890 research outputs found
The ancient inspirations for the German legal regulations on the incest
Since the German Ethic Council in September 2014 recommended revision of the incest law and questioned the justification for criminalising consensual sexual activity between siblings, the incest law became the subject of many public debates in Europe. This article is a contribution to show the evolution of the legal regulation of incest in Germany throughout the ages. A short overview of incest legislation in ancient cultures and in Roman law, shows that in nearly every past society, incest was considered taboo. Although, in theory, incest legislation in Roman law was very limited, it was in fact strictly forbidden. The present legal norms in Germany, which are based on Roman legal heritage and influenced by Christianity, also prohibit it. Incest is defined in § 173 of the German Criminal Code (Strafgesetzbuch) as an act of sexual intercourse between lineal ancestors and descendants and between siblings. The penalisation of incest is justified by protection of the family unit. Nevertheless, due to the radical changes in moral values of society, the current German legislation is facing a [email protected] w BiałymstokuAlbrecht H. J., Sieber U., Stellungnahme zu dem Fragenkatalog des Bundesverfassungsgerichts in dem Verfahren 2 BvR 392/07 zu § 173 Abs. 2 S. 2 StGB- Beischlaf zwischen Geschwistern, Frankfurt a Main 2007.Al-Zand A., Siebenhüner J., § 173 StGB – Eine kritische Betrachtung des strafrechtlichen Inzestverbots [w:] „Die Kritische Vierteljahresschrift für Gesetzgebung und Rechtswissenschaft“ 2006.Andrejew I., Polskie prawo karne, Warszawa 1989.Archibald E., Incest and the Medieval Imagination, Oxford 2001.Babusiaux U., Regelbildung durch exempla – Papinians Quaestiones zur ‘lex Iulia de adulteriis’ (Pal. 378), [w:] ‘Argumenta Papiniani’. Studien zur Geschichte und Dogmatik des Privatrechts, red. J. D. Harke, Berlin 2013.Barabas F., Sexualität und Recht, Frankfurt a. Main 2006.Bdeiwi S., Beischlaf zwischen Verwandten (§ 173 StGB).Reform und Gesetzgebung seit 1870, Berlin 2014.Bell H. J., Brother and Sister Mariage, «Revue Internationale des Droits de l’Antiquité» 1949, nr 2.Benincasa Z., ‘Deductio in domum mariti’ and the conclusion of a ‘iustum matrimonium’, „Miscellanea Historico-Iuridica” 2012, nr 11.Best G., Zur Aktualisierung des Inzestverbots: eine Erörterung anlässlich des Urteils des Bundesverfassungsgerichts, Münster 2010.Böckermann B., Cuny A., Das Bürgerliche Gesetzbuch mit besonderer Berücksichtigung der Rechtsprechung des Reichsgerichts und des Bundesgerichtshofes. Kommentar, Berlin 1999.Bradley M., Crime and Punishment on the Capitoline Hill, [w:] Rome, Pollution and Propriety. Dirt, Disease and Hygiene in the Eternal City from Antiquity to Modernity, red. M. Bradley, Cambridge 2012.Caldwell L. E., The Female Transition to Adulthood in the Early Roman Empire, Michigan 2004.Cerny J., Connsanguineos Marriages in Pharaonic Egipt, “Journal of Egypt Archeology” 1954, nr 40.Chouraqui A., Życie codzienne ludzi Biblii, tłum. L. Kossobudzki, Warszawa 1995.Cook S. A., The Laws of Moses and the Code of Hammurabi, London 1903.d’Almeras H., Małżeństwo u