Sull'antagonismo in vivo ed in vitro di Acremonium byssoides, endofita in Vitis vinifera, nei confronti di Plasmopara viticola

Lo studio dell\u2019interazione fra Acremonium byssoides, Vitis vinifera e Plasmopara viticola, condotto nell\u2019ultimo decennio, ha evidenziato in vitro e in vivo l\u2019attivit\ue0 antagonistica dell\u2019ifomicete, endofita negli organi verdi di alcune cultivars di vite, nei confronti del patogeno. In particolare, \ue8 stato accertato che sospensioni conidiche, filtrati colturali, estratti grezzi e metaboliti di A. byssoides riducono sensibilmente la germinazione delle spore agamiche e gamiche di P. viticola, limitando la produzione di propaguli. Inoltre, l\u2019uso di un microscopio laser confocale e l\u2019impiego di un\u2019opportuna tecnica di decolorazione dei tessuti fogliari, seguita da colorazione di contrasto, ha consentito di visualizzare l\u2019ifomicete, latente nelle nervature di foglie sane e iperparassita dell\u2019oomicete in foglie infette. In queste ultime, infatti, A. byssoides, dopo aver prodotto metaboliti secondari tossici per P. viticola, ne invade e degrada micelio, rami sporangiofori e spore gamiche. Tale attivit\ue0 antagonistica, determinando il contenimento sia della diffusione che della sopravvivenza del patogeno, pu\uf2 assumere, quindi, un ruolo rilevante nella definizione di strategie di difesa biologica contro la peronospora della vite

Structural changes of tissue samples exposed to low frequency electromagnetic field: A FT-IR absorbance study

In the present work, we report on a preliminary Fourier Transform Infrared (FT-IR) Absorbance study performed on different kind of rat tissues, such as kidney and heart, exposed to a "non-ionizing" radiation source at low frequency, in the range typical of micro-waves (300 MHz <v< 300 GHz). The data were collected in a wide wavenumber region, from 400 cm−1to 4000 cm−1. The comparison of the absorbance spectra in the case of the normal tissues with the irradiated ones has shown significant differences in the spectral features in accordance with the morphological analysis performed by the optical microscopy

Post-Mortem Immunohistochemical Evidence of β2-Adrenergic Receptor Expression in the Adrenal Gland

The evidence from post-mortem biochemical studies conducted on cortisol and catecholamines suggest that analysis of the adrenal gland could provide useful information about its role in human pathophysiology and the stress response. Authors designed an immunohistochemical study on the expression of the adrenal β2-adrenergic receptor (β2-AR), a receptor with high-affinity for catecholamines, with the aim to show which zones it is expressed in and how its expression differs in relation to the cause of death. The immunohistochemical study was performed on adrenal glands obtained from 48 forensic autopsies of subjects that died as a result of different pathogenic mechanisms using a mouse monoclonal β2-AR antibody. The results show that immunoreactivity for β2-AR was observed in all adrenal zones. Furthermore, immunoreactivity for β2-AR has shown variation in the localization and intensity of different patterns in relation to the original cause of death. To the best of our knowledge, this is the first study that demonstrates β2-AR expression in the human cortex and provides suggestions on the possible involvement of β2-AR in human cortex hormonal stimulation. In conclusion, the authors provide a possible explanation for the observed differences in expression in relation to the cause of death

Naphthalenone polyketides produced by Neofusicoccum parvum , a fungus associated with grapevine Botriosphaeria dieback

A strain of Neofusicoccum parvum isolated from declining vines was pathogenic to grapevine cultivar Inzolia in Sicily. This strain produced some metabolites in liquid medium. Crude extract, through a bio-guided purification process, yielded four naphthalenone polyketides. They were identified by comparison with spectroscopic data and optical proprieties reported in literature as: (3S, 4S)-7-ethyl-3,4,8-trihydroxy-6-methoxy-3,4-dihydro-1-(2H)-naphthalenone, (3S*, 4S*)-3,4-dihydro-3,4,8-trihydroxy-7-(1-hydroxyethyl)-6-methoxy-1-(2H)-naphthalenone, (4S)-3,4-dihydro-4,8-dihydroxy-1-(2H)-naphthalenone, named botryosphaerones D and A, isosclerone, respectively, and (3S*,4S*)-3,4,5-trihydroxy-1-tetralone (1-4). Phytotoxic activity of the isolated compounds (1-4) was tested on grapevine leaves at using the leaf puncture assay. All tested compounds were phytotoxic, with botryosphaerone D showing the greatest activity. The phytotoxic effects decreased when treated leaves were exposed to light. All of the metabolites did not show in vitro antifungal activity against Diplodia seriata, Lasiodiplodia mediterranea, Neofusicoccum vitifusiforme, or Phytophthora citrophthora. This is the first report of in vitro production of botryosphaerones D and A, and 3,4,5-trihydroxy-1-tetralone by N. parvum

