A Porosity Method to Describe Complex 3D-Structures Theory and Application to an Explosion

A theoretical method was developed to be able to describe the influence of structures of complex shape on a transient fluid flow without meshing the structures. Structures are considered as solid pores inside the fluid and act as an obstacle for the flow. The method was specifically adapted to fast transient cases.The porosity method was applied to the simulation of a Hypothetical Core Disruptive Accident in a small-scale replica of a Liquid Metal Fast Breeder Reactor. A 2D-axisymmetrical simulation of the MARS test was performed with the EUROPLEXUS code. Whereas the central internal structures of the mock-up could be described with a classical shell model, the influence of the 3D peripheral structures was taken into account with the porosity method.

Cryotolerance of equine spermatozoa correlates with specific fatty acid pattern: A pilot study

Sperm cryopreservation represents a powerful tool for horse breeding. To improve the efficiency of artificial insemination in the horse using cryopreserved spermatozoa, an adequate understanding of the underlying biophysical properties that affect sperm cryosurvival needs to be reached yet. In this pilot study, we described isolation and analysis of the main fatty acids from sperms of stallions classified as good and poor freezers (7 GF and 5 PF, according to sperm motility and viability, before and after cryopreservation). Fatty acid profiles were only assessed in pre-thaw sperms. Eight main fatty acids were identified, using gas chromatography, and their contents were expressed as percentage of the total lipid content. We found that lauric, myristic and oleic acid (C12:0, C14:0 and C18:1n9c) turned out to be about 2-fold more abundant in the sperm cells of the GFs compared with PFs. Moreover, we described for the first time the presence of a very high amount of a trans geometrical isomer of linoleic acid, linolelaidic acid (C18:2n6t), in pre-thaw PF spermatozoa. Notably, we found in fresh sperms of PF stallions a ratio of unsaturated fatty acids to saturated fatty acids which was twice that of those of GF group, suggesting a positive effect of a high saturated-to-unsaturated fatty acid ratio for the “freezability” of equine spermatozoa. Finally, principal component analysis (PCA) confirmed the relationships between specific fatty acids and cryotolerance of equine spermatozoa, also providing a graphical classification and additional information about the dominant variables governing the classification process

"Outside, it is snowing": Experience and finitude in the nonrepresentational landscapes of Alain Robbe-Grillet

Copyright © 2008 PionRomanillos J L, 2008. The definitive, peer-reviewed and edited version of this article is published in Environment and Planning D: Society and Space 26(5) 795 – 822 DOI: 10.1068/d6207This paper presents and explicates the anonymous and impersonal spatialities tentatively mapped in the novels of Alain Robbe-Grillet. Emerging from the kinds of landscapes and visualities articulated, these spatialities are at odds with the kind of anthropocentrism characteristic of phenomenological narratives of spatial experience that would start from an apparently stable human-subject position. It is argued that his body of literature dismantles the anthropocentric narratives and biographies that would produce in both the space of the world and the ‘phenomenological subject’ an unwarranted depth and naturalism. Importantly, and reflecting the theoretical turn towards the being of language, Robbe-Grillet questions the legitimacy of linguistic subjects to capture the spaces of the visible. As such, it is argued that his literature reflects an experience of the critiques of phenomenology. Importantly, this ‘critique’ goes hand in hand with the kinds of spatialities and landscapes that are rendered in the novels—the indefinite perspectives they open up, the paradoxical visualities they sustain or deny, and the disorientation they inject into the heart of spatial experience. These literary effects produce a nonanthropocentric and nonpersonal spatiality which, although contributing to an erasure of the ‘subject’, at the same time expose and open up a sociospatiality based on singularities, intensities, and finitude

Natural Occurrence of Ochratoxin A in Blood and Milk Samples from Jennies and Their Foals after Delivery

An assessment of the natural ochratoxin A (OTA) exposure of seven Martina Franca jennies was carried out by analyzing blood and milk samples collected close to and after delivery. A total of 41 and 34 blood samples were collected from jennies and foals, respectively, and analyzed by ELISA. A total of 33 milk samples were collected from jennies and analyzed by the HPLC/FLD method based on IAC clean-up. Furthermore, 53 feed samples were collected from January to September and analyzed by a reference method (AOAC Official Method No. 2000.03) for OTA content. Feed samples showed OTA levels up to 2.7 ng/g with an incidence of 32%, while the OTA incidence rate in jennies' blood samples was 73%, with a median value of 97 ng/L and concentrations ranging fro