różnych narodów, tłum. M. Rodysowa, Warszawa 1904.De Vaux R., Ancient Israel. Its Life and Instructions, trans. J. McHugh, London 1961.De Vaux R., Les institutions de l’Ancien Testament, t. 1, Le nomadisme et ses survivances. Institutions familiales. Institutions civiles, Paris 1958.Driver G. R., Miles J. C. , The Babylonian Laws. Edited with Translation and Commentary, t. 1, Oxford 1952, t. 2, Oxford 1955.Dyjakowska M., Rodzina rzymska w poezji Horacego, [w:] Wokół problematyki małżeństwa w prawie rzymskim. Henrico Insadowski (1888-1946) in memoriam, red. A. Dębiński, M. Wójcik, Lublin 2007.Edmondson J. C., Granite Funerary Stelae from Augusta Emerita, Madrid 2006.Eming J., Jarzebowski C., Ulbrich C., Historische Inzestdiskurse, Königstein 2003.Ezazi G., Ethikräte in der Politik: Genese, Selbstverständnis und Arbeitsweise des Deutschen Ethikrates, Wiesbaden 2016.Fayer C., La familia romana. Aspetti giuridici ed antiquari, t. 2, Sponsalia, matrimonio, dote, t. 3, Concubinato, divorzio, adulterio, Roma 2005.Filar M., Przestępstwa seksualne w polskim prawie karnym, Toruń 1985.Flaceliere R., Życie codzienne w Grecji za czasów Peryklesa, tłum. Z. Bobowicz, J. Targalski, Warszawa 1985.Guarino A., Studi sull’ incestum, „Zeitschrift der Savigny-Stiftung für Rechtsgeschichte” 1943, nr 63.Héritier-Augé F., L’inceste dans les textes de la Grèce classique et post-classique, „Mètis. Anthropologie des mondes grecs anciens” 1994, nr 9-10.His R., Geschichte des deutschen Strafrechts bis zur Karolina, München – Berlin 1928.Insadowski H., Prawo rzymskie u Horacego, [w:] Wokół problematyki małżeństwa w prawie rzymskim. Henrico Insadowski (1888-1946) in memoriam, red. A. Dębiński, M. Wójcik, Lublin 2007.Insadowski H., Rzymskie prawo małżeńskie a chrześcijaństwo, [w:] H. Insadowski, Opera selecta, red. J. Misztal-Konecka, Lublin 2014.Jasnow R., Egipt. Middle Kingdom and Second Intermediate Period, [w:] A History of Ancient Near Eastern Law, red. R. Westbrook, t. 1, Leiden – Boston 2003.Kaczmarczyk A., Prawo spadkowe kobiet według Kodeksu z Gortyny, [w:] Pozycja prawna kobiet w dziejach, red. S. Rogowski, Kolonia 2010.Kodeks Hammurabiego, tłum. M. Stępień, edycja komputerowa, www.zrodla.hitoryczne.prv.pl (15.05.2016).Kołodko P., Chłosta jako dodatkowy środek karny zaostrzający dolegliwość kary właściwej, [w:] Salus rei publicae suprema lex: ochrona interesów państwa w prawie karnym Starożytnej Grecji i Rzymu, red. A. Dębiński, H. Kowalski, M. Kuryłowicz, Lublin 2007.Kołodko P., Prawne aspekty ograniczenia chłosty w prawie rzymskim, „Miscellanea Historico-Iuridica” 2006, nr 4.Koper S., Miłość, seks i polityka w starożytnych Grecji i Rzymie, Warszawa 1998.Krajewski R., Prawa i obowiązki seksualne małżonków: Studium prawne nad normą i patologią zachowań, Warszawa 2009.Krasowski K., Lesiński B., Sikorska-Dzięgielewska K., Walachowicz J., Powszechna historia państwa i prawa, Poznań 1993.Kruszyński P., Zasada domniemania niewinności w polskim procesie karnym, Warszawa 1983.Krzemińska A., Miłość w starożytnym Egipcie, Warszawa 2004.Kuleczka G., Prawo rzymskie epoki pryncypatu wobec dzieci pozamałżeńskich, Wrocław – Warszawa – Kraków 1969.Kulik M., Przedawnienie karalności i przedawnienie