Differential proteomic analysis of Acidithiobacillus ferrooxidans cells maintained in contact with bornite or chalcopyrite: Proteins involved with the early bacterial response

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Acidithiobacillus ferrooxidans is a chemoautotrophic bacterium capable of oxidizing ferrous iron or sulfides to obtain energy. Bornite and chalcopyrite are copper sulfides containing iron that present different susceptibilities to the bioleaching process. Here, the early bacterial response to these minerals was investigated using a differential proteomic approach. The protein profiles of cells kept in contact with bornite or chalcopyrite for 24 or 48 h were compared to that of cells not exposed to the minerals. Response to bornite exposure involved thirteen proteins, mainly related to energy metabolism, detoxification and protein synthesis. We detected increases in the expression levels of the proteins chaperonin, antioxidant and aldehyde dehydrogenase, as well as decreases in the expression levels of the proteins radical SAM domain, fructose-1,6-bisphosphatase, PfkB domain, heat shock HslVU. ribulose bisphosphate carboxylase and ribosomal proteins. Chalcopyrite contact led to a distinct metabolic response of the bacterium, since no significant alteration in the level of protein expression was detected. These findings could help to understand the metabolic impact in A. ferrooxidans after the initial addition of the cells to bornite or chalcopyrite during bioleaching processes. (C) 2010 Elsevier Ltd. All rights reserved.463770776Vale (Brazil)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)ProteoRed for the proteomics networkConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Psychosocial care in dementia in European higher education: Evidence from the SiDECar (“Skills in DEmentia Care”) project

none12siIn dementia care, psychosocial interventions can increase people's quality of life with dementia and their caregivers. Despite their effectiveness, their translation into practice lacks the desirable systematicity. Systematic educational programs on psychosocial interventions in dementia will improve this translation, as it prepares professionals to face the complexity of dementia care. This study aimed to systematically map out the extent to which higher education programs in Europe include teaching activities about psychosocial care of dementia. We collected quantitative and qualitative data about 303 higher education teaching activities on psychosocial care in dementia across Europe. The analysis revealed that the number of teaching activities focusing on psychosocial care in dementia was relative. Although the results reflected UNESCO indications, the teaching activities on psychosocial care in dementia appeared less systematized than optimal. As world health agencies recommend, international higher education systems should consider more psychosocial care topics because they can prepare professionals to respond timely and effectively to dementia patients and caregivers' needs.embargoed_20210228Ottoboni, G.; Chirico, I.; Povolná, P.; Dostálová, V.; Holmerová, I.; Janssen, N.; Dassen, F.; de Vugt, M.; Sánchez-Gómez, Ma.C.; García-Peñalvo, F.; Franco-Martin, M.A.; Chattat, R.Ottoboni, G.; Chirico, I.; Povolná, P.; Dostálová, V.; Holmerová, I.; Janssen, N.; Dassen, F.; de Vugt, M.; Sánchez-Gómez, Ma.C.; García-Peñalvo, F.; Franco-Martin, M.A.; Chattat, R