Molecular epidemiology and nitrofurantoin resistance determinants of nitrofurantoin-non-susceptible Escherichia coli isolated from urinary tract infections

Objectives: The worldwide emergence of multidrug-resistant uropathogens has resulted in the revival of old antibiotics such as nitrofurantoin (NIT) for the treatment of uncomplicated urinary tract infections (UTIs). This study aimed to identify determinants of NIT resistance and to investigate the genetic diversity of NIT-resistant (NIT-R) Escherichia coli isolates. Methods: Six NIT-R and three NIT-susceptible clinical E. coli isolates from patients with UTI were studied. The susceptibility of the isolates to various classes of antibiotics was evaluated by disk diffusion. The presence of plasmid-encoded efflux pump genes (oqxA and oqxB) was investigated by PCR. Nucleotide sequences of the nfsA, nfsB and ribE genes were determined. The genetic relatedness of the NIT-R isolates was evaluated by multilocus sequence typing (MLST). Results: All six NIT-R isolates were characterised with high-level NIT resistance (MIC � 512 mg/L) and they belonged to five distinct STs including ST131 (n = 2), ST73, ST405, ST10 and ST354 (n = 1 each). Amikacin, carbapenems, minocycline, tigecycline and fosfomycin were the most active agents against the studied uropathogens. The oqxA and oqxB genes were not detected in any isolate. All NIT-R isolates harboured inactivating genetic alterations in nfsA and nfsB NfsA H11Y, S33N, S38Y, W212R substitutions, �g638 (frameshift), �a64-g73 (frameshift) and NfsB F84S, P45S, W94Stop, E197Stop substitutions, �nfsB locus. The ribE gene of most isolates was unaffected, except for one isolate co-harbouring a deleterious RibE G85C substitution and NfsA/B alterations. Conclusion: NIT resistance in the studied E. coli isolates was mainly mediated by nfsA and nfsB alterations. © 201

Nanoparticles charge response from electrostatic force microscopy

Electrostatic force microscopy (EFM) allows measurement of tiny changes in tip-sample capacitance. When nanoobjects are studied by EFM, they only contribute a very small fraction of the total capacitance between the tip and the sample. We show that the analysis of 3D maps of the EFM signal allows extracting the contribution of the nanomaterial to the total capacitance. This opens the way to applications of EFM as a measure of the dielectric coefficient of electrically insulating nanomaterials or the quantum capacitance of conducting nanomaterials. We apply this method to study the charge response of magnetite, Fe3O4, nanoparticles. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4790587

Room-temperature nine-µm-wavelength photodetectors and GHz-frequency heterodyne receivers

Room-temperature operation is essential for any optoelectronics technology that aims to provide low-cost, compact systems for widespread applications. A recent technological advance in this direction is bolometric detection for thermal imaging¹, which has achieved relatively high sensitivity and video rates (about 60 hertz) at room temperature. However, owing to thermally induced dark current, room-temperature operation is still a great challenge for semiconductor photodetectors targeting the wavelength band between 8 and 12 micrometres², and all relevant applications, such as imaging, environmental remote sensing and laser-based free-space communication³,⁴,⁵, have been realized at low temperatures. For these devices, high sensitivity and high speed have never been compatible with high-temperature operation⁶,⁷. Here we show that a long-wavelength (nine micrometres) infrared quantum-well photodetector⁸ fabricated from a metamaterial made of sub-wavelength metallic resonators⁹,¹⁰,¹¹,¹² exhibits strongly enhanced performance with respect to the state of the art up to room temperature. This occurs because the photonic collection area of each resonator is much larger than its electrical area, thus substantially reducing the dark current of the device¹³. Furthermore, we show that our photonic architecture overcomes intrinsic limitations of the material, such as the drop of the electronic drift velocity with temperature¹⁴,¹⁵, which constrains conventional geometries at cryogenic operation⁶. Finally, the reduced physical area of the device and its increased responsivity allow us to take advantage of the intrinsic high-frequency response of the quantum detector⁷ at room temperature. By mixing the frequencies of two quantum-cascade lasers¹⁶ on the detector, which acts as a heterodyne receiver, we have measured a high-frequency signal, above four gigahertz (GHz). Therefore, these wide-band uncooled detectors could benefit technologies such as high-speed (gigabits per second) multichannel coherent data transfer¹⁷ and high-precision molecular spectroscopy¹⁸