wykonania kary w polskim prawie karnym, Warszawa 2014.Kupiszewski H., Das Verlöbnis im altrömischen Recht, „Zeitschrift der Savigny-Stiftung für Rechtsgeschichte” 1960, nr 77.Kupiszewski H., Osservazioni sui rapporti patrimoniali fra i fidanzati nel diritto romano classico: „dos“ e „donatio“, „Rivista Internazionale di Diritto Romano e Antico” 1978, nr 29.Kupiszewski H., Stosunki majątkowe między narzeczonymi w prawie rzymskim klasycznym (dos i donatio), „Prawo Kanoniczne” 1977, nr 20.3-4.Kuryłowicz M., Prawo i obyczaje w starożytnym Rzymie, Lublin 1994.Kuryłowicz M., Wokół istoty małżeństwa rzymskiego, [w:] ‘Finis legis Christus’. Księga pamiątkowa dedykowana Księdzu Profesorowi Wojciechowi Góralskiemu z okazji siedemdziesiątej rocznicy urodzin, red. J. Wroceński, J. Krajczyński, t. 2, Warszawa 2009.Leipold K., Tsambikakis M., Zöller M. A., AnwaltKommentar StGB – Mitgliederausgabe AG Strafrecht, München 2014.Levick B., Women and Law, [w:] A Companion to Women in the Ancient World, red. S. L. James, S. Dillon, Chichester 2012.Lorsch-Wildfang R., Rome’s Vestal Virgins, London 2006.Luig K., Thomasius als Praktiker auf dem Gebiete des Privatrechts, [w:] Christian Thomasius (1655-1728). Neue Forschungen im Kontext der Frühaufk lärung, red. F. Vollhardt, Tubingen 1997.MacDowell D. M., Athenian Homicide Law. In the Age of the Orators, Manchester 1999.MacDowell D. M., The Law in Classical Athens, Ithaca 1986.Maciejewski T., Historia powszechna ustroju i prawa, Warszawa 2004.Masi Doria C., ‘Ancilla efficitur’... ‘In eo statu manebit’ le conseguenze del sc. claudianum per le donne di status libertino, [w:] ‘Mulier’. Algunas Historias e Instituciones de Derecho Romano, red. R. Rodríguez López, M. J. Bravo Bosch, Madrid 2013.McGinn T. A. J., Prostitution, Sexuality, and the Law in Ancient Rome, Oxford 1998.Meier Tetlow E., Women, Crime and Punishment in Ancient Law and Society, t. 1, The Ancient Near East, Londyn 2004.Mette-Dittmann A., Die Ehegesetze des Augustus. Eine Untersuchung im Rahmen der der Gesellschaftspolitik des Princeps, Stuttgart 1991.Mikołajczyk M., O niektórych przestępstwach obyczajowych w prawie miejskim w Polsce XVI-XVIIl wieku, „Problemy Prawa Karnego” 1994, nr 20.Misztal-Konecka J., Bigamia w prawie rzymskim, Lublin 2011.Misztal-Konecka J., ‘Incestum’ w prawie rzymskim, Lublin 2007.Misztal-Konecka J., Zakaz kazirodztwa w prawie rzymskim i prawodawstwie kościelnym (III-VI w.), „Zeszyty Prawnicze” 2011, nr 11.1.Modrzejewski J., Die Geschwisterehe in der hellenistischen Praxis und nach römischem Recht, „Zeitschrift der Savigny-Stiftung für Rechtsgeschichte” 1964, nr 81.Modrzejewski J., Małżeństwo między bratem a siostrą w grecko-rzymskim Egipcie, „Meander” 1955, nr 10.9-10.Modrzejewski J., Żydzi nad Nilem. Od Ramzesa II do Hadriana, Kraków 2000.Modzelewski K., Barbarzyńska Europa, Warszawa 2004.Moreau F., Incestus et prohibitae nuptiae: conception romaine de l’inceste et histoire des prohibitions matrimoniales pour cause de parenté dans la Rome antique, Paris 2002.Mossakowski W., ‘Iudicium domesticum w okresie republiki rzymskiej’ [w:] Rodzina w społeczeństwach antycznych i wczesnym chrześcijaństwie, Bydgoszcz 1995.Müller D. H., Die Gesetze