Visual tracking of small animals in cluttered natural environments using a freely moving camera

Image-based tracking of animals in their natural habitats can provide rich behavioural data, but is very challenging due to complex and dynamic background and target appearances. We present an effective method to recover the positions of terrestrial animals in cluttered environments from video sequences filmed using a freely moving monocular camera. The method uses residual motion cues to detect the targets and is thus robust to different lighting conditions and requires no a-priori appearance model of the animal or environment. The detection is globally optimised based on an inference problem formulation using factor graphs. This handles ambiguities such as occlusions and intersections and provides automatic initialisation. Furthermore, this formulation allows a seamless integration of occasional user input for the most difficult situations, so that the effect of a few manual position estimates are smoothly distributed over long sequences. Testing our system against a benchmark dataset featuring small targets in natural scenes, we obtain 96% accuracy for fully automated tracking. We also demonstrate reliable tracking in a new data set that includes different targets (insects, vertebrates or artificial objects) in a variety of environments (desert, jungle, meadows, urban) using different imaging devices (day / night vision cameras, smart phones) and modalities (stationary, hand-held, drone operated)

Towards Sustainability Assessment of the Built Environment: A Classification of the Existing Challenges

The application of sustainability assessment in a decision context is associated with various challenges that explain why the transition to action-oriented knowledge still needs to be fulfilled. Therefore, this paper aims to explore the associated challenges in sustainability assessment in the decision context of the built environment. Several publications are reviewed to provide a systemic understanding of the associated complexities. The challenges in sustainability assessment in the built environment are categorized at different levels, from understanding to measurement and implementation. The challenges are further categorized into definition, context, interpretation, data, measurement methods, uncertainties, indicators and indices, results, coordination, conflicts, and action-oriented knowledge. Moreover, according to the nature of each challenge, they are classified into epistemological, methodological, and procedural challenges. The novelty of this review is that it reviews and reports almost all fragmentedly reported challenges in sustainability assessment of the built environment in the literature within a holistic framework that provides a clear understanding of the state of the art and second discusses them within an integrated framework (the Sustainability Assessment Network) including the position of active-role players to resolve them, including strategists, scientist, and stakeholders

Optimal Balance between Heating, Cooling and Environmental Impacts: A Method for Appropriate Assessment of Building Envelope’s U-Value

In Europe, the recent application of regulations oriented to zero-energy buildings and climate neutrality in 2050 has led to a reduction in energy consumption for heating and cooling in the construction sector. The thermal insulation of the building envelope plays a key role in this process and the requirements about the maximum allowable thermal transmittance are defined by country-specific guidelines. Typically, high insulation values provide low energy consumption for heating; however, they may also entail a risk of overheating in summer period and thus negatively affect the overall performance of the building. In addition, the embodied energy and related emissions caused by the manufacturing and transportation processes of thermal insulation cannot be further neglected in the evaluation of the best optimal solution. Therefore, this paper aims to evaluate the influence in terms of embodied and operational energy of various walls’ thermal insulation thicknesses on residential buildings in Europe. To this end, the EnergyPlus engine was used for the energy simulation within the Ladybug and Honeybee tools, by parametrically conducting multiple iterations; 53 variations of external wall U-value, considering high- and low-thermal-mass scenarios, were simulated for 100 representative cities of the European context, using a typical multifamily building as a reference. The results demonstrate that massive walls generally perform better than lightweight structures and the best solution in terms of energy varies according to each climate. Accordingly, the wall’s thermal transmittance for the samples of Oslo, Bordeaux, Rome and Almeria representative of the Continental, oceanic temperate, Mediterranean, and hot, semi-arid climates were, respectively: 0.12, 0.26, 0.42, and 0.64 W/m2K. The optimal solutions are graphically reported on the map of Europe according to specific climatic features, providing a guidance for new constructions and building retrofit

The composition and functional protein subsystems of the human nasal microbiome in granulomatosis with polyangiitis: a pilot study

