Influencing factors on the suitability of organ-cultured corneas.

International audiencePURPOSE: To determine the factors related to donor and tissue retrieval, which influence the suitability of organ-cultured corneas for transplantation. PATIENTS AND METHODS: We retrospectively analysed 2596 donor corneas. Polytomic logistic regression analysis was used to assess the influence of various factors (that is, donor age, cause of death, death-to-tissue retrieval time, tissue retrieval-to-reception time, and tissue retrieval method) on the suitability of grafts for transplantation. Positive predictive values (PPVs) were computed. RESULTS: Forty-three percent (1118/2596) of corneas were discarded. The leading cause for discarding corneas was poor endothelial quality (21.5%). Corneas from donors older than 80 years were more likely to be discarded because of endothelial insufficiency (OR=2.37, P=0.001). Longer time between death and tissue retrieval was associated with increased risk of positive serology (OR=1.43, P=0.02). Increased time between tissue retrieval and reception was associated with increased risk of contamination (OR=1.57, P=0.03). PPV increased from 38.5% for corneas retrieved from donors older than 80 years featuring a death-to-tissue retrieval time of more than 6 h and a tissue retrieval-to-reception time of more than 24 h to 64.7% for corneas retrieved from donors younger than 80 years featuring a death-to tissue retrieval time shorter than 6 h and a tissue retrieval-to-reception time shorter than 24 h. CONCLUSION: The percentage of discarded corneas can be reduced by including donors aged 80 years or less, using a time from donor's death to tissue retrieval shorter than 6 h, and a tissue retrieval-to-reception time shorter than 24 h

Subgroup-specific structural variation across 1,000 medulloblastoma genomes.

Medulloblastoma, the most common malignant paediatric brain tumour, is currently treated with nonspecific cytotoxic therapies including surgery, whole-brain radiation, and aggressive chemotherapy. As medulloblastoma exhibits marked intertumoural heterogeneity, with at least four distinct molecular variants, previous attempts to identify targets for therapy have been underpowered because of small samples sizes. Here we report somatic copy number aberrations (SCNAs) in 1,087 unique medulloblastomas. SCNAs are common in medulloblastoma, and are predominantly subgroup-enriched. The most common region of focal copy number gain is a tandem duplication of SNCAIP, a gene associated with Parkinson's disease, which is exquisitely restricted to Group 4α. Recurrent translocations of PVT1, including PVT1-MYC and PVT1-NDRG1, that arise through chromothripsis are restricted to Group 3. Numerous targetable SCNAs, including recurrent events targeting TGF-β signalling in Group 3, and NF-κB signalling in Group 4, suggest future avenues for rational, targeted therapy

Production of strange B-baryons decaying into Xi-/+-l-/+ pairs at LEP

An excess of events containing, in a jet, a same-sign Xi(-/+) - l(-/+) pair as compared to those with an opposite-sign Xi(-/+) - l(-/+) pair has been observed in an analysis of 1.7 million hadronic Z(0) decays collected by the DELPHI detector at LEP between 1991 and 1993 inclusive. The probability for this signal to come from non B-baryon decays is less than 5 x 10(-4). The measured production fraction corresponds to: P(b --> B - baryon) x BR(B - baryon --> Xi(-) l(-) X) = (5.9 +/- 2.1 +/- 1.0) x 10(-4), per lepton species, averaged for electrons and muons and assuming the two channels have an equal contribution. Semileptonic decays of Lambda(b) baryons can account for less than 10% of these events and the major part of the signal has to originate from Xi(b) semileptonic decays, Using the subsample of these events where the Xi(-/+) trajectory has been measured in the Vertex Detector, the lifetime of B-baryons producing a Xi(-/+) in their semileptonic decay final state is found to be: tau(B - baryon --> Xi(-) l(-) X) = 1.5(-0.4)(+0.7) +/- 0.3 ps

PRODUCTION OF STRANGE B-BARYONS DECAYING INTO XI-/+-L-/+ PAIRS AT LEP

An excess of events containing, in a jet, a same-sign Xi(-/+) - l(-/+) pair as compared to those with an opposite-sign Xi(-/+) - l(-/+) pair has been observed in an analysis of 1.7 million hadronic Z(o) decays collected by the DELPHI detector at LEP between 1991 and 1993 inclusive. The probability for this signal to come from non B-baryon decays is less than 5 x 10(-4). The measured production fraction corresponds to: P(b --> B - baryon) x BR(B - baryon --> Xi(-) l(-) X) = (5.9 +/- 2.1 +/- 1.0) x 10(-4), per lepton species, averaged for electrons and muons and assuming the two channels have an equal contribution. Semileptonic decays of Lambda(b) baryons can account for less than 10% of these events and the major part of the signal has to originate from Xi(b) semileptonic decays, Using the subsample of these events where the Xi(-/+) trajectory has been measured in the Vertex Detector, the lifetime of B-baryons producing a Xi(-/+) in their semileptonic decay final state is found to be: tau(B - baryon --> Xi(-) l(-) X) = 1.5(-0.4)(+0.7) +/- 0.3 ps