Búsqueda de variabilidad genotípica en la capacidad de colonización de hongos micorricicos arbusculares-soja y su efecto en el crecimiento

La simbiosis de hongos micorricicos arbusculares (HMA)-planta es una estrategia de creciente interés desde que se la ha relacionado con la mitigación del estrés abiótico y biótico y con el aumento en el rendimiento de los cultivos. Sin embargo existen antecedentes que muestran que la aplicación de fertilizantes ha influenciado hacia la selección de genotipos menos micorrizables. El objetivo de este trabajo fue aportar conocimiento acerca de la posible variabilidad en la capacidad de colonización de HMA, en raíces de genotipos de soja y cuál es su efecto en el crecimiento de la planta.Instituto de Botánica "Dr. Carlos Spegazzini

Desarrollo de estrategias experimentales para la evaluación de la simbiosis hongos micorrícicos arbusculares nativos-Prosopis alba en condiciones controladas

Debido a la importancia creciente de la simbiosis HMA –planta en la mitigación del estrés biótico/abiótico en las plantas, así como a la necesidad de mejorar la implantación del algarrobo en zonas semiáridas de Argentina, se desarrolló un sistema experimental modelo, para demostrar el efecto de dos inóculos de HMA nativos, sobre el crecimiento de Prosopis alba Griseb. Para ello se aislaron dos inóculos de suelos de algarrobales de condiciones pluviométricas contrastantes: Padre Lozano (PL), 650mm de pp) de la provincia de Salta y Colonia Benítez (CB) 1300 mm de pp de la provincia del Chaco, ambos pertenecientes al Parque Chaqueño Argentino.Instituto de Botánica "Dr. Carlos Spegazzini"Comisión de Investigaciones Científicas de la provincia de Buenos Aire

Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a singletube ‘hanging droplet’ PCR assay with modified pan-PV CODEHOP primers

A modified pan-PV consensus-degenerate hybrid oligonucleotide primer (CODEHOP) PCR was developed for generic and sensitive detection of a broad-spectrum of human papillomaviruses (HPVs) infecting the cutaneous epithelium. To test the analytical sensitivity of the assay we examined 149 eyebrow hair follicle specimens from immunocompetent male patients. HPV DNA was detected in 60% (89/149) of analysed eyebrow samples with a total of 48 different HPV sequences, representing 21 previously described HPVs and 27 putative novel HPV types. Evidence for ten novel HPV subtypes and seven viral variants, clustering to three out of five genera containing cutaneous HPVs, was also obtained. Thus, we have shown that the modified pan-PV CODEHOP PCR assay is able to identify multiple HPV types, even from different genera, in the same clinical sample. Overall, these results demonstrate that the pan-PV CODEHOP PCR is an excellent tool for screening and identification of novel cutaneous HPVs, even in samples with low viral loads.Fil: Chouhy, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Kocjan, Boštjan J.. University of Ljubljana; EsloveniaFil: Staheli, Jeannette P.. Seattle Children’s Research Institute; Estados UnidosFil: Bolatti, Elisa Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Hošnjak, Lea. University of Ljubljana; EsloveniaFil: Sagadin, Martin. University of Ljubljana; EsloveniaFil: Giri, Adriana Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Rose, Timothy M.. Seattle Children’s Research Institute; Estados UnidosFil: Poljak, Mario. University of Ljubljana; Esloveni

Interactions between mineral fertilization and arbuscular mycorrhizal fungi improve nursery growth and drought tolerance of Prosopis alba seedlings

Prosopis alba Griseb. (algarrobo blanco) is an important tree legume in semiarid regions of South America. Under field conditions, their seedlings are subjected to a wide range of environmental conditions that might produce stress, reducing their survival and/or growth. The aim of this work was to evaluate the combined effect of two native arbuscular mycorrhizal fungi (AMF) inocula and fertilization on growth variables of P. alba seedlings under nursery conditions and after drought stress. P. alba seedlings received different levels of basic fertilization (0%, 25% and 100%) and were inoculated with native AMF isolated from different rainfall regions of Parque Chaqueño Argentino: Colonia Benítez (CB, 1300 mm rainfall) and Padre Lozano (PL, 650 mm rainfall) or a mixture of both inocula (MIX), while the control group remained uninoculated. The combined application of native AMF and fertilization did not affect mycorrhizal colonization but allowed the formation of the different AMF structures. Moreover, when comparing the PL inoculum from a low rainfall region with other treatments, it significantly increased growth and development under nursery conditions and drought stress tolerance in the greenhouse. Therefore, AMF benefits for P. alba were related to inoculum source, being improved by the application of low fertilizer rates. This work supports the development of sustainable P. alba seedlings production and their field establishment under symbiotic conditions with native AMF

Climatic characterization of the wheat streak mosaic virus (WSMV) pathology system and its vector Aceria tosichella Keifer using a Geographical Information System (GIS)

Abstracts del XXIV ANNUAL SCIENTIFIC MEETING, celebrado del 8-10 de noviembre 2007, en Tafí del Valle, Tucumán, Argentina.In Argentina, the Wheat streak mosaic virus (WSMV) disease has been present since 2003, when it was detected for the first time in the districts of Marcos Juárez and Jesús María, province of Córdoba. Since then, the disease has been rapidly expanding. This study was undertaken for the purpose of characterizing through a geographical information system (GIS) the areas where the disease was detected up to the present. Data were used for 28 sites where virosis was detected during the 2003-2006 period. FloraMap 1.02 software was used to characterize the sites. Maximum and minimum altitudes recorded were 1280 and 30 m above sea level respectively. Maximum and minimum average ambient temperatures (AAT) were 27.9ºC and 6.1ºC respectively. Maximum and minimum average environmental pressure (AEP) was 239 mm and 2 mm respectively. All sites had a dry season lasting from 6 to 11 months. These results will contribute to an epidemiological study by providing data on climatic features.Peer reviewe

Aceria tosichella keifer (Acari: Eriophyidae) from wheat streak mosaic virus-infected wheat plants in Argentina.

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