WAVELET BASED NONLINEAR SEPARATION OF IMAGES

This work addresses a real-life problem corresponding to the separation of the nonlinear mixture of images which arises when we scan a paper document and the image from the back page shows through. The proposed solution consists of a non-iterative procedure that is based on two simple observations: (1) the high frequency content of images is sparse, and (2) the image printed on each side of the paper appears more strongly in the mixture acquired from that side than in the mixture acquired from the opposite side. These ideas had already been used in the context of nonlinear denoising source separation (DSS). However, in that method the degree of separation achieved by applying these ideas was relatively weak, and the separation had to be improved by iterating within the DSS scheme. In this paper the application of these ideas is improved by changing the competition function and the wavelet transform that is used. These improvements allow us to achieve a good separation in one shot, without the need to integrate the process into an iterative DSS scheme. The resulting separation process is both nonlinear and non-local. We present experimental results that show that the method achieves a good separation quality

Construção de uma Bateria de Problemas de Julgamento e Decisão em Língua Portuguesa

O nosso principal objetivo foi construir uma bateria de problemas de julgamento e decisão em condições de incerteza que possa ser utilizada pela investigação feita em português. A importância deste trabalho passa por construir material cuja qualidade é controlada de forma a reduzir os erros de medida associados à estrutura superficial dos problemas utilizados e, ao mesmo tempo, garantir que os resultados obtidos resultam realmente das características dos processos de julgamento. Com efeito, só desta forma é possível integrar novos dados nas atuais perspetivas de julgamento e decisão e contribuir para o desenvolvimento desta área de investigação. Para além da adaptação de problemas existentes em língua inglesa e da criação de novos itens, foram igualmente criadas versões em que existe conflito entre respostas alternativas e versões em que estas respostas convergem. Para garantir a qualidade desta nova bateria o desempenho de 117 participantes em cada uma das questões foi analisado. A bateria é composta por itens do Teste de reflexão cognitiva (Cognitive Reflection Test), problemas de raciocínio disjuntivo, silogismos, problemas de viés com base no resultado (outcome bias) e de viés retrospetivo (hidsight bias), problemas de probabilidades de partida (base rates e base rates causais), problemas de conjunção de probabilidades e, também, problemas de falácia do jogador

Identification of protein-coding and non-coding RNA expression profiles in CD34+ and in stromal cells in refractory anemia with ringed sideroblasts

<p>Abstract</p> <p>Background</p> <p>Myelodysplastic syndromes (MDS) are a group of clonal hematological disorders characterized by ineffective hematopoiesis with morphological evidence of marrow cell dysplasia resulting in peripheral blood cytopenia. Microarray technology has permitted a refined high-throughput mapping of the transcriptional activity in the human genome. Non-coding RNAs (ncRNAs) transcribed from intronic regions of genes are involved in a number of processes related to post-transcriptional control of gene expression, and in the regulation of exon-skipping and intron retention. Characterization of ncRNAs in progenitor cells and stromal cells of MDS patients could be strategic for understanding gene expression regulation in this disease.</p> <p>Methods</p> <p>In this study, gene expression profiles of CD34⁺cells of 4 patients with MDS of refractory anemia with ringed sideroblasts (RARS) subgroup and stromal cells of 3 patients with MDS-RARS were compared with healthy individuals using 44 k combined intron-exon oligoarrays, which included probes for exons of protein-coding genes, and for non-coding RNAs transcribed from intronic regions in either the sense or antisense strands. Real-time RT-PCR was performed to confirm the expression levels of selected transcripts.</p> <p>Results</p> <p>In CD34⁺cells of MDS-RARS patients, 216 genes were significantly differentially expressed (q-value ≤ 0.01) in comparison to healthy individuals, of which 65 (30%) were non-coding transcripts. In stromal cells of MDS-RARS, 12 genes were significantly differentially expressed (q-value ≤ 0.05) in comparison to healthy individuals, of which 3 (25%) were non-coding transcripts.</p> <p>Conclusions</p> <p>These results demonstrated, for the first time, the differential ncRNA expression profile between MDS-RARS and healthy individuals, in CD34⁺cells and stromal cells, suggesting that ncRNAs may play an important role during the development of myelodysplastic syndromes.</p

