Dynamic Light Scattering from Semidilute Actin Solutions: A Study of Hydrodynamic Screening, Filament Bending Stiffness and the Effect of Tropomyosin/Troponin-Binding

Quasi-elastic light scattering (QELS) is applied to investigate the effect of the tropomyosin/troponin complex (Tm/Tn) on the stiffness of actin filaments. The importance of hydrodynamic screening in semidilute solutions is demonstrated. A new concentration dependent expression for the dynamic structure factor $g(\bm k,t)$ of semiflexible polymers in semidilute solutions is used to analyze the experimental QELS data. A concentration independent value for the bending modulus $\kappa$ is thus obtained. It increases by 50\% as a consequence of Tm/Tn binding in a 7:1:1 molar ratio of actin/Tm/Tn. In addition a new expression for the initial slope of the dynamic structure factor of a semiflexible polymer is used to determine the effective hydrodynamic diameter of the actin filament. Our results confirm the general relevance of the concept of (intrinsic) semiflexibility to polymer dynamics.Comment: 9 pages, RevTeX, 9 figures, all uuencoded gzipe

Longitudinal Response of Confined Semiflexible Polymers

The longitudinal response of single semiflexible polymers to sudden changes in externally applied forces is known to be controlled by the propagation and relaxation of backbone tension. Under many experimental circumstances, realized, e.g., in nano-fluidic devices or in polymeric networks or solutions, these polymers are effectively confined in a channel- or tube-like geometry. By means of heuristic scaling laws and rigorous analytical theory, we analyze the tension dynamics of confined semiflexible polymers for various generic experimental setups. It turns out that in contrast to the well-known linear response, the influence of confinement on the non-linear dynamics can largely be described as that of an effective prestress. We also study the free relaxation of an initially confined chain, finding a surprising superlinear t^(9/8) growth law for the change in end-to-end distance at short times.Comment: 18 pages, 1 figur

The spillover effects of monitoring:A field experiment

Published Online: March 13, 2015We provide field experimental evidence of the effects of monitoring in a context where productivity is multidimensional and only one dimension is monitored and incentivized. We hire students to do a job for us. The job consists of identifying euro coins. We study the direct effects of monitoring and penalizing mistakes on work quality and evaluate spillovers on unmonitored dimensions of productivity (punctuality and theft). We find that monitoring improves work quality only if incentives are harsh, but substantially reduces punctuality irrespectively of the associated incentives. Monitoring does not affect theft, with 10% of participants stealing overall. Our findings are supportive of a reciprocity mechanism, whereby workers retaliate for being distrusted

Characterization of a microwave frequency resonator via a nearby quantum dot

We present measurements of a hybrid system consisting of a microwave transmission-line resonator and a lateral quantum dot defined on a GaAs heterostructure. The two subsystems are separately characterized and their interaction is studied by monitoring the electrical conductance through the quantum dot. The presence of a strong microwave field in the resonator is found to reduce the resonant conductance through the quantum dot, and is attributed to electron heating and modulation of the dot potential. We use this interaction to demonstrate a measurement of the resonator transmission spectrum using the quantum dot.Comment: 3 pages, 3 figure

Atmospheric nitrogen oxides (NO and NO2) at Dome C, East Antarctica, during the OPALE campaign

Mixing ratios of the atmospheric nitrogen oxides NO and NO2 were measured as part of the OPALE (Oxidant Production in Antarctic Lands & Export) campaign at Dome C, East Antarctica (75.1 degrees S, 123.3 degrees E, 3233 m), during December 2011 to January 2012. Profiles of NOx mixing ratios of the lower 100m of the atmosphere confirm that, in contrast to the South Pole, air chemistry at Dome C is strongly influenced by large diurnal cycles in solar irradiance and a sudden collapse of the atmospheric boundary layer in the early evening. Depth profiles of mixing ratios in firn air suggest that the upper snowpack at Dome C holds a significant reservoir of photolytically produced NO2 and is a sink of gas-phase ozone (O-3). First-time observations of bromine oxide (BrO) at Dome C show that mixing ratios of BrO near the ground are low, certainly less than 5 pptv, with higher levels in the free troposphere. Assuming steady state, observed mixing ratios of BrO and RO2 radicals are too low to explain the large NO2 : NO ratios found in ambient air, possibly indicating the existence of an unknown process contributing to the atmospheric chemistry of reactive nitrogen above the Antarctic Plateau. During 2011-2012, NOx mixing ratios and flux were larger than in 2009-2010, consistent with also larger surface O-3 mixing ratios resulting from increased net O-3 production. Large NOx mixing ratios at Dome C arise from a combination of continuous sunlight, shallow mixing height and significant NOx emissions by surface snow (F-NOx). During 23 December 2011-12 January 2012, median F-NOx was twice that during the same period in 20092010 due to significantly larger atmospheric turbulence and a slightly stronger snowpack source. A tripling of F-NOx in December 2011 was largely due to changes in snowpack source strength caused primarily by changes in NO3- concentrations in the snow skin layer, and only to a secondary order by decrease of total column O-3 and associated increase in NO3- photolysis rates. A source of uncertainty in model estimates of F-NOx is the quantum yield of NO3- photolysis in natural snow, which may change over time as the snow ages

Relationship between Groat-Oil Content and Grain Yield of Oats (Avena sativa L.)

