lung expression of P-selectin (30%) compared to non-diabetic animals. This was accompanied by reduced number of neutrophils into the airways and around bronchi and blood vessels. There were no differences in the CINC-1 levels in BALF, and E-selectin expression. Treatment of diabetic rats with NPH insulin, 2 hours before antigen challenge, restored the reduced levels of IL-1β, TNF-α and P-selectin, and neutrophil migration.</p> <p>Conclusion</p> <p>Data presented suggest that insulin modulates the production/release of TNF-α and IL-1β, the expression of P- and E-selectin, and the associated neutrophil migration into the lungs during the early phase of the allergic inflammatory reaction.</p

Modifiers of (CAG)(n) instability in Machado-Joseph disease (MJD/SCA3) transmissions: an association study with DNA replication, repair and recombination genes.

Twelve neurological disorders are caused by gene-specific CAG/CTG repeat expansions that are highly unstable upon transmission to offspring. This intergenerational repeat instability is clinically relevant since disease onset, progression and severity are associated with repeat size. Studies of model organisms revealed the involvement of some DNA replication and repair genes in the process of repeat instability, however, little is known about their role in patients. Here, we used an association study to search for genetic modifiers of (CAG)n instability in 137 parent-child transmissions in Machado-Joseph disease (MJD/SCA3). With the hypothesis that variants in genes involved in DNA replication, repair or recombination might alter the MJD CAG instability patterns, we screened 768 SNPs from 93 of these genes. We found a variant in ERCC6 (rs2228528) associated with an expansion bias of MJD alleles. When using a gene-gene interaction model, the allele combination G-A (rs4140804-rs2972388) of RPA3-CDK7 is also associated with MJD instability in a direction-dependent manner. Interestingly, the transcription-coupled repair factor ERCC6 (aka CSB), the single-strand binding protein RPA, and the CDK7 kinase part of the TFIIH transcription repair complex, have all been linked to transcription-coupled repair. This is the first study performed in patient samples to implicate specific modifiers of CAG instability in humans. In summary, we found variants in three transcription-coupled repair genes associated with the MJD mutation that points to distinct mechanisms of (CAG)n instability.This work was supported by the Canadian Institutes of Health Research; and the Levesque Chair for research in Neurogenetics [to G. A. R.]; and the Portuguese Foundation for Science and Technology [SFRH/BPD/77969/2011 to S.M]

Towards the SDGs for gender equality and decent work: investigating major challenges faced by Brazilian women in STEM careers with international experience

This paper aims to understand the main difficulties faced by women throughout their careers in Brazil and abroad. Based on the information gathered from these experiences, it seeks to advance the discussion on women's participation in STEM focusing on SDG 5 (gender equality) and SDG 8 (decent work). The main difficulties experienced by women in STEM as discussed in the academic literature were mapped. This provided input to develop a questionnaire containing qualitative and quantitative questions used to conduct interviews with women working in STEM. The sample consisted of highly qualified professionals working in high positions in the hierarchies of multinational companies in the STEM field with experience both in Brazil and abroad. The data collected was analyzed using a mixed-methods approach, including content analysis for qualitative questions and the Grey Relational Analysis for quantitative questions. The results revealed that the lack of flexible work systems, the scarcity of gender-sensitive organizational policies and labor policies, and the prevalence of traditional cultural models are some of the main difficulties faced both in Brazil and abroad by the women interviewed. The need to discuss issues of gender equality and decent work in the early stages of education is important for increasing women’s participation in STEM, which is a critical factor in the development of inclusive organizations and in fully achieving the sustainable development of society. This paper presents a unique perspective of the perceived difficulties faced by executive women who worked in Brazil and in different countries (i.e., Canada, Denmark, France, Germany, Switzerland and the United States). Gender equality in organizations is highly context-dependent, and cross-cultural analysis generates relevant insights to face the challenges and advance the discussion on women’s participation in STEM

Experimental and theoretical investigation of ligand effects on the synthesis of ZnO nanoparticles

ZnO nanoparticles with highly controllable particle sizes(less than 10 nm) were synthesized using organic capping ligands in Zn(Ac)2 ethanolic solution. The molecular structure of the ligands was found to have significant influence on the particle size. The multi-functional molecule tris(hydroxymethyl)-aminomethane (THMA) favoured smaller particle distributions compared with ligands possessing long hydrocarbon chains that are more frequently employed. The adsorption of capping ligands on ZnnOn crystal nuclei (where n = 4 or 18 molecular clusters of(0001) ZnO surfaces) was modelled by ab initio methods at the density functional theory (DFT) level. For the molecules examined, chemisorption proceeded via the formation of Zn...O, Zn...N, or Zn...S chemical bonds between the ligands and active Zn2+ sites on ZnO surfaces. The DFT results indicated that THMA binds more strongly to the ZnO surface than other ligands, suggesting that this molecule is very effective at stabilizing ZnO nanoparticle surfaces. This study, therefore, provides new insight into the correlation between the molecular structure of capping ligands and the morphology of metal oxide nanostructures formed in their presence