Kaposi's sarcoma-associated herpesvirus oncoprotein K13 protects against B cell receptor induced growth arrest and apoptosis through NF-κB activation

Kaposi's sarcoma-associated herpesvirus (KSHV) has been linked to the development of Kaposi's sarcoma, primary effusion lymphoma and multicentric Castleman's disease (MCD). We have characterized the role of KSHV-encoded viral FLICE inhibitory protein K13 in the modulation of anti-IgM induced growth arrest and apoptosis in B cells. We demonstrate that K13 protects WEHI 231, an immature B cell line, against anti-IgM induced growth arrest and apoptosis. The protective effect of K13 was associated with the activation of the NF-κB pathway and was deficient in its mutant, K13-58AAA, and a structural homolog, vFLIP E8, which lack NF-κB activity. K13 upregulated the expression of NF-κB subunit RelB and blocked the anti-IgM induced decline in c-Myc and rise in p27(Kip1) that have been associated with growth arrest and apoptosis. K13 also upregulated the expression of Mcl-1, an anti-apoptotic member of the Bcl2 family. Finally, K13 protected the mature B cell line Ramos against anti-IgM induced apoptosis through NF-κB activation. Inhibition of anti-IgM induced apoptosis by K13 may contribute to the development of KSHV-associated lymphoproliferative disorders

Potencialidades do guandu como adubação verde em áreas cultivadas com cana-de-açúcar nos tabuleiros costeiros de Alagoas.

Grande parte da produção de cana-de-açúcar em Alagoas está concentrada nos tabuleiros costeiros, região litorânea com precipitação favorável ao desenvolvimento da cultura. Contudo, os solos de tabuleiros apresentam algumas limitações agrícolas referentes à baixa resistência mecânica a operações de preparo de solo, baixa fertilidade natural e de retenção de água e nutrientes. Os solos são caracterizados ainda por apresentarem uma alta coesão natural dos horizontes, o que dificulta a penetração e desenvolvimento radicular das plantas, a disponibilidade de água, a aeração e a absorção de nutrientes, podendo influenciar negativamente no desenvolvimento das culturas.bitstream/item/123063/1/Potencialidades-do-guandu.pd

Estimativas da diversidade genética de acessos de Paspalum spp. com ouso de marcadores microssatélites.

Editores técnicos: João de Mendonça Naime, Caue Ribeiro, Maria Alice Martins, Elaine Cristina Paris, Paulino Ribeiro Villas Boas, Ladislau Marcelino Rabello

Flow dynamics in a model of a dilated thoracic aorta prior to and following prosthetic replacement

We numerically investigate the flow dynamics in a model of a dilated thoracic aorta, and compare the flow features with the case of a prosthetic replacement in its ascending part. The flow is characterized by an inlet jet which impacts the aortic walls and sweeps toward the aortic arch. Secondary flows generated by the transvalvular jet evolve downstream into a helical flow. The small curvature radius at the end of the aortic arch induces flow separation and vortex shedding in the initial part of the descending aorta, during the systole. The implantation of a prosthesis determines several modifications in the global and local flow patterns. An increase of the pulse wave velocity in the aorta leads to larger pressures inside the vessel, due to the geometrical and rigidity modifications. The sweeping jet is more aligned along the axial direction and propagates faster along the aortic arch. Consequently, a stronger separation of the flow downstream of the aortic arch is observed. By also exploiting manifold analysis, we identify regions characterized by near-wall disordered flows which may present intense accumulation and drop of concentration of biochemicals. These regions are localized downstream of the prosthetic replacement, in the aortic arch, and may be more prone to a new emergence of vessel dilation

Sodium bicarbonate and time-to-exhaustion cycling performance: a retrospective analysis exploring the mediating role of expectancy

Background: A body of evidence has shown that ingesting 0.3 g·kg-1 body mass sodium bicarbonate (NaHCO3) can improve time-to-exhaustion (TTE) cycling performance, but the influence of psychophysiological mechanisms on ergogenic effects is not yet understood. Objective: This study retrospectively examined whether changes in TTE cycling performance are mediated by positive expectations of receiving NaHCO3 and/or decline in blood bicarbonate. Methods: In a randomised, crossover, counterbalanced, double-blind, placebo-controlled design, 12 recreationally trained cyclists (maximal oxygen consumption, 54.4 ± 5.7 mL·kg·min-1) performed four TTE cycling tests 90 min after consuming: i) 0.3 g·kg-1 body mass NaHCO3 in 5 mL·kg-1 body mass solution, ii) 0.03 g·kg-1 body mass sodium chloride in solution (placebo), iii) 0.3 g·kg-1 body mass NaHCO3 in capsules and iv) cornflour in capsules (placebo). Prior to exercise, participants rated on 1 – 5 Likert type scales how much they expected the treatment they believe had been given would improve performance. Capillary blood samples were measured for acid-base balance at baseline, pre-exercise and post-exercise. Results: Administering NaHCO3 in solution and capsules improved TTE compared with their respective placebos (solution: 27.0 ± 21.9 s, p = 0.001; capsules: 23.0 ± 28.1 s, p = 0.016). Compared to capsules, NaHCO3 administered via solution resulted in a higher expectancy about the benefits on TTE cycling performance (Median: 3.5 vs. 2.5, Z = 2.135, p = 0.033). Decline in blood bicarbonate during exercise was higher for NaHCO3 given in solution compared to capsules (2.7 ± 2.1 mmol·L-1, p = 0.001). Mediation analyses showed that improvements in TTE cycling were indirectly related to expectancy and decline in blood bicarbonate when NaHCO3 was administered in solution but not capsules

Desenvolvimento e aceitação de barra de cereal sabor umbu (Spondias tuberosa).

Produto bastante consumido nos dias atuais, a barra de cereal é uma alternativa saudável e prática. Este trabalho teve como objetivo formular barra de cereais contendo umbu, de preparo simples e baixo custo, e avaliar a aceitação do produto pelos consumidores, afim de propor a possível elaboração por pequenas agroindústrias. Foram desenvolvidas três formulações contendo ingredientes em diferentes proporções. A amostra 1 foi composta por xarope de polpa de umbu com sacarose, aveia prensada, coco ralado, castanha de caju, flocos de arroz, uva passa e doce de umbu em corte. A amostra 2 continha xarope de polpa de umbu com glicose, aveia prensada, coco ralado, castanha de caju, flocos de arroz e uva passa; e a amostra 3 foi composta por xarope caramelizado de polpa de umbu com sacarose, aveia prensada, coco ralado, castanha de caju, flocos de arroz e uva passa. Foi realizado teste de aceitação das barrinhas com 50 consumidores que avaliaram o quanto gostaram do produto utilizando escala hedônica de nove pontos (1=desgostei x extremamente; 9=gostei extremamente), além do grau de intenção de compra (1=certamente não compraria; 5=certamente compraria). Para aceitação da aparência, cor e textura as três formulações obtiveram notas sem diferença estatística (p < 0.05) entre si, e acima de 7, equivalente a ?gostei moderadamente? na escala. Para aroma, sabor e impressão global, as amostras 1 e 3 destacaram -se da amostra 2, sendo que a amostra 3 obteve as maiores médias de aceitação. Com relação ao sabor, a amostra 3 obteve média 8, equivalente a ?gostei muito? na escala. Segundo o teste de intenção de compra, 60% dos consumidores responderam que ?certamente comprariam? a amostra 3 caso estivesse a venda. Através desse trabalho pode-se concluir que as formulações testadas apresentaram bons resultados de aceitação, e que a formulação com xarope caramelizado foi a preferida pelos consumidores