Functional and Predictive Structural Characterization of WRINKLED2, A Unique Oil Biosynthesis Regulator in Avocado

WRINKLED1 (WRI1), a member of the APETALA2 (AP2) class of transcription factors regulates fatty acid biosynthesis and triacylglycerol (TAG) accumulation in plants. Among the four known Arabidopsis WRI1 paralogs, only WRI2 was unable to complement and restore fatty acid content in wri1-1 mutant seeds. Avocado (Persea americana) mesocarp, which accumulates 60-70% dry weight oil content, showed high expression levels for orthologs of WRI2, along with WRI1 and WRI3, during fruit development. While the role of WRI1 as a master regulator of oil biosynthesis is well-established, the function of WRI1 paralogs is poorly understood. Comprehensive and comparative in silico analyses of WRI1 paralogs from avocado (a basal angiosperm) with higher angiosperms Arabidopsis (dicot), maize (monocot) revealed distinct features. Predictive structural analyses of the WRI orthologs from these three species revealed the presence of AP2 domains and other highly conserved features, such as intrinsically disordered regions associated with predicted PEST motifs and phosphorylation sites. Additionally, avocado WRI proteins also contained distinct features that were absent in the nonfunctional Arabidopsis ortholog AtWRI2. Through transient expression assays, we demonstrated that both avocado WRI1 and WRI2 are functional and drive TAG accumulation in Nicotiana benthamiana leaves. We predict that the unique features and activities of ancestral PaWRI2 were likely lost in orthologous genes such as AtWRI2 during evolution and speciation, leading to at least partial loss of function in some higher eudicots. This study provides us with new targets to enhance oil biosynthesis in plants

Three fermions in a box at the unitary limit: universality in a lattice model

We consider three fermions with two spin components interacting on a lattice model with an infinite scattering length. Low lying eigenenergies in a cubic box with periodic boundary conditions, and for a zero total momentum, are calculated numerically for decreasing values of the lattice period. The results are compared to the predictions of the zero range Bethe-Peierls model in continuous space, where the interaction is replaced by contact conditions. The numerical computation, combined with analytical arguments, shows the absence of negative energy solution, and a rapid convergence of the lattice model towards the Bethe-Peierls model for a vanishing lattice period. This establishes for this system the universality of the zero interaction range limit.Comment: 6 page

Tagging of Explosives for Detection

This paper gives the results of a study on estimation of shelf life of2,3-dimethyI2,3-dinitrobutane (DMNB)-tagged RDX and PETN expiosives by monitoring DMNB depletion by high performanceliquid chromatography and simultaneously recording the detectability of the tagged explosive composition using explosive vapoUf detector Model-97 HS. DMNB was incorporated in the explosive using methanol as solvent for DMNB and the explosive compositions were stored at 35,55 and 75 °C over a long period. Methods developed for preparing the homogeneously tagged composition with DMNB at 0.5 per cent level and for the analysis ofDMNB for ensuring homogeneity of DMNB in the composition are described. The results show no change in compatibility and sensitivity on the incorporation of DMNB in the explosive. Estimation of shelf life of DMNB in the explosive was done for a period of storage of 202-304 days at different temperatures

MENINGKATKAN LITERASI MEMBACA MELALUI MAJALAH DIGITAL DI TAMAN BACA PEKAN KARYA (PEKA) PAMULANG

Menghadapi dunia yang serba modern ini tentunya diperlukan Sumber Daya Manusia (SDM) yang berkualitas. Salah satu langkah yang harus segera dilakukan adalah dalam hal literasi membaca. Literasi membaca memegang peranan penting dalam meningkatkan kualitas pendidikan. Indonesia masih berada di peringkat rendah dalam hal membaca. Seperti yang dapat kita lihat saat ini, generasi muda enggan menyempatkan waktunya untuk sekadar membaca beberapa halaman buku. Dalam hal ini, konteks membaca yang dimaksud adalah membaca untuk pemahaman suatu teks. Untuk mencapai hal tersebut tentunya dibutuhkan tenaga pendidik yang juga mendukung dalam hal memahami teks. Dukungan tersebut dapat diwujudkan dengan adanya metode khusus yang dapat digunakan untuk membantu anak-anak dalam memahami suatu teks. Berdasarkan hal tersebut, kelompok PKM ini ingin ikut berkontribusi dalam mewujudkan peningkatan pemahaman membaca pada anak usia dini, khususnya di Taman Baca Pekan Karya (PEKA) di Pamulang, Tangerang Selatan. Jadi, tujuan pengabdian masyarakat ini adalah untuk meningkatkan pemahaman teks bacaan kepada masyarakat khususnya anak-anak sekitar Taman Baca Pekan Karya (PEKA) di Tangerang Selatan melalui majalah digital. Subjek penelitian dalam PKM ini adalah masyarakat sekitar Pamulang, Tangerang Selatan. Pengabdian dilakukan secara tatap muka. Target luaran dari kegiatan pengabdian masyarakat ini adalah publikasi di jurnal nasional terindeks SINTA. Kata Kunci: Literasi, Membaca, Majalah Digita

