Tunable Hydrogel Substrates for Studying Pathogen-Host Cell Interactions

Extracellular matrix elasticity has been shown to affect a variety of cellular properties. However, the effect it has on pathogen-host cell interaction has yet to be thoroughly evaluated. Furthering our understanding of pathogenic infection mechanisms could be beneficial to the development of vaccinations for poorly understood pathogens as well as new therapies to combat a rapidly growing antibiotic-resistant pathogen population. We evaluated two polymer systems in this study: a polyacrylamide hydrogel system that was functionalized for cell culture and a poly(ethylene glycol)-norbornene hydrogel system that utilizes thiol-ene click chemistry for simple and rapid functionalization and tunability. Our pathogen of interest is Brucella melitensis, a bacteria that affects millions of people worldwide, through livestock death, zoonotic infection, and bioterroristic threat. Since Brucella melitensis primarily targets phagocytes, macrophages were utilized for evaluating the success of the hydrogels in hosting tissue culture. It was determined that the poly(ethylene glycol)-norbornene system had the most success in culturing healthy cells and easily tuning mechanical characteristics. Based on these results, future studies in the Alge Lab will involve seeding macrophages onto poly(ethylene glycol)-norbornene gels of varying elastic moduli and infecting them with Brucella to investigate the influence of extracellular matrix elasticity on Brucella infection rate

Boolean subalgebras of orthoalgebras

We develop a direct method to recover an orthoalgebra from its poset of Boolean subalgebras. For this a new notion of direction is introduced. Directions are also used to characterize in purely order-theoretic terms those posets that are isomorphic to the poset of Boolean subalgebras of an orthoalgebra. These posets are characterized by simple conditions defining orthodomains and the additional requirement of having enough directions. Excepting pathologies involving maximal Boolean subalgebras of four elements, it is shown that there is an equivalence between the category of orthoalgebras and the category of orthodomains with enough directions with morphisms suitably defined. Furthermore, we develop a representation of orthodomains with enough directions, and hence of orthoalgebras, as certain hypergraphs. This hypergraph approach extends the technique of Greechie diagrams and resembles projective geometry. Using such hypergraphs, every orthomodular poset can be represented by a set of points and lines where each line contains exactly three points

Kochen-Specker Sets and Generalized Orthoarguesian Equations

Every set (finite or infinite) of quantum vectors (states) satisfies generalized orthoarguesian equations ($n$OA). We consider two 3-dim Kochen-Specker (KS) sets of vectors and show how each of them should be represented by means of a Hasse diagram---a lattice, an algebra of subspaces of a Hilbert space--that contains rays and planes determined by the vectors so as to satisfy $n$OA. That also shows why they cannot be represented by a special kind of Hasse diagram called a Greechie diagram, as has been erroneously done in the literature. One of the KS sets (Peres') is an example of a lattice in which 6OA pass and 7OA fails, and that closes an open question of whether the 7oa class of lattices properly contains the 6oa class. This result is important because it provides additional evidence that our previously given proof of noa =< (n+1)oa can be extended to proper inclusion noa < (n+1)oa and that nOA form an infinite sequence of successively stronger equations.Comment: 16 pages and 5 figure

Cascading Effects of Hunting Disturbance on Northern Bobwhite Behavior, Physiology, and Survival

The northern bobwhite (Colinus virginianus; hereafter, bobwhite) is an important gamebird across the United States and has been in decline for several decades. As a commonly hunted prey species, the bobwhite provides an ideal study species to investigate the use of proactive and reactive antipredator behaviors in response to hunting pressure. We designed an experiment to understand how late-season hunting affects bobwhite demographics using fecal glucocorticoid (fGCM) concentrations, foraging and movement behaviors, survival, and breeding season metrics. Our results show that bobwhite responded to increased interactions with a shotgun through proactive responses. After one encounter with a discharged shotgun, bobwhite began foraging farther from supplemental feed where the risk of encountering a hunting party was the greatest (β = 0.21, 95% Bayesian credible interval [CrI]: 0.06–0.36). Bobwhite responded to increased hunting pressure, particularly late-season hunting pressure, via reactive responses through increased fecal glucocorticoid metabolite (fGCM) concentrations (β = 2.18, 95% CrI: 0.21–4.15), resulting in decreased survivorship in non-harvested individuals (β = -0.42, 95% CrI: -0.77 to -0.07) and decreased fecundity (β = -0.17, 95% CI: -0.31–0.09). These results can help inform hunting season regulations and management decisions aiding in bobwhite recovery

Preimplantation expression of the somatic form of Dnmt1 suggests a role in the inheritance of genomic imprints

