Structural Activity Relationship Study on Dual PLK1 /BRD4 Inhibitor, BI- 2536

Polo-like kinase 1 (PLK1) and BRD4 are two different therapeutic targets in cancer drug discovery. Recently it has been reported that PLK1 inhibitor, BI-2536, is also a potent inhibitor of BRD4. The simultaneous inhibition of PLK1 and BRD4 by a single drug molecule is interesting because this could lead to the development of effective therapeutic strategy for different types of disease conditions in which PLK1 and BRD4 are implicated. Structural activity relationship studies has been carried out on BI-2536 to generate analogs with enhanced dual inhibitory activity against BRD4 and PLK1 as well as to render the molecule selective to one target over the other. UMB101 and 160 have been found to exhibit enhanced dual inhibitory activity with selectivity fold of less than 30, UMB160 being the most potent dual-kinase bromodomain inhibitor (BRD4 IC50 = 28 nM, PLK1 IC50 = 40 nM). UMB131 was found to be the most selective PLK1 inhibitor over BRD4

The study of production Artemia enrichment liquid SELCO1 &SUPER2 SELCO with internal capacities

Artemia as a live food has multiple applications in aquaculture. Artemia contains few unsaturated 3-omega fatty acids particularly eicozapentanoeic acid (EPA) and has no 6-omega fatty acids particularly decozahexanoeic acid (DHA), so Artemia naplious is enriched to improve its food values. The most famous enrichment emulsion are selco and super-selco made by Euro-American INVE Company. This study was performed to make Artemia enrichment emulsions by internal potentials. At first, the final composition of Artemia enrichment emulsion (selco) was determined in Urmia university chemical analysis laboratory. Then, aquatic fishing resources in south of the country such as eye oil of tuna fish, shark liver, cuttlefish and plant oils of sunflower, olive and beef oil were used. Fatty acids profiles were analyzed by Gas Chromatography (GC). The results showed that cuttlefish may produce 13± 3 % wet weight of fatty acids. We made 3 enrichment oils which contained 60± 10 % of plant and animal oils. These suspensions fatty acids were analyzed and the results were compared with control sample. Field tests were performed on 200000 Artemia urmiana naplious by enriching the Artemia with enrichment emulsions and the products were analyzed by GC. The results indicated that the rate of emulsions absorbance in imported and internal samples were 37.47, 25.30, 18.88, 22.14 and 10.32 respectively. In the next stage, enriched Artemia naplious were fed as live food to 500 trout larvae in Ziveh Aquaculture Company as follows: Treatment 1- Control consisted of new feeding larvae fed by concentrated food Treatment 2- new feeding larvae fed by concentrated food plus un-enriched naplious Treatment 3- new feeding larvae fed by concentrated food plus selco oil enriched naplious Treatment 4- new feeding larvae fed by concentrated food plus emulsion-1 oil enriched naplious Treatment 5- new feeding larvae fed by concentrated food plus emulsion-2 oil enriched naplious Treatment 6- new feeding larvae fed by concentrated food plus emulsion-3 oil enriched naplious The results indicated that treatments 1, 2 had significant difference with treatments 3, 4, 5, 6 from survival rate, growth coefficient, obesity coefficient, total length, food conversion coefficient, final weight and protein percent. Abnormalities rates in treatments 1, 2 had significant difference with treatments 3, 4, 5 , 6 in which enriched emulsions were not used, but these indices had no significant difference with commercial samples which shows internal made emulsions can easily be used. The data were analyzed by one-way analyses variance and Duncan test in SPSS and EXCELL softwares. In conclusion, we can make enrichment selco oils in the country by internal potentials which the foreign samples can be replaced by them

Prevalence of brucellosis and associated risk factors in dairy cattle in Maekel and Debub Regions, Eritrea

IntroductionBrucellosis is a zoonotic disease with worldwide distribution. It is considered endemic in Eritrea, however, the current prevalence status and related risk factors in animals are unknown. The objective of this study was to determine the prevalence of and risk factors for brucellosis in dairy cattle in Maekel and Debub regions, Eritrea.MethodsA cross sectional study was conducted between August 2021 and February 2022. A total of 2,740 dairy cattle from 214 herds in 10 sub-regions of Eritrea were selected for blood and data collection. Blood samples were tested using Rose Bengal Plate Test (RBPT) and positive samples were confirmed using competitive (c-ELISA). Data on risk factors was collected using questionnaire and analyzed using logistic regression.ResultsIn total, 34/2740 animals tested positive by RBPT. Of these, 29 were confirmed positive by c-ELISA, giving an apparent and estimated true individual-level prevalence of 1.1% (95% CI: 0.7, 1.5%) and 1.3% (95% CI: 0.9, 1.8%), respectively. Sixteen herds (7.5%) tested positive by RBPT and of these 15 herds (7.0%) were confirmed positive by c-ELISA, giving an estimated true herd-level prevalence of 7.0% (95% CI: 4.0, 10.7). Animal and herd-level apparent prevalence was 1.6 and 9.2% in Maekel, while in Debub it was 0.6 and 5.5%, respectively. Multivariable regression analysis indicated that non-pregnant lactating cows (adjusted odds ratio [aOR] = 3.35; p = 0.042) were more likely to be Brucella sero-positive. History of abortion on the farm (aOR = 5.71; p = 0.026) and larger number of cows in the herd (aOR = 1.14; p &amp;lt; 0.001) were associated with brucellosis sero-positivity in herds.ConclusionBrucellosis prevalence was low in the study areas. Nonetheless, this low prevalence may increase if the disease is not controlled. Therefore, testing animals before movement, good farming practices, sanitary measures, and an awareness raising program on brucellosis are recommended.</jats:sec

