709 research outputs found
Method and apparatus for detecting flaws and defects in heat seals
Flaws and defects in heat seals formed between sheets of translucent film are identified by optically examining consecutive lateral sections of the seal along the seal length. Each lateral seal section is illuminated and an optical sensor array detects the intensity of light transmitted through the seal section for the purpose of detecting and locating edges in the heat seal. A line profile for each consecutive seal section is derived having an amplitude proportional to the change in light intensity across the seal section. Instances in the derived line profile where the amplitude is greater than a threshold level indicate the detection of a seal edge. The detected edges in each derived line profile are then compared to a preset profile edge standard to identify the existence of a flaw or defect
Application of CE-MS to examination of black inkjet printing inks for forensic purposes.
The potential of capillary electrophoresis coupled to mass spectrometry with electrospray ion source and time of flight analyser (CE-ESI-TOF-MS) in the analysis of inkjet inks was investigated. The developed and validated method allowed reliable and repeatable analysis of black inkjet inks extracted from printouts. Over a dozen inkjet printouts printed on various printer models from different manufacturers were analysed under selected conditions to determine the variation of chemical composition of inks between different brands and types. It was ascertained that the developed method is capable of revealing qualitative differences between ink samples. For most of the investigated inks, the studies showed the presence of a characteristic mass spectrum originating from the surfactant or polymer. The mass distribution of the additive is distinctive for some inkjet ink producers, and allows for group identification of inks. The results showed the strength of the CE-ESI-TOF-MS method as an effective technique for forensic purposes, requiring a small amount of inkjet ink samples and giving analytical information that is useful in the identification of compounds
Exploitation of the coil-globule plasmid DNA transition induced by small changes in temperature, pH salt, and poly(ethylene glycol) compositions for directed partitioning in aqueous two-phase systems
In this study, the interplay of two linked equilibria is examined, one concerning an aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and salt employed to partition plasmid DNA (pDNA), and the other a potential structural transition of pDNA depending on PEG and salt concentration and other system parameters. The boundary conditions for pDNA partitioning are set by PEG and salt concentrations, PEG molecular weight, pH, and temperature. While investigating these parameters, it was found that a small increase/decrease of the respective values led to a drastic and significant change in pDNA behavior. This behavior could be attributed to a coil-globule transition of the pDNA triggered by the respective phase conditions. The combination of this structural change, aggregation effects linked to the transition process, and the electrostatic potential difference found in PEG-salt systems thus offers a sensitive way to separate nucleic acid forms on the basis of their unique property to undergo coil-globule transitions under distinct system properties
Exploring Strategies To Promote Engagement And Active Learning Through Digital Course Design In Engineering Mathematics
This research explores the strategies and techniques used to foster and promote the engagement and active learning of engineering students within a digital course. This digital course has been developed to address the varying levels of understanding of fundamental mathematics among first-year engineering students, who often have disparate levels of prior knowledge at their high school completion. We observe an increasing need to bridge the widening gap between high school and university mathematics in order to prevent engineering students from being hindered in their academic successes due to a lack of prior mathematical understanding. With a team of engineers and mathematicians, both researchers and educators, we are developing a mathematics Bridging Course including the use of digital tools, such as videos, online interactions and technology-based assessments. These sources were created, investigated and/or modified to develop an engaging learning environment in which students are made aware of and guided through misconceptions and mistakes in their understanding of fundamental mathematics. In the development of this Bridging Course, we consider the importance of interactive learning and timely feedback for student learning. We investigate the impact of digital course design on students’ performance and learning outcomes using a qualitative approach. Students feedback within the first stage of the implementation of the course offered a positive assessment of the course, accentuating its inherent advantages and attributes. The students’ feedback proved to be an invaluable source of insights, specifically concerning the enhancement of question distractors, thus prompting revisions and augmentations in the assessment items employed
The influence of cell adsorbent interactions on protein adsorption in expanded beds