Autonomy found: Estimating the local benefit from tourism in SIDS – the Case of Cape Verde

Tourism is an unusually important economic sector for the Small Island Development States (SIDS). Islands are the second most visited destinations after historic cities. The SIDS tourism is usually dependent on foreign investment and in the form of All-Inclusive (AI) system. Few studies have evaluated the economic impact of tourism on the economic development and/or poverty reduction of a SID using macro level data. And little research has used micro/firm level data to investigate the impact of tourism on the local economy of SIDS. This paper aims to evaluate the local benefits made from the development of tourism in SIDS, in particular the contribution of AI accommodation using the case of Cape Verde. It makes two main contributions: it makes the first attempt to use microeconomic data at the company level to examine the contribution of tourism in SIDS; it uses empirical data to examine the impacts of AI accommodation on the local economy. A semi-structured hotel questionnaire was applied to 13 accommodation managers and hotel owners, from small guest-houses to large 500 room All-Inclusive resorts in Sal, Boa Vista, Fogo, and Santo Antão. The results indicate that for destinations at an early stage in their development, large-scale AIs may be the most effective way to achieve the growth of tourism to the point that a critical mass is achieved

Enhancing a de novo enzyme activity by computationally-focused ultra-low-throughput screening

Directed evolution has revolutionized protein engineering. Still, enzyme optimization by random library screening remains sluggish, in large part due to futile probing of mutations that are catalytically neutral and/or impair stability and folding. FuncLib is a novel approach which uses phylogenetic analysis and Rosetta design to rank enzyme variants with multiple mutations, on the basis of predicted stability. Here, we use it to target the active site region of a minimalist-designed, de novo Kemp eliminase. The similarity between the Michaelis complex and transition state for the enzymatic reaction makes this system particularly challenging to optimize. Yet, experimental screening of a small number of active-site variants at the top of the predicted stability ranking leads to catalytic efficiencies and turnover numbers ( 2 104 M 1 s 1 and 102 s 1) for this anthropogenic reaction that compare favorably to those of modern natural enzymes. This result illustrates the promise of FuncLib as a powerful tool with which to speed up directed evolution, even on scaffolds that were not originally evolved for those functions, by guiding screening to regions of the sequence space that encode stable and catalytically diverse enzymes. Empirical valence bond calculations reproduce the experimental activation energies for the optimized eliminases to within 2 kcal mol 1 and indicate that the enhanced activity is linked to better geometric preorganization of the active site. This raises the possibility of further enhancing the stabilityguidance of FuncLib by computational predictions of catalytic activity, as a generalized approach for computational enzyme designKnut and Alice Wallenberg Foundation (Wallenberg Academy Fellowship) 2018.0140Human Frontier Science Program RGP0041/2017FEDER Funds/Spanish Ministry of Science, Innovation and Universities BIO2015-66426-R RTI2018-097142-B-100FEDER/Junta de Andalucia - Consejeria de Economia y Conocimiento E.FQM.113.UGR18Swedish National Infrastructure for computing (SNAC) 2018/2-3 2019/2-

Diminishing benefits of urban living for children and adolescents’ growth and development

Optimal growth and development in childhood and adolescence is crucial for lifelong health and well-being1,2,3,4,5,6. Here we used data from 2,325 population-based studies, with measurements of height and weight from 71 million participants, to report the height and body-mass index (BMI) of children and adolescents aged 5–19 years on the basis of rural and urban place of residence in 200 countries and territories from 1990 to 2020. In 1990, children and adolescents residing in cities were taller than their rural counterparts in all but a few high-income countries. By 2020, the urban height advantage became smaller in most countries, and in many high-income western countries it reversed into a small urban-based disadvantage. The exception was for boys in most countries in sub-Saharan Africa and in some countries in Oceania, south Asia and the region of central Asia, Middle East and north Africa. In these countries, successive cohorts of boys from rural places either did not gain height or possibly became shorter, and hence fell further behind their urban peers. The difference between the age-standardized mean BMI of children in urban and rural areas wa

Liberating Efimov physics from three dimensions

When two particles attract via a resonant short-range interaction, three particles always form an infinite tower of bound states characterized by a discrete scaling symmetry. It has been considered that this Efimov effect exists only in three dimensions. Here we review how the Efimov physics can be liberated from three dimensions by considering two-body and three-body interactions in mixed dimensions and four-body interaction in one dimension. In such new systems, intriguing phenomena appear, such as confinement-induced Efimov effect, Bose-Fermi crossover in Efimov spectrum, and formation of interlayer Efimov trimers. Some of them are observable in ultracold atom experiments and we believe that this study significantly broadens our horizons of universal Efimov physics.Comment: 17 pages, 5 figures, contribution to a special issue of Few-Body Systems devoted to Efimov Physic