157 research outputs found
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats
PURPOSE: To evaluate the effects of copaiba oil ointment (Copaifera langsdorffii) on dorsal skin flaps in rats. METHODS: Adult male rats (n=30) were distributed into three groups of ten animals each, as follows: GC - control; GCA - absolute control and GT - treated with copaiba ointment. The rats were subjected to dorsal cutaneous skin flap surgery and the animals from the GC and GT received post-operative treatment for eight consecutive days. The animals from the GCA group did not receive treatment while the animals from the GC group received daily topical treatment of ointment without the active ingredient and the animals from the GT group were daily treated with 10% copaiba oil ointment. At the end of each experimental period the lesions were evaluated according to the percentage of necrotic area. Then, fragments from cranial, median and caudal parts were fixed in Boüin's solution and processed for paraffin embedding. The morphology of histological sections (5µm) was evaluated and the number of leucocytes, fibroblasts and blood vessels was also analyzed. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (pGC and GT), while the morphology showed larger granulation tissue with bulky fibroblasts and collagen fibers more arranged in the GT group. The morphometry showed a significant higher number of blood vessels in the median and caudal parts (GT>GCA and GC), leucocytes in the cranial part (GT>GC>GCA), and also fibroblasts in the median (GT and GC> GCA) and caudal parts (GT>GC and GCA) (p<0.05). CONCLUSION: The copaiba oil ointment favors angiogenesis and accelerates the viability of random skin flaps in rats.Federal Rural University of Pernambuco Department of Morphology and Animal Physiology Postgraduate Program in Animal BioscienceUFRPE Department of Morphology and Physiology Histology DivisionFederal University of Pernambuco Department of Morphology Histology DivisionFederal University of São Paulo Department of Morphology and Genetic Postgraduate Program in MorphologyUNIFESP, Department of Morphology and Genetic Postgraduate Program in MorphologySciEL
O PERFIL NUTRICIONAL DE ESCOLARES DE 6 a 10 ANOS DA REDE MUNICIPAL DA SEDE DE MUCAJAÍ – RORAIMA – BRASIL
Resumo: O objetivo do estudo foi identificar o Estado Nutricional de escolares de 06 a 10 anos da rede Municipal da Sede de Mucajaí-RR. Participaram 283 crianças (127 meninos e 156 meninas). A avaliação do estado nutricional foi realizada segundo os critérios de Warterlow (1976) empregando o padrão de referência do NCHS, por meio do software PED 2000. Para tanto, utilizou-se da estatística descritiva, distribuição em percentis e frequências. De acordo com as evidencias encontradas no presente estudo, percebe-se que os índices sobressaem para a prevalência de desnutrição (desnutrido crônico, desnutrido pregresso, desnutrido atual, 46,29%) sendo mais de quatro vezes superior a prevalência do excesso de peso (sobrepeso, obeso, grande obeso, 10,24%), e para o eutrofismo (43,46%). Quanto ao gênero, Verificou-se maior proporção de desnutrição no sexo feminino (47,44% vs 44,88%), excesso de peso mais elevado também no sexo feminino (13,46% vs 6,30%) e maior adequação nutricional no sexo masculino (48,82% vs 39,10%). Quanto ao gênero e idade, as evidências encontradas, apontam que a maior proporção de desnutridos encontra-se no sexo masculino na idade de 07 anos (56,26%), de excesso de peso no sexo feminino na idade de 9 anos (20,51%) e de eutróficos no sexo masculino na idade de 6 anos (68,00%). A partir do exposto conclui-se que os escolares da rede municipal da Sede de Mucajaí-RR, não estão em processo de transição nutricional como em todo país no que tange o binômio desnutrição-obesidade
Arqueologia e Comunidades na Gestão do Patrimônio Cultural - As Bibliotecas Vivas e o Resgate da História da (des)ocupação do Arruado do Engenho do Meio, Recife, PE
Trabalho apresentado no Simpósio 3 da VI Reunião da SAB Nordeste 202
Efeito da ivermectina sobre a ciclicidade, gestação e desenvolvimento neonatal em ratos
