Guidelines for Designing Social Robots as Second Language Tutors

In recent years, it has been suggested that social robots have potential as tutors and educators for both children and adults. While robots have been shown to be effective in teaching knowledge and skill-based topics, we wish to explore how social robots can be used to tutor a second language to young children. As language learning relies on situated, grounded and social learning, in which interaction and repeated practice are central, social robots hold promise as educational tools for supporting second language learning. This paper surveys the developmental psychology of second language learning and suggests an agenda to study how core concepts of second language learning can be taught by a social robot. It suggests guidelines for designing robot tutors based on observations of second language learning in human–human scenarios, various technical aspects and early studies regarding the effectiveness of social robots as second language tutors

Enzymatic degradation of semi-dilute polymer solutions: coupling between enzyme mobility and activity

International audienceBiodegradable polymers (synthetic or natural) can be degraded by the action of living organisms. In most cases, biodegradation occurs through enzyme-catalysed depolymerisation, where enzymes hydrolyse bonds along the polymer chains. Lignocellulosic biomass (LC) is one of the most abundant renewable polymers that, when degraded, represents a tremendous source of interesting products. However, LC is difficult to degrade because plant cell wall polysaccharides are structurally and chemically complex molecules, forming a heterogeneous network of varying density and porosity. During industrial degradation, enzymes are confronted with LC substrates (e.g. wheat straws) that are insoluble and partially hydrated. They penetrate and propagate in the material, while at the same time changing its properties through hydrolysis. Although fascinating, little is known about the coupling between enzyme activity and mobility in the context of dense polymeric systems. In extremely tight polymer networks, the coupling is obvious: enzymes cannot just enter the polymer mesh and consequently move by 'eroding' the polymer surface [1]. In the more general case, enzymes can enter the network, but their activity is affected by the restricted diffusion in the polymer mesh [2]. Nevertheless, studies only consider the impact of the concentration of the polymer, neglecting its structural and dynamical evolution with hydrolysis time [3]; two characteristics that must have an effect on enzyme propagation in the material. Here we explore the coupling between enzyme mobility and activity by using a model polymer, arabinoxylan, extracted from wheat LC and a fluorescently labelled enzyme. We prepare semi-dilute polymer solutions that are concentrated at different mesh sizes. Then, the radial diffusion of the enzymes is observed using fluorescent microscopy. We present the results obtained with catalytically active and inactive enzymes. To complement these results, we present ex-situ experiments aiming at characterizing the polymer during the degradation (viscometry, SEC-MALS)

Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge

This review aims at answering the following question: how can a researcher be sure to succeed in grafting a protein onto a polymer surface? Even if protein immobilization on solid supports has been used industrially for a long time, hence enabling natural enzymes to serve as a powerful tool, emergence of new supports such as polymeric surfaces for the development of so-called intelligent materials requires new approaches. In this review, we introduce the challenges in grafting protein on synthetic polymers, mainly because compared to hard surfaces, polymers may be sensitive to various aqueous media, depending on the pH or reductive molecules, or may exhibit state transitions with temperature. Then, the specificity of grafting on synthetic polymers due to difference of chemical functions availability or difference of physical properties are summarized. We present next the various available routes to covalently bond the protein onto the polymeric substrates considering the functional groups coming from the monomers used during polymerization reaction or post-modification of the surfaces. We also focus our review on a major concern of grafting protein, which is avoiding the potential loss of function of the immobilized protein. Meanwhile, this review considers the different methods of characterization used to determine the grafting efficiency but also the behavior of enzymes once grafted. We finally dedicate the last part of this review to industrial application and future prospective, considering the sustainable processes based on green chemistry

Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge

This review aims at answering the following question: how can a researcher be sure to succeed in grafting a protein onto a polymer surface? Even if protein immobilization on solid supports has been used industrially for a long time, hence enabling natural enzymes to serve as a powerful tool, emergence of new supports such as polymeric surfaces for the development of so-called intelligent materials requires new approaches. In this review, we introduce the challenges in grafting protein on synthetic polymers, mainly because compared to hard surfaces, polymers may be sensitive to various aqueous media, depending on the pH or reductive molecules, or may exhibit state transitions with temperature. Then, the specificity of grafting on synthetic polymers due to difference of chemical functions availability or difference of physical properties are summarized. We present next the various available routes to covalently bond the protein onto the polymeric substrates considering the functional groups coming from the monomers used during polymerization reaction or post-modification of the surfaces. We also focus our review on a major concern of grafting protein, which is avoiding the potential loss of function of the immobilized protein. Meanwhile, this review considers the different methods of characterization used to determine the grafting efficiency but also the behavior of enzymes once grafted. We finally dedicate the last part of this review to industrial application and future prospective, considering the sustainable processes based on green chemistry

Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge

This review aims at answering the following question: how can a researcher be sure to succeed in grafting a protein onto a polymer surface? Even if protein immobilization on solid supports has been used industrially for a long time, hence enabling natural enzymes to serve as a powerful tool, emergence of new supports such as polymeric surfaces for the development of so-called intelligent materials requires new approaches. In this review, we introduce the challenges in grafting protein on synthetic polymers, mainly because compared to hard surfaces, polymers may be sensitive to various aqueous media, depending on the pH or reductive molecules, or may exhibit state transitions with temperature. Then, the specificity of grafting on synthetic polymers due to difference of chemical functions availability or difference of physical properties are summarized. We present next the various available routes to covalently bond the protein onto the polymeric substrates considering the functional groups coming from the monomers used during polymerization reaction or post-modification of the surfaces. We also focus our review on a major concern of grafting protein, which is avoiding the potential loss of function of the immobilized protein. Meanwhile, this review considers the different methods of characterization used to determine the grafting efficiency but also the behavior of enzymes once grafted. We finally dedicate the last part of this review to industrial application and future prospective, considering the sustainable processes based on green chemistry