159 research outputs found
FAIMS operation for realistic gas flow profile and asymmetric waveforms including electronic noise and ripple
Large-scale analysis of peptide sequence variants : the case for high-field asymmetric waveform ion mobility spectrometry
[Image: see text] Large scale analysis of proteins by mass spectrometry is becoming increasingly routine; however, the presence of peptide isomers remains a significant challenge for both identification and quantitation in proteomics. Classes of isomers include sequence inversions, structural isomers, and localization variants. In many cases, liquid chromatography is inadequate for separation of peptide isomers. The resulting tandem mass spectra are composite, containing fragments from multiple precursor ions. The benefits of high-field asymmetric waveform ion mobility spectrometry (FAIMS) for proteomics have been demonstrated by a number of groups, but previously work has focused on extending proteome coverage generally. Here, we present a systematic study of the benefits of FAIMS for a key challenge in proteomics, that of peptide isomers. We have applied FAIMS to the analysis of a phosphopeptide library comprising the sequences GPSGXVpSXAQLX(K/R) and SXPFKXpSPLXFG(K/R), where X = ADEFGLSTVY. The library has defined limits enabling us to make valid conclusions regarding FAIMS performance. The library contains numerous sequence inversions and structural isomers. In addition, there are large numbers of theoretical localization variants, allowing false localization rates to be determined. The FAIMS approach is compared with reversed-phase liquid chromatography and strong cation exchange chromatography. The FAIMS approach identified 35% of the peptide library, whereas LC–MS/MS alone identified 8% and LC–MS/MS with strong cation exchange chromatography prefractionation identified 17.3% of the library
Separation and Identification of Isomeric Glycopeptides by High Field Asymmetric Waveform Ion Mobility Spectrometry
Measurements of and production in proton–proton interactions at in the NA61/SHINE experiment
International audienceThe production of and hyperons in inelastic p+p interactions is studied in a fixed target experiment at a beam momentum of 158 . Double differential distributions in rapidity and transverse momentum are obtained from a sample of 33M inelastic events. They allow to extrapolate the spectra to full phase space and to determine the mean multiplicity of both and . The rapidity and transverse momentum spectra are compared to transport model predictions. The mean multiplicity in inelastic p+p interactions at 158 is used to quantify the strangeness enhancement in A+A collisions at the same centre-of-mass energy per nucleon pair
Measurements of and production in proton–proton interactions at in the NA61/SHINE experiment
Double-differential yields of and
resonances produced in \pp interactions
were measured at a laboratory beam momentum of 158~\GeVc. This measurement is
the first of its kind in \pp interactions below LHC energies. It was performed
at the CERN SPS by the \NASixtyOne collaboration. Double-differential
distributions in rapidity and transverse momentum were obtained from a sample
of 2610 inelastic events. The spectra are extrapolated to full phase
space resulting in mean multiplicity of (6.73
0.25 0.67) and (2.71
0.18 0.18). The rapidity and transverse momentum
spectra and mean multiplicities were compared to predictions of string-hadronic
and statistical model calculations
Two-particle correlations in azimuthal angle and pseudorapidity in central collisions at the CERN Super Proton Synchrotron
- …