Expanded bed adsorption (EBA) is a primary recovery operation allowing the adsorption of proteins directly from unclarified feedstock, e.g. culture suspensions, homogenates or crude extracts. Thus solid-liquid separation is combined with adsorptive purification in a single step. The concept of integration requires that the solid components of the feed solution are regarded as a part of the process, which influences stability, reproducibility, and overall performance. This aspect is investigated here at the example of the influence of presence and concentration of intact yeast cells (S. cerevisiae) on the adsorption of model proteins (hen egg white lysozyme and bovine serum albumin) to various stationary phases (cation and anion-exchange, hydrophobic interaction, immobilised metal affinity). The interaction of the cells with the adsorbents is determined qualitatively and quantitatively by a pulse response method as well as by a finite bath technique under different operating conditions. The consequence of these interactions for the stability of expanded beds in suspensions of varying cell concentration is measured by residence time distributions (RTDs) after tracer pulse injection (NaBr, LiCl). Analysis of the measured RTD by the PDE model allows the calculation of the fraction of perfectly fluidised bed (φ), a parameter which may be regarded as a critical quantity for the estimation of the quality of fluidisation of adsorbents in cell containing suspensions. The correlation between bed stability and performance is made by analysing the breakthrough of model proteins during adsorption from unclarified yeast culture broth. A clear relationship is found between the degree of cell/adsorbent interaction, bed stability in terms of the φ parameter, and the sorption efficiency. Only beds characterised by a φ; value larger than 0.8 in the presence of cells will show a conserved performance compared to adsorption from cell free solutions. A drop in φ, which is due to interactions of the fluidised adsorbent particles with cells from the feed, will directly result in a reduced breakthrough efficiency. The data presented highlight the importance of including the potential interaction of solid feedstock components and the expanded adsorbents into the design of EBA processes, as the interrelation found here is a key factor for the overall performance of EBA as a truly integrated operation. © 2000 Elsevier Science B.V.Fil: Fernandez Lahore, Hector. Universitat Dusseldorf; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Geilenkirchen, S.. Universitat Dusseldorf; AlemaniaFil: Boldt, K.. Universitat Dusseldorf; AlemaniaFil: Nagel, A.. Universitat Dusseldorf; AlemaniaFil: Kula, M. R.. Universitat Dusseldorf; AlemaniaFil: Thömmes, J.. Universitat Dusseldorf; Alemani
Three and four current reversals versus temperature in correlation ratchets with a simple sawtooh potential
Transport of Brownian particles on a simple sawtooth potential subjected to
both unbiased thermal and nonequilibrium symmetric three-level Markovian noise
is considered. The new effects of three and four current reversals as a
function of temperature are established in such correlation ratchets. The
parameter space coordinates of the fixed points associated with these current
reversals and the necessary and sufficient conditions for the existence of the
novel current reversals are found.Comment: 4 pages, 5 figures; some changes introduced; accepted for publication
in Physical Review
Intraoperative effectiveness of crystalloid and colloid volume substitution in patients undergoing elective major urological surgery by maintenance of the cardiac index within normal range
Priority resource access mediates competitive intensity between an invasive weevil and native floral herbivores
Mechanisms underlying invasive species impacts remain incompletely understood. We tested the hypothesis that priority resource access by an invasive biocontrol weevil, Rhinocyllus conicus, intensifies and alters the outcome of competition with native floral herbivores over flower head resources of the non-target, native host plant Cirsium canescens, specifically with the predominant, synchronous tephritid fly Paracantha culta. Four main results emerged. First, we documented strong, asymmetric competition, with R. conicus out-competing P. culta. Second, weevil priority access to floral resources accelerated competitive suppression of P. culta. Evidence for competitive suppression with increased weevil priority included decreases in both the numbers and the total biomass of native flies, plus decreases in individual P. culta fly mass and, so, potential fitness. Third, we found evidence for three concurrent mechanisms underlying the competitive suppression of P. culta by R. conicus. Prior use of a flower head by R. conicus interfered with P. culta pre-oviposition behavior. Once oviposition occurred, the weevil also reduced fly post-oviposition performance. Preemptive resource exploitation occurred, shown by the significant effect of flower head size on the total number of insects developing and in the magnitude of R. conicus effects on P. culta. Interference also occurred, shown by a spatial shift of surviving P. culta individuals away from the preferred receptacle resources as R. conicus priority increased. Finally, fourth, using an individual-based model (IBM), we found that the competitive interactions documented have the potential for imposing demographic consequences, causing a reduction in P. culta population sizes. Thus, priority resource access by an invasive insect increased competitive impact on the predominant native insect in the invaded floral guild. This study also provides the first experimental evidence for non-target effects of a weed biological control agent on an associated native insect herbivore.
Includes supplementary materials
Irreversible and reversible modes of operation of deterministic ratchets
We discuss a problem of optimization of the energetic efficiency of a simple
rocked ratchet. We concentrate on a low-temperature case in which the
particle's motion in a ratchet potential is deterministic. We show that the
energetic efficiency of a ratchet working adiabatically is bounded from above
by a value depending on the form of ratchet potential. The ratchets with
strongly asymmetric potentials can achieve ideal efficiency of unity without
approaching reversibility. On the other hand we show that for any form of the
ratchet potential a set of time-protocols of the outer force exist under which
the operation is reversible and the ideal value of efficiency is also achieved.
The mode of operation of the ratchet is still quasistatic but not adiabatic.
The high values of efficiency can be preserved even under elevated
temperatures
- …