Further Evidence for Chemical Fractionation from Ultraviolet Observations of Carbon Monoxide

Ultraviolet absorption from interstellar 12CO and 13CO was detected toward rho Oph A and chi Oph. The measurements were obtained at medium resolution with the Goddard High Resolution Spectrograph on the Hubble Space Telescope. Column density ratios, N(12CO)/N(13CO), of 125 \pm 23 and 117 \pm 35 were derived for the sight lines toward rho Oph A and chi Oph, respectively. A value of 1100 \pm 600 for the ratio N(12C16O)/N(12C18O) toward rho Oph A was also obtained. Absorption from vibrationally excited H_2 (v" = 3) was clearly seen toward this star as well. The ratios are larger than the isotopic ratios for carbon and oxygen appropriate for ambient interstellar material. Since for both carbon and oxygen the more abundant isotopomer is enhanced, selective isotopic photodissociation plays the key role in the fractionation process for these directions. The enhancement arises because the more abundant isotopomer has lines that are more optically thick, resulting in more self shielding from dissociating radiation. A simple argument involving the amount of self shielding [from N(12CO)] and the strength of the ultraviolet radiation field premeating the gas (from the amount of vibrationally excited H_2) shows that selective isotopic photodissociation controls the fractionation seen in these two sight lines, as well as the sight line to zeta Oph.Comment: 40 pages, 8 figures, to appear in 10 July 2003 issue of Ap

Ochratoxin A affects oocyte maturation and subsequent embryo developmental dynamics in the juvenile sheep model

The genotoxic and nephrotoxic mycotoxin Ochratoxin A (OTA) has also been reported to have adverse effects on oocyte maturation and embryo development. Previous studies on the effects of OTA on female fertility have used micromolar concentrations, but no information is available to date on effects in a more relevant nanomolar range. This study used a juvenile sheep model to evaluate the effects of oocyte exposure to low levels of OTA on maturation, fertilization, and embryo development. Further, it was investigated whether different mechanisms of action of OTA could be responsible for varying toxic effects at different levels of exposure. Cumulus-oocyte-complexes (COCs) were exposed to 10 μmol/L–0.1 nmol/L OTA during in vitro maturation and evaluated for cumulus viability, oocyte maturation, and bioenergetic/oxidative status. COCs were subjected to in vitro fertilization, embryo culture, and embryo quality assessment via morphology, viability, bioenergetic/oxidative status, and time-lapse monitoring. At micromolar concentrations, OTA induced cytotoxic effects, by reducing cumulus expansion and oocyte maturation. OTA altered temporospatial dynamics of zygote pronuclear formation and embryo morphokinetics. Blastocysts, even morphologically normal, were found to undergo collapse events, which were probably related to boosted blastocyst mitochondrial activity. At nanomolar concentrations, OTA did not affect COC morpho-functional parameters, but impaired oocyte ability to prevent polyspermy and increased blastocyst apoptosis. In conclusion, in the female germ cell, cytotoxic nonspecific effects characterize OTA-induced toxicity at high exposure levels, whereas fine tuning-mode effects, not associated with altered cell viability and integrity, characterize OTA toxic action at low levels

Residual Stress Determination of Cast Aluminium Benchmark Components Using Strain Relief Techniques

Background: Residual stress development in precipitation strengthened aluminium foundry alloys has seen little attention, despite the prevalence of their use over a wide array of applications. Objective: This study aims at the evaluation of the residual stress in a cast aluminium benchmark that develops during precipitation heat treatment and determines the preferable stress relaxing techniques for such applications.Methods: The stress states in the as-cast, T4 and T6 tempers of the same AlSi7Cu0.5Mg (A356 with 0.5 wt% Cu) sample were determined through a novel application of the contour method, standard hole drilling, deep hole drilling and incremental deep hole drilling. Results: The results of all measurement techniques lie within approximately 40 MPa for all regions available for comparison, with the greatest differences occurring between the contour method and deep hole drilling for the T6 component. It is shown that the peak tensile residual stresses are almost identical between the heat-treated components (75 MPa), but the distribution and magnitude of compressive residual stress are found to be significantly different. Conclusions: Among the measurement techniques evaluated, the contour method and incremental hole drilling are found to be more suitable for T6 temper, while all techniques perform equally well for T4 temper due to its relatively low strength. It is hypothesised that the difference between the as-cast and heat-treated samples is due to solution heat treatment and quenching, while the difference in T4 and T6 tempers is attributed to the response to ageing

Strong near-infrared emission in the sub-AU disk of the Herbig Ae star HD 163296: evidence of refractory dust?

