Engaging with advertising media in a constructivist classroom : case study of a rural seventh grade class

Title from PDF of title page (University of Missouri--Columbia, viewed on May 31, 2012).The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.Dissertation advisor: Dr. Amy A. LanninVita.Ph. D. University of Missouri--Columbia 2011."December 2011"This study examined the experience of rural seventh grade students in a communication arts class where advertising media was the focus, and how these young adults talked, thought, and wrote about advertising media. Data was collected over four weeks during a unit on persuasive techniques in propaganda texts and included interviews, whole class and small group discussions, student writing, and student production of multimedia commercials and print ads. This qualitative case study provides an analysis of the instructional methods chosen by a constructivist teacher who engaged her students in the spring of the year, immersing them in the genre of advertisements, providing scaffolds and equipment for student use, and scheduling large blocks of time for students to talk, write, and create. The study provides insight into pedagogy and issues around media literacy.Includes bibliographical reference

Mission to a comet - Preliminary scientific objectives and experiments for use in advanced mission studies

Scientific objectives and experiments for comet missio

Porcine Cysticercosis in Southeast Uganda: Seroprevalence in Kamuli and Kaliro Districts

The recent recognition of neurocysticercosis as a major cause of epilepsy in Uganda and changes in pig demography have lead to a need to better understand the basic epidemiology of Taenia solium infections in pigs and humans. Human exposure is a function of the size of the animal reservoir of this zoonosis. This is the first field survey for porcine cysticercosis to investigate the prevalence of antigen-positive pigs across an entire rural district of south-east Uganda. In our field surveys, 8.6% of 480 pigs screened were seropositive for the parasite by B158/B60 Ag-ELISA. In addition, of the 528 homesteads surveyed 138 (26%) did not have pit latrines indicating a high probability of pigs having access to human faeces and thus T. solium eggs. This study thus indicates the need for better data on this neglected zoonotic disease in Uganda, with a particular emphasis on the risk factors for infection in both pigs and humans. In this regard, further surveys of pigs, seroprevalence surveys in humans and an understanding of cysticercosis-related epilepsy are required, together with risk-factor studies for human and porcine infections

A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins

We have devised a strategy based on polymerase chain reaction (PCR) for the rapid cloning of functional antibody genes as single-chain immunotoxins. RNA from a hybridoma producing an antibody (OVB3) that reacts with ovarian cancer cells was used as a template to make the first strand of a cDNA. Then a second strand was synthesized and amplified by using two sets of DNA primers that (i) hybridized to the ends of the light- and heavy-chain variable regions, (ii) encoded a linker peptide, and (iii) contained appropriate restriction enzyme sites for cloning. After 30 cycles of PCR, the DNA fragments containing sequences encoding the light- and heavy-chain variable regions were cloned into an Escherichia coli expression vector containing a portion of the Pseudomonas exotoxin gene. Clones encoding recombinant single-chain immunotoxins were expressed in E. coli and the protein product was assessed for its ability to bind to or kill cells bearing the OVB3 antigen. By using this approach it should be possible to rapidly clone the functional variable region sequences of many different antibodies from hybridoma RNA