A simple low-SAR technique for chemical-shift selection with high-field spin-echo imaging

We have discovered a simple and highly robust method for removal of chemical shift artifact in spin-echo MR images, which simultaneously decreases the radiofrequency power deposition (specific absorption rate). The method is demonstrated in spin-echo echo-planar imaging brain images acquired at 7 T, with complete suppression of scalp fat signal. When excitation and refocusing pulses are sufficiently different in duration, and thus also different in the amplitude of their slice-select gradients, a spatial mismatch is produced between the fat slices excited and refocused, with no overlap. Because no additional radiofrequency pulse is used to suppress fat, the specific absorption rate is significantly reduced compared with conventional approaches. This enables greater volume coverage per unit time, well suited for functional and diffusion studies using spin-echo echo-planar imaging. Moreover, the method can be generally applied to any sequence involving slice-selective excitation and at least one slice-selective refocusing pulse at high magnetic field strengths. The method is more efficient than gradient reversal methods and more robust against inhomogeneities of the static (polarizing) field (B0)

A common theme in extracellular fluids of beetles: extracellular superoxide dismutases crucial for balancing ROS in response to microbial challenge

Extracellular Cu/Zn superoxide dismutases (SODs) are critical for balancing the level of reactive oxygen species in the extracellular matrix of eukaryotes. In the present study we have detected constitutive SOD activity in the haemolymph and defensive secretions of different leaf beetle species. Exemplarily, we have chosen the mustard leaf beetle, Phaedon cochleariae, as representative model organism to investigate the role of extracellular SODs in antimicrobial defence. Qualitative and quantitative proteome analyses resulted in the identification of two extracellular Cu/Zn SODs in the haemolymph and one in the defensive secretions of juvenile P. cochleariae. Furthermore, quantitative expression studies indicated fat body tissue and defensive glands as the main synthesis sites of these SODs. Silencing of the two SODs revealed one of them, PcSOD3.1, as the only relevant enzyme facilitating SOD activity in haemolymph and defensive secretions in vivo. Upon challenge with the entomopathogenic fungus, Metarhizium anisopliae, PcSOD3.1-deficient larvae exhibited a significantly higher mortality compared to other SOD-silenced groups. Hence, our results serve as a basis for further research on SOD regulated host-pathogen interactions. In defensive secretions PcSOD3.1-silencing affected neither deterrent production nor activity against fungal growth. Instead, we propose another antifungal mechanism based on MRJP/yellow proteins in the defensive exudates

Coulomb-Driven Cluster-Glass Behavior in Mn-Intercalated Ti1+yS2

We have investigated the low-temperature spin-glasslike phase in the intercalated transition-metal dichalcogenide Mn0.09Ti1.1S2. A departure from Curie–Weiss behavior in the paramagnetic regime indicated the formation of small ferromagnetically correlated clusters. The Vogel–Fulcher law provided an excellent description of relaxation times in the vicinity of the transition, showing that the glasslike phase occurs due to interaction between the clusters. Cole–Cole plots for data close to the transition were linear, which is consistent with a simple exponential distribution of cluster sizes. A Monte Carlo simulation of the dichalcogenide system, including excess self-intercalated Ti ions, gave an exponential cluster-size distribution for a relatively narrow range of concentration values of Mn and Ti ions, values that were consistent with those of the Mn0.09Ti1.1S2 sample. Strong commonality in the relaxation behavior with certain ferroelectric relaxor systems suggests underlying similarity in the microscopic structure of the clusters in both systems, which may be chainlike or quasi-one-dimensional

A 2D multiwavelength study of the ionized gas and stellar population in the Giant HII Region NGC 588

We present an analysis of NGC588 based on IFS data with PMAS, together with Spitzer images at 8 mi and 24 mi. The extinction distribution in the optical shows complex structure, with maxima correlating in position with those of the emission at 24 mi and 8 mi. The Ha luminosity absorbed by the dust within the GHIIR reproduces the structure observed in the 24 mi image, supporting the use of this band as a tracer of recent star formation. A velocity difference of ~50 km/s was measured between the areas of high and low surface brightness, which would be expected if NGC588 were an evolved GHIIR. Line ratios used in the BPT diagnostic diagrams show a larger range of variation in the low surface brightness areas. The ranges are ~0.5 to 1.2 dex for [NII]/Ha, 0.7 to 1.7 dex for [SII]/Ha, and 0.3 to 0.5 dex for [OIII]/Hb. Ratios corresponding to large ionization parameter (U) are found between the peak of the emission in Hb and the main ionizing source decreasing radially outwards within the region. Differences between the integrated and local values of the U tracers can be as high as ~0.8 dex. [OII]/Hb and [OIII]/[OII] yield similar local values for U and consistent with those expected from the integrated spectrum of an HII region ionized by a single star. The ratio [SII]/Ha departs significantly from the range predicted by this scenario, indicating the complex ionization structure in GHIIRs. There is a significant scatter in derivations of Z using strong line tracers as a function of position, caused by variations in the degree of ionization. The scatter is smaller for N2O3 which points to this tracer as a better Z tracer than N2. The comparison between integrated and local line ratio values indicates that measurements of the line ratios of GHIIR in galaxies at distances >~25 Mpc may be dominated by the ionization conditions in their low surface brightness areas.AM-I, EP-M and JMV acknowledge partial funding through research projects AYA2007-67965-C03-02 from the Spanish PNAYA and CSD2006-00070 1st Science with GTC of the MICINN. MR is supported by a Marie Curie Intra European Fellowship within the 7th European Community Framework Programme. CK, as a Humboldt Fellow, acknowledges support from the Alexander von Humboldt Foundation, Germany

gyrA mutations and phenotypic susceptibility levels to ofloxacin and moxifloxacin in clinical isolates of Mycobacterium tuberculosis

Objectives To compare mutations in the quinolone resistance-determining region of the gyrA gene and flanking sequences with the MICs of ofloxacin and moxifloxacin for Mycobacterium tuberculosis. Methods The presence of mutations in 177 drug-resistant M. tuberculosis isolates was determined by DNA sequencing and the MICs quantified by MGIT 960. Results Single nucleotide polymorphisms were detected at codons 94 (n = 30), 90 (n = 12), 91 (n = 3), 89 (n = 1), 88 (n = 1) and 80 (n = 1). Four isolates with double mutations D94G plus A90V (n = 2) and D94G plus D94N (n = 2) reflect mixed populations. Agreement between genotypic and phenotypic susceptibility was high (≥97%) for both drugs. Mutant isolates had an MIC50 of 8.0 mg/L and an MIC90 of >10 mg/L for ofloxacin compared with an MIC50 and MIC90 of 2.0 mg/L for moxifloxacin. Codons 94 and 88 were linked to higher levels of fluoroquinolone resistance compared with codons 90, 91 and 89. The MIC distributions for the wild-type isolates ranged from ≤0.5 to 2.0 mg/L for ofloxacin and from ≤0.125 to 0.25 mg/L for moxifloxacin. However, 96% of the isolates with genetic alterations had MICs ≤2.0 mg/L for moxifloxacin, which is within its achievable serum levels. Conclusions This study provides quantitative evidence that the addition of moxifloxacin to extensively drug-resistant tuberculosis (XDR-TB) regimens based on a clinical breakpoint of 2.0 mg/L has merit. The use of moxifloxacin in the treatment of multidrug-resistant tuberculosis may prevent the acquisition of additional mutations and development of XDR-T