ApoE-deficient mice and fenritinide: a structural study of the skin

Fenretinide, a synthetic retinoid derivative first investigated for cancer prevention and treatment, has been shown to ameliorate glucose tolerance and the plasma lipid profile, and to reduce body fat mass. Since these effects, together with its ability to inhibit ceramide synthesis, have suggested that fenretinide may display anti-atherosclerotic effects, the purpose of our work was to evaluate the effect of fenretinide on accumulation of lipids on the skin of a dyslipidemic mouse model that spontaneously develops atherosclerosis. To this aim, 9-weeks-old apoE-knockout (EKO) female mice were fed for 12 weeks with a Western diet, without (control) or with (0.1% w/w) fenretinide. At sacrifice, skin biopsies were excised from the thoracic region, dissected in smaller fragments and processed for structural morphology analysis on both paraffin and semithin sections. As a reference, wild-type (WT) mice were likewise treated. Morphological analysis did not show any significant difference between the skin of treated and untreated WT mice. In both the experimental groups, indeed, the epidermis appeared build-up of ordinated overlapped layers of cells and in the dermis there were no signs of alteration. The presence of foam cells was detected only in EKO mice treated and untreated. Other morphological alterations were also visible, although shared almost equally in EKO-Ctrl and EKO-Fen animals. Our data suggest that fenretinide slightly interferes with lipid accumulation in the skin of EKO mice

Risk factors for monozygotic twinning after in vitro fertilization : a systematic review and meta-analysis

Objectives: To establish the risk factors for monozygotic twin (MZT) and monochorionic twin (MCT) pregnancies after in vitro fertilization (IVF). Design: Systematic review and meta-analysis. Setting: Not applicable. Patient(s): Women who achieved MZT and non-MZT pregnancies through IVF. Intervention(s): Systematic search of Medline from January 1995 to October 2018 with cross-checking of references from relevant articles in English. Main Outcome Measure(s): Possible risk factors for MZT or MCT pregnancies after IVF, comprising extended embryo culture, insemination method (conventional IVF and intracytoplasmic sperm injection [ICSI]), embryo biopsy for preimplantation genetic testing for aneuploidies or for monogenic/single-gene defects (PGT-A or PGT-M) programs, assisted hatching (AH), oocytes donation, female age, and embryo cryopreservation. Result(s): A total of 40 studies were included. Blastocyst transfer compared with cleavage-stage embryo transfer, and female age <35 years were associated with a statistically significant increase in the MZT and MCT pregnancy rate after IVF: (23 studies, OR 2.16, 95% CI, 1.74\u20132.68, I 2 =78%; 4 studies, OR 1.29; 95% CI, 1.03\u20131.62, I 2 =62%; and 3 studies, OR 1.90, 95% CI, 1.21\u20132.98, I 2 =59%; 2 studies, OR 2.34; 95% CI, 1.69\u20133.23, I 2 =0, respectively). Conventional IVF compared with ICSI and assisted hatching were associated with a statistically significantly increased risk of MZT pregnancy (9 studies, OR 1.19, 95% CI, 1.04\u20131.35, I 2 =0; 16 studies, OR 1.17, 95% CI, 1.09\u20131.27, I 2 =29%, respectively). Embryo biopsy for PGT-A or PGT-M, embryo cryopreservation, and oocytes donation were not associated with MZT pregnancies after IVF. Conclusion(s): Blastocyst transfer is associated with an increased risk of both MZT and MCT pregnancies after IVF. Further evidence is needed to clarify the impact of female age, insemination method and AH on the investigated outcomes

Oocyte retrieval difficulties in women with ovarian endometriomas

Research question: What are the frequency, characteristics and consequences of technical diffiiculties encountered by physicians when carrying out oocyte retrieval in women with ovarian endometriomas? Design: We prospectively recruited women undergoing IVF and compared technical difficulties between women with (n = 56) and without (n = 227) endometriomas. Results: In exposed women, the cyst had to be transfixed in eight cases (14%, 95% CI 7 to 25%) and accidental contamination of the follicular fluid with the endometrioma content was recorded in nine women (16%, 95% CI 8 to 27%). Moreover, follicular aspiration was more frequently incomplete (OR 3.6, 95% CI 1.4 to 9.6). In contrast, the retrievals were not deemed to be more technically difficult by the physicians and the rate of oocytes retrieved per developed follicle did not differ. No pelvic infections or cyst ruptures were recorded (0%, 95% CI 0 to 5%). Conclusions: Oocyte retrieval in women with ovarian endometriomas is more problematic but the magnitude of these increased difficulties is modest

