A very long baseline interferometry sky survey

A systematic very long baseline interferometry (VLBI) sky survey, undertaken to find a suitable set of compact celestial radio sources from which a more complete VLBI reference frame can be constructed, discussed. The survey was conducted by searching known celestial radio sources for compact components by means of VLBI observations. Baseline lengths were about 7 x 10 to the 7th power RF wavelengths (lambda = 13.1 cm), so the spatial wavelengths being sampled by the interferometer were generally on the order of a few milliarcseconds. Hence, the radio sources detected have a measurable portion of their total flux density contained in components that are no more than a few milliarcseconds in angular extent. Existing information of radio sources were used as clues to source size

Modeling, assessment, and design of porous cells based on schwartz primitive surface for bone scaffolds

The design of bone scafolds for tissue regeneration is a topic of great interest, which involves diferent issues related to geometry of architectures, mechanical behavior, and biological requirements, whose optimal combination determines the success of an implant. Additive manufacturing (AM) has widened the capability to produce structures with complex geometries, which should potentially satisfy the diferent requirements. These architectures can be obtained by means of refned methods and have to be assessed in terms of geometrical and mechanical properties. In this paper a triply periodic minimal surface (TPMS), the Schwarz's Primitive surface (P-surface), has been considered as scafold unit cell and conveniently parameterized in order to investigate the efect of modulation of analytical parameters on the P-cell geometry and on its properties. Several are the cell properties, which can afect the scafold performance. Due to the important biofunctional role that the surface curvature plays in mechanisms of cellular proliferation and diferentiation, in this paper, in addition to properties considering the cell geometry in its whole (such as volume fraction or pore size), new properties were proposed. Tese properties involve, particularly, the evaluation of local geometrical-diferential properties of the P-surface. Te results of this P-cell comprehensive characterization are very useful for the design of customized bone scafolds able to satisfy both biological and mechanical requirements. A numerical structural evaluation, by means of fnite element method (FEM), was performed in order to assess the stifness of solid P-cells as a function of the changes of the analytical parameters of outer surface and the thickness of cell. Finally, the relationship between stifness and porosity has been analyzed, given the relevance that this property has for bone scafolds design

Altered Kv2.1 functioning promotes increased excitability in hippocampal neurons of an Alzheimer's disease mouse model.

Altered neuronal excitability is emerging as an important feature in Alzheimer's disease (AD). Kv2.1 potassium channels are important modulators of neuronal excitability and synaptic activity. We investigated Kv2.1 currents and its relation to the intrinsic synaptic activity of hippocampal neurons from 3xTg-AD (triple transgenic mouse model of Alzheimer's disease) mice, a widely employed preclinical AD model. Synaptic activity was also investigated by analyzing spontaneous [Ca(2+)]i spikes. Compared with wild-type (Non-Tg (non-transgenic mouse model)) cultures, 3xTg-AD neurons showed enhanced spike frequency and decreased intensity. Compared with Non-Tg cultures, 3xTg-AD hippocampal neurons revealed reduced Kv2.1-dependent Ik current densities as well as normalized conductances. 3xTg-AD cultures also exhibited an overall decrease in the number of functional Kv2.1 channels. Immunofluorescence assay revealed an increase in Kv2.1 channel oligomerization, a condition associated with blockade of channel function. In Non-Tg neurons, pharmacological blockade of Kv2.1 channels reproduced the altered pattern found in the 3xTg-AD cultures. Moreover, compared with untreated sister cultures, pharmacological inhibition of Kv2.1 in 3xTg-AD neurons did not produce any significant modification in Ik current densities. Reactive oxygen species (ROS) promote Kv2.1 oligomerization, thereby acting as negative modulator of the channel activity. Glutamate receptor activation produced higher ROS levels in hippocampal 3xTg-AD cultures compared with Non-Tg neurons. Antioxidant treatment with N-Acetyl-Cysteine was found to rescue Kv2.1-dependent currents and decreased spontaneous hyperexcitability in 3xTg-AD neurons. Analogous results regarding spontaneous synaptic activity were observed in neuronal cultures treated with the antioxidant 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). Our study indicates that AD-related mutations may promote enhanced ROS generation, oxidative-dependent oligomerization, and loss of function of Kv2.1 channels. These processes can be part on the increased neuronal excitability of these neurons. These steps may set a deleterious vicious circle that eventually helps to promote excitotoxic damage found in the AD brain

