Exploring the influence of competition on arbovirus invasion risk in communities

Arbovirus outbreaks in communities are affected by how vectors, hosts and non-competent species interact. In this study, we investigate how ecological interactions between species and epidemiological processes influence the invasion potential of a vector-borne disease. We use an eco-epidemiological model to explore the basic reproduction number R0 for a range of interaction strengths in key processes, using West Nile virus infection to parameterize the model. We focus our analysis on intra and interspecific competition between vectors and between hosts, as well as competition with non-competent species. We show that such ecological competition has non-linear effects on R0 and can greatly impact invasion risk. The presence of multiple competing vector species results in lower values for R0 while host competition leads to the highest values of risk of disease invasion. These effects can be understood in terms of how the competitive pressures influence the vector-to-host ratio, which has a positive relationship with R0. We also show numerical examples of how vector feeding preferences become more relevant in high competition conditions between hosts. Under certain conditions, non-competent hosts, which can lead to a dilution effect for the pathogen, can have an amplification effect if they compete strongly with the competent hosts, hence facilitating pathogen invasion in the community

In vitro inhibition of canine distemper virus by flavonoids and phenolic acids: Implications of structural differences for antiviral design

Infection caused by canine distemper virus (CDV) is a highly contagious disease with high incidence and lethality in the canine population. Antiviral activity of flavonoids quercetin, morin, rutin and hesperidin, and phenolic cinnamic, trans-cinnamic and ferulic acids were evaluated in vitro against the CDV using the time of addition assay to determine which step of the viral replicative cycle was affected. All flavonoids displayed great viral inhibition when they were added at the times 0 (adsorption) and 1 h (penetration) of the viral replicative cycle. Both quercetin and hesperidin presented antiviral activity at the time 2 h (intracellular). In the other hand, cinnamic acid showed antiviral activity at the times 0 and 2 h while trans-cinnamic acid showed antiviral effect at the times À1 h (pre-treatment) and 0 h. Ferulic acid inhibited CDV replicative cycle at the times 0 and 1 h. Our study revealed promising candidates to be consid- ered in the treatment of CDV. Structural differences among compounds and correlation to their antiviral activity were also explored. Our analysis suggest that these compounds could be useful in order to design new antiviral drugs against CDV as well as other viruses of great meaning in veterinary medicine

Constraining LLTB models with galaxy cluster counts from next generation surveys

International audienceThe Universe's assumed homogeneity and isotropy is known as the cosmological principle. It is one of the assumptions that lead to the Friedmann-Lemaître-Robertson-Walker (FLRW) metric and it is a cornerstone of modern cosmology, because the metric plays a crucial role into the determination of the cosmological observables. Thus, it is of paramount importance to question this principle and perform observational tests that may falsify this hypothesis. Here we explore the use of galaxy cluster counts as a probe of a large-scale inhomogeneity, which is a novel approach for the study of inhomogeneous models, and to determine the precision with which future galaxy cluster surveys will be able to test the cosmological principle. We present forecast constraints on the inhomogeneous Lemaître-Tolman-Bondi (LTB) model with a cosmological constant and cold dark matter, from a combination of simulated data according to a compilation of `Stage-IV' galaxy surveys following a methodology that involves the use of a mass function correction from numerical $N$-body simulations of an LTB cosmology. When considering the \lcdm fiducial model as a baseline for constructing our mock catalogs, we find that our combination of the forthcoming cluster surveys, will improve the constraints on the cosmological principle parameters as well on the FLRW parameters by about $50\%$ with respect to previous similar forecasts performed using geometrical and linear growth of structure probes, with $\pm20\%$ variations depending on the level of knowledge of systematic effects.These results indicate that galaxy cluster abundances are sensitive probes of inhomogeneity, and that next-generation galaxy cluster surveys, will thoroughly test homogeneity at cosmological scales, tightening the constraints on possible violations of the cosmological principle in the framework of $\Lambda$LTB scenarios. (Abridged

The Portuguese Society of Rheumatology position paper on the use of biosimilars - 2017 update

Biosimilars are new and more affordable similar versions of previously approved reference biological drugs. Following the approval of the first monoclonal antibody biosimilar in 2013, the Portuguese Society of Rheumatology issued a position paper on the use of biosimilars in rheumatic conditions covering efficacy, safety, extrapolation, interchangeability, substitution and pharmacovigilance. However, as this is a rapidly evolving field, it was felt that the knowledge and evidence gathered since then justified an update of these statements. Literature searches on these issues were performed and the search results were presented and discussed in a national meeting. Portuguese rheumatologists considered that affordability should be taken into consideration when initiating a biological drug, but other factors were equally important. In patients already on reference biological treatment, switch to a more affordable biosimilar is desirable, provided a set of conditions is rigorously met. Automatic substitution is not acceptable and current evidence is insufficient to support interchangeability. Extrapolation of clinical indications is endorsed by Portuguese rheumatologists, and the statements on safety, pharmacovigilance and traceability are in accordance with the previous position paper.publishersversionpublishe

Fixação biológica de nitrogênio e teores foliares de nutrientes na soja em função de doses de molibdênio e gesso agrícola Biological nitrogen fixation and leaf nutrient concentration on soybean as a function of molybdenum and gypsum levels

A competitividade econômica da soja brasileira no mercado mundial se deve, em grande parte, aos benefícios da fixação biológica do nitrogênio na cultura. O trabalho foi conduzido a campo, sob sistema plantio direto, em condição de sequeiro, no Município de Maracaju-MS, com o objetivo de avaliar a nodulação, o crescimento, nutrição mineral e produtividade de grãos da soja submetida a diferentes doses de gesso agrícola e molibdênio. Foi utilizado o delineamento experimental de blocos ao acaso, com cinco repetições e esquema de parcelas subdivididas, sendo as parcelas representadas por quatro doses de gesso agrícola (0, 1.000, 2.000 e 3.000 kg ha-1) e as subparcelas, por quatro doses de molibdênio (0, 20, 40 e 60 g ha-1). Não houve efeito da interação gesso x Mo sobre a produtividade da soja. O gesso agrícola não influencia no teor foliar de N e na produtividade. O Mo proporciona incrementos na produtividade e no teor de proteínas dos grãos.<br>The economic competitiveness of Brazilian soybeans on the world market occurs, in large part, due to the benefits of biological nitrogen fixation in this crop. The field experiment was carried out in Maracaju, Mato Grosso do Sul State, Brazil, under no-tillage system, in rainfed condition. The aim was to evaluate nodulation, growth, mineral nutrition and grain yield of soybeans under different doses of gypsum and molybdenum. The experimental design used was a randomized block with five replicates and arranged in a split-plot squeme, with the plot represented by four doses of gypsum (0, 1.000, 2.000 and 3.000 kg ha-1) and the subplots by four doses of molybdenum (0, 20, 40 and 60 g ha-1). There were no interaction effects of Mo x gypsum for grain yield. Gypsum has no influence in the N leaf content and grain yield. Mo increases grain yield and protein levels in the grain