281 research outputs found
Does GDNF exert its neuroprotective effects on photoreceptors in the rd1 retina through the glial glutamate transporter GLAST?
PURPOSE: We previously demonstrated that exogenous glial cell line-derived neurotrophic factor (GDNF) induces histological and functional protection of photoreceptors in the retinal degeneration (rd1) mouse model. The mechanisms underlying such neuroprotection remain elusive. In parallel to this work, we provided evidence for the occurrence of glutamate-mediated excitotoxic phenomena contributing to rod photoreceptor death in the rd1 retina in the companion paper. In the present study, we investigated whether, as demonstrated in other models, GDNF could exert its neuroprotective effect on photoreceptors through Muller glial cells (MGC) by promoting the expression of the glial L-glutamate/L-aspartate transporter (GLAST), an endogenous neuroprotective mechanism against glutamate-mediated excitotoxicity. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to compare the mRNA expression levels of GDNF receptors between rd1 and wild-type mouse retinas as well as between MGC and mixed retinal cell cultures. Recombinant GDNF was applied to pure MGC cultures, to rd1 retinal organ cultures and injected subretinally into rd1 mouse eyes. GLAST expression following GDNF treatment was measured by RT-PCR, immunoblotting and immunohistochemistry. Free glutamate and glutamine levels were quantified in rd1 retinas after GDNF or control treatment using an amino acid analyzer. RESULTS: mRNA expression studies of GDNF receptors, GFRalpha-1 and Ret, demonstrated that GDNF receptors were not exclusively expressed by the degenerating photoreceptor cells but mainly by MGC. Exogenous GDNF application to MGC cultures, rd1 mouse retinal explants and in vivo rd1 mouse retinas increased the expression of GLAST by 48% in retinal explants (p<0.005) and by 25% in vivo (p<0.0005). GLAST protein expression in MGC was particularly increased around degenerative photoreceptors. Free glutamate and glutamine levels in the rd1 retina were not significantly modified by exogenous GDNF. CONCLUSIONS: Our data suggest that, in the rd1 mouse retina, GDNF neuroprotective effect on photoreceptors can be mediated indirectly through the activation of MGC. We demonstrate that injection of recombinant GDNF enhances the expression of GLAST and more particularly around the degenerating photoreceptors. Since we failed to demonstrate that GDNF decreases free glutamate levels, we could not ascertain whether GDNF promoted photoreceptor-survival via an increase of glutamate uptake and, therefore, a change in glutamate distributio
Forêts et planification foncière en région méridionale
Constatant une importante utilisation de terres agricoles de qualité par l'urbanisation, l'auteur propose une redéfinition des sols à protéger et en particulier une précision dans la définition des sols forestiers
Evaluation of an indirect method for leaf area index determination in the vineyard: Combined effects of cultivar, year and training system
The performance of the plant canopy analyzer LAI-2000 was evaluated in three seasons to estimate vineyard LAI (leafarea index) in the Bordeaux area. Measurements were made in a vertical-trellised vineyard with 5555 Semillon and Sauvignon blanc vines per ha, and with 2525 lyre-trained Cabernet franc vines per ha. Various LAI-2000 sampling protocols were tested and the data compared with destructive determinations. While the results from single vines a ere not satisfactory, very good relationships were obtained if five consecutive vines were used. In the vertical-trellised vineyard, a very accurate and direct estimate of LAI could be obtained with the LAI-2000 using a combination of "parallel" and "diagonal" sampling protocol. LAI values obtained by direct and LAI-2000 had a 1:1 correspondence and were identical for both, year and cultivar. For the lyre system, a local calibration was required, mainly because of the contribution of shoots, perennial vine parts and posts. This calibration was easily obtained by progressively removing leaves from the vines
Selective Reflection Spectroscopy on the UV Third Resonance Line of Cs : Simultaneous Probing of a van der Waals Atom-Surface Interaction Sensitive to Far IR Couplings and of Interatomic Collisions
We report on the analysis of FM selective reflection experiments on the
6S1/2->8P3/2 transition of Cs at 388 nm, and on the measurement of the surface