Abstract: Background: Ear, nose and throat involvement in granulomatosis with polyangiitis (GPA) is frequently the initial disease manifestation. Previous investigations have observed a higher prevalence of Staphylococcus aureus in patients with GPA, and chronic nasal carriage has been linked with an increased risk of disease relapse. In this cross-sectional study, we investigated changes in the nasal microbiota including a detailed analysis of Staphylococcus spp. by shotgun metagenomics in patients with active and inactive granulomatosis with polyangiitis (GPA). Shotgun metagenomic sequence data were also used to identify protein-encoding genes within the SEED database, and the abundance of proteins then correlated with the presence of bacterial species on an annotated heatmap. Results: The presence of S. aureus in the nose as assessed by culture was more frequently detected in patients with active GPA (66.7%) compared with inactive GPA (34.1%). Beta diversity analysis of nasal microbiota by bacterial 16S rRNA profiling revealed a different composition between GPA patients and healthy controls (P = 0.039). Beta diversity analysis of shotgun metagenomic sequence data for Staphylococcus spp. revealed a different composition between active GPA patients and healthy controls and disease controls (P = 0.0007 and P = 0.0023, respectively), and between healthy controls and inactive GPA patients and household controls (P = 0.0168 and P = 0.0168, respectively). Patients with active GPA had a higher abundance of S. aureus, mirroring the culture data, while healthy controls had a higher abundance of S. epidermidis. Staphylococcus pseudintermedius, generally assumed to be a pathogen of cats and dogs, showed an abundance of 13% among the Staphylococcus spp. in our cohort. During long-term follow-up of patients with inactive GPA at baseline, a higher S. aureus abundance was not associated with an increased relapse risk. Functional analyses identified ten SEED protein subsystems that differed between the groups. Most significant associations were related to chorismate synthesis and involved in the vitamin B12 pathway. Conclusion: Our data revealed a distinct dysbiosis of the nasal microbiota in GPA patients compared with disease and healthy controls. Metagenomic sequencing demonstrated that this dysbiosis in active GPA patients is manifested by increased abundance of S. aureus and a depletion of S. epidermidis, further demonstrating the antagonist relationships between these species. SEED functional protein subsystem analysis identified an association between the unique bacterial nasal microbiota clusters seen mainly in GPA patients and an elevated abundance of genes associated with chorismate synthesis and vitamin B12 pathways. Further studies are required to further elucidate the relationship between the biosynthesis genes and the associated bacterial species

POZ-, AT-hook-, and Zinc Finger-containing Protein (PATZ) Interacts with Human Oncogene B Cell Lymphoma 6 (BCL6) and Is Required for Its Negative Autoregulation.

The PATZ1 gene encoding a POZ/AT-hook/Kruppel zinc finger (PATZ) transcription factor, is considered a cancer-related gene because of its loss or misexpression in human neoplasias. As for other POZ/domain and Kruppel zinc finger (POK) family members, the transcriptional activity of PATZ is due to the POZ-mediated oligomer formation, suggesting that it might be not a typical transactivator but an architectural transcription factor, thus functioning either as activator or as repressor depending on the presence of proteins able to interact with it. Therefore, to better elucidate PATZ function, we searched for its molecular partners. By yeast two-hybrid screenings, we found a specific interaction between PATZ and BCL6, a human oncogene that plays a key role in germinal center (GC) derived neoplasias. We demonstrate that PATZ and BCL6 interact in germinal center-derived B lymphoma cells, through the POZ domain of PATZ. Moreover, we show that PATZ is able to bind the BCL6 regulatory region, where BCL6 itself acts as a negative regulator, and to contribute to negatively modulate its activity. Consistently, disruption of one or both Patz1 alleles in mice causes focal expansion of thymus B cells, in which BCL6 is up-regulated. This phenotype was almost completely rescued by crossing Patz1(+/-) with Bcl6(+/-) mice, indicating a key role for Bcl6 expression in its development. Finally, a significant number of Patz1 knock-out mice (both heterozygous and homozygous) also develop BCL6-expressing lymphomas. Therefore, the disruption of one or both Patz1 alleles may favor lymphomagenesis by activating the BCL6 pathway