Raising the energy content of oat (Avena sativa L.) groats by increasing their oil percentage could improve the economic value of oats as a feed grain and as a source of culinary oil. Relative importance of genotype x environment interaction for groat-oil content and grain yield and the correlations of groat-oil content with yield and maturity were evaluated for ten cultivars grown at three Iowa locations for two years. Cultivar x location mean squares were significant for both traits, but they were much smaller, relative to cultivar mean squares, for groat-oil content than for grain yield. Ranking of oat cultivars for groat-oil content was similar in all environments. Groat-oil content was positively correlated with grain yield in both years (r = 0.62* and 0.63*) and had a negative but nonsignificant correlation coefficient with maturity (r = - 0.28 and - 0.48). These relationships are favorable for the development of high-yielding, high groat-oil content cultivars

Scaling regimes and critical dimensions in the Kardar-Parisi-Zhang problem

We study the scaling regimes for the Kardar-Parisi-Zhang equation with noise correlator R(q) ~ (1 + w q^{-2 \rho}) in Fourier space, as a function of \rho and the spatial dimension d. By means of a stochastic Cole-Hopf transformation, the critical and correction-to-scaling exponents at the roughening transition are determined to all orders in a (d - d_c) expansion. We also argue that there is a intriguing possibility that the rough phases above and below the lower critical dimension d_c = 2 (1 + \rho) are genuinely different which could lead to a re-interpretation of results in the literature.Comment: Latex, 7 pages, eps files for two figures as well as Europhys. Lett. style files included; slightly expanded reincarnatio

Scaling and universality in coupled driven diffusive models

Inspired by the physics of magnetohydrodynamics (MHD) a simplified coupled Burgers-like model in one dimension (1d), a generalization of the Burgers model to coupled degrees of freedom, is proposed to describe 1dMHD. In addition to MHD, this model serves as a 1d reduced model for driven binary fluid mixtures. Here we have performed a comprehensive study of the universal properties of the generalized d-dimensional version of the reduced model. We employ both analytical and numerical approaches. In particular, we determine the scaling exponents and the amplitude-ratios of the relevant two-point time-dependent correlation functions in the model. We demonstrate that these quantities vary continuously with the amplitude of the noise cross-correlation. Further our numerical studies corroborate the continuous dependence of long wavelength and long time-scale physics of the model on the amplitude of the noise cross-correlations, as found in our analytical studies. We construct and simulate lattice-gas models of coupled degrees of freedom in 1d, belonging to the universality class of our coupled Burgers-like model, which display similar behavior. We use a variety of numerical (Monte-Carlo and Pseudospectral methods) and analytical (Dynamic Renormalization Group, Self-Consistent Mode-Coupling Theory and Functional Renormalization Group) approaches for our work. The results from our different approaches complement one another. Possible realizations of our results in various nonequilibrium models are discussed.Comment: To appear in JSTAT (2009); 52 pages in JSTAT format. Some figure files have been replace

Canonical phase space approach to the noisy Burgers equation

Presenting a general phase approach to stochastic processes we analyze in particular the Fokker-Planck equation for the noisy Burgers equation and discuss the time dependent and stationary probability distributions. In one dimension we derive the long-time skew distribution approaching the symmetric stationary Gaussian distribution. In the short time regime we discuss heuristically the nonlinear soliton contributions and derive an expression for the distribution in accordance with the directed polymer-replica model and asymmetric exclusion model results.Comment: 4 pages, Revtex file, submitted to Phys. Rev. Lett. a reference has been added and a few typos correcte

VLBI imaging of extremely high redshift quasars at 5 GHz

We present very long baseline interferometry (VLBI) images of ten very high redshift (z>3) quasars at 5 GHz. The sources 0004+139, 0830+101, 0906+041, 0938+119 and 1500+045 were observed in September 1992 using a global VLBI array, while 0046+063, 0243+181, 1338+381, 1428+423 and 1557+032 were observed in October 1996 with the European VLBI Network and Hartebeesthoek, South Africa. Most of the sources are resolved and show asymmetric structure. The sample includes 1428+423, the most distant radio loud quasar known to date (z=4.72). It is barely resolved with an angular resolution of about 2.0*1.4 mas.Comment: Astronomy and Astrophysics, in press, Latex2e, 10 pages, 3 figures (and lots of sub-figures