Nutraceuticals and bioactive components from fish for dyslipidemia and cardiovascular risk reduction

Cardiovascular disease remains the most common health problem in developed countries, and residual risk after implementing all current therapies is still high. Permanent changes in lifestyle may be hard to achieve and people may not always be motivated enough to make the recommended modifications. Emerging research has explored the application of natural food-based strategies in disease management. In recent years, much focus has been placed on the beneficial effects of fish consumption. Many of the positive effects of fish consumption on dyslipidemia and heart diseases have been attributed to n-3 polyunsaturated fatty acids (n-3 PUFAs, i.e., EPA and DHA); however, fish is also an excellent source of protein and, recently, fish protein hydrolysates containing bioactive peptides have shown promising activities for the prevention/management of cardiovascular disease and associated health complications. The present review will focus on n-3 PUFAs and bioactive peptides effects on cardiovascular disease risk factors. Moreover, since considerable controversy exists regarding the association between n-3 PUFAs and major cardiovascular endpoints, we have also reviewed the main clinical trials supporting or not this association

Diritto Privato

Lo scritto ha ad oggetto i capitoli su modi di estinzione dell'obbligazione diversi dall'adempimento, singoli contratti, possesso, famiglia e trascrizione dei tomi secondo e terzo del manuale Diritto Privato (Utet) in adozione presso alcune Università Italiane e diffuso anche all’estero. I menzionati capitoli si segnalano per una trattazione non meramente istituzionale, ma particolarmente attenta al dato applicativo e giurisprudenziale, che rende il manuale stesso utilizzato largamente per la preparazione all’esame per avvocato e ai concorsi pubblici di secondo livello (magistratura ordinaria e amministrativa.

Effects of Vegetable Proteins on Hypercholesterolemia and Gut Microbiota Modulation

Risk assessment tools, i.e., validated risk prediction algorithms, to estimate the patient\u2019s 10-year risk of developing cardiovascular disease (CVD) should be used to identify high-risk people for primary prevention. Current evidence confirms that appropriate monitoring and control of risk factors either reduces the likelihood of CVD or slows down its progression. It is thus crucial that all health professionals make appropriate use of all the available intervention strategies to control risk factors: from dietary improvement and adequate physical activity to the use of functional foods, food supplements, and drugs. The gut microbiota, which encompasses 1 7 1014 resident microorganisms, has been recently recognized as a contributing factor in the development of human disease. This review examines the effect of both some vegetable food components belong to the \u201cprotein food group\u201d and the underexploited protein-rich hempseed on cholesterolemia and gut microbiota composition

Magnetic Resonance Imaging Visualization of Vulnerable Atherosclerotic Plaques at the Brachiocephalic Artery of Apolipoprotein E Knockout Mice by the Blood-pool Contrast Agent B22956/1

The aim of this study was to identify, by magnetic resonance imaging (MRI), the ability of the blood-pool contrast agent B22956/1 to detect atherosclerotic plaques developing at the brachiocephalic artery of apolipoprotein E knockout (apoE-KO) mice and to possibly identify vulnerable atherosclerotic lesions. After high-fat feeding for 8 or 12 weeks, MRIs of brachiocephalic arteries were acquired before and after B22956/1 administration; then vessels were removed and analyzed by histology. B22956/1 injection caused a rapid increase in plaque signal enhancement and plaque to muscle contrast values, which remained stable up to 70 minutes. A linear correlation between signal enhancement and macrophage content was found 10 minutes after B22956/1 injection ( p < .01). Signal enhancement and plaque to muscle contrast values correlated with macrophage content 40 minutes after contrast agent administration ( p < .01). Finally, 70 minutes after B22956/1 infusion, plaque to muscle contrast significantly correlated with the percentage of stenosis ( p < .005). B22956/1 administration to high fat-fed apoE-KO mice resulted in a rapid enhancement of atherosclerotic plaques and in a great ability to rapidly visualize vulnerable plaques, characterized by a high macrophage content. These results suggest that B22956/1 could represent an interesting tool for the identification of atherosclerotic plaques potentially leading to acute cardiovascular events

