Modification of light utilization for skeletal growth by water flow in the scleractinian coral Galaxea fascicularis

In this study, we tested the hypothesis that the importance of water flow for skeletal growth (rate) becomes higher with increasing irradiance levels (i.e. a synergistic effect) and that such effect is mediated by a water flow modulated effect on net photosynthesis. Four series of nine nubbins of G. fascicularis were grown at either high (600 µE m-2 s-1) or intermediate (300 µE m-2 s-1) irradiance in combination with either high (15–25 cm s-1) or low (5–10 cm s-1) flow. Growth was measured as buoyant weight and surface area. Photosynthetic rates were measured at each coral’s specific experimental irradiance and flow speed. Additionally, the instantaneous effect of water flow on net photosynthetic rate was determined in short-term incubations in a respirometric flowcell. A significant interaction was found between irradiance and water flow for the increase in buoyant weight, the increase in surface area, and specific skeletal growth rate, indicating that flow velocity becomes more important for coral growth with increasing irradiance levels. Enhancement of coral growth with increasing water flow can be explained by increased net photosynthetic rates. Additionally, the need for costly photo-protective mechanisms at low flow regimes could explain the differences in growth with flow

Phase toxicity of dodecane on the microalga Dunaliella salina

In the so-called milking process of Dunaliella salina carotenoids are extracted and simultaneously produced by the culture, whilst the biomass concentration remains constant. Different theories exist about the extraction mechanisms although none have been proven yet. In this research, direct contact between dodecane and cells during the extraction process was studied microscopically and effects of direct contact were determined during in situ extraction experiments. Our results showed that water– solvent interphase contact resulted in cell death. This cell death and consequent cell rupture resulted in the release and concomitant extraction of the carotenoids. Furthermore, it has been suggested to add a small amount of dichloromethane to the biocompatible dodecane to create an organic phase with more extraction capacity. Our results showed that the addition of dichloromethane resulted in increased cell death and consequently the extraction rate increased. The improved solubility of carotenoids in an organic phase with dichloromethane did not significantly increase the extraction rate

Light intensity, photoperiod duration, daily light flux and coral growth of Galaxea fascicularis in an aquarium setting: a matter of photons?

Light is one of the most important abiotic factors influencing the (skeletal) growth of scleractinian corals. Light stimulates coral growth by the process of light-enhanced calcification, which is mediated by zooxanthellar photosynthesis. However, the quantity of light that is available for daily coral growth is not only determined by light intensity (i.e. irradiance), but also by photoperiod (i.e. the light duration time). Understanding and optimizing conditions for coral growth is essential for sustainable coral aquaculture. Therefore, in this study, the question was explored whether more light (i.e. more photons), presented either as irradiance or as light duration, would result in more growth. A series of nine genetically identical coral colonies of Galaxea fascicularis L. were cultured for a period of 18 weeks at different light duration times (8 hours 150 µE m-2 s-1:16 hours dark, 12 hours 150 µE m-2 s-1:12 hours dark, 16 hours 150 µE m-2 s-1:8 hours dark, 24 hours 150 µE m-2 s-1:0 hours dark) and different irradiance levels (8 hours 150 µE m-2 s-1:16 hours dark, 8 hours 225 µE m-2 s-1:16 hours dark and 8 hours 300 µE m-2 s-1:16 hours dark). Growth was determined every two weeks by measuring buoyant weight. Temperature, salinity and feeding levels were kept constant during the experiment. To detect possible acclimation of the corals to an increased light duration, rates of net photosynthesis and dark respiration were measured, hereby comparing coral colonies grown under an 8:16 hours light (150 µE m-2 s-1):dark cycle with corals grown under a 16:8 hours light (150 µE m-2 s-1):dark cycle. No increase in growth was detected with either increasing photoperiod or irradiance. Continuous lighting (24 hours 150 µE m-2 s-1:0 hours dark) resulted in immediate bleaching and the corals died after 14 weeks. Hourly photosynthetic rates were significantly reduced in the 16 hour light treatment compared to the 8 hour light treatment. As a result, daily net photosynthetic rates were not significantly different, which may explain the observed specific growth rates. Acclimation to photoperiod duration appeared neither to be mediated by changes in chlorophyll-a concentration nor zooxanthellae density. Based on the results of this study, we can conclude that the enhancing effect of light on coral growth is not only a matter of photons. Obviously, the availability of light was not limiting growth in these experiments and was probably in excess (i.e. stressful amounts). Other factors are discussed that play a role in determining growth rates and might explain our results