Surface characterization of Ti-Si-C-ON coatings for orthopedic devices : XPS and Raman spectroscopy

Ti–Si–C–ON films were deposited by DC reactive magnetron sputtering and their chemical properties, biofilm formation and toxicity were characterized. Based on the films composition three different growth regimes were identified on the films; (I) N/Ti = 2.11 (high atomic ratio) and low oxygen content; (II) 0.77 ≤ N/Ti ≤ 1.86 (intermediate atomic ratio) and (III) N/Ti ≤ 0.12 (low ratio) and high oxygen content. The phase composition varied from mainly TiN on regime I to TiCN on regime 2 and titanium oxides on regime III. Taking into account the results of biological characterization (biofilm formation and cytotoxicity), it was possible to conclude that samples with a high TiN content (regime I) presented more favorable biocompatibility, since it was less prone to microbial colonization and also displayed a low cytotoxicity.The authors are grateful to Dr. Alicia Andres, Institut de Ciencia de Materiales de Madrid (ICMM-CSIC), for his assistance in carrying out the Raman spectroscopic analysis. The work was financially supported by the CRUP Institution (project "Accao No E-1007/08), and the Spanish Ministry of Science and Innovation (projects FUN-COAT CSD2008-00023, MAT2008-06618-C02 and Integrated action HP016-2007). This research is partially sponsored by FEDER funds through the program COMPETE- Programa Operacional Factores de Competitividade and by Portuguese national funds through FCT-Fundacao para a Ciencia e a Tecnologia, under the project PTDC/CTM/102853/2008

Human phenylalanine hydroxylase as the case study

Funding Information: Authors acknowledge Sofarimex, Indústria Química e Farmacêutica SA, Portugal, for all the support concerning freeze-drying studies. This work was supported by FEDER and Fundação para a Ciência e a Tecnologia, I. P. through iMED.ULisboa (Projects UIDB/04138/2020 and UIDP/04138/2020), iNOVA4Health (UIDB/04462/2020, UIDP/04462/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020) and research project PTDC/EBB-BIO/101237/2008 and research grant SFRH/BD/47946/2008 (to Paulo R. Lino). This work has also received funding from the National PKU Alliance, USA. The authors would like to thank Luís Miguel Ramos and Cátia Nascimento who contributed to the exploratory research that culminated in the work herein presented. Funding Information: Authors acknowledge Sofarimex, Indústria Química e Farmacêutica SA, Portugal, for all the support concerning freeze-drying studies. This work was supported by FEDER and Fundação para a Ciência e a Tecnologia, I. P. through iMED.ULisboa (Projects UIDB/04138/2020 and UIDP/04138/2020), iNOVA4Health (UIDB/04462/2020, UIDP/04462/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020) and research project PTDC/EBB-BIO/101237/2008 and research grant SFRH/BD/47946/2008 (to Paulo R. Lino). This work has also received funding from the National PKU Alliance, USA. Publisher Copyright: © 2023 The Author(s)The structural maintenance of therapeutic proteins during formulation and/or storage is a critical aspect, particularly for multi-domain and/or multimeric proteins which usually exhibit intrinsic structural dynamics leading to aggregation with concomitant loss-of-function. Protein freeze-drying is a widely used technique to preserve protein structure and function during storage. To minimize chemical/physical stresses occurring during this process, protein stabilizers are usually included, their effect being strongly dependent on the target protein. Therefore, they should be screened for on a time-consuming case-by-case basis. Herein, differential scanning fluorimetry (DSF) and isothermal denaturation fluorimetry (ITDF) were employed to screen, among different classes of freeze-drying additives, for the most effective stabilizer of the model protein human phenylalanine hydroxylase (hPAH). Correlation studies among retrieved DSF and ITDF parameters with recovered enzyme amount and activity indicated ITDF as the most appropriate screening method. Biochemical and biophysical characterization of hPAH freeze-dried with ITDF-selected stabilizers and a long-term storage study (12 months, 5 ± 3 °C) showed that the selected compounds prevented protein aggregation and preserved hPAH structural and functional properties throughout time storage. Our results provide a solid basis towards the choice of ITDF as a high-throughput screening step for the identification of protein freeze-drying protectors.publishersversionpublishe

