Patients with Duchenne and Becker muscular dystrophies are not more asymmetrical than healthy controls on timed performance of upper limb tasks

This study aimed to investigate possible asymmetries and relationships between performance of dominant and non-dominant upper limbs (UL) in patients with Duchenne and Becker muscular dystrophies (DMD/BMD), to compare UL performance of patients and healthy subjects and to investigate the relationship between timed performance of UL and age, motor function and muscle strength in DMD/BMD patients. Sixteen patients with DMD and 3 with BMD were evaluated with Jebsen-Taylor Test (timed performance), Vignos scale and Dimension 3 of Motor Function Measure (motor function), and Medical Research Council scale (muscle strength) on a single session. ANOVA showed no asymmetry between dominant and non-dominant UL, except in the writing subtest, in patients and in healthy controls. There were relationships between dominant and non-dominant UL performances. Correlations between timed performance, motor function and muscle strength were found, but age was not correlated with these variables. These findings may reduce the assessment time, prevent fatigue and provide more accurate clinical reasoning involving UL in DMD/BMD treatment.Univ Sao Paulo, Fac Med, Dept Fonoaudiol Fisioterapia & Terapia Ocupac, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Ciencias Movimento Humano, Santos, SP, BrazilUniv Fed Sao Paulo, Dept Neurol Clin, Dept Neurol Neurocirurgia, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Ciencias Movimento Humano, Santos, SP, BrazilUniv Fed Sao Paulo, Dept Neurol Clin, Dept Neurol Neurocirurgia, Sao Paulo, SP, BrazilWeb of Scienc

Relationship between muscle strength and motor function in Duchenne muscular dystrophy

Measuring muscle strength and motor function is part of Duchenne muscular dystrophy (DMD) assessment. However, the relationship between these variables is controversial. Objective: To investigate the relationship between muscle strength and motor function and between these variables and age. Method: Muscle strength was measured by Medical Research Council (MRC) scale and motor function, by Motor Function Measure (MFM), in 40 non-ambulatory patients. Spearman tests investigated the relationships between muscle strength, motor function and age. Results: Total MRC and MFM scores were strongly related to each other (r = 0.94p 0.05). Strong and moderate relationships between partial muscle strength and motor function scores were found. Higher correlation coefficients were found between total scores and Dimensions 2 (axial/ proximal control) and 3 (distal control) of MFM. Conclusion: Muscle strength and motor function are strongly correlated and seem to decrease proportionally in DMD.Univ Sao Paulo, Fac Med, Dept Fisioterapia Fonoaudiol & Terapia Ocupac, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Fac Med, Dept Neurol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Fac Med, Dept Neurol, Sao Paulo, SP, BrazilWeb of Scienc

Towards an autonomous vision-based unmanned aerial system againstwildlife poachers

Poaching is an illegal activity that remains out of control in many countries. Based on the 2014 report of the United Nations and Interpol, the illegal trade of global wildlife and natural resources amounts to nearly $213 billion every year, which is even helping to fund armed conflicts. Poaching activities around the world are further pushing many animal species on the brink of extinction. Unfortunately, the traditional methods to fight against poachers are not enough, hence the new demands for more efficient approaches. In this context, the use of new technologies on sensors and algorithms, as well as aerial platforms is crucial to face the high increase of poaching activities in the last few years. Our work is focused on the use of vision sensors on UAVs for the detection and tracking of animals and poachers, as well as the use of such sensors to control quadrotors during autonomous vehicle following and autonomous landing.Peer Reviewe

Investigation Of The Shape Of A Cloud Of Electron-hole Droplets In Germanium

Absorption and scattering of light are used to investigate a cloud of electron-hole drops in Ge at 2°K. We find a drop size of 2 m, from light scattering; and use an Abel transform to unfold the absorption data and obtain a complete droplet density map. The cloud is distinctly nonspherical. The droplet penetration in a direction perpendicular to the surface of excitation is 1.5 times greater than that in a parallel direction. This result is discussed in terms of alternative models for cloud formation. © 1976 The American Physical Society.13125603560

Autonomous Optimal Absolute Orbit Keeping through Formation Flying Techniques

peer reviewedIn this paper, the problem of autonomous optimal absolute orbit keeping for a satellite mission in Low Earth Orbit using electric propulsion is considered. The main peculiarity of the approach is to support small satellite missions in which the platform is equipped with a single thruster nozzle that provides acceleration on a single direction at a time. This constraint implies that an attitude maneuver is necessary before or during each thrusting arc to direct the nozzle into the desired direction. In this context, an attitude guidance algorithm specific for the orbit maneuver has been developed. A Model Predictive Control scheme is proposed, where the attitude kinematics are coupled with the orbital dynamics in order to obtain the optimal guidance profiles in terms of satellite state, reference attitude, and thrust magnitude. The proposed control scheme is developed exploiting formation flying techniques where the reference orbit is that of a virtual spacecraft that the main satellite is required to rendezvous with. In addition to the controller design, the closed-loop configuration is presented supported by numerical simulations. The efficacy of the proposed autonomous orbit-keeping approach is shown in several application scenarios.Autonomous Constellation and Formation Control of Microsatellite

Refractive elastic scattering of carbon and oxygen nuclei: The mean field analysis and Airy structures

