10,839 research outputs found
Purification and biochemical characterization of a serine alkaline protease TC4 from a new isolated Bacillus alcalophilus TCCC11004 in detergent formulations
An extracellular alkaline protease producing strain was isolated from alkaline soil and identified as Bacillus alcalophilus TCCC11004 on the basis of 16S rDNA gene sequencing and biochemical properties. The most appropriate medium for the protease production was composed of (g/l): maltodextrin 110, yeast extract 17.5, cotton seed meal 29.3, K2HPO4 18, trisodium citrate 3.3 and CaCl2 2.6. The alkaline protease TC4 was purified from the culture supernatant by ammonium sulfate precipitation, Sephadex G-75 gel filtration and SP-Sepharose HP ion exchange chromatography, with a 6.8 fold increase in specific activity and 15.2% recovery. The molecular weight was estimated to be 26 kDa on SDS-PAGE. The protease was highly active from pH 9.0-12.0 with an optimal at pH 11.0. It was active at 30 - 60°C and exhibited maximal activity at 50°C. The thermostability of the protease was increased by the addition of CaCl2. It retained 70 and 81% of its initial activity after heating for 2 h at 50°C, in the absence or presence of 2 mM CaCl2, respectively. The enzyme was inactivated by diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride, suggesting that it is a serine protease. The protease was stable in 0.5% SDS and retained 70.3% of its initial activity after 1 h of incubation. It was active in the presence of 3% Triton X-100 with 100% activity and stable towards oxidizing agent with 69.2% activity in the presence of 1% H2O2. The enzyme showed excellent compatibility with commercial detergents such as TaiZi, BiLang, DiaoPai and TianQing, retaining more than 90% of its initial activity in the tested detergents after 1 h of preincubation at 40°C.Keywords: Serine alkaline protease, Bacillus alcalophilus, stability, detergent compatibility
Extreme objects with arbitrary large mass, or density, and arbitrary size
We consider a generalization of the interior Schwarzschild solution that we
match to the exterior one to build global C^1 models that can have arbitrary
large mass, or density, with arbitrary size. This is possible because of a new
insight into the problem of localizing the center of symmetry of the models and
the use of principal transformations to understand the structure of space.Comment: 20 pages, 6 figures. Fixed one reference. Added a new equatio
Comparing metrics at large: harmonic vs quo-harmonic coordinates
To compare two space-times on large domains, and in particular the global
structure of their manifolds, requires using identical frames of reference and
associated coordinate conditions. In this paper we use and compare two classes
of time-like congruences and corresponding adapted coordinates: the harmonic
and quo-harmonic classes. Besides the intrinsic definition and some of their
intrinsic properties and differences we consider with some detail their
differences at the level of the linearized approximation of the field
equations. The hard part of this paper is an explicit and general determination
of the harmonic and quo-harmonic coordinates adapted to the stationary
character of three well-know metrics, Schwarzschild's, Curzon's and Kerr's, to
order five of their asymptotic expansions. It also contains some relevant
remarks on such problems as defining the multipoles of vacuum solutions or
matching interior and exterior solutions.Comment: 27 pages, no figure
Minimal set of generators of controllability space for singular linear dynamical systems
Due to the significant role played by singular systems in the form E Âż x ( t ) = Ax ( t ) , on mathematical modeling of science and engineering problems; in the last years recent years its interest in the descriptive analysis of its structural and dynamic properties. However, much less effort has been devoted to studying the exact con- trollability by measuring the minimum set of controls needed to direct the entire system E Âż x ( t ) = Ax ( t ) to any desired state. In this work, we focus the study on obtaining the set of all matrices B with a minimal number of columns, by making the singular system E Âż x ( t ) = Ax ( t ) + Bu ( t ) controllable.Postprint (author's final draft
AKR7A3 suppresses tumorigenicity and chemoresistance in hepatocellular carcinoma through attenuation of ERK, c-Jun and NF-ÎşB signaling pathways
published_or_final_versio
Radiative decays of heavy and light mesons in a quark triangle approach
The radiative meson decays and are
analyzed using the quark triangle diagram. Experimental data yield well
determined estimates of the universal quark-antiquark-meson couplings
and for the light meson sector. Also
predictions for the ratios of neutral to charged heavy meson decay coupling
constants are given and await experimental confirmation.Comment: 31 pages of RevTex, 5 figures, Postscript version available at
http://info.utas.edu.au/docs/physics/theory/Publications/9548.html, scheduled
to appear in Phys. Rev. D, vol 53, issue 11, 199
Effects of Huanglian-Jie-Du-Tang and its modified formula on the modulation of amyloid-β precursor protein processing in Alzheimer's disease models.
published_or_final_versio
Prediction of preterm birth with and without preeclampsia using mid-pregnancy immune and growth-related molecular factors and maternal characteristics.
OBJECTIVE:To evaluate if mid-pregnancy immune and growth-related molecular factors predict preterm birth (PTB) with and without (±) preeclampsia. STUDY DESIGN:Included were 400 women with singleton deliveries in California in 2009-2010 (200 PTB and 200 term) divided into training and testing samples at a 2:1 ratio. Sixty-three markers were tested in 15-20 serum samples using multiplex technology. Linear discriminate analysis was used to create a discriminate function. Model performance was assessed using area under the receiver operating characteristic curve (AUC). RESULTS:Twenty-five serum biomarkers along with maternal age <34 years and poverty status identified >80% of women with PTB ± preeclampsia with best performance in women with preterm preeclampsia (AUC = 0.889, 95% confidence interval (0.822-0.959) training; 0.883 (0.804-0.963) testing). CONCLUSION:Together with maternal age and poverty status, mid-pregnancy immune and growth factors reliably identified most women who went on to have a PTB ± preeclampsia
Sequential decoupling of negative-energy states in Douglas-Kroll-Hess theory
Here, we review the historical development, current status, and prospects of
Douglas--Kroll--Hess theory as a quantum chemical relativistic electrons-only
theory.Comment: 15 page
Clades and clans: a comparison study of two evolutionary models
The Yule-Harding-Kingman (YHK) model and the proportional to distinguishable
arrangements (PDA) model are two binary tree generating models that are widely
used in evolutionary biology. Understanding the distributions of clade sizes
under these two models provides valuable insights into macro-evolutionary
processes, and is important in hypothesis testing and Bayesian analyses in
phylogenetics. Here we show that these distributions are log-convex, which
implies that very large clades or very small clades are more likely to occur
under these two models. Moreover, we prove that there exists a critical value
for each such that for a given clade with size ,
the probability that this clade is contained in a random tree with leaves
generated under the YHK model is higher than that under the PDA model if
, and lower if . Finally, we extend our results
to binary unrooted trees, and obtain similar results for the distributions of
clan sizes.Comment: 21page
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