This is the author accepted manuscript. The final version is available from EDP Sciences via the DOI in this record.We present new long-baseline spectro-interferometric observations of the Herbig Ae star HD 163296 (MWC 275) obtained in the H and K bands with the AMBER instrument at the VLTI. The observations cover a range of spatial resolutions between ∼3 and ∼12 milliarcseconds, with a spectral resolution of ∼30. With a total of 1481 visibilities and 432 closure phases, they represent the most comprehensive (u, v) coverage achieved so far for a young star. The circumstellar material is resolved at the sub-AU spatial scale and closure phase measurements indicate a small but significant deviation from point-symmetry. We discuss the results assuming that the near-infrared excess in HD 163296 is dominated by the emission of a circumstellar disk. A successful fit to the spectral energy distribution, near-infrared visibilities and closure phases is found with a model in which a dominant contribution to the H and K band emission originates in an optically thin, smooth and point-symmetric region extending from about 0.1 to 0.45 AU. At a distance of 0.45 AU from the star, silicates condense, the disk becomes optically thick and develops a puffed-up rim, whose skewed emission can account for the non-zero closure phases. We discuss the source of the inner disk emission and tentatively exclude dense molecular gas as well as optically thin atomic or ionized gas as its possible origin. We propose instead that the smooth inner emission is produced by very refractory grains in a partially cleared region, extending to at least ∼0.5 AU. If so, we may be observing the disk of HD 163296 just before it reaches the transition disk phase. However, we note that the nature of the refractory grains or, in fact, even the possibility of any grain surviving at the very high temperatures we require (∼2100−2300 K at 0.1 AU from the star) is unclear and should be investigated further.We acknowledge fundings from CNRS and INAF (grant ASI-INAF I/016/07/0). This work was in part performed under contract with the Jet Propulsion Laboratory (JPL) funded by NASA through the Michelson Fellowship Progra

Strong near-infrared emission in the sub-AU disk of the Herbig Ae star HD163296: evidence for refractory dust?

We present new long-baseline spectro-interferometric observations of the HerbigAe star HD163296 obtained in the H and K bands with the AMBER instrument at VLTI. The observations cover a range of spatial resolutions between 3 and 12 milli-arcseconds, with a spectral resolution of ~30. With a total of 1481 visibilities and 432 closure phases, they result in the best (u,v) coverage achieved on a young star so far. The circumstellar material is resolved at the sub-AU spatial scale and closure phase measurements indicate a small but significant deviation from point-symmetry. We discuss the results assuming that the near-infrared excess in HD163296 is dominated by the emission of a circumstellar disk. A successful fit to the spectral energy distribution, near-infrared visibilities and closure phases is found with a model where a dominant contribution to the H and K band emissions arises from an optically thin, smooth and point-symmetric region extending from about 0.1 to 0.45 AU. At the latter distance from the star, silicates condense, the disk becomes optically thick and develops a puffed-up rim, whose skewed emission can account for the non-zero closure phases. We discuss the nature of the inner disk emission and tentatively rule out dense molecular gas as well as optically thin atomic or ionized gas as its possible origin. We propose instead that the inner emission traces the presence of very refractory grains in a partially cleared region, extending at least to 0.5 AU. If so, we may be observing the disk of HD163296 just before it reaches the transition disk phase. However, we note that the nature of the refractory grains or even the possibility for any grain to survive at the very high temperatures we require (~2100-2300 K at 0.1 AU from the star) is unclear and should be investigated further.Comment: 14 pages; 12 figures; accepted by A&

First AMBER/VLTI observations of hot massive stars

AMBER is the first near infrared focal instrument of the VLTI. It combines three telescopes and produces spectrally resolved interferometric measures. This paper discusses some preliminary results of the first scientific observations of AMBER with three Unit Telescopes at medium (1500) and high (12000) spectral resolution. We derive a first set of constraints on the structure of the circumstellar material around the Wolf Rayet Gamma2 Velorum and the LBV Eta Carinae