This work evaluates the estrous cycle, gestation and lactation of rats treated with ivermectin. Control (GI) rats received distilled water; and the remaining were treated during 45 days with ivermectin (GII=0.5; GIII=1.0; GIV=2.0; GV=4.0; GVI=8.0 and GVII=10.0 mg/kg) by the oral route, the drug being administered every three day, in a total of 15 administrations. Colpocytological examinations were then performed during 15 consecutive days. Four animals from each group were euthanized, and samples of ovaries and uteri were taken for histological evaluations. The remaining animals were mated and treated throughout the gestation period and further until the 15th postpartum day. Colpocytologic examinations revealed that the animals of groups II, III, IV, V, VI and VII presented a higher incidence of the estrus phase, in comparison with the control group (group I). Histopathologic studies showed that the groups treated with ivermectin presented an increased concentration of hyperplasic endometrial glands, whereas the morphology of the ovary was not altered. The treatment did not affect the gestation lenght, the number of newborns nor did it caused congenital mortality or newborns malformations. During lactation, there was a significant slowering of the body weight gain of the litter. It is concluded that the treatment of female rats with ivermectin increases the incidence of the estrus phase. In addition, a definite deleterious effect is exerted on nursing animals as revealed by the reduced body weight gain of the litter.O objetivo do presente trabalho foi avaliar o ciclo estral, gestação e lactação de ratas tratadas com ivermectina. 82 ratas albinas foram divididas em sete grupos. Os animais receberam água destilada (GI) ou diferentes doses de ivermectina, por via oral (GII=0,5; GIII=1,0; GIV=2,0; GV=4,0; GVI=8,0 e GVII=10,0 mg/kg). Os animais foram tratados por 45 dias, com ivermectina a cada três dias, totalizando 15 aplicações. Após esse período foi realizado exame colpocitológico durante 15 dias consecutivos. Ao final, quatro animais de cada grupo foram sacrificados e ovários e úteros retirados e processados para avaliação histológica. Os animais restantes foram acasalados e tratados com ivermectina, nas doses correspondentes a cada grupo, no 1º, 4º, 7º, 10º, 13º e 16º dias de gestação. Ao nascimento, os neonatos foram contados, analisados quanto à existência de defeitos congênitos, mortalidade e pesados até o 15º dia de lactação. Durante a lactação, as matrizes receberam novamente ivermectina no 1º, 4º, 7º, 10º e 13º dias. Nossos resultados mostraram que os animais dos grupos II, III, IV, V, VI e VII apresentaram maior incidência de estro em relação a GI. Quanto à histopatologia, os grupos tratados com a ivermectina apresentaram maior concentração de glândulas endometriais hiperplásicas. O tratamento não afetou tempo de gestação, número de neonatos, mortalidade ou defeitos congênitos. Na lactação observamos perda de peso na prole das matrizes tratadas com ivermectina. Pode-se concluir que a ivermectina, aumenta a incidência de estro e não deve ser indicada para uso em animais lactantes
Mast cell concentration and skin wound contraction in rats treated with Brazilian pepper essential oil (Schinus terebinthifolius Raddi)
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats
Microbial and fermentation profiles, losses and chemical composition of silages of buffel grass harvested at different cutting heights
Consultas neurológicas e diagnósticos em um grande hospital universitário dedicado a COVID-19
Background: More than one-third of COVID-19 patients present neurological symptomsranging from anosmia to stroke and encephalopathy. Furthermore, pre-existingneurological conditions may require special treatment and may be associated with worseoutcomes. Notwithstanding, the role of neurologists in COVID-19 is probablyunderrecognized. Objective: The aim of this study was to report the reasons forrequesting neurological consultations by internists and intensivists in a COVID-19-dedicated hospital. Methods: This retrospective study was carried out at Hospital dasClínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil, a 900-bedCOVID-19 dedicated center (including 300 intensive care unit beds). COVID-19 diagnosiswas confirmed by SARS-CoV-2-RT-PCR in nasal swabs. All inpatient neurologyconsultations between March 23rd and May 23rd, 2020 were analyzed. Neurologistsperformed the neurological exam, assessed all available data to diagnose theneurological condition, and requested additional tests deemed necessary. Difficultdiagnoses were established in consensus meetings. After diagnosis, neurologists wereinvolved in the treatment. Results: Neurological consultations were requested for 89 