Influence of the surface morphology and microstructure on the biological properties of Ti-Si-C-N-O coatings

Detailed structural, microstructural, biofilm formation and cytotoxicity studies were performed on Ti-Si-C-ON hard coatings prepared by dc reactive magnetron sputtering, in order to evaluate the relation among these properties. Compositional analysis showed the existence of two distinct regimens; regime I: high C/Si atomic ratio (C/Si ≥1,42) and intermediate N/Ti atomic ratio; regime II: low C/Si atomic ratio (C/Si≤0, 49) and low N/Ti atomic ratio. The structural analysis revealed that, in regime I, films crystallized in a B1-NaCl crystal structure typical of TiC0.2 N0.8. In regime II, the decrease of C/Si and increase in silicon concentration led to the formation of Ti-Si-CON along with a reduction of grain size in the films. Atomic Force Microscopy observations showed that the surface morphology of these Ti-Si-C-ON films became smoother when the silicon content increased and the nitrogen content decreased, which is consistent with the formation of nanosized clusters. Concerning biological properties, it was observed that cytotoxicity could be related with the titanium concentration while biofilm formation ability was found to be related with the surface morphology of the films.The authors are grateful to Dr. Alicia Andres, Instituto de Ciencia de Materiales de Madrid (ICMM-CSIC), for her assistance in carrying out the Raman spectroscopic analysis. The project was financially supported by the CRUP Institution (project "Accao N0 E-1007/08"), and the Spanish Ministry of Science and Innovation (projects FUNCOAT CSD2008-00023 and HP2007-0116)

XRD and FTIR analysis of Ti–Si–C–ON coatings for biomedical applications

Ti–Si–C–ON films were deposited by DC reactive magnetron sputtering using different partial pressure ratio of oxygen (pO2) and nitrogen (pN2). Compositional analysis revealed the existence of three different growth zones for the films; (I) N/Ti = 2.1 (high atomic ratio) and low oxygen content; (II) 0.76 < N/Ti < 2.1 (intermediate atomic ratio) and (III) N/Ti ≤ 0.12 (low ratio) and high oxygen content. For high N/Ti atomic ratio (N/Ti = 2.1) the XRD pattern exhibits reflections that correspond to a mixture of two different phases: a metallic-like Ti and a fcc NaCl type structure. Its electrical resistivity presents a metallic character and, consequently, has high infrared reflectivity. For the intermediate N/Ti ratio (0.76 < N/Ti < 2.1), the films crystallize in a B1-NaCl crystal structure typical for TiC0.2N0.8. Their FTIR spectra present C–N modes, besides the TiN ones, that indicate a progressive substitution of nitrogen by carbon atoms with increasing oxygen content (and lowering N/Ti ratio). For the highest oxygen content (and lower N/Ti ratio) the presence of the Ti–O–Ti stretching mode shows the formation of highly resistive Ti–O compounds consistent with the semiconductor character of this film. Biofilm formation as well as material cytotoxicity seemed to be related with the presence of the Ti

Numerical and experimental characterisation of polylactic acid (PLA) processed by additive manufacturing (AM): bending and tensile tests