Scaling Model of Annihilation-Diffusion Kinetics for Charged Particles with Long-Range Interactions

We propose the general scaling model for the diffusio n-annihilation reaction $A_{+} + A_{-} \longrightarrow \emptyset$ with long-range power-law i nteractions. The presented scaling arguments lead to the finding of three different regimes, dep ending on the space dimensionality d and the long-range force power e xponent n. The obtained kinetic phase diagram agrees well with existing simulation data and approximate theoretical results.Comment: RevTEX, 7 pages, no figures, accepted to Physical Review

Ruolo della timectomia nel trattamento della miastenia gravis: considerazioni e casistica personale

L’effetto terapeutico della timectomia sul decorso clinico della miastemia gravis è ancora quanto mai controverso. Infatti, mentre la chirurgia è ormai universalmente accettata per i timomi, il suo ruolo è ancora discutibile in pazienti con miastenia gravis. La via chirurgica di elezione per la timectomia totale è rappre - sentata dalla sternotomia mediana. Altre metodologie chirurgiche includono l’accesso cervicale e la sternotomia parziale. Queste tecni - che, seppure con alterne fortune, hanno mostrato risultati eccellenti nella exeresi del timo. Più recentemente la timectomia toracoscopica video-assistita è stata proposta come una tecnica meno invasiva e parimenti efficace per l’asportazione di quest’organo ed il trattamento della miastenia gravis. Scopo del presente lavoro è quello di riferire l’esperienza degli Autori in tema di timectomia, analizzando i dati riportati dalla lette - ratura internazionale sulla mortalità operatoria, le eventuali compli - canze e i risultati estetici delle diverse tipologie di accesso chirurgico

Dual inhibition of histone deacetylases and phosphoinositide 3-kinase enhances therapeutic activity against B cell lymphoma

Phosphoinositide 3-kinase (PI3K) and Myc are known to cooperate in promoting the survival and growth of a variety of B-cell lymphomas. While currently there are no small molecule inhibitors of Myc protein, histone deacetylase (HDAC) inhibitors have been shown to reduce levels of Myc protein by suppressing its transcription. We assessed the efficacy of CUDC-907, a new rationally designed dual inhibitor of PI3K and HDACs, in a panel of lymphoma cell lines. CUDC-907 treatment resulted in a dose- and time-dependent growth inhibition and cell death of DLBCL cell lines, irrespective of the cell of origin. CUDC-907 treatment down-regulated the phosphorylation of PI3K downstream targets, including AKT, PRAS40, S6, and 4EBP1, increased histone 3 acetylation, and decreased Myc protein levels. SILAC-based quantitative mass spectrometry demonstrated that CUDC-907 treatment decreased the protein levels of several components of the B cell receptor (BCR) and Toll like receptor (TLR) pathways, including BTK, SYK, and MyD88 proteins. These cellular changes were associated with an inhibition of NF-kB activation. CUDC-907 demonstrated in vivo efficacy with no significant toxicity in a human DLBCL xenograft mouse model. Collectively, these data provide a mechanistic rationale for evaluating CUDC-907 for the treatment of patients with Myc and PI3K-dependent lymphomas

Self-Consistent Model of Annihilation-Diffusion Reaction with Long-Range Interactions

