Enanismo hipofisario en un canino Poodle: hallazgos clínicos y de laboratorio

This paper aimed to evaluate the clinical and laboratory findings of a case of pituitary dwarfism in a 4-month-old female Poodle dog. The clinical signs observed were a reduced body size, soft and woolly coat, retention of the sub-coat, absence of primary hairs, scaling of the skin of the head region and deciduous teeth. Hormonal analyses indicated values of 0.5 ng/ml for growth hormone (GH), 0.44 ng/ml for total T3 and 1.44 ng/dl for free T4. GH levels were below normal, thus indicating a pituitary dwarfism.El presente trabajo tuvo como objetivo evaluar los hallazgos clínicos y de laboratorio de un caso de enanismo hipofisario en un canino de raza Poodle, hembra de 4 meses. Los signos clínicos observados fueron un menor tamaño corporal, pelaje suave y lanoso, retención del sub-pelaje, ausencia de pelos primarios, descamación de piel de la región de la cabeza y dientes de leche. Los análisis hormonales indicaron valores de 0.5 ng/ml para la hormona del crecimiento (GH), 0.44 ng/ml para T3 total y 1.44 ng/dl para T4 libre. Los niveles de GH estaban debajo de lo normal, indicando así, un cuadro de enanismo hipofisario

Functional and morphological assessment of a standardized rat sciatic nerve crush injury with a non-serrated clamp

Peripheral nerve researchers frequently use the rat sciatic nerve crush as a model for axonotmesis.Unfortunately, studies from various research groups report results from different crush techniquesand by using a variety of evaluation tools, making comparisons between studies difficult. The pur-pose of this investigation was to determine the sequence of functional and morphologic changes af-ter an acute sciatic nerve crush injury with a non-serrated clamp, giving a final standardized pres-sure of p9 MPa. Functional recovery was evaluated using the sciatic functional index (SFI), theextensor postural thrust (EPT) and the withdrawal reflex latency (WRL), before injury, and thenat weekly intervals until week 8 postoperatively. The rats were also evaluated preoperatively andat weeks 2, 4, and 8 by ankle kinematics, toe out angle (TOA), and gait-stance duration. In addi-tion, the motor nerve conduction velocity (MNCV) and the gastrocnemius-soleus weight parameterswere measured just before euthanasia. Finally, structural, ultrastructural and histomorphometricanalyses were carried out on regenerated nerve fibers. At 8 weeks after the crush injury, a full func-tional recovery was predicted by SFI, EPT, TOA, and gait-stance duration, while all the other pa-rameters were still recovering their original values. On the other hand, only two of the histomor-phometric parameters of regenerated nerve fibers, namely myelin thickness/axon diameter ratio andfiber/axon diameter ratio, returned to normal values while all other parameters were significantlydifferent from normal values. The employment of traditional methods of functional evaluation inconjunction with the modern techniques of computerized analysis of gait and histomorphometricanalysis should thus be recommended for an overall assessment of recovery in the rat sciatic nervecrush model

Supramolecular organizations in the aerobic respiratory chain of Escherichia coli

The organization of respiratory chain complexes in supercomplexes has been shown in the mitochondria of several eukaryotes and in the cell membranes of some bacteria. These supercomplexes are suggested to be important for oxidative phosphorylation efficiency and to prevent the formation of reactive oxygen species. Here we describe, for the first time, the identification of supramolecular organizations in the aerobic respiratory chain of Escherichia coli, including a trimer of succinate dehydrogenase. Furthermore, two heterooligomerizations have been shown: one resulting from the association of the NADH:quinone oxidoreductases NDH-1 and NDH-2, and another composed by the cytochrome bo3 quinol:oxygen reductase, cytochrome bd quinol:oxygen reductase and formate dehydrogenase (fdo). These results are supported by blue native-electrophoresis, mass spectrometry and kinetic data of wild type and mutant E . coli strains

Draft genome sequence of the N2-fixing cyanobacterium Nostoc piscinale CENA21, isolated from the Brazilian Amazon floodplain

We announce here the draft genome sequence of Nostoc piscinale CENA21, a diazotrophic heterocyst-forming cyanobacterium isolated from the Solimões River, Amazon Basin, Brazil. It consists of one circular chromosome scaffold with 11 contigs and total size of 7,094,556 bp. Secondary metabolite annotations indicate a good source for the discovery of novel natural products. © 2016 Leão et al.This work has been supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and from the Funda ção de Amparo à Pesquisa do Estado do Pará (BIONORTE-CNPq/FAPESPA) to M.P.C.S. This work, including the efforts of Marli Fatima Fiore and Maria Paula Cruz Schneider, was funded by MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (307045/2010-2 and 306607/2012-3). This work, including the efforts of Tiago Leão and Pedro Ivo Guimaraes, was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (13425-13-7 and 13194-13-5). This work, including the efforts of Pedro Nuno Leão, was funded by Ministry of Education and Science | Fundação para a Ciência e a Tecnologia (FCT) (IF/01358/2014)

Common Proper Motion Search for Faint Companions Around Early-Type Field Stars - Progress Report

The multiplicity of early-type stars is still not well established. The derived binary fraction is different for individual star forming regions, suggesting a connection with the age and the environment conditions. The few studies that have investigated this connection do not provide conclusive results. To fill in this gap, we started the first detailed adaptive-optic-assisted imaging survey of early-type field stars to derive their multiplicity in a homogeneous way. The sample has been extracted from the Hipparcos Catalog and consists of 341 BA-type stars within ~300 pc from the Sun. We report the current status of the survey and describe a Monte-Carlo simulation that estimates the completeness of our companion detection.Comment: 4 pages, 1 figure, conference proc. Syros 200