Deep Proteomics of Mouse Skeletal Muscle Enables Quantitation of Protein Isoforms, Metabolic Pathways, and Transcription Factors

Skeletal muscle constitutes 40% of individual body mass and plays vital roles in locomotion and whole-body metabolism. Proteomics of skeletal muscle is challenging because of highly abundant contractile proteins that interfere with detection of regulatory proteins. Using a state-of-the art MS workflow and a strategy to map identifications from the C2C12 cell line model to tissues, we identified a total of 10,218 proteins, including skeletal muscle specific transcription factors like myod1 and myogenin and circadian clock proteins. We obtain absolute abundances for proteins expressed in a muscle cell line and skeletal muscle, which should serve as a valuable resource. Quantitation of protein isoforms of glucose uptake signaling pathways and in glucose and lipid metabolic pathways provides a detailed metabolic map of the cell line compared with tissue. This revealed unexpectedly complex regulation of AMP-activated protein kinase and insulin signaling in muscle tissue at the level of enzyme isoforms

Magnetic Switching of Phase-Slip Dissipation in NbSe2 Nanobelts

The stability of the superconducting dissipationless and resistive states in single-crystalline NbSe2 nanobelts is characterized by transport measurements in an external magnetic field (H). Current-driven electrical measurements show voltage steps, indicating the nucleation of phase-slip structures. Well below the critical temperature, the position of the voltage steps exhibits a sharp, periodic dependence as a function of H. This phenomenon is discussed in the context of two possible mechanisms: the interference of the order parameter and the periodic rearrangement of the vortex lattice within the nanobelt.Comment: 4 figure

Bumper catch of silver pomfret Pampus argenteus at Satpati, Bombay

The article provies details about heavy catches of silver pomfret Pampus argenteus within a span of four days at Satpati, a fishing village in Thane District in Maharashtra during 1987

Solving rate equations for electron tunneling via discrete quantum states

We consider the form of the current-voltage curves generated when tunneling spectroscopy is used to measure the energies of individual electronic energy levels in nanometer-scale systems. We point out that the voltage positions of the tunneling resonances can undergo temperature-dependent shifts, leading to errors in spectroscopic measurements that are proportional to temperature. We do this by solving the set of rate equations that can be used to describe electron tunneling via discrete quantum states, for a number of cases important for comparison to experiments, including (1) when just one spin-degenerate level is accessible for transport, (2) when 2 spin-degenerate levels are accessible, with no variation in electron-electron interactions between eigenstates, and (3) when 2 spin-degenerate levels are accessible, but with variations in electron-electron interactions. We also comment on the general case with an arbitrary number of accessible levels. In each case we analyze the voltage-positions, amplitudes, and widths of the current steps due to the quantum states.Comment: REVTeX 4, 10 pages, 12 figures, submitted to Phys. Rev. B. Associated programs available at http://www.ccmr.cornell.edu/~ralph

Imaging features of rare mesenychmal liver tumours: beyond haemangiomas.

Tumours arising from mesenchymal tissue components such as vascular, fibrous and adipose tissue can manifest in the liver. Although histopathology is often necessary for definitive diagnosis, many of these lesions exhibit characteristic imaging features. The radiologist plays an important role in suggesting the diagnosis, which can direct appropriate immunohistochemical staining at histology. The aim of this review is to present clinical and imaging findings of a spectrum of mesenchymal liver tumours such as haemangioma, epithelioid haemangioendothelioma, lipoma, PEComa, angiosarcoma, inflammatory myofibroblastic tumour, solitary fibrous tumour, leiomyoma, leiomyosarcoma, Kaposi sarcoma, mesenchymal hamartoma, undifferentiated embryonal sarcoma, rhabdomyosarcoma and hepatic metastases. Knowledge of the characteristic features of these tumours will aid in guiding the radiologic diagnosis and appropriate patient management

Magnetotransport properties of individual InAs nanowires

We probe the magnetotransport properties of individual InAs nanowires in a field effect transistor geometry. In the low magnetic field regime we observe magnetoresistance that is well described by the weak localization (WL) description in diffusive conductors. The weak localization correction is modified to weak anti-localization (WAL) as the gate voltage is increased. We show that the gate voltage can be used to tune the phase coherence length ($l_\phi$) and spin-orbit length ($l_{so}$) by a factor of $\sim$ 2. In the high field and low temperature regime we observe the mobility of devices can be modified significantly as a function of magnetic field. We argue that the role of skipping orbits and the nature of surface scattering is essential in understanding high field magnetotransport in nanowires