We introduce coarse-grained hydrodynamic equations of motion for diffusion-annihilation system with a power-law long-range interaction. By taking into account fluctuations of the conserved order parameter - charge density - we derive an analytically solvable approximation for the nonconserved order parameter - total particle density. Asymptotic solutions are obtained for the case of random Gaussian initial conditions and for system dimensionality $d \geq 2$. Large-t, intermediate-t and small-t asymptotics were calculated and compared with existing scaling theories, exact results and simulation data.Comment: 22 pages, RevTEX, 1 PostScript figur

Green Extraction Approaches for Carotenoids and Esters: Characterization of Native Composition from Orange Peel

Abstract: Orange peel is a by-product produced in large amounts that acts as a source of natural pigments such as carotenoids. Xanthophylls, the main carotenoid class found in citrus fruit, can be present in its free form or esterified with fatty acids, forming esters. This esterification modifies the compound’s chemical properties, affecting their bioavailability in the human body, and making it important to characterize the native carotenoid composition of food matrices. We aimed to evaluate the non-saponified carotenoid extracts of orange peel (cv. Pera) obtained using alternative green approaches: extraction with ionic liquid (IL), analyzed by high performance liquid chromatography coupled to a diode array detector with atmospheric pressure chemical ionization and mass spectrometry HPLC-DAD-APCI-MS, and supercritical fluid extraction (SFE), followed by supercritical fluid chromatography with atmospheric pressure chemical ionization and triple quadrupole mass spectrometry detection (SFC-APCI/QqQ/MS) in an online system. Both alternative green methods were successfully applied, allowing the total identification of five free carotenoids, one apocarotenoid, seven monoesters, and 11 diesters in the extract obtained with IL and analyzed by HPLC-DAD-APCI-MS, and nine free carotenoids, six carotenoids esters, 19 apocarotenoids, and eight apo-esters with the SFE-SFC-APCI/QqQ/MS approach, including several free apocarotenoids and apocarotenoid esters identified for the first time in oranges, and particularly in the Pera variety, which could be used as a fruit authenticity parameter.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Bioscience Department, Universidade Federal de São Paulo, Rua Silva Jardin 136, 11015-020 Santos, BrazilDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Polo Annunziata, Viale Annunziata, 98168 Messina, ItalyDepartment of Mathematical and Computer Science, Physical Sciences and Earth Sciences, University of Messina, 98168 Messina, ItalyFederal Institute of São Paulo, Av. Clara Gianotti de Souza 5180, 11900-000 Registro, BrazilChemistry Department, Federal University of São Carlos, Rodovia Washington Luíz, Km 235, 13565-905 São Carlos, BrazilChromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyBeSep s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyUnit of Food Science and Nutrition, Department of Medicine, University Campus Bio-Medico of Rome, 00128 Rome, ItalyDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Via Consolare Valeria, 98125 Messina, ItalyBioscience Department, Universidade Federal de São Paulo, Rua Silva Jardin 136, 11015-020 Santos, BrazilFAPESP: 2015/26789-5FAPESP: 2016/18910-1FAPESP: 2017/20861-1FAPESP: 2019/25303-

Relaxation and Coarsening Dynamics in Superconducting Arrays

We investigate the nonequilibrium coarsening dynamics in two-dimensional overdamped superconducting arrays under zero external current, where ohmic dissipation occurs on junctions between superconducting islands through uniform resistance. The nonequilibrium relaxation of the unfrustrated array and also of the fully frustrated array, quenched to low temperature ordered states or quasi-ordered ones, is dominated by characteristic features of coarsening processes via decay of point and line defects, respectively. In the case of unfrustrated arrays, it is argued that due to finiteness of the friction constant for a vortex (in the limit of large spatial extent of the vortex), the typical length scale grows as $\ell_s \sim t^{1/2}$ accompanied by the number of point vortices decaying as $N_v \sim 1/t$. This is in contrast with the case that dominant dissipation occurs between each island and the substrate, where the friction constant diverges logarithmically and the length scale exhibits diffusive growth with a logarithmic correction term. We perform extensive numerical simulations, to obtain results in reasonable agreement. In the case of fully frustrated arrays, the domain growth of Ising-like chiral order exhibits the low-temperature behavior $\ell_q \sim t^{1/z_q}$, with the growth exponent $1/z_q$ apparently showing a strong temperature dependence in the low-temperature limit.Comment: 9 pages, 5 figures, to be published in Phys. Rev.