Hammurabis und ihr Verhältnis zur Mosaischen Gesetzgebung sowie zu den XII Tafeln, Wien 1903.Myśliwiec K., Eros nad Nilem, Warszawa 1998.Niczyporuk P., Kompetencje pater familias względem dzieci w ustawach królewskich (leges regiae) w starożytnym Rzymie, [w:] Mężczyzna, Etyka, Ekonomia, Białystok 2011.Niczyporuk P., Żałoba i powtórne małżeństwo wdowy w prawie rzymskim, Białystok 2002.Palmen F., Der Inzest – Eine strafrechtlich-kriminologische Untersuchung, Köln 1968.Parker H. N., Why Were the Vestals Virgins?, [w:] Virginity Revisited. Configurations of the Unpossessed Body, red. B. Mac Lachlan, J. Fletcher, Toronto 2007.Polek K., Frankowie a ziemie nad środkowym Dunajem: przemiany polityczne i etniczne w okresie merowińskim i wczesnokarolińskim (do początku IX w.), Kraków 2007.Powirska M., Kazirodztwo w polskim prawie karnym, „Kortowski Przegląd Prawniczy” 2015, nr 2.Rein W., Das Criminalrecht der Römer von Romulus bis auf Justinian. Ein Hülfsbuch zur Erklaerung der Classiker, Leipzig 1844.Richardson J. S., Augustan Rome 44 BC to AD 14. The Restoration of the Republic and the Establishment of the Empire, Edinburg 2012.Romano A., ‘Matrimonium Iustum’, Napoli 1996.Rooke D. W., A Question of Sex?: Gender and Difference in the Hebrew Bible and Beyond, Sheffield 2007.Sanna M. V., Matrimonio e altre situazioni matrimoniali nel diritto romano classico. ‘Matrimonium iustum-matrimonium iniustum’, Napoli 2012.Schramm E., Ehe und Familie im Strafrecht: eine strafrechtsdogmatische Untersuchung, Tübingen 2011.Sójka-Zielińska K., Historia prawa, Warszawa 2015.Stolarek D., ‘Adultera‘ w świetle ‘lex Iulia de adulteriis coercendis‘, Lublin 2012.Szwejkowska M., Sitek B., Karnoprawny zakaz stosunków kazirodczych, „Studia Prawnoustrojowe” 2014, nr 23.Tamer D., Augustus Çağında Cinsel Suçlar ve Lex Iulia de adulteriis coercendis, Istanbul 2007.Taubenschlag R., Rzymskie prawo prywatne na tle praw antycznych, Warszawa 1955.Thierfelder H., Die Geschwisterchen im hellenistisch-römischen Ägypten, Münster 1960.Tittmann K., Handbuch der Strafrechtswissenschaft und der deutschen Strafgesetzkunde, Halle 1824.Versteeg R., Law in the Ancient World, Durham 2002.Volterra E., ‘Senatus consulta’, „Novissimo Digesto Italiano” 1969, nr 16.Warylewski J., Zakaz kazirodztwa w kodeksie karnym oraz w ujęciu porównawczym, „Przegląd Sądowy” 2001, nr 5.Watson A., The Law of Persons in the Later Roman Republic, Oxford 1967.Weyrauch V., Zulässigkeitsfragen und abstammungsrechtliche Folgeprobleme bei künstlicher Fortpflanzung im deutschen und US-amerikanischen Recht, Berlin 2003.Wilgaux J., Entre inceste et échange Réflexions sur le modèle matrimonial athénien, „L’homme” 2000, nr 154-155.Wittmann W., Die Blutschande – Eine rechtsgeschichtliche, rechtsvergleichende und kriminologische Untersuchung, unter Berücksichtigung der Nachkriegskriminalität in der Rheinpfalz, Mainz 1953.Wright D. P., Inventing God’s Law: How the Covenant Code of the Bible Used and Revised the Laws of Hammurabi, Oxford 2009.Zabłocka M., Przemiany prawa osobowego i rodzinnego w ustawodawstwie dynastii julijsko-klaudyjskiej, Warszawa 1987.11-3
Not only enthalpy: large entropy contribution to ion permeation barriers in single-file channels.