In additive manufacturing (AM), one of the most popular procedures is material extrusion (MEX). The materials and manufacturing parameters used in this process have a significant impact on a printed product’s quality. The purpose of this work is to investigate the effects of infill percentage and filament orientation on the mechanical properties of printed structures. For this reason, the characterisation of polylactic acid (PLA) was done numerically using the finite element method and experimentally through mechanical tests. The experiments involved three-point bending and tensile tests. The results showed that mechanical performance is highly dependent on these processing parameters mainly when the infill percentage is less than 100%. The highest elastic modulus was exhibited for structures with filament align at 0◦ and 100% infill, while the lowest one was verified for specimen filament aligned at 0◦ and 30% infill. The results demonstrated that the process parameters have a significant impact on mechanical performance, particularly when the infill percentage is less than 100%. Structures with filament aligned at 0◦ and 100% infill showed the maximum elastic modulus, whereas specimens with filament oriented at 0◦ and 30% infill showed the lowest. The obtained numerical agreement indicated that an inverse method based only on the load–displacement curve can yield an accurate value for this material’s elastic modulus.National Innovation Agency (ANI) for MSc grant of Mariana Salgueiro nº POCI-01-0247- FEDER-039733 and Portuguese Foundations for Science and Technology. This project was co-financed by European Regional Development Fund (ERDF) through SI&IDT Projects in the framework of co-hosting—Competitiveness and Internationalisation Operational Programme (CIOP)—COMPETE 2020, Portugal 2020, with the National Innovation Agency (ANI) as the Intermediate Partner. Fabio Pereira acknowledges the Portuguese Foundation for Science and Technology, under the project UIDB/04033/2020. Mariana Salgueiro and Andrea Zille acknowledge the European Commission and the National Innovation Agency (ANI) for the financial support through the project “ARCHKNIT: Innovative smart textile interfaces for architectural applications”, Ref.: POCI-01-0247-FEDER-039733. This project was co-financed by European Regional Development Fund (ERDF) through SI&IDT Projects in the framework of co-hosting—Competitiveness and Internationalisation Operational Programme (CIOP)—COMPETE 2020, Portugal 2020, with the National Innovation Agency (ANI) as the Intermediate Partner. Nuno Dourado acknowledges FCT for the conceded financial support through the reference project UID/EEA/04436/2019 and “Programa bilateral de Portugal com a Tunísia”. Charii Fakher acknowledges the « Fondation pour la Recherche Scientifique” for the conceded financial support through “Programa bilateral de Portugal com a Tunísia”

Nutrient Content with Different Fertilizer Management and Influence on Yield and Fruit Quality in Apple cv. Gala

Assessing a plant’s nutritional status and fertilizer rates and types that can optimize fruit quality and yield are critical in intensive apple orchards. The aim of this work was to identify correlations between nutrients in the different organs that allow the early diagnosis of the nutritional status and to assess the impact on the optimal nutrient content in apple leaves, as well as in the yield and quality of chemical and organic fertilization. Five orchards of ‘Gala’ were fertilized with different levels of NPK over a period of four years. Macro and micronutrients of buds, flowers, 45 and 90–110 days after full bloom (DAFB) leaves and 60 DAFB and 15 days before harvest (DBH) fruits were determined. Boron was the only element for which strong correlations, 0.7 < r < 0.9, were observed between all organ pairs. The fertilization treatments did not affect the nutrient concentrations in the leaves of 90–110 DAFB other than P, Ca and Mg and did not affect the macronutrients in the fruit. In one of the five orchards, the yield increased by 26% with double fertilization compared to standard fertilization and, for the other four orchards, the impact depended on the year. Fruit size was more related to crop load than to fertilization and TSS and firmness were not affected by the type or amount of fertilizers. Replacing part of the chemical fertilizer with organic materials did not affect productivity or fruit qualityinfo:eu-repo/semantics/publishedVersio

Crenças face à violência conjugal: sexo, idade e região de residência

Poster apresentado no III Simpósio Olhares da Psicologia. 5 de Maio de 2016, Egas Moniz, Monte de Caparica, PortugalN/