Arteriogenesis versus angiogenesis: similarities and differences

Cardiovascular diseases account for more than half of total mortality before the age of 75 in industrialized countries. To develop therapies promoting the compensatory growth of blood vessels could be superior to palliative surgical surgical interventions. Therefore, much effort has been put into investigating underlying mechanisms. Depending on the initial trigger, growth of blood vessels in adult organisms proceeds via two major processes, angiogenesis and arteriogenesis. While angiogenesis is induced by hypoxia and results in new capillaries, arteriogenesis is induced by physical forces, most importantly fluid shear stress. Consequently, chronically elevated fluid shear stress was found to be the strongest trigger under experimental conditions. Arteriogenesis describes the remodelling of pre-existing arterio-arteriolar anastomoses to completely developed and functional arteries. In both growth processes, enlargement of vascular wall structures was proposed to be covered by proliferation of existing wall cells. Recently, increasing evidence emerges, implicating a pivotal role for circulating cells, above all blood monocytes, in vascular growth processes. Since it has been shown that monocytes/macrophage release a cocktail of chemokines, growth factors and proteases involved in vascular growth, their contribution seems to be of a paracrine fashion. A similar role is currently discussed for various populations of bone-marrow derived stem cells and endothelial progenitors. In contrast, the initial hypothesis that these cells -after undergoing a (trans-)differentiation- contribute by a structural integration into the growing vessel wall, is increasingly challenged

Wie sich das Herz erneuert : von Umprogrammierungen und Stammzellen

Erkrankungen des Herz-Kreislauf-Systems sind für die größte Zahl der Todesfälle in Deutschland verantwortlich. Das liegt nicht zuletzt daran, dass das menschliche Herz kaum Selbstheilungskräfte besitzt. Wissenschaftler suchen deshalb nach Möglichkeiten, die Regenerationsfähigkeit des Organs zu steigern. Dabei helfen ihnen der Blick ins Tierreich und modernste molekularbiologische Verfahren

Improved arteriogenesis with simultaneous skeletal muscle repair in ischemic tissue by SCL plus multipotent adult progenitor cell clones from peripheral blood

Background: The CD34- murine stem cell line RM26 cloned from peripheral blood mononuclear cells has been shown to generate hematopoietic progeny in lethally irradiated animals. The peripheral blood-derived cell clones expresses a variety of mesodermal and erythroid/myeloid transcription factors suggesting a multipotent differentiation potential like the bone marrow-derived `multipotent adult progenitor cells' (MAP-C). Methods: SCL+ CD34- RM26 cells were transfused intravenously into mice suffering from chronic hind-limb ischemia, evaluating the effect of stem cells on collateral artery growth and simultaneous skeletal muscle repair. Results: RM26 cells are capable of differentiating in vitro into endothelial cells when cultured on the appropriate collagen matrix. Activation of the SCL stem cell enhancer (SCL+) is mediated through the binding to two Ets and one GATA site and cells start to express milieu- and growth condition-dependent levels of the endothelial markers CD31 (PECAM) and Flt-1 (VEGF-R1). Intravenously infused RM26 cells significantly improved the collateral blood flow (arteriogenesis) and neo-angiogenesis formation in a murine hind-limb ischemia transplant model. Although transplanted RM26 cells did not integrate into the growing collateral arteries, cells were found adjacent to local arteriogenesis, but instead integrated into the ischemic skeletal muscle exclusively in the affected limb for simultaneous tissue repair. Conclusion: These data suggest that molecularly primed hem-/mesangioblast-type adult progenitor cells can circulate in the peripheral blood improving perfusion of tissues with chronic ischemia and extending beyond the vascular compartment. Copyright (C) 2004 S. Karger AG, Basel

Resonances in Pulsatile Channel Flow with an Elastic Wall

Interactions between fluids and elastic solids are ubiquitous in application ranging from aeronautical and civil engineering to physiological flows. Here we study the pulsatile flow through a two-dimensional Starling resistor as a simple model for unsteady flow in elastic vessels. We numerically solve the equations governing the flow and the large-displacement elasticity and show that the system responds as a forced harmonic oscillator with non-conventional damping. We derive an analytical prediction for the amplitude of the oscillatory wall deformation, and thus the conditions under which resonances occur or vanish