The experimental data on the $^{16}$O$+^{12}$C and $^{18}$O$+^{12}$C elastic scatterings and their optical model analysis are presented. Detailed and complete elastic angular distributions have been measured at the Strasbourg Vivitron accelerator at several energies covering the energy range between 5 and 10 MeV per nucleon. The elastic scattering angular distributions show the usual diffraction pattern and also, at larger angles, refractive effects in the form of nuclear rainbow and associated Airy structures. The optical model analysis unambiguously shows the evolution of the refractive scattering pattern. The observed structure, namely the Airy minima, can be consistently described by a nucleus-nucleus potential with a deep real part and a weakly absorptive imaginary part. The difference in absorption in the two systems is explained by an increased imaginary (mostly surface) part of the potential in the $^{18}$O$+^{12}$C system. The relation between the obtained potentials and those reported for the symmetrical $^{16}$O$+^{16}$O and $^{12}$C$+^{12}$C systems is drawn.Comment: 10 pages, 9 figures, Phys. rev. C in pres

Hsp70 in mitochondrial biogenesis

The family of hsp70 (70 kilodalton heat shock protein) molecular chaperones plays an essential and diverse role in cellular physiology, Hsp70 proteins appear to elicit their effects by interacting with polypeptides that present domains which exhibit non-native conformations at distinct stages during their life in the cell. In this paper we review work pertaining to the functions of hsp70 proteins in chaperoning mitochondrial protein biogenesis. Hsp70 proteins function in protein synthesis, protein translocation across mitochondrial membranes, protein folding and finally the delivery of misfolded proteins to proteolytic enzymes in the mitochondrial matrix

Plasma kallikrein structure reveals apple domain disc rotated conformation compared to factor XI

BackgroundPlasma prekallikrein (PK) and factor XI (FXI) are apple domain‐containing serine proteases that when activated to PKa and FXIa cleave substrates kininogen, factor XII, and factor IX, respectively, directing plasma coagulation, bradykinin release, inflammation, and thrombosis pathways.ObjectiveTo investigate the three‐dimensional structure of full‐length PKa and perform a comparison with FXI.MethodsA series of recombinant full‐length PKa and FXI constructs and variants were developed and the crystal structures determined.Results and conclusionsA 1.3 Å structure of full‐length PKa reveals the protease domain positioned above a disc‐shaped assemblage of four apple domains in an active conformation. A comparison with the homologous FXI structure reveals the intramolecular disulfide and structural differences in the apple 4 domain that prevents dimer formation in PK as opposed to FXI. Two latchlike loops (LL1 and LL2) extend from the PKa protease domain to form interactions with the apple 1 and apple 3 domains, respectively. A major unexpected difference in the PKa structure compared to FXI is the 180° disc rotation of the apple domains relative to the protease domain. This results in a switched configuration of the latch loops such that LL2 interacts and buries portions of the apple 3 domain in the FXI zymogen whereas in PKa LL2 interacts with the apple 1 domain. Hydrogen‐deuterium exchange mass spectrometry on plasma purified human PK and PKa determined that regions of the apple 3 domain have increased surface exposure in PKa compared to the zymogen PK, suggesting conformational change upon activation

Expression of vascular endothelial growth factor mRNA in non-small-cell lung carcinomas

The vascular endothelial growth factor (VEGF) has been shown to be strictly related to vascular permeability and endothelial cell growth under physiological and pathological conditions. In tumour development and progression, VEGF plays a pivotal role in the development of the tumoral vascular network, and useful information in the progression of human cancer can be obtained by analysing the vascular endothelial growth factor expression of the tumours. In this study, we investigated the vascular endothelial growth factor transcript expression in non-small-cell lung carcinomas to evaluate the significance of this factor in a group of cancers in which the vascular pattern has been shown to significantly affect progression. Surgical samples of 42 patients with NSCLC were studied using reverse transcription polymerase chain reaction (PCR) analysis and in situ hybridization. Thirty-three out of 42 cases (78.6%) showed VEGF transcript expression predominantly as transcripts for the secretory forms of VEGF (isoforms 121 and 165). In situ hybridization, performed on 24 out of 42 samples, showed that the VEGF transcript expression was in several cases present in the cytoplasm both of neoplastic and normal cells, even if the VEGF mRNA was less expressed in the corresponding non-tumoral part. The VEGF 121 expression was associated with hilar and/or mediastinal nodal involvement (P = 0.02), and, taken together, the VEGF isoforms were shown to significantly influence overall (P = 0.02) and disease-free survival (P = 0.03). As a regulator of tumour angiogenesis, VEGF may represent a useful indicator of progression and poor prognosis in non-small-cell lung carcinomas. © 1999 Cancer Research Campaig

Histoplasma capsulatum Heat-Shock 60 Orchestrates the Adaptation of the Fungus to Temperature Stress

Heat shock proteins (Hsps) are among the most widely distributed and evolutionary conserved proteins. Hsps are essential regulators of diverse constitutive metabolic processes and are markedly upregulated during stress. A 62 kDa Hsp (Hsp60) of Histoplasma capsulatum (Hc) is an immunodominant antigen and the major surface ligand to CR3 receptors on macrophages. However little is known about the function of this protein within the fungus. We characterized Hc Hsp60-protein interactions under different temperature to gain insights of its additional functions oncell wall dynamism, heat stress and pathogenesis. We conducted co-immunoprecipitations with antibodies to Hc Hsp60 using cytoplasmic and cell wall extracts. Interacting proteins were identified by shotgun proteomics. For the cell wall, 84 common interactions were identified among the 3 growth conditions, including proteins involved in heat-shock response, sugar and amino acid/protein metabolism and cell signaling. Unique interactions were found at each temperature [30°C (81 proteins), 37°C (14) and 37/40°C (47)]. There were fewer unique interactions in cytoplasm [30°C (6), 37°C (25) and 37/40°C (39)] and four common interactions, including additional Hsps and other known virulence factors. These results show the complexity of Hsp60 function and provide insights into Hc biology, which may lead to new avenues for the management of histoplasmosis