Star formation in galaxies at z~4-5 from the SMUVS survey: a clear starburst/main-sequence bimodality for Halpha emitters on the SFR-M* plane

We study a large galaxy sample from the Spitzer Matching Survey of the UltraVISTA ultra-deep Stripes (SMUVS) to search for sources with enhanced 3.6 micron fluxes indicative of strong Halpha emission at z=3.9-4.9. We find that the percentage of "Halpha excess" sources reaches 37-40% for galaxies with stellar masses log10(M*/Msun) ~ 9-10, and decreases to <20% at log10(M*/Msun) ~ 10.7. At higher stellar masses, however, the trend reverses, although this is likely due to AGN contamination. We derive star formation rates (SFR) and specific SFR (sSFR) from the inferred Halpha equivalent widths (EW) of our "Halpha excess" galaxies. We show, for the first time, that the "Halpha excess" galaxies clearly have a bimodal distribution on the SFR-M* plane: they lie on the main sequence of star formation (with log10(sSFR/yr^{-1})<-8.05) or in a starburst cloud (with log10(sSFR/yr^{-1}) >-7.60). The latter contains ~15% of all the objects in our sample and accounts for >50% of the cosmic SFR density at z=3.9-4.9, for which we derive a robust lower limit of 0.066 Msun yr^{-1} Mpc^{-3}. Finally, we identify an unusual >50sigma overdensity of z=3.9-4.9 galaxies within a 0.20 x 0.20 sq. arcmin region. We conclude that the SMUVS unique combination of area and depth at mid-IR wavelengths provides an unprecedented level of statistics and dynamic range which are fundamental to reveal new aspects of galaxy evolution in the young Universe.Comment: 18 pages, 11 figures, 1 table. Re-submitted to the ApJ, after addressing referee report. Main changes with respect to v1: a new section and a new appendix have been added to investigate further the origin and robustness of the sSFR bimodality. No conclusion change

Periosteal Osteosarcoma: A Review of 17 Cases with Mean Follow-up of 52 Months

Purpose: Periosteal osteosarcomas are rare cartilage-rich bone tumours characterized by a juxtacortical eccentric position and are normally regarded oncologically as of intermediate to high grade.Their low incidence is mirrored by a small number of reported cases in the world literature. While there is general agreement that wide surgical excision is required, there is a paucity of evidence regarding adjuvant therapy. Previous reports have not indicated any consistent approach to this to allow appraisal

Intense blue-emitting Ca5Al8O14 : Eu phosphor for mercury free lamp

The calcium aluminates doped with Eu ions, Ca5Al8O14 : Eu, phosphors are prepared by the combustion method. The formation of crystalline aluminates was confirmed by X-ray diffraction pattern. The prepared phosphors were characterized by SEM, TGA, DTA, particle size analyzer and Photoluminescence (PL) techniques. From the UV-excited luminescence spectra it was found that the Eu ions acts as a luminescent centre with luminescence at the blue (λmax = 470 nm) region due to 4f 65d1 → 4f 7 transition. The excitation spectra show the broad band at 355 nm wavelength (λem = 470 nm). The excitation 355 nm is a mercury free excitation and therefore Ca5Al8O14 : Eu may be useful for the solid state lighting phosphor in lamp industry.Intense blue-emitting Ca5Al8O14 : Eu phosphor for mercury free lamp Abhay D Deshmukh1*, S J Dhoble1, S V Godbole2, M K Bhide2 and D R Peshwe3 1Kamla Nehru College, Sakkardara Square, Nagpur-440 009, Maharashtra, India 2Spectroscopy Section, Radiochemistry Division, Bhabha Atomic Research Centre, Mumbai-400 085, India 3Department of Metallurgical and Materials Engineering, VNIT, Nagpur-440 011, Maharashtra, India E-mail : [email protected] Nehru College, Sakkardara Square, Nagpur-440 009, Maharashtra, India 2Spectroscopy Section, Radiochemistry Division, Bhabha Atomic Research Centre, Mumbai-400 085, India 3Department of Metallurgical and Materials Engineering, VNIT, Nagpur-440 011, Maharashtra, Indi

Magnetic Anisotropy Variations and Non-Equilibrium Tunneling in a Cobalt Nanoparticle

We present detailed measurements of the discrete electron-tunneling level spectrum within nanometer-scale cobalt particles as a function of magnetic field and gate voltage, in this way probing individual quantum many-body eigenstates inside ferromagnetic samples. Variations among the observed levels indicate that different quantum states within one particle are subject to different magnetic anisotropy energies. Gate-voltage studies demonstrate that the low-energy tunneling spectrum is affected dramatically by the presence of non-equilibrium spin excitations