The PALM Technique: histological findings of masked phototherapeutic keratectomy on rabbit corneas

BACKGROUND: To compare the corneal healing response between conventional and phototherapeutic keratectomy through a masking agent, in rabbit corneas. METHODS: 24 adult rabbits underwent phototherapeutic keratectomy. Animals were divided in two groups: 12 received photoablation through a masking agent (PALM gel) and the remaining 12 received conventional phototherapeutic keratectomy of equal depth and served as control. Light and transmission electron microscopy was performed in specimens of both groups obtained: immediately after, four hours, one week, one, three and six months after treatment. RESULTS: Reepitheliazation was complete within five days in all eyes. Light and transmission electron microscopy did not reveal any differences of the healing process in the experimental eyes compared to the controls. CONCLUSION: Photoablation through the PALM technique did not result any evident alterations of the reepithelisation and stromal healing process

Behavior Discovery and Alignment of Articulated Object Classes from Unstructured Video

We propose an automatic system for organizing the content of a collection of unstructured videos of an articulated object class (e.g. tiger, horse). By exploiting the recurring motion patterns of the class across videos, our system: 1) identifies its characteristic behaviors; and 2) recovers pixel-to-pixel alignments across different instances. Our system can be useful for organizing video collections for indexing and retrieval. Moreover, it can be a platform for learning the appearance or behaviors of object classes from Internet video. Traditional supervised techniques cannot exploit this wealth of data directly, as they require a large amount of time-consuming manual annotations. The behavior discovery stage generates temporal video intervals, each automatically trimmed to one instance of the discovered behavior, clustered by type. It relies on our novel motion representation for articulated motion based on the displacement of ordered pairs of trajectories (PoTs). The alignment stage aligns hundreds of instances of the class to a great accuracy despite considerable appearance variations (e.g. an adult tiger and a cub). It uses a flexible Thin Plate Spline deformation model that can vary through time. We carefully evaluate each step of our system on a new, fully annotated dataset. On behavior discovery, we outperform the state-of-the-art Improved DTF descriptor. On spatial alignment, we outperform the popular SIFT Flow algorithm.Comment: 19 pages, 19 figure, 3 tables. arXiv admin note: substantial text overlap with arXiv:1411.788

ANCA-associated vasculitis.

The anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAVs) are a group of disorders involving severe, systemic, small-vessel vasculitis and are characterized by the development of autoantibodies to the neutrophil proteins leukocyte proteinase 3 (PR3-ANCA) or myeloperoxidase (MPO-ANCA). The three AAV subgroups, namely granulomatosis with polyangiitis (GPA), microscopic polyangiitis and eosinophilic GPA (EGPA), are defined according to clinical features. However, genetic and other clinical findings suggest that these clinical syndromes may be better classified as PR3-positive AAV (PR3-AAV), MPO-positive AAV (MPO-AAV) and, for EGPA, by the presence or absence of ANCA (ANCA+ or ANCA-, respectively). Although any tissue can be involved in AAV, the upper and lower respiratory tract and kidneys are most commonly and severely affected. AAVs have a complex and unique pathogenesis, with evidence for a loss of tolerance to neutrophil proteins, which leads to ANCA-mediated neutrophil activation, recruitment and injury, with effector T cells also involved. Without therapy, prognosis is poor but treatments, typically immunosuppressants, have improved survival, albeit with considerable morbidity from glucocorticoids and other immunosuppressive medications. Current challenges include improving the measures of disease activity and risk of relapse, uncertainty about optimal therapy duration and a need for targeted therapies with fewer adverse effects. Meeting these challenges requires a more detailed knowledge of the fundamental biology of AAV as well as cooperative international research and clinical trials with meaningful input from patients