Effects of dietary inulin, statin, and their co-treatment on hyperlipidemia, hepatic steatosis and changes in drug-metabolizing enzymes in rats fed a high-fat and high-sucrose diet

<p>Abstract</p> <p>Background</p> <p>Rats fed a high-fat and high-sucrose (HF) diet develop hepatic steatosis and hyperlipidemia. There are several reports that a change in nutritional status affects hepatic levels of drug-metabolizing enzymes. Synthetic inulin is a dietary component that completely evades glucide digestion. Supplementing a HF diet with inulin ameliorates hypertriglycemia and hepatic steatosis, but not hypercholesterolemia. This study aimed at distinguishing the effects of synthetic inulin and 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (statin), which inhibit cholesterol biosynthesis.</p> <p>Methods</p> <p>We examined effects of co-treatment with synthetic inulin (5%) and fluvastatin (0, 4, and 8 mg/kg, <it>per os</it>) on body weight, epidydimal white adipose tissue weight, serum and hepatic lipid profiles, and hepatic cytochrome P450 (CYP) mRNA and protein profiles in rats fed a standard diet or a HF diet for 3 weeks.</p> <p>Results</p> <p>Treatment with the synthetic inulin (5%) or fluvastatin at 4 mg/kg (lethal dose in rats fed the HF diet, 8 mg/kg) ameliorated the elevation in hepatic triacylglycerol and total cholesterol levels in rats fed the HF diet. Whereas co-treatment with the inulin (5%) and fluvastatin (4 mg/kg) had a tendency to more strongly suppress the elevation in serum levels of very low density lipoprotein triacylglycerol than either treatment alone, no additive or synergistic effect was found in decrease in hepatic lipid levels. Hepatic levels of CYP1A1/2 and CYP2E1 mRNA and protein and methoxyresorufin <it>O</it>-demethylase and ethoxyresorufin <it>O</it>-deethylase activities were reduced in rats fed the HF diet. The synthetic inulin alleviated the reduction in hepatic levels of CYP1A1/2 and CYP2E1 mRNA and protein more strongly than fluvastatin, and no synergistic effects were observed on co-treatment. Furthermore, hepatic levels of aryl hydrocarbon receptor mRNA were decreased in rats fed the HF diet and recovered to near normal values with the intake of dietary inulin, which correlated with change in CYP1A1/2.</p> <p>Conclusions</p> <p>Dietary inulin alone was effective to prevent the development of hepatic steatosis, ameliorate nutritional effects, and alleviate the hepatic change in the expression of CYP1A1/2 and CYP2E1, while co-treatment with statin did not have additive or synergistic effects and statin may cause adverse effects in rats fed the HF diet.</p

Assessment of combined exposure to intermediate-frequency electromagnetic fields and pulsed electromagnetic fields among library workers in Japan

ObjectiveTo assess exposure levels to electromagnetic fields (EMFs) among library workers in Japan, focusing on co-exposure to intermediate-frequency EMF (IF-EMF) and pulsed EMF, to propose a new epidemiological research methodology.MethodsThe evaluated exposure sources were an electromagnetic type-electronic article surveillance gate (EM-EAS, IF-EMF (operating frequency 220 Hz-14 kHz)) and an activator/deactivator of anti-theft tags termed as “book check unit” (BCU, pulsed EMF). Short-term exposures were: (E1) whole-body exposure from the EAS gate when sitting within 3 m; (E2) local exposure to transient IF-EMF while passing through or beside the EAS gate; and (E3) local exposure to a pulsed magnetic field on BCU use. E1–E3 were evaluated based on exposure levels relative to magnetic flux density at the occupational reference level (RL; E1) or as per occupational basic restrictions (BR; E2 and E3) delineated by the International Commission on Non-Ionizing Radiation Protection (ICNIRP) 2010 guidelines. Exposure indices based on mid-term exposure (D1–D3), assuming exposure according to employment on a weekly basis, were used to assess exposure in actual working conditions. D1 represents continuous exposure from an EAS gate when sitting within 3 m of the gate. D2 and D3 represent repeated transient exposures occurring during gate pass or on the operation of a BCU. A link to a web-based questionnaire was distributed to librarians working at all libraries where the authors had mailed institutional questionnaires (4,073 libraries). Four exposure patterns were defined according to various exposure scenarios.ResultsWe obtained information on exposure parameters and working conditions from the 548 completed questionnaires. The ICNIRP guideline levels were not exceeded in any of the E1–E3 scenarios. Median of the D1 (% ICNIRP RL × hour/week) was 1, and &gt;85% respondents had values &lt;10. However, the maximum value was 513. Altogether, these results indicate that continuous exposure was low in most cases. The same tendency was observed regarding repeated transient exposure from EM-EAS gates (i.e., the median value for D2 (% ICNIRP BR × gate pass) was 5). However, there were several cases in which D1 and D2 values were &gt;10 times the median. The median of D3 (% ICNIRP BR × BCU operation) was 10, and most respondents' D3 values were greater than their D2 values, although the derived results depended on the assumptions made for the estimation.ConclusionWe conducted an assessment of combined exposures to IF-EMF and pulsed EMF among library workers in Japan by evaluating both short-term exposures (E1–E3) and exposure indices based on mid-term exposures (D1–D3) assuming actual working conditions per questionnaire results. These results provide useful information for future epidemiological studies

