160 research outputs found
Crystalline structure and orientation of gold clusters grown in preformed nanometer-sized pits
Abstract Gold clusters were produced by condensing evaporated gold in nanometer-sized preformed pits on the surface of highly Ćœ . oriented pyrolytic graphite HOPG . The height of the clusters was 6.7 " 0.7 nm as measured with scanning tunneling microscopy in ultrahigh vacuum, the lateral width was 10.1 " 1.9 nm as determined with transmission electron microscopy Ćœ . TEM . Using TEM for electron diffraction, we obtained information on the crystalline structure of the clusters. The Ćœ . intensity of the observed diffraction rings shows the preferential orientation of the clusters with the 111 plane of the gold Ćœ . lattice parallel to the 0001 surface of HOPG. This was compared to the diffraction pattern of gold clusters produced in the gas phase by inert-gas evaporation and deposited on a flat HOPG surface at room temperature as complete units which showed no preferential orientation. The directional alignment in the surface plane as it is described in the literature for larger gold crystallites grown on a flat HOPG surface is not observed for the nanometer-sized clusters grown in pits
Insights into the chemical composition of Equisetum hyemale by high resolution Raman imaging
Equisetaceae has been of research interest for decades, as it is one of the oldest living plant families, and also due to its high accumulation of silica up to 25% dry wt. Aspects of silica deposition, its association with other biomolecules, as well as the chemical composition of the outer strengthening tissue still remain unclear. These questions were addressed by using high resolution (<1Â ÎŒm) Confocal Raman microscopy. Two-dimensional spectral maps were acquired on cross sections of Equisetum hyemale and Raman images calculated by integrating over the intensity of characteristic spectral regions. This enabled direct visualization of differences in chemical composition and extraction of average spectra from defined regions for detailed analyses, including principal component analysis (PCA) and basis analysis (partial least square fit based on model spectra). Accumulation of silica was imaged in the knobs and in a thin layer below the cuticula. In the spectrum extracted from the knob region as main contributions, a broad band below 500Â cmâ1 attributed to amorphous silica, and a band at 976Â cmâ1 assigned to silanol groups, were found. From this, we concluded that these protrusions were almost pure amorphous, hydrated silica. No silanol group vibration was detected in the silicified epidermal layer below and association with pectin and hemicelluloses indicated. Pectin and hemicelluloses (glucomannan) were found in high levels in the epidermal layer and in a clearly distinguished outer part of the hypodermal sterome fibers. The inner part of the two-layered cells revealed as almost pure cellulose, oriented parallel along the fiber
Palynomorphs of the Normapolles group and related plant mesofossils from the IharkĂșt vertebrate site, Bakony Mountains (Hungary)
Abstract
Palynological and paleobotanical investigation of bonebeds and other strata of the CsehbĂĄnya Formation from the vertebrate locality at IharkĂșt (Bakony Mts, Hungary) reveals well-preserved Santonian palynological assemblages dominated by the Normapolles group, with a minor component consisting of other angiosperm pollen, some gymnosperm pollen, and spores. Eleven species of Normapolles-type pollen grains belonging to seven genera and fruit remains of a new taxon, Sphaeracostata barbackae gen. et sp. nov., are described. The new species is very abundant in the material, represented by ca. 1000 specimens. The genus Caryanthus Friis and an unnamed form previously reported from HaĆŁeg by Lindfors et al. (2010) are also present.
Plants producing Normapolles-type pollen grains diversified during the Late Cretaceous, with a bloom in the Santonian. The palynostratigraphy of the Upper Cretaceous terrestrial sediments in the studied region is based on Normapolles-related species. The studied assemblage is assigned to the Oculopollis zaklinskaiae-Tetracolporopollenites (Brecolpites) globosus Zone (or Zone C) indicating a late Santonian age. Comparison of the IharkĂșt palynoflora with other known Upper Cretaceous palynofloras of Central Europe shows diachronous occurrence of Normapolles taxa at different geographic localities and warrants further investigation.
The ecological requirements of the amphibian fauna reflect azonal conditions controlled by the availability of water, which is in agreement with the inferred ecological conditions based on the paleobotanical investigations. The fauna is of entirely non-marine character, further supported by isotope studies, in line with our data showing that the palynological samples contain no marine forms
Contribution to Early Holocene vegetation and climate history of Eastern Orinoco Llanos, Venezuela, from a paleoecological record of a Mauritia L.f. swamp
ăăŁăȘăăłăźć°ć ăăłă°ăŠă€ă«ăčææçăźéçćă«ăHLA-A*33:01ăąăŹă«ăŻéČćŸĄçă«ćă
Dengue virus infection is a leading cause of morbidity among children in the Philippines in recent years. In order to investigate the association of HLA Class I and II alleles and dengue disease severity in a cohort of Filipino children, we performed a case control study in 2 hospitals in Metro Manila from June 2008 to December 2009. A total of 250 laboratory confirmed dengue patients and 300 healthy individuals aged 5 to 15 years old were typed for HLA-A, B and DRB1 alleles. The frequency of HLA-A*33:01 was significantly decreased in severe dengue (DHF/ DSS; Pc = 0.0016)) and DSS (Pc = 0.0032) compared to the background population. These findings support a previous study that this allele may confer protection against the severe form of dengue and provide the first evidence of HLA association with dengue in the Philippines. Future studies should be directed in investigating the possible mechanisms of protection.é·ćŽć€§ćŠćŠäœè«æ ćŠäœèšçȘć·:ć(ć»æŻèŹ)äč珏39ć· ćŠäœæäžćčŽææ„:ćčłæ27ćčŽ6æ3æ„Author: Edelwisa Segubre Mercado, Fe Esperanza Espino, Ma. Lucila M. Perez, Josie M. Bilar, Jemimah Dawn P. Bajaro, Nguyen Tien Huy, Benilda Q Baello, Mihoko Kikuchi, Kenji HirayamaCitation: PLOS ONE, 10(2), e0115619; 2015Nagasaki University (é·ćŽć€§ćŠ)è«æć
High-resolution metabolomics to discover potential parasite-specific biomarkers in a Plasmodium falciparum erythrocytic stage culture system
First evidence for a massive extinction event affecting bees close to the K-T boundary
Bees and eudicot plants both arose in the mid-late Cretaceous, and their co-evolutionary relationships have often been assumed as an important element in the rise of flowering plants. Given the near-complete dependence of bees on eudicots we would expect that major extinction events affecting the latter would have also impacted bees. However, given the very patchy distribution of bees in the fossil record, identifying any such extinctions using fossils is very problematic. Here we use molecular phylogenetic analyses to show that one bee group, the Xylocopinae, originated in the mid-Cretaceous, coinciding with the early radiation of the eudicots. Lineage through time analyses for this bee subfamily show very early diversification, followed by a long period of seemingly no radiation and then followed by rapid diversification in each of the four constituent tribes. These patterns are consistent with both a long-fuse model of radiation and a massive extinction event close to the K-T boundary. We argue that massive extinction is much more plausible than a long fuse, given the historical biogeography of these bees and the diversity of ecological niches that they occupy. Our results suggest that events near the K-T boundary would have disrupted many plant-bee relationships, with major consequences for the subsequent evolution of eudicots and their pollinators.Sandra M. Rehan, Remko Leys, Michael P. Schwar
Phylogenetic assessment of Chaetomium indicum and allied species, with the introduction of three new species and epitypification of C. funicola and C. indicum
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