“Gogol or Provincial in the Capital”: from Ukraine to St. Petersburg

The article is devoted to the problem of the uniqueness of creativity of N. V. Gogol and his place in literature. The relevance of the work is determined by pendency of the issue, which literature researchers repeatedly addressed to. The author uses an interdisciplinary approach. The study is based on extensive literary material. Problem of finding the “key” to understanding the creativity and fate of N. V. Gogol rises from the lore point of view. A review of literature on the subject is given. It is shown that in the spiritual and creative fracture of the writer, and hence his growth, a significant role was played by moving from the Ukraine to Petersburg. The author dwells on the universality of Gogol as one of the national corollaries of duality, which became a fruitful imperative of his creativity, the key breakthrough - through Russian language and Russian themes - from the “provincial Dikanka” to the universal horizon. The article proves the idea that Petersburg in contrast with the Little Russia breaks (and forms) Gogol’s optics. It is noted that the writer did not remain in debt: there was “invasion of the Ukrainian wave with Gogol on the crest” in Russian culture (in the words of N. Piksanov). It is shown that the regional approach applicable to the figure of the writer allows to expand the possibilities of literary analysis. Also the question is raised about the relationship of reality and artistic fiction in the works by N. Gogol

Из истории становления терминологии языка саха (на материале словарей изданных в 1920-1980 гг.)

The article has a history of the formation and development of Sakha terminology on the material of dictionaries published from 1920 to 1980. The authors considered the stages: “Formation of the basics of terminological vocabulary” (1920-1940) and “The third industrial (transitional) stage” (1940-1980). The stages of the formation and development of the terminology of the Yakut literary language are described. The analysis of the development of the historical situation of periods is given. Key words: component parts (inventory and interpretation) parameters, modifications, translations and interpretation of the meaning of terms in the form of definitions.Keywords: Yakut language, terminology, dictionary, equivalent, affix, phrases.В статье рассматривается история становления и развития терминологии языка саха на материале словарей изданных с 1920 по 1980 гг. Авторы рассмотрели этапы: «Формирование основ терминологической лексики» (1920-1940 гг.) и «Третий индустриальный (переходный) этап» (1940-1980 гг.). Описываются этапы становления и развития терминологии якутского литературного языка. Дается анализ развития исторической обстановки периодов. Описано качество словников словарей, их композиционные (инвентаризационные и интерпретационные) параметры, модификации приемов перевода и интерпретация значения терминов в виде дефиниций. Ключевые слова: Якутский язык, терминология, словарь, эквивалент, аффикс, словосочетания.

Evaluation of the effect of the furazane fragment on the NH acidity of 4- and 5-picrylaminobenzofurazanes in DMF and DMSO

The products of the reactions of picryl chloride with isomeric aminobenzofurazanes in DMF and DMSO were studied by means of nonaquoeus potentiometric titration. The effect of the position of the furazane fragment in 4- and 5-picrylaminobenzofurazanes on the NH acidity is considered. The electron-acceptor properties of the furazane fragment were evaluated via inclusion of the resulting data into the pK a-σ correlation for 2,4,6-trinitrodiphenylamines. On this basis, conventional Hammett σ constants were calculated for the furazane fragment located in the immediate vicinity of the N-H center and one position distant from it. © 2005 Pleiades Publishing, Inc

The experience of positioning the university at the educational services market

The relevance of the problem under study is based on the necessity to find the most productive method of positioning a higher educational organization at the educational services market within the conditions of transfer from the centrally planned economy to a market economy and changing of the thinking format of the young generation. The purpose of the article is to develop a set of marketing actions for positioning a higher educational organization at the educational services market and its assessment. The lead method of studying this problem is modeling which provides the possibility to consider the problem as a process of results-oriented and well-informed managing of the process of development of a university as an educational system. The article contains a set of marketing actions aimed at positioning the university at the educational services market using the organization’s own internal resources and consisting of the interconnected components, such as motivational-target, content-processing and resultassessing; their specific features are described and the algorithm of implementing this set is developed. The developed and assessed system is intended for promotion of the university as a brand at the educational services market, for strengthening and reinforcing its competitive positions and increasing the university’s attractiveness for future students. The materials of the article can be useful for education specialists oriented towards marketing of educational services, for teachers who deliver a course of lectures in disciplines related to pedagogical marketing or marketing of educational services. © 2016 Dorozhkin et al