Effect of COVID-19 medications on corrected QT interval and induction of torsade de pointes: Results of a multicenter national survey

Background: There are some data showing that repurposed drugs used for the Coronavirus disease-19 (COVID-19) have potential to increase the risk of QTc prolongation and torsade de pointes (TdP), and these arrhythmic side effects have not been adequately addressed in COVID-19 patients treated with these repurposed medications. Methods: This is the prospective study of 2403 patients hospitalised at 13 hospitals within the COVID-19 epicentres of the Iran. These patients were treated with chloroquine, hydroxychloroquine, lopinavir/ritonavir, atazanavir/ritonavir, oseltamivir, favipiravir and remdesivir alone or in combination with azithromycin. The primary outcome of the study was incidence of critical QTc prolongation, and secondary outcomes were incidences of TdP and death. Results: Of the 2403 patients, 2365 met inclusion criteria. The primary outcome of QTc � 500 ms and �QTc � 60 ms was observed in 11.2 and 17.6 of the patients, respectively. The secondary outcomes of TdP and death were reported in 0.38 and 9.8 of the patients, respectively. The risk of critical QT prolongation increased in the presence of female gender, history of heart failure, treatment with hydroxychloroquine, azithromycin combination therapy, simultaneous furosemide or beta-blocker therapy and acute renal or hepatic dysfunction. However, the risk of TdP was predicted by treatment with lopinavir-ritonavir, simultaneous amiodarone or furosemide administration and hypokalaemia during treatment. Conclusion: This cohort showed significant QTc prolongation with all COVID-19 medications studied, however, life-threatening arrhythmia of TdP occurred rarely. Among the repurposed drugs studied, hydroxychloroquine or lopinavir-ritonavir alone or in combination with azithromycin clearly demonstrated to increase the risk of critical QT prolongation and/or TdP. © 2021 John Wiley & Sons Ltd

Design of a Pilot SOFC System for the Combined Production of Hydrogen and Electricity under Refueling Station Requirements

The objective of the current work is to support the design of a pilot hydrogen and electricity producing plant that uses natural gas (or biomethane) as raw material, as a transition option towards a 100% renewable transportation system. The plant, with a solid oxide fuel cell (SOFC) as principal technology, is intended to be the main unit of an electric vehicle station. The refueling station has to work at different operation periods characterized by the hydrogen demand and the electricity needed for supply and self-consumption. The same set of heat exchangers has to satisfy the heating and cooling needs of the different operation periods. In order to optimize the operating variables of the pilot plant and to provide the best heat exchanger network, the applied methodology follows a systematic procedure for multi-objective, i.e. maximum plant efficiency and minimum number of heat exchanger matches, and multi-period optimization. The solving strategy combines process flow modeling in steady state, superstructure-based mathematical programming and the use of an evolutionary-based algorithm for optimization. The results show that the plant can reach a daily weighted efficiency exceeding 60%, up to 80% when considering heat utilization

5-ps-long terahertz pulses from an active-mode-locked quantum cascade laser

A significant research effort toward the generation of short terahertz (THz) pulses using quantum cascade lasers (QCLs) has been undertaken over the past few years. This is motivated by the desire to realize compact, electrically driven THz pulsed sources to study the dynamics of a variety of solid-state systems using ultrafast spectroscopy techniques, or to replace standard THz time domain spectroscopy systems for real-life applications. In this work we demonstrate the generation of 5-ps-long, transform-limited pulses by actively mode locking a 2.5 THz QCL. The pulse duration is a factor of 2 shorter than what has been reported to date with this technique, and has been achieved thanks to the use of a metal–metal waveguide, which favors lasing over a broader spectral bandwidth compared to previously employed single-plasmon waveguides. Active mode locking is obtained using a low-power radio-frequency (RF) signal to modulate the QCL current at the cavity round-trip frequency. The modulation does not affect the QCL emission spectrum. This is in striking contrast with previous reports of active mode locking where multimode emission was the direct consequence of the RF modulation

GHz heterodyne generation using Two DFB Mid-IR QCL lasers on a 9μm QWIP

We report the implementation of a heterodyne measurement setup using two DFB Mid-IR QCL lasers on a 9µm Quantum Well Infrared Photodetector (QWIP). We present the measurement of the signal-to-noise ratio characterization of the detector thanks to the heterodyne technique and the obtained frequency stability for the QCL in terms of a linewidth inferior to 3MHz at 300 KHz of resolution bandwidth