REMEDIACION DE RESIDUOS SÓLIDOS CONTAMINADOS CON Cr(VI) POR UN HONGO FILAMENTOSO

La biotecnología ambiental recurre a organismos capaces de reducir los niveles de metales pesados, entre ellos el Cr(VI), contenido en residuos y efluentes agroindustriales. El objetivo del trabajo fue estudiar la biorremediación de un residuo como pulpa de limón contaminada con Cr(VI) y el efecto del metal sobre el crecimiento fúngico. Se utilizaron tres hongos filamentosos como Aspergillus niger; Penicillium expansum y P. islandicum para remediar pulpa de limón (residuo) contaminada con Cr(VI) (50 mg/L) que se realizó en las siguientes condiciones de cultivo: la pulpa se suplementó con urea, 0,006; (NH4) 2SO4 , 0,012; KH2PO4 ,0,003 y KCl, 0,001 g/ g; 105 conidios/ g, a pH 2,5, 30°C y 96 h de incubación. Se estudió el efecto tóxico de diferentes concentraciones (5; 10; 20 y 50 mg/L) del metal sobre el desarrollo del hongo de mayor eficiencia de remediación (Ef.%). Aspergillus niger; obtuvo mayor EF. de remediación (97%) respecto a Penicillium expansum (95%) y P. islandicum (94%), del residuo contaminado con 50 mg/L de Cr(VI). Se determinó que la presencia de Cr(VI) y no su concentración estimuló la maduración temprana (48 h) de los conidios (blancos a negros) de A. niger, sin que se observe alteraciones en el micelio con respecto al control (72h), desarrollado en la pulpa sin el metal. En conclusión, A. niger fue más resistente y presentó altas Ef. de remediación de Cr(VI) de residuos sólidos, este proceso es una alternativa a las tecnologías físico-químicas, debido que los microorganismos pueden remover selectivamente diferentes iones de zonas contaminadas

Per scaldare, per cuocere e per produrre. Le strutture da fuoco dell&#8217;abitato etrusco del Forcello di Bagnolo S.Vito (MN) : aspetti tipologici e funzionali

Il Forcello di Bagnolo S.Vito (MN) \ue8 il principale abitato dell\u2019area di espansione etrusca a nord del Po nel VI e V secolo a.C. Si tratta di un insediamento esteso circa 12 ettari dalla struttura pienamente urbana: un impianto ortogonale caratterizzato da assi viari principali e strade minori che si intersecano ortogonalmente e individuano quartieri, occupati da edifici sia di tipo residenziale che produttivo. Le indagini archeologiche, estese su un\u2019area di circa 900 m2 nel nucleo centrale dell\u2019insediamento, si susseguono da oltre trent\u2019anni e hanno permesso di riconoscere nove fasi insediative, definite dallo stratificarsi di attivit\ue0 domestiche, attivit\ue0 artigianali ed eventi catastrofici. L\u2019estensione e la lunga durata delle ricerche hanno permesso di riportare alla luce decine di strutture da fuoco: focolari, forni a fossa per attivit\ue0 pirotecnologiche, resti di fornaci per la cottura della ceramica. L\u2019ampia variet\ue0 tipologica delle strutture da fuoco rinvenute \ue8 cos\uec associata alla funzione degli ambienti in cui esse si trovano, interpretata a sua volta grazie allo studio parallelo dei reperti associati e delle tecniche edilizie.The Forcello site (Bagnolo S. Vito, Mantua) is the main Etruscan settlement north of the Po in the 6th and 5th centuries BC. It is approximately 12-hectare wide, with a fully urban pattern. It\u2019s characterized by an orthogonal plan, defined by main and secondary roads that intersect each other orthogonally and outline blocks, occupied by both residential and productive buildings. The archaeological investigations are carried out in the settlement\u2019s core area, over about 900 m2. After more than thirty years of excavations, nine archaeological phases have been recognized, resulting by the stratification of domestic activities, craft activities and catastrophic events. The extension and the long duration of the researches have brought to light dozens of fire structures: hearths, pit furnaces to cast bronze or to forge iron objects, remains of kilns. The wide typological variety of the fire structures can be evaluated according to the function of the buildings in which they are located, detected by study of archaeological findings and building techniques