A novel species of mastrevirus (family Geminiviridae) isolated from Digitaria didactyla grass from Australia.

Mastreviruses (family Geminiviridae) that infect monocotyledonous plants occur throughout the temperate and tropical regions of Asia, Africa, Europe and Australia. Despite the identification of a very diverse array of mastrevirus species whose members infect African monocots, few such species have been discovered in other parts of the world. For example, the sequence of only a single monocot-infecting mastrevirus, Chloris striate mosaic virus (CSMV), has been reported so far from Australia, even though earlier biological and serological studies suggested that other distinct mastreviruses were present. Here, we have obtained the complete nucleotide sequence of a virus from the grass Digitaria didactyla originating from Australia. Analysis of the sequence shows the virus to be a typical mastrevirus, with four open reading frames, two in each orientation, separated by two non-coding intergenic regions. Although it showed the highest levels of sequence identity to CSMV (68.7%), their sequences are sufficiently diverse for the virus to be considered a member of a new species in the genus Mastrevirus, based on the present species demarcation criteria. We propose that the name first used during the 1980s be used for this species, Digitaria didactyla striate mosaic virus (DDSMV)

Nutritional intervention during gestation alters growth, body composition and gene expression patterns in skeletal muscle of pig offspring

peer-reviewedVariations in maternal nutrition during gestation can influence foetal growth, foetal development and permanently ‘programme’ offspring for postnatal life. The objective of this study was to analyse the effect of increased maternal nutrition during different gestation time windows on offspring growth, carcass quality, meat quality and gene expression in skeletal muscle. A total of 64 sows were assigned to the following feeding treatments: a standard control diet at a feed allocation of 2.3 kg/day throughout gestation, increased feed allowance of 4.6 kg/day from 25 to 50 days of gestation (dg), from 50 to 80 dg and from 25 to 80 dg. At weaning, Light, Medium and Heavy pigs of the same gender, within litter, were selected based on birth weight, individually penned and monitored until slaughter at 130 days post weaning. Carcass and meat quality traits of the semimembranosus (SM) muscle were recorded post mortem. A cross section of the semitendinosus (ST) muscle encompassing the deep and superficial regions were harvested from pigs (n518 per treatment) for RNA extraction and quantification of gene expression by real-time PCR. The results showed that doubling the feed intake from 25 to 50 dg reduced offspring growth, carcass weight, intramuscular fat content and increased drip loss of the SM muscle. Interestingly, protein phosphatase 3 catalytic subunit – a-isoform, which codes for the transcription factor calcineurin, was upregulated in the ST muscle of offspring whose mothers received increased feed allowance from 25 to 50 dg. This may provide an explanation for the previous observed increases in Type IIa muscle fibres of these offspring. Increasing the maternal feed intake from 50 to 80 dg negatively impacted pig growth and carcass weight, but produced leaner male pigs. Extending the increased maternal feed intake from 25 to 80 dg had no effect on offspring over the standard control gestation diet. Although intra-litter variation in pig weight is a problem for pig producers, increased maternal feeding offered no improvement throughout life to the lighter birth weight littermates in our study. Indeed, increased maternal nutrition at the three-gestation time windows selected provided no major benefits to the offspring.Teagasc, under the National Development Plan; Teagasc Walsh Fellowship; Short Term Scientific Mission (STSM) fund, COST925

Identification of DNA Components Required for Induction of Cotton Leaf Curl Disease

AbstractCotton leaf curl disease (CLCuD) is a major constraint to cotton production in Pakistan. Infectious clones of the monopartite begomovirus cotton leaf curl virus (CLCuV), associated with diseased cotton, are unable to induce typical symptoms in host plants. We have identified and isolated a single-stranded DNA molecule approximately 1350 nucleotides in length which, when coinoculated with the begomovirus to cotton, induces symptoms typical of CLCuD, including vein swelling, vein darkening, leaf curling, and enations. This molecule (termed DNA β) requires the begomovirus for replication and encapsidation. The CLCuV/DNA 1/DNA β complex, together with a similar complex previously identified in Ageratum conyzoides, represent members of an entirely new type of infectious, disease-causing agents. The implications of this finding to our understanding of the evolution of new disease-causing agents are discussed

Bromus catharticus striate mosaic virus: a new mastrevirus infecting Bromus catharticus from Australia.

