Field-adapted sampling of whole blood to determine the levels of amodiaquine and its metabolite in children with uncomplicated malaria treated with amodiaquine plus artesunate combination

<p>Abstract</p> <p>Background</p> <p>Artemisinin combination therapy (ACT) has been widely adopted as first-line treatment for uncomplicated falciparum malaria. In Uganda, amodiaquine plus artesunate (AQ+AS), is the alternative first-line regimen to Coartem^®(artemether + lumefantrine) for the treatment of uncomplicated falciparum malaria. Currently, there are few field-adapted analytical techniques for monitoring amodiaquine utilization in patients. This study evaluates the field applicability of a new method to determine amodiaquine and its metabolite concentrations in whole blood dried on filter paper.</p> <p>Methods</p> <p>Twelve patients aged between 1.5 to 8 years with uncomplicated malaria received three standard oral doses of AQ+AS. Filter paper blood samples were collected before drug intake and at six different time points over 28 days period. A new field-adapted sampling procedure and liquid chromatographic method was used for quantitative determination of amodiaquine and its metabolite in whole blood.</p> <p>Results</p> <p>The sampling procedure was successively applied in the field. Amodiaquine could be quantified for at least three days and the metabolite up to 28 days. All parasites in all the 12 patients cleared within the first three days of treatment and no adverse drug effects were observed.</p> <p>Conclusion</p> <p>The methodology is suitable for field studies. The possibility to determine the concentration of the active metabolite of amodiaquine up to 28 days suggested that the method is sensitive enough to monitor amodiaquine utilization in patients. Amodiaquine plus artesunate seems effective for treatment of falciparum malaria.</p

Efeitos do estado de humor sobre os comportamentos alimentares inadequados de atletas de atletismo

O objetivo do presente estudo foi analisar os efeitos do estado de humor sobre os comportamentos alimentares inadequados (CAI) em atletas. Participaram 68 atletas de atletismo, do sexo feminino, com idades entre 12 e 17 anos. Utilizaram-se o Eating Attitudes Test (EAT-26) e a Escala de Humor de Brunel (BRUMS) para avaliar os CAI e o distúrbio total do humor (DTH), respectivamente. Dois modelos de regressão linear não indicaram influência estatisticamente significativa da BRUMS nas subescalas Dieta (p=0,42) e Bulimia e Preocupação com Alimentos (p=0,81) do EAT-26. Ao contrário, a BRUMS (p=0,04) demonstrou impacto significante (7%) sobre os escores da subescala Autocontrole Oral. Concluiu-se que as atletas com DTH elevado podem estar mais susceptíveis à influência ambiental para a ingestão alimentar

Eating attitudes, body esteem, perfectionism and anxiety of judo athletes and nonathletes

International audienceThe aim of the study was to examine the prevalence and relationships between disordered eating, menstrual irregularity, musculoskeletal injuries and psychological characteristics in 24 judo athletes (12 females and 12 males) and 31 controls (14 females and 17 males). All these parameters were assessed by a health/medical, dieting and menstrual history questionnaire, the Eating Attitudes Test (EAT-26), the Multidimensional perfectionism scale, the Rosenberg Self-esteem, the Body esteem scale, and the Profile of Mood States. Body mass index (BMI) was also computed. Twenty-five percent of female athletes would be "at risk" of EDs (EAT-26 > 20) and 0 % in the other sample groups. Bone injuries sustained over the judo athlete career were reported by 25 % of females and 33.3 % of males, while 35.7 % of the female controls reported bone injuries. The total frequency of menstrual dysfunction among judo athletes was 58.3 %, while 7.1 % of female controls reported oligoamenorrhea. Regression analyses showed that BE-Weight Satisfaction and BMI contributed to 54.6 % and 17 % of the variance, respectively, in the prediction of log-transformed Global EAT scores among female judo athletes. These data indicate that while the prevalence of clinical eating disorders is low in judo athletes, many are "at risk" for an eating disorder, which places them at an increased risk for menstrual irregularity and bone injuries. This study also highlights the relevance of body esteem to eating disorder symptoms