Clinical DRUJ instability does not influence the long-term functional outcome of conservatively treated distal radius fractures

Trauma Surger

Overzicht van organisaties die zich bezighouden met (toepassing van) algenteelt

Dit rapport heeft tot doel een overzicht te geven van de voor Nederland belangrijkste activiteiten en bedrijfsplannen betreffende algen die nationaal en internationaal gaande zijn c.q. gemaakt worden. Er wordt een overzicht gegeven van organisaties die zich bezig houden met (toepassing van) algenteelt, onderverdeeld in Nederlandse (of in Nederland opererende) en buitenlandse organisaties. Er is ook een overzicht van projecten met Nederlandse inbreng op het gebied van (toepassing van) algenteelt. De projecten zijn gerangschikt in lopende grote, middelgrote, en kleine projecten, en in het oog springende initiatieven om tot een project te komen. Tot slot worden een paar algemene conclusies en aanbevelingen gegeven voor de korte en lange termijn

Marine Biotechnology: A New Vision and Strategy for Europe

Marine Board-ESF The Marine Board provides a pan-European platform for its member organisations to develop common priorities, to advance marine research, and to bridge the gap between science and policy in order to meet future marine science challenges and opportunities. The Marine Board was established in 1995 to facilitate enhanced cooperation between European marine science organisations (both research institutes and research funding agencies) towards the development of a common vision on the research priorities and strategies for marine science in Europe. In 2010, the Marine Board represents 30 Member Organisations from 19 countries. The Marine Board provides the essential components for transferring knowledge for leadership in marine research in Europe. Adopting a strategic role, the Marine Board serves its Member Organisations by providing a forum within which marine research policy advice to national agencies and to the European Commission is developed, with the objective of promoting the establishment of the European Marine Research Area

Dynamics of triacylglycerol and EPA production in Phaeodactylum tricornutum under nitrogen starvation at different light intensities

publishedVersio

Revisiting the seasonal cycle of the Timor throughflow: impacts of winds, waves and eddies

© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Peña‐Molino, B., Sloyan, B., Nikurashin, M., Richet, O., & Wijffels, S. Revisiting the seasonal cycle of the Timor throughflow: impacts of winds, waves and eddies. Journal of Geophysical Research: Oceans, 127, (2022): e2021JC018133, https://doi.org/10.1029/2021jc018133.The tropical Pacific and Indian Oceans are connected via a complex system of currents known as the Indonesian Throughflow (ITF). More than 30% of the variability in the ITF is linked to the seasonal cycle, influenced by the Monsoon winds. Despite previous efforts, a detailed knowledge of the ITF response to the components of the seasonal forcing is still lacking. Here, we describe the seasonal cycle of the ITF based on new observations of velocity and properties in Timor Passage, satellite altimetry and a high-resolution regional model. These new observations reveal a complex mean and seasonally varying flow field. The amplitude of the seasonal cycle in volume transport is approximately 6 Sv. The timing of the seasonal cycle, with semi-annual maxima (minima) in May and December (February and September), is controlled by the flow below 600 m associated with semi-annual Kelvin waves. The transport of thermocline waters (<300 m) is less variable than the deep flow but larger in magnitude. This top layer is modulated remotely by cycles of divergence in the Banda Sea, and locally through Ekman transport, coastal upwelling, and non-linearities of the flow. The latter manifests through the formation of eddies that reduce the throughflow during the Southeast Monsoon, when is expected to be maximum. While the reduction in transport associated with the eddies is small, its impact on heat transport is large. These non-linear dynamics develop over small scales (<10 km), and without high enough resolution, both observations and models will fail to capture them adequately.B. Peña-Molino, B. M. Sloyan, M. Nikurashin, and O. Richet were supported by the Centre for Southern Hemisphere Oceans Research (CSHOR). CSHOR is a joint research Centre for Southern Hemisphere Ocean Research between QNLM and CSIRO. S. E. Wijffels was supported by the US National Science Foundation Grant No. OCE-1851333

Voltage analysis after multi-electrode ablation with duty-cycled bipolar and unipolar radiofrequency energy: a case report

Pulmonary vein ablation with a single-tip catheter remains long and complex. We describe a typical case of a novel efficient technique with a decapolar ring catheter utilizing alternating unipolar/bipolar radiofrequency energy. Voltage analysis and electrical mapping demonstrate the potential for antrum ablation and pulmonary vein isolation