National Outbreak of Salmonella Serotype Saintpaul Infections: Importance of Texas Restaurant Investigations in Implicating Jalapeño Peppers

BACKGROUND: In May 2008, PulseNet detected a multistate outbreak of Salmonella enterica serotype Saintpaul infections. Initial investigations identified an epidemiologic association between illness and consumption of raw tomatoes, yet cases continued. In mid-June, we investigated two clusters of outbreak strain infections in Texas among patrons of Restaurant A and two establishments of Restaurant Chain B to determine the outbreak's source. METHODOLOGY/PRINCIPAL FINDINGS: We conducted independent case-control studies of Restaurant A and B patrons. Patients were matched to well controls by meal date. We conducted restaurant environmental investigations and traced the origin of implicated products. Forty-seven case-patients and 40 controls were enrolled in the Restaurant A study. Thirty case-patients and 31 controls were enrolled in the Restaurant Chain B study. In both studies, illness was independently associated with only one menu item, fresh salsa (Restaurant A: matched odds ratio [mOR], 37; 95% confidence interval [CI], 7.2-386; Restaurant B: mOR, 13; 95% CI 1.3-infinity). The only ingredient in common between the two salsas was raw jalapeño peppers. Cultures of jalapeño peppers collected from an importer that supplied Restaurant Chain B and serrano peppers and irrigation water from a Mexican farm that supplied that importer with jalapeño and serrano peppers grew the outbreak strain. CONCLUSIONS/SIGNIFICANCE: Jalapeño peppers, contaminated before arrival at the restaurants and served in uncooked fresh salsas, were the source of these infections. Our investigations, critical in understanding the broader multistate outbreak, exemplify an effective approach to investigating large foodborne outbreaks. Additional measures are needed to reduce produce contamination

Antiviral Peptides as Promising Therapeutics against SARS-CoV-2

Over 50 peptides, which were known to inhibit SARS-CoV-1, were computationally screened against the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2. Based on the binding affinity and interaction, 15 peptides were selected, which showed higher affinity compared to the α-helix of the human ACE2 receptor. Molecular dynamics simulation demonstrated that two peptides, S2P25 and S2P26, were the most promising candidates, which could potentially block the entry of SARS-CoV-2. Tyr489 and Tyr505 residues present in the "finger-like" projections of the RBD were found to be critical for peptide interaction. Hydrogen bonding and hydrophobic interactions played important roles in prompting peptide-protein binding and interaction. Structure-activity relationship indicated that peptides containing aromatic (Tyr and Phe), nonpolar (Pro, Gly, Leu, and Ala), and polar (Asn, Gln, and Cys) residues were the most significant contributors. These findings can facilitate the rational design of selective peptide inhibitors targeting the spike protein of SARS-CoV-2

NMDA receptor antagonists: Repositioning of memantine as a multitargeting agent for Alzheimer\u27s therapy

Alzheimer&rsquo;s disease (AD) is a progressive neurodegenerative disease that causes problems with memory,thinking, and behavior. Currently, there is no drug that can reduce the pathological events of this degenerativedisease but symptomatic relief is possible that can abate the disease condition. N-methyl-D-aspartate(NMDA) receptors exert a critical role for synaptic plasticity as well as transmission. Overstimulation of glutamatereceptors, predominantly NMDA type, may cause excitotoxic effects on neurons and is recommended as amechanism for neurodegeneration. Atypical activation of the NMDA receptor has been suggested for AD bysynaptic dysfunction. NMDA receptor antagonists especially memantine block the NMDA receptor and can reducethe influx of calcium (Ca2+) ions into neuron, thus, toxic intracellular events are not activated. This reviewrepresents the role of NMDA receptors antagonists as potential therapeutic agents to reduce AD. Moreover, thisreview highlights the repositioning of memantine as a potential novel therapeutic multitargeting agent for AD.</jats:sec