van der Waals interaction exerted by a sapphire interface on Cs(8P3/2). Various
improvements in the systematic fitting of the experiments have permitted to
supersede the major difficulty of a severe overlap of the hyperfine components,
originating on the one hand in a relatively small natural structure, and on the
other hand on a large pressure broadening imposed by the high atomic density
needed for the observation of selective reflection on a weak transition. The
strength of the van der Waals surface interaction is evaluated to be 7310
kHz.m3. An evaluation of the pressure shift of the transition is also
provided as a by-product of the measurement. We finally discuss the
significance of an apparent disagreement between the experimental measurement
of the surface interaction, and the theoretical value calculated for an
electromagnetic vacuum at a null temperature. The possible influence of the
thermal excitation of the surface is evoked, because, the dominant
contributions to the vW interaction for Cs(8P3/2) lie in the far infrared
range.Comment: submitted to Laser Physics - issue in the memory of Herbert Walther
Nitrogen cost minimization is promoted by structural changes in the transcriptome of N-deprived Prochlorococcus cells
Prochlorococcus is a globally abundant marine cyanobacterium with many adaptations that reduce cellular nutrient requirements, facilitating growth in its nutrient-poor environment. One such genomic adaptation is the preferential utilization of amino acids containing fewer N-atoms, which minimizes cellular nitrogen requirements. We predicted that transcriptional regulation might further reduce cellular N budgets during transient N limitation. To explore this, we compared transcription start sites (TSSs) in Prochlorococcus MED4 under N-deprived and N-replete conditions. Of 64 genes with primary and internal TSSs in both conditions, N-deprived cells initiated transcription downstream of primary TSSs more frequently than N-replete cells. Additionally, 117 genes with only an internal TSS demonstrated increased internal transcription under N-deprivation. These shortened transcripts encode predicted proteins with an average of 21% less N content compared to full-length transcripts. We hypothesized that low translation rates, which afford greater control over protein abundances, would be beneficial to relatively slow-growing organisms like Prochlorococcus. Consistent with this idea, we found that Prochlorococcus exhibits greater usage of glycine-glycine motifs, which causes translational pausing, when compared to faster growing microbes. Our findings indicate that structural changes occur within the Prochlorococcus MED4 transcriptome during N-deprivation, potentially altering the size and structure of proteins expressed under nutrient limitation.Gordon and Betty Moore Foundation (Grant GBMF495)Simons Foundation (Award 329108)National Science Foundation (U.S.) (Grant DBI-0424599
Plaidoyer pour une sylviculture du Pin d'Alep par les techniciens de terrain
Constatant qu'ils gèrent une forêt où le Pin d'Alep tient une place prédominante, les auteurs proposent d'en améliorer la sylviculture. Ils évoquent les modes de traitement qu'impose l'état des peuplements : la futaie régulière devant être traitée en priorité, la futaie d'aspect jardiné et les peuplements mélangés qui sont les plus délicats à aménager. Après avoir examiné les difficultés rencontrées dans ces aménagements, les auteurs concluent que le mode de sylviculture le plus approprié est la futaie régulière par parquets de 0,5 à 4 ha
Organic waste as a sustainable feedstock for platform chemicals
Biorefineries have been established since the 1980s for biofuel production, and there has been a switch lately from first to second generation feedstocks in order to avoid the food versus fuel dilemma. To a lesser extent, many opportunities have been investigated for producing chemicals from biomass using by-products of the present biorefineries, simple waste streams. Current facilities apply intensive pre-treatments to deal with single substrate types such as carbohydrates. However, most organic streams such as municipal solid waste or algal blooms present a high complexity and variable mixture of molecules, which makes specific compound production and separation difficult. Here we focus on flexible anaerobic fermentation and hydrothermal processes that can treat complex biomass as a whole to obtain a range of products within an integrated biorefinery concept
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