Lentinus crinitus basidiocarp stipe and pileus: chemical composition, cytotoxicity and antioxidant activity

Lentinus crinitus is a wild fungus, which produces mushrooms consumed by some Amazonian Indians. Besides, it is recognized for its diverse biological activities and biotechnological applications. However, there are few reports with limited information on basidiocarp chemical composition and cytotoxicity. Our study determined and evaluated the chemical composition, cytotoxicity, and antioxidant activity of L. crinitus pileus and stipe separately. Chromatographic methods were used to evaluate basidiocarp chemical composition. Cytotoxicity was verified using a cell culture from porcine liver and against a panel of human tumor cells from different models. Antioxidant activity was assessed by different in vitro methods. The pileus had higher levels of protein, ash, tocopherols, and organic acids, mainly malic acid, than the stipe. The stipe revealed higher contents of carbohydrates, energy, soluble sugars, and phenolic acids, mostly p-hydroxybenzoic acid. L. crinitus basidiocarp has mainly trehalose as soluble sugar, and less than 1% fat being ~60% polyunsaturated fatty acids (mostly linoleic and oleic acids), and ~13% saturated fatty acids (mostly palmitic acid). L. crinitus revealed high antioxidant activity for most methods and no cytotoxic activity against tumor and non-tumor cells. L. crinitus basidiocarp can be considered a functional food with applicability in food, cosmetic, and pharmaceutical industries. Graphic abstract: [Figure not available: see fulltext.]This research was supported by Universidade Paranaense, UniCesumar, Polytechnic Institute of Bragança, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—finance code 001—, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação Araucária. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); to the national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros contract.info:eu-repo/semantics/publishedVersio

A model for drug dispensing service based on the care process in the Brazilian health system

Access to medication emphasizes the availability of the product at the expense of providing a service. The goal of this paper is to propose a theoretical model for a drug dispensing service, beginning with a reflection on the current realities of the Unified Health System and drug dispensation in Brazil. A conceptual analytical research made by a methodological course called disciplined imagination was mainly the approach applied to develop the model. The drug dispensing service is part of the care process, which considers access as an attribute; reception, connection and accountability, management, and clinical pharmaceutical aspects as components; and the rational use of drugs as the purpose. The proposed model addresses access to the dispensing service and demands a reorientation of routines, instruments, and practices.O acesso a medicamentos enfatiza a disponibilidade do produto em detrimento da provisão de um serviço. O objetivo deste trabalho é propor um modelo teórico para um serviço de dispensação de medicamentos, iniciando com uma reflexão sobre a realidade atual do Sistema Único de Saúde e a dispensação de medicamentos no Brasil. Uma pesquisa analítica conceitual realizada por meio de um percurso metodológico chamado de imaginação disciplinada constituiu a estratégia principal para o desenvolvimento do modelo. O serviço de dispensação é parte do processo de cuidado, o qual considera o acesso como um atributo; os aspectos acolhimento, vínculo e responsabilização, gestão e clínica farmacêutica como componentes e o uso racional de medicamentos como o propósito. O modelo proposto direciona o acesso para o serviço de dispensação e demanda a reorientação de rotinas, instrumentos e práticas

Boiling Points Of Short-chain Partial Acylglycerols And Tocopherols At Low Pressures By The Differential Scanning Calorimetry Technique

Boiling points of monocaprylin, monocaprin, dicaprylin, and dicaprin were measured at low pressures (1.0 kPa up to 13.2 kPa) using a differential scanning calorimetry technique and correlated by empirical equations (Clapeyron and Antoine). Parameters of the Design Institute for Physical Property Research (DIPPR) equations were also regressed considering calculated values of normal boiling points and critical points using the Marrero and Gani method. Heats of vaporization were estimated using the Clausius-Clapeyron relation with the inclusion of a correction term to account for deviations at higher pressures. Boiling points of α-, -, δ-, and γ-tocopherol were measured at 1.1 kPa. A DIPPR equation was also proposed for estimating the vapor pressure of this class of compounds, and an equation for predicting heat of vaporization was derived. 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