Politische Bildung unter Bedingungen sozialer Distanz in der Corona-Krise: Lerngelegenheit, Herausforderung und neue Formate

Die Corona-Krise erzeugt bei Lernenden und Lehrenden sowie für ganze Professionen Lernnotwendigkeiten und -gelegenheiten. Gerade für die Politische Bildung ergeben sich dabei Chancen für subjekt- und konfliktorientierte Bildung. Gleichzeitig gilt es unter den Bedingungen der sozialen Distanz zu reflektieren, wie Bildung stattfinden kann. Dabei ist zu hinterfragen, inwiefern die neuen Lerngelegenheiten und -notwendigkeiten auch auf Visionen verweisen, die im Bildungsfeld angesteuert werden können. Der Beitrag beschreibt zunächst aus Sicht politischer Bildung die Corona-Krise als Lerngelegenheit. Anschließend wird der Einfluss der Krise auf das Bildungsfeld problematisiert. Dabei geht es um die Lernenden, die Lehrenden und die Pädagog*innen sowie die Strukturen, in denen die Akteur*innen agieren. Die Notwendigkeit, unter Bedingungen sozialer Distanz Bildungsprozesse zu initiieren, wird kritisch hinsichtlich der Möglichkeiten ihrer Verwirklichung reflektiert. Abschließend wird mit einem Schwerpunkt auf außerschulische Bildung danach gefragt, wie politische Bildung unter den Bedingungen sozialer Distanz gestaltet werden kann und wo sich Grenzen der Übertragbarkeit von Bildungssettings und -methoden in den digitalen Raum ergeben

Self-similar and disordered front propagation in a radial Hele-Shaw channel with time-varying cell depth

The displacement of a viscous fluid by an air bubble in the narrow gap between two parallel plates can readily drive complex interfacial pattern formation known as viscous fingering. We focus on a modified system suggested recently by [1], in which the onset of the fingering instability is delayed by introducing a time-dependent (power-law) plate separation. We perform a complete linear stability analysis of a depth-averaged theoretical model to show that the plate separation delays the onset of non-axisymmetric instabilities, in qualitative agreement with the predictions obtained from a simplified analysis by [1]. We then employ direct numerical simulations to show that in the parameter regime where the axisymmetrically expanding air bubble is unstable to nonaxisymmetric perturbations, the interface can evolve in a self-similar fashion such that the interface shape at a given time is simply a rescaled version of the shape at an earlier time. These novel, self-similar solutions are linearly stable but they only develop if the initially circular interface is subjected to unimodal perturbations. Conversely, the application of non-unimodal perturbations (e.g. via the superposition of multiple linearly unstable modes) leads to the development of complex, constantly evolving finger patterns similar to those that are typically observed in constant-width Hele-Shaw cells. [1] Z. Zheng, H. Kim, and H. A. Stone, Controlling viscous fingering using time-dependent strategies, Phys. Rev. Lett. 115, 174501 (2015).Comment: 12 pages, 8 figure

The Effect of Wall Inertia on High-Frequency Instabilities of Flow Through an Elastic-Walled Tube

We examine the effect of wall inertia on the onset of high-frequency self-excited oscillations in flow through an elastic-walled tube. The previous asymptotic model of Whittaker et al. (Proc. Roy. Soc. A466, 2010), for a long-wavelength high-frequency instability in a Starling-resistor set-up, neglected inertia in the tube wall. Here, we extend this model by modifying the ‘tube-law’ for the wall mechanics to include inertial effects. The resulting coupled model for the fluid and solid mechanics is solved to find the normal modes of oscillation for the system, together with their frequencies and growth rates. In the system and parameter regime considered, the addition of wall inertia reduces the oscillation frequency of each mode, however its effect on the stability of the system is not as straightforward. Increasing wall inertia lowers the mean flow rate required for the onset of instability, and is therefore destabilising. However, at higher flow rates the instability growth rate is decreased, and so wall inertia is stabilising here. Overall, the addition of wall inertia decreases the sensitivity of the system to the mean axial flow rate. The theoretical results show good qualitative and reasonable quantitative agreement with direct numerical simulations performed using the oomph-lib framework