Germplasm collection and ethnobotany of taro (Colocasia esculenta L. Schott) from nineteen districts in the Ashanti, Eastern and Western regions of Ghana

Germplasm collection and ethnobotanical documentation are necessary for effective conservation and management of plant genetic resources. Taro (Colocasia esculenta L. Schott) is one of the staple root and tuber crops in Ghana. The study reports the germplasm and ethnobotanical information of taro collected from 19 districts in the Ashanti, Eastern and Western regions of Ghana. A germplasm collection expedition was undertaken in 58 towns in the districts. Fifty donors were interviewed on the ethnobotany of taro, using a questionnaire based on International Plant Genetic Resources Institute (IPGRI) descriptors for taro. Sixty taro accessions were collected from fields (34), home gardens (23), roadside stalls (2) and the wild (1). Respondents comprised of 27 males and 23 females. (62%). According to respondents of the survey, taro is used for food (100%), animal feed (44%) and folk medicine (4%). The corms (100%) and leaves (64%) are the parts of the plant used. The crop is grown mainly on a small scale for subsistent use by 70 percent of the respondents. Taro leaf blight (TLB) and lack of planting materials were the main constraints to large scale production. Respondents perceived the outbreak of TLB was due to the use of agrochemicals in farming practices in recent times (80%), irradiation (26%) and mythical reasons (10%). There is the need to educate taro growers on the causes and management of taro leaf blight

T Cell Responses to the RTS,S/AS01E and RTS,S/AS02D Malaria Candidate Vaccines Administered According to Different Schedules to Ghanaian Children

BACKGROUND: The Plasmodium falciparum pre-erythrocytic stage candidate vaccine RTS,S is being developed for protection of young children against malaria in sub-Saharan Africa. RTS,S formulated with the liposome based adjuvant AS01(E) or the oil-in-water based adjuvant AS02(D) induces P. falciparum circumsporozoite (CSP) antigen-specific antibody and T cell responses which have been associated with protection in the experimental malaria challenge model in adults. METHODS: This study was designed to evaluate the safety and immunogenicity induced over a 19 month period by three vaccination schedules (0,1-, 0,1,2- and 0,1,7-month) of RTS,S/AS01(E) and RTS,S/AS02(D) in children aged 5-17 months in two research centers in Ghana. Control Rabies vaccine using the 0,1,2-month schedule was used in one of two study sites. RESULTS: Whole blood antigen stimulation followed by intra-cellular cytokine staining showed RTS,S/AS01(E) induced CSP specific CD4 T cells producing IL-2, TNF-α, and IFN-γ. Higher T cell responses were induced by a 0,1,7-month immunization schedule as compared with a 0,1- or 0,1,2-month schedule. RTS,S/AS01(E) induced higher CD4 T cell responses as compared to RTS,S/AS02(D) when given on a 0,1,7-month schedule. CONCLUSIONS: These findings support further Phase III evaluation of RTS,S/AS01(E). The role of immune effectors and immunization schedules on vaccine protection are currently under evaluation. TRIAL REGISTRATION: ClinicalTrials.gov NCT00360230

Global wealth disparities drive adherence to COVID-safe pathways in head and neck cancer surgery

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Assessment of vegetation dynamics in Upper East Region of Ghana based on wavelet multi-resolution analysis

Vegetation variation offers significant information for environmental planning, management, sustainability and prompts caution of ecosystem degradation, particularly for the semiarid regions. Normalized difference vegetation index (NDVI) discloses the coverage growth situation, biomass and photosynthesis strength of vegetation and land-cover alterations. Wavelet was used to decompose NDVI time series into subseries at various timescales. This study used a multi-resolution analysis in association with Mann–Kendall and Sen’s Slope at 95% confidence interval to determine the trends in vegetation dynamics at the Upper East Region (UER) of Ghana. GIMMS NDVI3g time series was used to evaluate the performance of the vegetation at seasonal, interannual and intraannual timescale from 1982 to 2015. The results showed that the variability in NDVI in the region is annually significant. At the seasonal level, the whole surface area showed negative vegetation trend. In terms of the intraannual changes, 11.76% of the surface area showed critical patterns. At the interannual scale, results revealed that 4.40% of the surface area demonstrated significant patterns, while 95.60% indicated nonsignificant pattern. Overall, there was negative performance in the vegetation growth from 1982 to 2015. The 16.6% decrease in vegetation dynamics can be attributed to anthropogenic activities. The results from this study would benefit and provide helpful assistance to water resources managers, agricultural and ecological development officers for sustainable planning of UER

Spatiotemporal dynamics of rainfall in Upper East Region of Ghana, West Africa, 1981-2016

There has been an increasing concern about effects of global warming on rainfall. Negative impacts on rainfall affect the environment and socio-economic activities of nations, globally. Rainfall characteristics at the Upper East Region (UER) of Ghana in terms of temporal and spatial variability are investigated from 1981 to 2016 using rainfall data from Ghana Meteorological Agency (GMet) and Climate Hazards Group Infrared Precipitation with Station. Using cumulative residual analysis, Mann–Kendall (MK), Sen’s slope, wavelet transform (WT) and principal component analysis (PCA) tests, the rainfall variability in UER was examined. Results showed that 1981, 1999, 2002 and 2013 were years of major changes in rainfall variation. The MK and Sen’s slope showed that 55.71% of UER have decreasing monthly rainfall with 27.34% significant trend. Months April, May and June showed decreasing rainfall trends. Months July, August and September showed significant increasing rainfall trend. WT revealed a significant variation in the annual rainfall. PCA revealed that the spatial variability of rainfall in UER is very diverse with 33.76% of the variability located in the northeastern part. The findings serve as benchmark in providing in-depth understanding of rainfall variation in UER for water resource managers, agriculturalists and drought mitigation

Diagnosis patterns of sickle cell disease in Ghana: a secondary analysis.

BACKGROUND: Despite having the highest prevalence of sickle cell disease (SCD) in the world, no country in Sub-Saharan Africa has a universal screening program for the disease. We sought to capture the diagnosis patterns of SCD (age at SCD diagnosis, method of SCD diagnosis, and age of first pain crisis) in Accra, Ghana. METHODS: We administered an in-person, voluntary survey to parents of offspring with SCD between 2009 and 2013 in Accra as a part of a larger study and conducted a secondary data analysis to determine diagnosis patterns. This was conducted at a single site: a large academic medical center in the region. Univariate analyses were performed on diagnosis patterns; bivariate analyses were conducted to determine whether patterns differed by participant’s age (children: those  = 18 years old whose parents completed a survey about them), or their disease severity based on SCD genotype. Pearson’s chi-squared were calculated. RESULTS: Data was collected on 354 unique participants from parents. Few were diagnosed via SCD testing in the newborn period. Only 44% were diagnosed with SCD by age four; 46% had experienced a pain crisis by the same age. Most (66%) were diagnosed during pain crisis, either in acute (49%) or primary care (17%) settings. Children were diagnosed with SCD at an earlier age (74% by four years old); among the adults, parents reflected that 30% were diagnosed by four years old (p < 0.001). Half with severe forms of SCD were diagnosed by age four, compared to 31% with mild forms of the disease (p = 0.009). CONCLUSIONS: The lack of a robust newborn screening program for SCD in Accra, Ghana, leaves children at risk for disease complications and death. People in our sample were diagnosed with SCD in the acute care setting, and in their toddler or school-age years or thereafter, meaning they are likely being excluded from important preventive care. Understanding current SCD diagnosis patterns in the region can inform efforts to improve the timeliness of SCD diagnosis, and improve the mortality and morbidity caused by the disease in this high prevalence population