Communication on plant health risks:Social science research, audience segmentation, and communication strategy for an EU awareness‐raising campaign

The European Food Safety Authority (EFSA) carried out preparatory work to inform an EU awareness-raising communication campaign on plant health risks. In a first phase, this included social science research and audience segmentation to inform appropriate choices regarding targeting and topics to be covered. Mixed methods research was carried out – including analysis of survey data, literature review and in-depth interviews –, to identify plant health awareness, knowledge, risk perception, preferred information sources and trust in different actors. Four “personas” were developed based on the results, representing four potential audience segments: “curious traveller” (P1), “home gardener and hobby farmer” (P2), “conscious young parent” (P3), and “adventurous and green foodie” (P4). The research also included an additional focus on stakeholders involved in plant health, agriculture, and related sectors since they can support the objectives and reach of a campaign. Then, in a second phase, the communication strategy for the 3-year campaign was designed, building on the social research as well as on situational analysis and a stakeholder mapping. The proposed strategy for the campaign foresees targeting of P1, P2 and P3, and entails using a progressive model aimed at raising target audiences’ awareness (Year 1), triggering critical thinking (Y2), and activating and creating advocacy (Y3). A crucial role will also be played by plant sector operators, who will be involved in dedicated activities and act as touchpoints and amplifiers for the campaign. The strategy provides a common framework allowing Member States (MSs) to fully localise the national implementation and to join the campaign throughout its 3-year course. The report outlines the objectives and topics defined for the different years of the campaign per target audience, as well as the tactics and the evaluation methodology. EFSA plans to launch the campaign in summer 2023 in collaboration with participating MSs

?-SiC NWs grown on patterned and MEMS silicon substrates

One-dimensional materials have become an attractive field of research due to their potential applications in physics, development of nanodevices and sensors. Nanowires (NW) are particularly interesting for their enhanced properties with respect to the bulk materials. Moreover the dimension of these nanostructures is comparable to those of the biological and chemical species being sensed. Silicon carbide in the form of NW is a material of great technological interest because of well known properties such as wide band gap, high temperature stability, extreme hardness, chemical inertness, biocompatibility. The deposition and the use of nano-patterned substrates as template in physics and biophysics is a first step to study adhesion of living cells and cell culture for biocompatibility and sensing. The fabrication of Micro Electrical Mechanics Systems (MEMS) with different geometries and patterned substrates for selective deposition can help to study the possibility to realize devices with higher performances. Here we present the growth of cubic silicon carbide nanowires on patterned silicon substrates and on silicon MEMS

European antibiotic awareness day : a five-year perspective of Europe-wide actions to promote prudent use of antibiotics

Following the European Union (EU) Council Recommendation on prudent use of antimicrobial agents in human medicine in 2001, and the success of national campaigns, i.e. Belgium and France, the European Centre for Disease Prevention and Control (ECDC) decided to establish the European Antibiotic Awareness Day (EAAD) on 18 November as platform to support national campaigns across Europe. This article provides an overview of EAAD tools, materials, and activities developed during the first five years. It shows that EAAD has been successful due to good cooperation between ECDC and national institutions, strong political and stakeholder support and evidence-based development of campaign materials. EAAD has provided a platform for pre-existing national campaigns and encouraged similar campaigns to develop where neither political support had been secured, nor financial support had been available. As a result, participating countries have continuously expressed strong support for ECDC to continue its work on EAAD. This has been endorsed by a steadily increasing number of countries participating and the growing interest of varied professional and stakeholder organisations. We conclude that EAAD should continue to act as catalyst for discussion and as mechanism to raise awareness of the public and prescribers about prudent use of antibiotics.peer-reviewe

The Hippo pathway effector TAZ induces intrahepatic cholangiocarcinoma in mice and is ubiquitously activated in the human disease

Background Intrahepatic cholangiocarcinoma (iCCA) is a highly aggressive primary liver tumor with increasing incidence worldwide, dismal prognosis, and few therapeutic options. Mounting evidence underlines the role of the Hippo pathway in this disease; however, the molecular mechanisms whereby the Hippo cascade contributes to cholangiocarcinogenesis remain poorly defined. Methods We established novel iCCA mouse models via hydrodynamic transfection of an activated form of transcriptional coactivator with PDZ-binding motif (TAZ), a Hippo pathway downstream effector, either alone or combined with the myristoylated AKT (myr-AKT) protooncogene, in the mouse liver. Hematoxylin and eosin staining, immunohistochemistry, electron microscopy, and quantitative real-time RT-PCR were applied to characterize the models. In addition, in vitro cell line studies were conducted to address the growth-promoting roles of TAZ and its paralog YAP. Results Overexpression of TAZ in the mouse liver triggered iCCA development with very low incidence and long latency. In contrast, co-expression of TAZ and myr-AKT dramatically increased tumor frequency and accelerated cancer formation in mice, with 100% iCCA incidence and high tumor burden by 10 weeks post hydrodynamic injection. AKT/TAZ tumors faithfully recapitulated many of the histomolecular features of human iCCA. At the molecular level, the development of the cholangiocellular lesions depended on the binding of TAZ to TEAD transcription factors. In addition, inhibition of the Notch pathway did not hamper carcinogenesis but suppressed the cholangiocellular phenotype of AKT/TAZ tumors. Also, knockdown of YAP, the TAZ paralog, delayed cholangiocarcinogenesis in AKT/TAZ mice without affecting the tumor phenotype. Furthermore, human preinvasive and invasive iCCAs and mixed hepatocellular carcinoma/iCCA displayed widespread TAZ activation and downregulation of the mechanisms protecting TAZ from proteolysis. Conclusions Overall, the present data underscore the crucial role of TAZ in cholangiocarcinogenesi