Although monocotyledonous-plant-infecting mastreviruses (in the family Geminiviridae) are known to cause economically significant crop losses in certain areas of the world, in Australia, they pose no obvious threat to agriculture. Consequently, only a few Australian monocot-infecting mastreviruses have been described, and only two have had their genomes fully sequenced. Here, we present the third full-genome sequence of an Australian monocot-infecting mastrevirus from Bromus catharticus belonging to a distinct species, which we have tentatively named Bromus catharticus striate mosaic virus (BCSMV). Although the genome of this new virus shares only 57.7% sequence similarity with that of its nearest known relative, Digitaria didactyla striate mosaic virus (DDSMV; also from Australia), it has features typical of all other known mastrevirus genomes. Phylogenetic analysis showed that both the full genome and each of its probable expressed proteins group with the two other characterised Australian monocot-infecting mastreviruses. Besides the BCSMV genome sequence revealing that Australian monocot-infecting mastrevirus diversity rivals that seen in Africa, it has enabled us, for the first, to time detect evidence of recombination amongst the Australian viruses. Specifically, it appears that DDSMV possesses a short intergenic region sequence that has been recombinationally derived from either BCSMV or a close relative that has not yet been identified

The (pol.p,2p) Reaction on 2-H, 3,4-He, and 40-Ca at 200 MeV

This research was sponsored by the National Science Foundation Grant NSF PHY-931478

Sazonalidade de variáveis biofísicas em regiões semiáridas pelo emprego do sensoriamento remoto Biophysics variables seasonality on surface in semiarid regions by using remote sensing

Para investigar alterações no albedo, no Índice de Vegetação por Diferença Normalizada (NDVI), no saldo de radiação e no fluxo de calor no solo, em decorrência do regime pluviométrico no semiárido cearense, desenvolveu-se um estudo na bacia do Rio Trussu - Ceará, empregando-se sensoriamento remoto. Foram utilizadas duas imagens Landsat 7 ETM+, datadas de 25-10-2000 e 24-7-2001, sendo as variáveis estimadas pelo emprego do algoritmo SEBAL (Surface Energy Balance Algorithms for Land). Os resultados mostraram que as variáveis investigadas apresentaram alterações entre as duas estações, sendo os maiores valores de albedo registrados na estação seca. O NDVI apresentou maior sensibilidade ao regime hídrico, mostrando alto potencial de recuperação da vegetação ao efeito da precipitação. As margens do Rio Trussu apresentaram NDVI superior a 0,39, sendo indicativo de preservação da mata ciliar. A vegetação da bacia mostrou alto poder resiliente expresso pelo incremento nos valores de NDVI para o ano de 2001. A estação chuvosa exerceu também influência marcante sobre o saldo de radiação e fluxo de calor no solo, confirmando o efeito da estação climática na modificação do balanço de energia sobre a bacia.<br>To investigate the rainfall regime effects over the albedo, NDVI (normalized difference vegetation index), net radiation and soil heat flux in a semiarid region (Northeast of Brazil), a study in the Trussu watershed was developed by using remote sensing. The study focuses on two images (Landsat 7 ETM+) provided by Instituto Nacional de Pesquisas Espaciais (INPE), from October 25, 2000 and July 24, 2001, each of them having a different rainfall regime (dry and wet seasons). The images were analyzed by using the SEBAL algorithm (surface energy balance algorithm for land). The results showed that the amount of rainfall affected the investigated variables, and the highest values of albedo were registered during the dry season. The NDVI presented high sensibility to rainfall regime, pointing out a high vegetation potential recover during the rainfall season. The NDVI along the Trussu River was up to 0.39, expressing the repair zone preservation. The watershed vegetation showed a high resilience power expressed by NDVI values in the year of 2001. Net radiation and soil heat flux were greater in the dry season, in this way expressing the effect of humidity on the energy balance