Polarimetric Imaging of Large Cavity Structures in the Pre-transitional Protoplanetary Disk around PDS 70: Observations of the disk

We present high resolution H-band polarized intensity (PI; FWHM = 0."1: 14 AU) and L'-band imaging data (FWHM = 0."11: 15 AU) of the circumstellar disk around the weak-lined T Tauri star PDS 70 in Centaurus at a radial distance of 28 AU (0."2) up to 210 AU (1."5). In both images, a giant inner gap is clearly resolved for the first time, and the radius of the gap is ~70 AU. Our data show that the geometric center of the disk shifts by ~6 AU toward the minor axis. We confirm that the brown dwarf companion candidate to the north of PDS 70 is a background star based on its proper motion. As a result of SED fitting by Monte Carlo radiative transfer modeling, we infer the existence of an optically thick inner disk at a few AU. Combining our observations and modeling, we classify the disk of PDS 70 as a pre-transitional disk. Furthermore, based on the analysis of L'-band imaging data, we put an upper limit mass of companions at ~30 to ~50MJ within the gap. Taking account of the presence of the large and sharp gap, we suggest that the gap could be formed by dynamical interactions of sub-stellar companions or multiple unseen giant planets in the gap.Comment: accepted by APJ

High-Resolution Submillimeter and Near-Infrared Studies of the Transition Disk around Sz 91

To reveal the structures of a transition disk around a young stellar object in Lupus, Sz 91, we have performed aperture synthesis 345 GHz continuum and CO(3--2) observations with the Submillimeter Array (\sim1\arcsec--3\arcsec resolution), and high-resolution imaging of polarized intensity at the $K_s$-band by using the HiCIAO instrument on the Subaru Telescope (0\farcs25 resolution). Our observations successfully resolved the inner and outer radii of the dust disk to be 65 AU and 170 AU, respectively, which indicates that Sz 91 is a transition disk source with one of the largest known inner holes. The model fitting analysis of the spectral energy distribution reveals an H$_2$ mass of $2.4\times10^{-3}$ M_\sun in the cold ($T<$30 K) outer part at $65<r<170$ AU by assuming a canonical gas-to-dust mass ratio of 100, although a small amount ($>3\times10^{-9}$ M_\sun) of hot ($T\sim$180 K) dust possibly remains inside the inner hole of the disk. The structure of the hot component could be interpreted as either an unresolved self-luminous companion body (not directly detected in our observations) or a narrow ring inside the inner hole. Significant CO(3--2) emission with a velocity gradient along the major axis of the dust disk is concentrated on the Sz 91 position, suggesting a rotating gas disk with a radius of 420 AU. The Sz 91 disk is possibly a rare disk in an evolutionary stage immediately after the formation of protoplanets because of the large inner hole and the lower disk mass than other transition disks studied thus far

High-Resolution Submillimeter and Near-Infrared Studies of the Transition Disk around Sz 91

To reveal the structures of a transition disk around a young stellar object in Lupus, Sz 91, we have performed aperture synthesis 345 GHz continuum and CO(32) observations with the Submillimeter Array ( 13 resolution), and high-resolution imaging of polarized intensity at the Ks-band by using the Hi-CIAO instrument on the Subaru Telescope (0.25 resolution). Our observations successfully resolved the inner and outer radii of the dust disk to be 65 and 170AU, respectively, which indicates that Sz 91 is a transition disk source with one of the largest known inner holes. The model fitting analysis of the spectral energy distribution reveals an H2 mass of 2.4 103 M in the cold (T 30 K) outer part at 65 r 170 AU by assuming a canonical gas-to-dust mass ratio of 100, although a small amount ( 3109 M) of hot (T 180 K) dust possibly remains inside the inner hole of the disk. The structure of the hot component could be interpreted as either an unresolved self-luminous companion body (not directly detected in our observations) or a narrow ring inside the inner hole. Significant CO(32) emission with a velocity gradient along the major axis of the dust disk is concentrated on the Sz 91 position, suggesting a rotating gas disk with a radius of 420 AU. The Sz 91 disk is possibly a rare disk in an evolutionary stage immediately after the formation of protoplanets because of the large inner hole and the lower disk mass than other transition disks studied thus far