Surgical treatment of neglected congenital idiopathic talipes equinovarus after walking age in Eritrea: an Italo-Eritrean cooperation

An Italian team of orthopaedic surgeons joined Eritrean colleagues to perform a clinical study in ambulating children affected by neglected idiopathic congenital talipes equinovarus (clubfoot). This study reports the surgical strategy as well as clinical outcomes, early complications and relapse at a mid-term follow-up. Four expeditions of 7 days were organized between 2012 and 2015 from Italy to the Halibet Hospital of Asmara in Eritrea. In each expedition were included two experienced surgeons, two assistants and one anaesthesiologist. During these expeditions, a total of 468 patients were evaluated together with Eritrean colleagues and 45 cases of neglected talipes equinovarus in ambulating children were diagnosed and selected for surgery. Follow-up range was 1–3 years. During the four expeditions, the Eritrean team of orthopaedic surgeons learned to manage most cases of neglected talipes equinovarus. No major complications were reported. Sixteen feet were considered excellent, 25 good and four poor. No overcorrections were observed. Neglected congenital talipes equinovarus is the result of delayed treatment of congenital deformity in developing countries, and its treatment often requires extensive surgery. Collaboration with foreign expert surgeons may help local doctors to learn how to treat this disease. The current study demonstrates that surgical expeditions in developing countries, when organized in collaboration with local doctors, help to manage on site this severe deformity

The 1 Ma old human assemblage from the Homo site at Uadi Aalad, Buia (Danakil Depression of Eritrea): an updated record

International audienc

The late Early Pleistocene human dental remains from Uadi Aalad and Mulhuli-Amo (Buia), Eritrean Danakil: macromorphology and microstructure

Fieldwork performed during the last 15 years in various Early Pleistocene East African sites has significantly enlarged the fossil record of Homo erectus sensu lato (s.l.). Additional evidence comes from the Danakil Depression of Eritrea, where over 200 late Early to early Middle Pleistocene sites have been identified within a ∼1000 m-thick sedimentary succession outcropping in the Dandiero Rift Basin, near Buia. Along with an adult cranium (UA 31), which displays a blend of H. erectus-like and derived morpho-architectural features and three pelvic remains, two isolated permanent incisors (UA 222 and UA 369) have also been recovered from the 1 Ma (millions of years ago) Homo-bearing outcrop of Uadi Aalad. Since 2010, our surveys have expanded to the nearby (4.7 km) site of Mulhuli-Amo (MA). This is a fossiliferous area that has been preliminarily surveyed because of its exceptional concentration of Acheulean stone tools. So far, the site has yielded 10 human remains, including the unworn crown of a lower permanent molar (MA 93). Using diverse analytical tools (including high resolution μCT and μMRI), we analysed the external and internal macromorphology and microstructure of the three specimens, and whenever possible compared the results with similar evidence from early Homo, H. erectus s.l., H. antecessor, H. heidelbergensis (from North Africa), Neanderthals and modern humans. We also assessed the UA 369 lower incisor from Uadi Aalad for root completion timing and showed that it compares well with data for root apex closure in modern human populations

Surgical treatment of neglected congenital idiopathic talipes equinovarus after walking age in Eritrea: an Italo-Eritrean cooperation

An Italian team of orthopaedic surgeons joined Eritrean colleagues to perform a clinical study in ambulating children affected by neglected idiopathic congenital talipes equinovarus (clubfoot). This study reports the surgical strategy as well as clinical outcomes, early complications and relapse at a mid-term follow-up. Four expeditions of 7 days were organized between 2012 and 2015 from Italy to the Halibet Hospital of Asmara in Eritrea. In each expedition were included two experienced surgeons, two assistants and one anaesthesiologist. During these expeditions, a total of 468 patients were evaluated together with Eritrean colleagues and 45 cases of neglected talipes equinovarus in ambulating children were diagnosed and selected for surgery. Follow-up range was 1–3 years. During the four expeditions, the Eritrean team of orthopaedic surgeons learned to manage most cases of neglected talipes equinovarus. No major complications were reported. Sixteen feet were considered excellent, 25 good and four poor. No overcorrections were observed. Neglected congenital talipes equinovarus is the result of delayed treatment of congenital deformity in developing countries, and its treatment often requires extensive surgery. Collaboration with foreign expert surgeons may help local doctors to learn how to treat this disease. The current study demonstrates that surgical expeditions in developing countries, when organized in collaboration with local doctors, help to manage on site this severe deformity