Axisymmetric slosh frequencies of a liquid mass in a circular cylinder

Spectral eigenvalue methods along with some lower-dimensional techniques are used to determine the natural frequencies of a liquid slug in a circular tube. The contact lines are either pinned or governed by a slip coefficient assumed small. Corresponding physical experiments are conducted for a borosilicate glass tube and a treated water slug. Gravitational and viscous effects are neglected for the analyses. The spectral results agree well with a simple spherical end cap approximation (zero dimensional) for large aspect ratio slugs and with a membrane approximation (one dimensional) for small aspect ratios. The experimental observations for different aspect ratios agree well with the predictions, although the gravity, viscosity and/or slip are neglected in the analyses. © 2003 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/71120/2/PHFLE6-15-12-3659-1.pd

Estimation of Parameters in DNA Mixture Analysis

In Cowell et al. (2007), a Bayesian network for analysis of mixed traces of DNA was presented using gamma distributions for modelling peak sizes in the electropherogram. It was demonstrated that the analysis was sensitive to the choice of a variance factor and hence this should be adapted to any new trace analysed. In the present paper we discuss how the variance parameter can be estimated by maximum likelihood to achieve this. The unknown proportions of DNA from each contributor can similarly be estimated by maximum likelihood jointly with the variance parameter. Furthermore we discuss how to incorporate prior knowledge about the parameters in a Bayesian analysis. The proposed estimation methods are illustrated through a few examples of applications for calculating evidential value in casework and for mixture deconvolution

Electronic dielectric constants of insulators by the polarization method

We discuss a non-perturbative, technically straightforward, easy-to-use, and computationally affordable method, based on polarization theory, for the calculation of the electronic dielectric constant of insulating solids at the first principles level. We apply the method to GaAs, AlAs, InN, SiC, ZnO, GaN, AlN, BeO, LiF, PbTiO$_3$, and CaTiO$_3$. The predicted \einf's agree well with those given by Density Functional Perturbation Theory (the reference theoretical treatment), and they are generally within less than 10 % of experiment.Comment: RevTeX 4 pages, 2 ps figure

Amplitude measurements of Faraday waves

A light reflection technique is used to measure quantitatively the surface elevation of Faraday waves. The performed measurements cover a wide parameter range of driving frequencies and sample viscosities. In the capillary wave regime the bifurcation diagrams exhibit a frequency independent scaling proportional to the wavelength. We also provide numerical simulations of the full Navier-Stokes equations, which are in quantitative agreement up to supercritical drive amplitudes of 20%. The validity of an existing perturbation analysis is found to be limited to 2.5% overcriticaly.Comment: 7 figure

Energy transport by nonlinear internal waves

Winter stratification on Oregon’s continental shelf often produces a near-bottom layer of dense fluid that acts as an internal waveguide upon which nonlinear internal waves propagate. Shipboard profiling and bottom lander observations capture disturbances that exhibit properties of internal solitary waves, bores, and gravity currents. Wavelike pulses are highly turbulent (instantaneous bed stresses are 1 N m−2), resuspending bottom sediments into the water column and raising them 30+ m above the seafloor. The wave cross-shelf transport of fluid often counters the time-averaged Ekman transport in the bottom boundary layer. In the nonlinear internal waves that were observed, the kinetic energy is roughly equal to the available potential energy and is O(0.1) megajoules per meter of coastline. The energy transported by these waves includes a nonlinear advection term 〈uE〉 that is negligible in linear internal waves. Unlike linear internal waves, the pressure–velocity energy flux 〈up〉 includes important contributions from nonhydrostatic effects and surface displacement. It is found that, statistically, 〈uE〉 ≃ 2〈up〉. Vertical profiles through these waves of elevation indicate that up(z) is more important in transporting energy near the seafloor while uE(z) dominates farther from the bottom. With the wave speed c estimated from weakly nonlinear wave theory, it is verified experimentally that the total energy transported by the waves is 〈up〉 + 〈uE〉 ≃ c〈E〉. The high but intermittent energy flux by the waves is, in an averaged sense, O(100) watts per meter of coastline. This is similar to independent estimates of the shoreward energy flux in the semidiurnal internal tide at the shelf break

Microscopic modelling of doped manganites

Colossal magneto-resistance manganites are characterised by a complex interplay of charge, spin, orbital and lattice degrees of freedom. Formulating microscopic models for these compounds aims at meeting to conflicting objectives: sufficient simplification without excessive restrictions on the phase space. We give a detailed introduction to the electronic structure of manganites and derive a microscopic model for their low energy physics. Focussing on short range electron-lattice and spin-orbital correlations we supplement the modelling with numerical simulations.Comment: 20 pages, 10 figs, accepted for publ. in New J. Phys., Focus issue on Orbital Physic