Lopinavir/Ritonavir and Darunavir/Cobicistat in Hospitalized COVID-19 Patients: Findings From the Multicenter Italian CORIST Study

Background: Protease inhibitors have been considered as possible therapeutic agents for COVID-19 patients. Objectives: To describe the association between lopinavir/ritonavir (LPV/r) or darunavir/cobicistat (DRV/c) use and in-hospital mortality in COVID-19 patients. Study Design: Multicenter observational study of COVID-19 patients admitted in 33 Italian hospitals. Medications, preexisting conditions, clinical measures, and outcomes were extracted from medical records. Patients were retrospectively divided in three groups, according to use of LPV/r, DRV/c or none of them. Primary outcome in a time-to event analysis was death. We used Cox proportional-hazards models with inverse probability of treatment weighting by multinomial propensity scores. Results: Out of 3,451 patients, 33.3% LPV/r and 13.9% received DRV/c. Patients receiving LPV/r or DRV/c were more likely younger, men, had higher C-reactive protein levels while less likely had hypertension, cardiovascular, pulmonary or kidney disease. After adjustment for propensity scores, LPV/r use was not associated with mortality (HR = 0.94, 95% CI 0.78 to 1.13), whereas treatment with DRV/c was associated with a higher death risk (HR = 1.89, 1.53 to 2.34, E-value = 2.43). This increased risk was more marked in women, in elderly, in patients with higher severity of COVID-19 and in patients receiving other COVID-19 drugs. Conclusions: In a large cohort of Italian patients hospitalized for COVID-19 in a real-life setting, the use of LPV/r treatment did not change death rate, while DRV/c was associated with increased mortality. Within the limits of an observational study, these data do not support the use of LPV/r or DRV/c in COVID-19 patients

Cosmic ray physics with the ARGO-YBJ experiment

The main scientific goals of the ARGO-YBJ experiment are ray astronomy with a few hundreds GeV energy threshold and cosmic ray physics below and around the knee of the primary energy spectrum (10**12−10**16 eV), where the transition from direct to indirect measurement techniques takes place. The ARGO-YBJ experiment, located at the Cosmic Ray Observatory of Yangbajing (Tibet, P.R. of China, 4 300 m a.s.l.), is an unconventional Extensive Air Shower array of about 6,700 m2 of active area, the only one exploiting the full-coverage technique at very high altitude currently in operation. The detector space-time granularity, performance and location offer a unique chance to make a detailed study of the structure of cosmic ray showers, in particular of the hadronic component. In this work we will focus on the main experimental results concerning cosmic ray and hadronic interaction physics: primary cosmic ray energy spectrum, antiproton over proton ratio, anisotropy in the cosmic ray flux and proton-air cross-section. Moreover, the possible data analysis improvements based on the use of all detailed information on the shower front (curvature, time width, rise time and so on), as well as the extension of the investigable energy range, allowed by the analog RPC readout, will be pointed out

First results on the angular resolution of the ARGO-YBJ detector

We present the first results on the angular resolution of the ARGO-YBJ detector in data taking at the Yangbajing Laboratory (Tibet, P.R. China, 4300 m a.s.l.

Gamma-Ray Astronomy with ARGO-YBJ

ARGO-YBJ is a full coverage air shower array located at the YangBaJing Cosmic Ray Laboratory (Tibet, P.R. China, 4300 m a.s.l., 606 g/cm2) recording data with a duty cycle 85% and an energy threshold of a few hundred GeV. In this paper the latest results in Gamma-Ray Astronomy are summarized

Simulation study of air shower particles near the core region

The ARGO-YBJ experiment has two kinds of signals in the shower working mode which allows coverage of the energy region from TeV to PeV region. One is the digital strip pattern, another is so-called ‘big pad’ mode, which is the analog signal counting the pulse height on half of an RPC, proportional to the number of hitting particles. In this paper by using the Monte Carlo simulation method the ARGO-YBJ sensitivity to the cosmic ray composition is discussed, by using the ‘big pad’ signal for measuring the number of particles detected close to the shower core

Search for gamma ray bursts with the ARGO-YBJ detector in scaler mode

The ARGO-YBJ experiment has been designed to decrease the energy threshold of typical Extensive Air Shower arrays by exploiting the high altitude and the full coverage, consisting of a 6700m2 carpet of Resistive Plate Chambers located at Yangbajing (Tibet, PR China, 4300m a.s.l.). The lower energy limit of the detector (1 GeV) is reached with the ‘‘Scaler Mode’’, recording the counting rate at fixed time intervals. Here we present results concerning the search for emission from Gamma Ray Bursts (GRBs) in coincidence with satellite detections