Health Service Providers\u27 Preferences in ICT Use for Health Service Delivery in Namibia
Health service providers (HSP) in both the private and public health service institutions in Namibia have adopted information and communication technologies (ICT) for health service delivery (HSD). The extent to which ICT are currently being used is, however, not yet fully known. Also unknown are the preferences of the HSPs in the use of different types of ICT applications and hardware. Lastly, the perception of HSPs on various issues on the use of ICT for health service delivery is also unknown. Without a clear understanding of these issues, it is difficult for government to formulate strategies and policies to promote the effective adoption of ICT in the healthcare sector. Patients are also likely to make a sub-optimal use of the available ICT to improve their access to health services in Namibia. A study was conducted to address these unknown points. The study highlights the importance of ICT use by HSPs and the imperative to align the ICT use expectations, needs and requirements of patients with those of HSPs in Namibia to ensure effective use. The results of the experiment are reported in this paper
Even With Rehydration, Preservation in Ethanol Influences the Mechanical Properties of Bone and How Bone Responds to Experimental Manipulation
Typically, bones are harvested at the time of animal euthanasia and stored until mechanical testing. However, storage methods are not standardized, and differential effects on mechanical properties are possible between methods. The goal of this study was to investigate the effects that two common preservation methods (freezing wrapped in saline-soaked gauze and refrigerating ethanol fixed samples) have on bone mechanical properties in the context of an in vitro ribosylation treatment designed to modify mechanical integrity. It was hypothesized that there would be an interactive effect between ribose treatment and preservation method. Tibiae from twenty five 11week old female C57BL/6 mice were separated into 2 preservation groups. Micro-CT scans of contralateral pairs assessed differences in geometry prior to storage. After 7weeks of storage, bones in each pair of tibiae were soaked in a solution containing either 0M or 0.6M ribose for 1week prior to 4 point bending tests. There were no differences in any cortical geometric parameters between contralateral tibiae. There was a significant main effect of ethanol fixation on displacement to yield (-16.3%), stiffness (+24.5%), strain to yield (-13.9%), and elastic modulus (+18.5%) relative to frozen specimens. There was a significant main effect of ribose treatment for yield force (+13.9%), ultimate force (+9.2%), work to yield (+22.2%), yield stress (+14.1%), and resilience (+21.9%) relative to control-soaked bones. Postyield displacement, total displacement, postyield work, total work, total strain, and toughness were analyzed separately within each preservation method due to significant interactions. For samples stored frozen, all six properties were lower in the ribose-soaked group (49%-68%) while no significant effects of ribose were observed in ethanol fixed bones. Storage in ethanol likely caused changes to the collagen matrix which prevented or masked the embrittling effects of ribosylation that were seen in samples stored frozen wrapped in saline-soaked gauze. These data illustrate the clear importance of maintaining hydration if the eventual goal is to use bones for mechanical assessments and further show that storage in ethanol can alter potential to detect effects of experimental manipulation (in this case ribosylation)
Application of the environmental relative moldiness index in Finland
The environmental relative moldiness index (ERMI) metric was previously developed to quantify mold contamination in U.S. homes. This study determined the applicability of the ERMI for quantifying mold and moisture damage in Finnish residences. Homes of the LUKAS2 birth cohort in Finland were visually inspected for moisture damage and mold, and vacuumed floor dust samples were collected. An ERMI analysis including 36 mold-specific quantitative PCR assays was performed on the dust samples (n = 144), and the ERMI metric was analyzed against inspection-based observations of moisture damage and mold. Our results show that the ERMI was significantly associated with certain observations of visible mold in Finnish homes but not with