Results of the Australian geodetic VLBI experiment

The 250-2500 km baseline vectors between radio telescopes located at Tidbinbilla (DSS43) near Canberra, Parkes, Fleurs (X3) near Sydney, Hobart and Alice Springs were determined from radio interferometric observations of extragalactic sources. The observations were made during two 24-hour sessions on 26 April and 3 May 1982, and one 12-hour night-time session on 28 April 1982. The 275 km Tidbinbilla - Parkes baseline was measured with an accuracy of plus or minus 6 cm. The remaining baselines were measured with accuracies ranging from 15 cm to 6 m. The higher accuracies were achieved for the better instrumented sites of Tidbinbilla, Parkes and Fleurs. The data reduction technique and results of the experiment are discussed

Optimal design of drip irrigation submains: presure-compensating emitters

For a drip irrigation system to be successful, it must he well designed, properly installed, managed and maintained. In plots with steep slopes and irregular topography that have little land leveling capacity and/or that require very efficient agricultural machinery, drip irrigation designs generally use pressure-compensating emitters. This work develops a methodology and implements it in a computer tool that makes it possible to optimally determine in drip-irrigated plots with pressure-compensating emitters: a) telescopic sizing of the submain manifold pipe, b) supply valve pressure and c) subunit''s intake valve location, when considering all hydraulic-economic aspects in the design phase. Techniques of optimal sizing of pipe networks and simulation of hydraulic networks under pressure are linked to economic analyzes of total annualized costs. Finally, the practical usefulness of the proposed methodology is shown with three examples of complex real cases where pipe design costs are reduced by 16-34% and energy costs by 37-51%

Characterization of an emergent clone of enteroinvasive Escherichia coli circulating in Europe

Enteroinvasive Escherichia coli (EIEC) cause intestinal illness indistinguishable from that caused by Shigella, mainly in developing countries. Recently an upsurge of cases of EIEC infections has been observed in Europe, with two large outbreaks occurring in Italy and in the United Kingdom. We have characterized phenotypically and genotypically the strains responsible for these epidemics together with an additional isolate from a sporadic case isolated in Spain. The three isolates belonged to the same rare serotype O96:H19 and were of sequence type ST-99, never reported before in EIEC or Shigella. The EIEC strains investigated possessed all the virulence genes harboured on the large plasmid conferring the invasive phenotype to EIEC and Shigella while showing only some of the known chromosomal virulence genes and none of the described pathoadaptative mutations. At the same time, they displayed motility abilities and biochemical requirements resembling more closely those of the non-pathogenic E. coli rather than the EIEC and Shigella strains used as reference. Our observations suggested that the O96:H19 strains belong to an emerging EIEC clone, which could be the result of a recent event of acquisition of the invasion plasmid by commensal E. coli

Current surgical concepts and indications in the management of the short bowel state: A call for the use of multidisciplinary intestinal rehabilitation programs

The mainstay of management for short bowel syndrome (SBS) is to promote access to the best quality of care provided by the intestinal rehabilitation program (IRP) in specialized centres. When treating SBS patients, the main goal is to minimize disease-associated complications, as well as achieve enteral autonomy. Surgical strategies should be selected cautiously upon the actual state of the bowel with respect to what it is clinically relevant for that specific patient. To this aim, a personalized and multidisciplinary approach for such a complex syndrome is needed

Mars Comm/Nav MicroSat Network

A recent Mars Exploration Program Architecture Definition Study, conducted by NASA with strong international participation, recommends establishment of a low cost in-situ communications and navigation relay satellite network to provide enabling and enhancing support for the international exploration of Mars. This would be the first step toward establishing a virtual presence throughout the solar system as called for in NASA\u27s Strategic Plan. The Mars satellite network concept, and its evolution from a prototype launched in 2003 to a full constellation, is described. Implementation of the Mars satellite network will utilize the common micromission bus being designed for piggyback launch by Ariane 5 as described in a companion paper, The Mars Micromissions Program. The requirements imposed on the common micromission bus to meet the needs of the Mars MicroSat network are discussed: A functional description is provided for the MicroSat payload, a UHF transceiver system, which supports the in-situ communications and navigation needs of user missions. Key technologies that are expected to play an important role in the implementation of the MicroSat network are also discussed