High-resolution phase based method for FMCW short range radars

The paper deals with the problem of the using phase-based methods for frequency estimation in the frequency modulated continuum waves (FMCW) short-range radars in the case of high-resolution frequency estimation requirements. The equation is considered for frequency estimation by the least-square method of approximating the phase-to-time relation of the beat signal correlation function in the case of several significant components in the signal (such as valuable and interference signal-like tones). Solution is proposed for the equations by using the parametric (or subspace) decomposition of beat signals (such as eigenvector decomposition, EV). The numerical investigation shows that the bias of the frequency estimations by the proposed solution of the mentioned equation above has statistical properties similar to the method of estimation of signal parameters via rotational invariance techniques (ESPRIT). However, the proposed method does not require the double decompositions, and frequency of each eigenvector can be estimated separately. It is also shown that in the case of the unknown number of signal components the proposed method has the higher statistical properties than for such popular technique as the multiple signal classification method (MUSIC). © 2020 American Institute of Physics Inc.. All rights reserved.The work was supported by Act 211 Government of the Russian Federation, contract N 02.A03.21.0006

Purinergic inhibition of Na+,K+,Cl− cotransport in C11-MDCK cells: Role of stress-activated protein kinases

Previously, we observed that sustained activation of P2Y1 leads to inhibition of Na+,K+,Cl− cotransport (NKCC) in C11 cells resembling intercalated cells from collecting ducts of the Madin-Darby canine kidney. This study examined the role of stress-activated protein kinases (SAPK) in NKCC inhibition triggered by purinergic receptors. Treatment of C11 cells with ATP led to sustained phosphorylation of SAPK such as JNK and p38. Activation of these kinases also occurred in anisomycin-treated cells. Surprisingly, we observed that compounds SP600125 and SB202190, known as potent inhibitors of JNK and p38 in cell-free systems, activated rather than inhibited phosphorylation of the kinases in C11 cells. Importantly, similarly to ATP, all the above-listed activators of JNK and p38 phosphorylation inhibited NKCC. Thus, our results suggest that activation of JNK and/or p38 contributes to NKCC suppression detected in intercalated-like cells from distal tubules after their exposure to P2Y1 agonists

Helios Expression Is a Marker of T Cell Activation and Proliferation

Foxp3+ T-regulatory cells (Tregs) normally serve to attenuate immune responses and are key to maintenance of immune homeostasis. Over the past decade, Treg cells have become a major focus of research for many groups, and various functional subsets have been characterized. Recently, the Ikaros family member, Helios, was reported as a marker to discriminate naturally occurring, thymic-derived Tregs from those peripherally induced from naïve CD4+ T cells. We investigated Helios expression in murine and human T cells under resting or activating conditions, using well-characterized molecules of naïve/effector/memory phenotypes, as well as a set of Treg-associated markers. We found that Helios-negative T cells are enriched for naïve T cell phenotypes and vice versa. Moreover, Helios can be induced during T cell activation and proliferation, but regresses in the same cells under resting conditions. We demonstrated comparable findings using human and murine CD4+Foxp3+ Tregs, as well as in CD4+ and CD8+ T cells. Since Helios expression is associated with T cell activation and cellular division, regardless of the cell subset involved, it does not appear suitable as a marker to distinguish natural and induced Treg cells

Ubiquitous [Na+]i/[K+]i-Sensitive Transcriptome in Mammalian Cells: Evidence for Ca2+i-Independent Excitation-Transcription Coupling