moisture damage. Several mold species occurred more frequently and at higher levels in Finnish than in U.S. homes. Modification of the ERMI toward Finnish conditions, using a subsample of LUKAS2 homes with and without moisture damage, resulted in a simplified metric based on 10 mold species. The Finnish ERMI (FERMI) performed substantially better in quantifying moisture and mold damage in Finnish homes, showing significant associations with various observations of visible mold, strongest when the damage was located in the child's main living area, as well as with mold odor and moisture damage. As shown in Finland, the ERMI as such is not equally well usable in different climates and geographic regions but may be remodeled to account for local outdoor and indoor fungal conditions as well as for moisture damage characteristics in a given country.Peer reviewe
Determinantes de la aceptación del "mobile learning" como elemento de formación del capital humano en las organizaciones
La revolución tecnológica ha modificado el modo de comunicarnos, realizar gran cantidad de transacciones, así como la forma de hacer negocios. Las organizaciones han tenido que adaptarse a los vertiginosos cambios y digitalizar gran parte de sus procesos productivos y operativa diaria (Messenger & Gschwind, 2016; Stone, Deadrick, Lukaszewski & Johnson, 2015). En su intento por obtener ventajas competitivas y un rendimiento empresarial mayor (Turulja & Bajgorić, 2016), las compañías invierten en activos intangibles mediante la generación de conocimiento (Hortovanyi & Ferincz, 2015). Es por ello que una de las áreas corporativas que se ha visto afectada por la emergente digitalización es la de la formación in-company. En este sentido, en las últimas décadas el e-learning se ha consolidado como una metodología formativa ampliamente utilizada por las compañías, respondiendo tanto a las necesidades empresariales como a las de los empleados. Si a ello le añadimos que actualmente prácticamente todos llevamos un dispositivo móvil que nos acompaña a donde quiera que vayamos (Little, 2013), que el mercado laboral cuenta con una fuerza de trabajo cada vez más digitalizada, y que con dichos dispositivos inalámbricos podemos realizar prácticamente las mismas operaciones que con un ordenador (Venkatesh, Thong & Xu, 2012), las organizaciones se encuentran ante la necesidad de ofrecer soluciones de aprendizaje en cualquier momento y lugar..
Directional deep brain stimulation for Parkinson's disease: results of an international crossover study with randomized, double-blind primary endpoint
Objective: Published reports on directional deep brain stimulation (DBS) have been limited to small, single-center investigations.
Therapeutic window (TW) is used to describe the range of stimulation amplitudes achieving symptom relief without side effects.
This crossover study performed a randomized double-blind assessment of TW for directional and omnidirectional DBS in a large
cohort of patients implanted with a DBS system in the subthalamic nucleus for Parkinson’s disease.
Materials and Methods: Participants received omnidirectional stimulation for the first three months after initial study pro gramming, followed by directional DBS for the following three months. The primary endpoint was a double-blind, randomized
evaluation of TW for directional vs omnidirectional stimulation at three months after initial study programming. Additional data
recorded at three- and six-month follow-ups included stimulation preference, therapeutic current strength, Unified Parkinson’s
Disease Rating Scale (UPDRS) part III motor score, and quality of life.
Results: The study enrolled 234 subjects (62 ± 8 years, 33% female). TW was wider using directional stimulation in 183 of 202
subjects (90.6%). The mean increase in TW with directional stimulation was 41% (2.98 ± 1.38 mA, compared to 2.11 ± 1.33 mA for
omnidirectional). UPDRS part III motor score on medication improved 42.4% at three months (after three months of omnidi rectional stimulation) and 43.3% at six months (after three months of directional stimulation) with stimulation on, compared to
stimulation off. After six months, 52.8% of subjects blinded to stimulation type (102/193) preferred the period with directional
stimulation, and 25.9% (50/193) preferred the omnidirectional period. The directional period was preferred by 58.5% of clinicians
(113/193) vs 21.2% (41/193) who preferred the omnidirectional period.