BACKGROUND: Identical DNA methylation differences between maternal and paternal alleles in gametes and adults suggest that the inheritance of genomic imprints is strictly due to the embryonic maintenance of DNA methylation. Such maintenance would occur in association with every cycle of DNA replication, including those of preimplantation embryos. RESULTS: The expression of the somatic form of the Dnmt1 cytosine methyltransferase (Dnmt1s) was examined in cleavage-stage preimplantation mouse embryos. Low concentrations of Dnmt1s are found in 1-, 2-, 4-, and 8-cell embryos, as well as in morulae and blastocysts. Dnmt1s is present in the cytoplasm at all stages, and in the nuclei of all stages except the 1-cell, pronuclear-stage embryo. The related oocyte-derived Dnmt1o protein is also present in nuclei of 8-cell embryos, along with embryo-synthesized Dnmt1s. Dnmt1s protein expressed in 1-cell and 2-cell embryos is derived from the oocyte, whereas the embryo synthesizes its own Dnmt1s from the 2-cell stage onward. CONCLUSION: These observations suggest that Dnmt1s provides maintenance methyltransferase activity for the inheritance of methylation imprints in the early mouse embryo. Moreover, the ability of Dnmt1o and Dnmt1s proteins synthesized at the same time to substitute for one another's maintenance function, but the lack of functional interchange between oocyte- and embryo-synthesized Dnmt1 proteins, suggests that the developmental source is the critical determinant of Dnmt1 function during preimplantation development

Livestock abundance predicts vampire bat demography, immune profiles, and bacterial infection risk

Human activities create novel food resources that can alter wildlife–pathogen interactions. If resources amplify or dampen, pathogen transmission probably depends on both host ecology and pathogen biology, but studies that measure responses to provisioning across both scales are rare. We tested these relationships with a 4-year study of 369 common vampire bats across 10 sites in Peru and Belize that differ in the abundance of livestock, an important anthropogenic food source. We quantified innate and adaptive immunity from bats and assessed infection with two common bacteria. We predicted that abundant livestock could reduce starvation and foraging effort, allowing for greater investments in immunity. Bats from high-livestock sites had higher microbicidal activity and proportions of neutrophils but lower immunoglobulin G and proportions of lymphocytes, suggesting more investment in innate relative to adaptive immunity and either greater chronic stress or pathogen exposure. This relationship was most pronounced in reproductive bats, which were also more common in high-livestock sites, suggesting feedbacks between demographic correlates of provisioning and immunity. Infection with both Bartonella and haemoplasmas were correlated with similar immune profiles, and both pathogens tended to be less prevalent in high-livestock sites, although effects were weaker for haemoplasmas. These differing responses to provisioning might therefore reflect distinct transmission processes. Predicting how provisioning alters host–pathogen interactions requires considering how both within-host processes and transmission modes respond to resource shifts

Hilbert Lattice Equations

There are five known classes of lattice equations that hold in every infinite dimensional Hilbert space underlying quantum systems: generalised orthoarguesian, Mayet's E_A, Godowski, Mayet-Godowski, and Mayet's E equations. We obtain a result which opens a possibility that the first two classes coincide. We devise new algorithms to generate Mayet-Godowski equations that allow us to prove that the fourth class properly includes the third. An open problem related to the last class is answered. Finally, we show some new results on the Godowski lattices characterising the third class of equations.Comment: 24 pages, 3 figure

Influence of permanent night work on the circadian rhythm of blood pressure

Abstract. Night workers exercise their labours activities and rest in contrary schedules to the chronobiological standards. This inversion leads the body to several adaptations, including changes in the circadian rhythm of blood pressure (BP). Objectives: To evaluate the BP in individuals who perform work at night, in order to objectively detail the BP circadian rhythm adaptations infixed night workers. Methods: A cross-sectional study enrolling 23 fixed night workers, both genders, was performed, with 24h BP measured with ambulatory blood pressure monitoring (ABPM) during a normal working day. Risk factors, anthropometric and lifestyle information were collected using a standard questionnaire. Results: Ambulatory BP demonstrated a pattern of adaptation to the sleep/activity cycle in all participants. BP dropped during the sleeping period (mean drop: -11.35±6.85) and was higher during the awakening period, reaching the highest results and greater BP variability during the working period. The chronobiological adaptation of the 24h BP was not dependent on sociodemographic or clinical characteristics. In addition, age, male gender, obesity, and those working less time were associated with higher BP mean values. Conclu-sions: The circadian rhythm of BP follows the working circadian profile of the individual.info:eu-repo/semantics/publishedVersio