Stimulus-dependent elevation of intracellular Ca2+ ([Ca2+]i) affects the expression of numerous genes – a phenomenon known as excitation-transcription coupling. Recently, we found that increases in [Na+]i trigger c-Fos expression via a novel Ca2+i-independent pathway. In the present study, we identified ubiquitous and tissue-specific [Na+]i/[K+]i-sensitive transcriptomes by comparative analysis of differentially expressed genes in vascular smooth muscle cells from rat aorta (RVSMC), the human adenocarcinoma cell line HeLa, and human umbilical vein endothelial cells (HUVEC). To augment [Na+]i and reduce [K+]i, cells were treated for 3 hrs with the Na+,K+-ATPase inhibitor ouabain or placed for the same time in the K+-free medium. Employing Affymetrix-based technology, we detected changes in expression levels of 684, 737 and 1839 transcripts in HeLa, HUVEC and RVSMC, respectively, that were highly correlated between two treatments (p<0.0001; R2>0.62). Among these Na+i/K+i-sensitive genes, 80 transcripts were common for all three types of cells. To establish if changes in gene expression are dependent on increases in [Ca2+]i, we performed identical experiments in Ca2+-free media supplemented with extracellular and intracellular Ca2+ chelators. Surprisingly, this procedure elevated rather than decreased the number of ubiquitous and cell-type specific Na+i/K+i-sensitive genes. Among the ubiquitous Na+i/K+i-sensitive genes whose expression was regulated independently of the presence of Ca2+ chelators by more than 3-fold, we discovered several transcription factors (Fos, Jun, Hes1, Nfkbia), interleukin-6, protein phosphatase 1 regulatory subunit, dual specificity phosphatase (Dusp8), prostaglandin-endoperoxide synthase 2, cyclin L1, whereas expression of metallopeptidase Adamts1, adrenomedulin, Dups1, Dusp10 and Dusp16 was detected exclusively in Ca2+-depleted cells. Overall, our findings indicate that Ca2+i-independent mechanisms of excitation-transcription coupling are involved in transcriptomic alterations triggered by elevation of the [Na+]i/[K+]i ratio. There results likely have profound implications for normal and pathological regulation of mammalian cells, including sustained excitation of neuronal cells, intensive exercise and ischemia-triggered disorders

Методологічні основи дослідження стану соціоекологоекономічної безпеки

У монографії відображені методологічні основи дослідження стану соціоекологоекономічної безпеки об’єктів мікро-, мезо-, макрорівня. Удосконалено методику оцінки соціальної, економічної, екологічної безпеки та соціоекологоекономічної безпеки об’єктів мікро-, мезо-, макрорівня. Набули подальшого розвитку методологічні підходи до дослідження загального стану та визначення рівня соціоекологоекономічної безпеки населення, підприємства, регіону, держави.Дослідження розраховане на науковців, керівників та фахівців підприємств, установ, організацій, представників державних органів управління, викладачів, студентів, а також кола читачів, які цікавляться проблемами забезпечення соціоекологоекономічної безпеки об’єктів мікро-, мезо-, макрорівня

Murine Pancreatic Adenocarcinoma Reduces Ikaros Expression and Disrupts T Cell Homeostasis

Background Maintenance of T cell immune homeostasis is critical for adequate anti-tumor immunity. The transcription factor Ikaros is essential for lymphocyte development including T cells. Alterations in Ikaros expression occur in blood malignancies in humans and mice. In this study, we investigated the role of Ikaros in regulating T cell immune balance in pancreatic cancer mouse models. Methodology and Principal Findings Using our Panc02 tumor-bearing (TB) mouse model, western blot analysis revealed a reduction in Ikaros proteins while qRT-PCR showed no differences in Ikaros mRNA levels in TB splenocytes compared to control. Treatment of naïve splenocytes with the proteasomal inhibitor, MG132, stabilized Ikaros expression and prevented Ikaros downregulation by Panc02 cells, in vitro. Western blot analyses showed a reduction in protein phosphatase 1 (PP1) and protein kinase CK2 expression in TB splenocytes while CK2 activity was increased. Immunofluorescence microscopy revealed altered punctate staining of Ikaros in TB splenocytes. Flow cytometry revealed a significant decrease in effector CD4+ and CD8+ T cell percentages but increased CD4+CD25+ regulatory T cells in TB splenocytes. Similar alterations in T cell percentages, as well as reduced Ikaros and CK2 but not PP1 expression, were observed in a transgenic, triple mutant (TrM) pancreatic cancer model. Ikaros expression was also reduced in enriched TB CD3+ T cells. MG132 treatment of naïve CD3+ T cells stabilized Ikaros expression in the presence of Panc02 cells. Western blots showed reduced PP1 and CK2 expression in TB CD3+ T cells. Conclusions/Significance The results of this study suggest that the pancreatic tumor microenvironment may cause proteasomal degradation of Ikaros, possibly via dysregulation of PP1 and CK2 expression and activity, respectively. This loss of Ikaros expression may contribute to an imbalance in T cell percentages. Ikaros may potentially be a therapeutic target to restore T cell homeostasis in pancreatic cancer hosts, which may be critical for effective anti-tumor immunity