Conclusion: Directional stimulation yielded a wider TW compared to omnidirectional stimulation and was preferred by blinded
subjects and clinicians
Current concepts in stereotactic radiosurgery - a neurosurgical and radiooncological point of view
Stereotactic radiosurgery is related to the history of "radiotherapy" and "stereotactic neurosurgery". The concepts for neurosurgeons and radiooncologists have been changed during the last decade and have also transformed neurosurgery. The gamma knife and the stereotactically modified linear accelerator (LINAC) are radiosurgical equipments to treat predetermined intracranial targets through the intact skull without damaging the surrounding normal brain tissue. These technical developments allow a more precise intracranial lesion control and offer even more conformal dose plans for irregularly shaped lesions. Histological determination by stereotactic biopsy remains the basis for any otherwise undefined intracranial lesion. As a minimal approach, it allows functional preservation, low risk and high sensitivity. Long-term results have been published for various indications. The impact of radiosurgery is presented for the management of gliomas, metastases, brain stem lesions, benign tumours and vascular malformations and selected functional disorders such as trigeminal neuralgia. In AVM's it can be performed as part of a multimodality strategy including resection or endovascular embolisation. Finally, the technological advances in radiation oncology as well as stereotactic neurosurgery have led to significant improvements in radiosurgical treatment opportunities. Novel indications are currently under investigation. The combination of both, the neurosurgical and the radiooncological expertise, will help to minimize the risk for the patient while achieving a greater treatment success
Mycotoxin Adducts on Human Serum Albumin: Biomarkers of Exposure to Stachybotrys chartarum
OBJECTIVE: Despite the growing body of evidence showing adverse health effects from inhalation exposure to the trichothecene-producing mold Stachybotrys chartarum, controversy remains. Currently, there are no reliable assays suitable for clinical diagnosis of exposure. We hypothesized that satratoxin G (SG)–albumin adducts may serve as biomarkers of exposure to this fungus. DESIGN: We studied the formation of adducts of SG with serum albumin in vitro using Western blots and mass spectrometry (MS) and searched for similar adducts formed in vivo using human and animal serum. RESULTS: Samples of purified human serum albumin that had been incubated with increasing concentrations of SG showed concentration-dependent albumin bands in Western blots developed with anti-SG antibodies. MS analysis found that as many as 10 toxin molecules can be bound in vitro to one albumin molecule. The sequencing of albumin-adduct tryptic peptides and the analysis of pronase/aminopeptidase digests demonstrated that lysyl, cysteinyl, and histidyl residues are involved in the formation of these adducts. Serum samples from three patients with documented exposure to S. chartarum similarly revealed lysine–, cysteine–, and histidine–SG adducts after exhaustive digestion, affinity column enrichment, and MS analysis. These adducts were also found in the sera from rats exposed to the spores of S. chartarum in contrast to control human subjects and control animals. CONCLUSIONS: These data document the occurrence of SG–albumin adducts in both in vitro experiments and in vivo human and animal exposures to S. chartarum. RELEVANCE TO CLINICAL PRACTICE: SG–amino acid adducts may serve as reliable dosimeter biomarkers for detection of exposure to S. chartarum
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Hydrogel Tracer Beads: The Development, Modification, and Testing of an Innovative Tracer for Better Understanding LNAPL Transport in Karst Aquifers
The goal of this specific research task is to develop proxy tracers that mimic contaminant movement to better understand and predict contaminant fate and transport in karst aquifers. Hydrogel tracer beads are transported as a separate phase than water and can used as a proxy tracer to mimic the transport of non-aqueous phase liquids (NAPL). They can be constructed with different densities, sizes & chemical attributes. This poster describes the creation and optimization of the beads and the field testing of buoyant beads, including sampling, tracer analysis, and quantitative analysis. The buoyant beads are transported ahead of the dissolved solutes, suggesting that light NAPL (LNAPL) transport in karst may occur faster than predicted from traditional tracing techniques. The hydrogel beads were successful in illustrating this enhanced transport
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