outof 1,208 (7.4%) inpatient COVID admissions during that period. Main neurologicaldiagnoses included: encephalopathy (44.4%), stroke (16.7%), previous neurologicaldiseases (9.0%), seizures (9.0%), neuromuscular disorders (5.6%), other acute brainlesions (3.4%), and other mild nonspecific symptoms (11.2%). Conclusions: Mostneurological consultations in a COVID-19-dedicated hospital were requested for severeconditions that could have an impact on the outcome. First-line doctors should be able torecognize neurological symptoms; neurologists are important members of the medicalteam in COVID-19 hospital care.Introdução: Mais de um terço dos pacientes com COVID-19 apresentam sintomasneurológicos que variam de anosmia a AVC e encefalopatia. Além disso, doençasneurológicas prévias podem exigir tratamento especial e estar associadas a pioresdesfechos. Não obstante, o papel dos neurologistas na COVID-19 é provavelmentepouco reconhecido. Objetivo: O objetivo deste estudo foi relatar os motivos para solicitarconsultas neurológicas por clínicos e intensivistas em um hospital dedicado à COVID-19. Métodos: Estudo retrospectivo realizado no Hospital das Clínicas da Faculdade deMedicina da Universidade de São Paulo, Brasil, um centro dedicado à COVID-19 com900 leitos (incluindo 300 leitos para unidades de terapia intensiva). O diagnóstico deCOVID-19 foi confirmado por SARS-CoV-2-RT-PCR em swabs nasais. Todas asinterconsultas de neurologia hospitalar entre 23 de março e 23 de maio de 2020 foramanalisadas. Os neurologistas realizaram o exame neurológico, avaliaram todos os dadosdisponíveis para diagnosticar a patologia neurológica e solicitaram exames adicionaisconforme necessidade. Diagnósticos difíceis foram estabelecidos em reuniões deconsenso. Após o diagnóstico, os neurologistas participaram da condução dos casos.Resultados: Foram solicitadas consultas neurológicas para 89 de 1.208 (7,4%) empacientes internados por COVID-19 durante o período. Os principais diagnósticosneurológicos incluíram: encefalopatia (44,4%), acidente vascular cerebral (16,7%),doenças neurológicas prévias (9,0%), crises epilépticas (9,0%), transtornosneuromusculares (5,6%), outras lesões encefálicas agudas (3,4%) e outros sintomasleves inespecíficos (11,2%). Conclusões: A maioria das consultas neurológicas em umhospital dedicado à COVID-19 foi solicitada para condições graves que poderiam afetaro desfecho clínico. Os médicos na linha de frente devem ser capazes de reconhecersintomas neurológicos. Os neurologistas são membros importantes da equipe médica noatendimento hospitalar à COVID-19
Evaluation of three recombinant proteins for the development of ELISA and immunochromatographic tests for visceral leishmaniasis serodiagnosis.
BACKGROUND Visceral Leishmaniasis (VL) is an infectious disease that is a significant cause of death among infants aged under
1 year and the elderly in Brazil. Serodiagnosis is a mainstay of VL elimination programs; however, it has significant limitations
due to low accuracy.
OBJECTIVE This study aimed to evaluate three recombinant Leishmania infantum proteins (rFc, rC9, and rA2) selected from previous
proteomics and genomics analyses to develop enzyme-linked immunosorbent assay (ELISA) and immunochromatographic tests
(ICT) for the serodiagnosis of human VL (HVL) and canine VL (CVL).
METHODS A total of 186 human (70 L. infantum-infected symptomatic, 20 other disease-infected, and 96 healthy) and 185
canine (82 L. infantum-infected symptomatic, 27 L. infantum-infected asymptomatic, and 76 healthy) sera samples were used for
antibody detection.
FINDINGS Of the three proteins, rA2 (91.5% sensitivity and 87% specificity) and rC9 (95.7% sensitivity and 87.5% specificity)
displayed the best performance in ELISA-HVL and ELISA-CVL, respectively. ICT-rA2 also displayed the best performance for
HVL diagnosis (92.3% sensitivity and 88.0% specificity) and had high concordance with immunofluorescence antibody tests
(IFAT), ELISA-rK39, IT-LEISH?, and ELISAEXT. ICT-rFc, ICT-rC9, and ICT-rA2 had sensitivities of 88.6%, 86.5%, and 87.0%,
respectively, with specificity values of 84.0%, 92.0%, and 100%, respectively for CVL diagnosis.
MAIN CONCLUSIONS The three antigens selected by us are promising candidates for VL diagnosis regardless of the test format,
although the antigen combinations and test parameters may warrant further optimisation
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