Aspergillus fumigatus and aspergillosis: From basics to clinics

The airborne fungus Aspergillus fumigatus poses a serious health threat to humans by causing numerous invasive infections and a notable mortality in humans, especially in immunocompromised patients. Mould-active azoles are the frontline therapeutics employed to treat aspergillosis. The global emergence of azole-resistant A. fumigatus isolates in clinic and environment, however, notoriously limits the therapeutic options of mould-active antifungals and potentially can be attributed to a mortality rate reaching up to 100 %. Although specific mutations in CYP51A are the main cause of azole resistance, there is a new wave of azole-resistant isolates with wild-type CYP51A genotype challenging the efficacy of the current diagnostic tools. Therefore, applications of whole-genome sequencing are increasingly gaining popularity to overcome such challenges. Prominent echinocandin tolerance, as well as liver and kidney toxicity posed by amphotericin B, necessitate a continuous quest for novel antifungal drugs to combat emerging azole-resistant A. fumigatus isolates. Animal models and the tools used for genetic engineering require further refinement to facilitate a better understanding about the resistance mechanisms, virulence, and immune reactions orchestrated against A. fumigatus. This review paper comprehensively discusses the current clinical challenges caused by A. fumigatus and provides insights on how to address them.AA, RGR, and DSP were supported by NIH AI 109025. MH was supported by NIH UL1TR001442. AC was supported by the Fundação para a Ciência e a Tecnologia (FCT) (CEECIND/03628/2017 and PTDC/MED GEN/28778/2017). Additional support was provided by FCT (UIDB/50026/2020 and UIDP/50026/2020), the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) (NORTE-01-0145-FEDER-000013 and NORTE-01-0145-FEDER-000023), the European Union's Horizon 2020 Research and Innovation programme under grant agreement no. 847507, and the “la Caixa” Foundation (ID 100010434) and FCT under the agreement LCF/PR/HP17/52190003. DJA was supported by CF Trust Strategic Research Centre TrIFIC (SRC015), Wellcome Trust Collaborative Award 219551/Z/19/Z and the NIHR Centre for Antimicrobial Optimisation.S

Modeling the Atmospheric Boundary Layer Wind Response to Mesoscale Sea Surface Temperature Perturbations

The wind speed response to mesoscale SST variability is investigated over the Agulhas Return Current region of the Southern Ocean using the Weather Research and Forecasting (WRF) Model and the U.S. Navy Coupled Ocean–Atmosphere Mesoscale Prediction System (COAMPS) atmospheric model. The SST-induced wind response is assessed from eight simulations with different subgrid-scale vertical mixing parameterizations, validated using Quick Scatterometer (QuikSCAT) winds and satellite-based sea surface temperature (SST) observations on 0.25° grids. The satellite data produce a coupling coefficient of s[subscript U] = 0.42 m s⁻¹ °C⁻¹ for wind to mesoscale SST perturbations. The eight model configurations produce coupling coefficients varying from 0.31 to 0.56 m s⁻¹ °C⁻¹. Most closely matching QuikSCAT are a WRF simulation with the Grenier–Bretherton–McCaa (GBM) boundary layer mixing scheme (s[subscript U] = 0.40 m s⁻¹ °C⁻¹), and a COAMPS simulation with a form of Mellor–Yamada parameterization (s[subscript U] = 0.38 m s⁻¹ °C⁻¹). Model rankings based on coupling coefficients for wind stress, or for curl and divergence of vector winds and wind stress, are similar to that based on s[subscript U]. In all simulations, the atmospheric potential temperature response to local SST variations decreases gradually with height throughout the boundary layer (0–1.5 km). In contrast, the wind speed response to local SST perturbations decreases rapidly with height to near zero at 150–300 m. The simulated wind speed coupling coefficient is found to correlate well with the height-averaged turbulent eddy viscosity coefficient. The details of the vertical structure of the eddy viscosity depend on both the absolute magnitude of local SST perturbations, and the orientation of the surface wind to the SST gradient

Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII a in epileptic hippocampal neurons

Purpose: To investigate the mechanism of the anti-epileptic effect of Ganoderma lucidum polysaccharides (GLP), the changes of intracellular calcium and CaMK II a expression in a model of epileptic neurons were investigated. Method: Primary hippocampal neurons were divided into: 1) Control group, neurons were cultured with Neurobasal medium, for 3 hours; 2) Model group I: neurons were incubated with Mg2+ free medium for 3 hours; 3) Model group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with the normal medium for a further 3 hours; 4) GLP group I: neurons were incubated with Mg2+ free medium containing GLP (0.375 mg/ml) for 3 hours; 5) GLP group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with a normal culture medium containing GLP for a further 3 hours. The CaMK II a protein expression was assessed by Western-blot. Ca2+ turnover in neurons was assessed using Fluo-3/AM which was added into the replacement medium and Ca2+ turnover was observed under a laser scanning confocal microscope. Results: The CaMK II a expression in the model groups was less than in the control groups, however, in the GLP groups, it was higher than that observed in the model group. Ca2+ fluorescence intensity in GLP group I was significantly lower than that in model group I after 30 seconds, while in GLP group II, it was reduced significantly compared to model group II after 5 minutes. Conclusion: GLP may inhibit calcium overload and promote CaMK II a expression to protect epileptic neuron