59 research outputs found
Effectiveness of Bradyrhizobium japonicum inoculation on nodulation dynamics in Glycine max (L.) roots
Bradyrhizobium japonicum bacteria on the intensity of nodulation during nine phenophases (R1-R7) in relation to the isoflavone content in soybean seeds. Nine domestic soybean varieties were sown in three replicates, with Bradyrhizobium japonicum inoculated and non-inoculated seeds. The different morphological characteristics of nodules, the number and mass of nodules were studied during plants’ ontogenesis. The content of individual isoflavones in the sowing seed material was determined using high performance liquid chromatography mass spectrometry (HPLC-MS). The most abundant isoflavone was acetylgenistin in the variety Galeb (2741.4 μg/g), and the highest content of all isoflavones was recorded in the variety Gorštak (8117.7 μg/g). The variety Sava of the inoculated treatment exhibited the highest average values of the nodule mass (21.1 mg) and the highest number of nodules (23.3) in the phenophase, in which 10% of pods reached full length (R4). The average values of mass and number of nodules were calculated for the entire vegetation period, being higher in the inoculated treatment. There was no positive correlation between the content of isoflavones in seeds and the number and mass of nodules. The impact of quantity of isoflavone on nodulation intensity in soybeans was not significant,, which could be related to already saturated soil with nitrogen-fixing bacteria
LC–DAD–MS phenolic characterisation of six invasive plant species in Croatia and determination of their antimicrobial and cytotoxic activity
Invasive plants’ phytochemicals are important for their invasiveness, enabling them to
spread in new environments. However, these chemicals could offer many pharmaceutical compounds
or active ingredients for herbal preparations. This study provides the first LC–MS phytochemical
screening of six invasive alien plant species (IAPS) in the Istria region (Croatia): Ailanthus altissima,
Ambrosia artemisiifolia, Conyza canadensis, Dittrichia viscosa, Erigeron annuus, and Xanthium strumarium.
The study aims to identify and quantify the phenolic content of their leaf extracts and assess their
antimicrobial and cytotoxic potential. A total of 32 species-specific compounds were recorded.
Neochlorogenic, chlorogenic, and 5-p-coumaroylquinic acids, quercetin-3-glucoside, and kaempferol
hexoside were detected in all the tested IAPS. Hydroxycinnamic acid derivatives were the main
components in all the tested IAPS, except in E. annuus, where flavanones dominated with a share of
70%. X. strumarium extract had the best activity against the tested bacteria, with an average MIC value
of 0.11 mg/mL, while A. altissima and X. strumarium extracts had the best activity against the tested
fungi, with an average MIC value of 0.21 mg/mL in both cases. All the plant extracts studied, except X.
strumarium, were less cytotoxic than the positive control. The results provided additional information
on the phytochemical properties of IAPS and their potential for use as antimicrobial agents.SUPPLEMENTARY MATERIALS : Figure S1: Heat map presenting the representations of phenolic groups in different invasive plants; Figure S2: Chromatogram of the acetone extracts of the plant species leaves developed in ethyl acetate/methanol/water (EMW) solvent system sprayed with vanillin– sulphuric acid and TLC bioautograms; Table S1: Spectrum, mass-to-charge ratio (m/z) values of the molecular masses, and main fragments (MS2—second-generation product ion, MS3—thirdgeneration product ion) in negative ion mode ((M-H)−) identified with ESI–MS and the distribution of individual compounds in different invasive plants.The Croatian Science Foundation, the Slovenian Research Agency (ARRS) and the University of Pretoria, South Africa.https://www.mdpi.com/journal/plantsdm2022Paraclinical Science
Carbohydrate, phenolic and antioxidant level in relation to chlorophyll a content in oilseed winter rape (Brassica napus L.) inoculated with Leptosphaeria maculans
Syftet med föreliggande studien var att undersöka om sjuksköterskors egna rökvanor påverkar attityden till tobakspreventivt arbete på sjukhuset, både till tobakspreventivt arbete med patienterna och attityden till rökfritt sjukhus. Studien är empirisk och utfördes genom kvalitativa intervjuer med sex sjuksköterskor på en vårdavdelning på ett sjukhus i södra Sverige. Data från intervjuerna analyserades och resulterade i sju olika teman: Preventiva rollen, Kunskap om prevention, Vem skall leda det preventiva arbetet, Rökkontroll, Utbildningsnivå och rökning, Sjuksköterskan, en förebild?, Vem ska hjälpa patienten vid rökstopp på sjukhuset?, Är det någon skillnad mellan icke rökande och rökande vad avser rökpreventionen?. Den preventiva rollen hamnade i fokus och skillnader fanns mellan rökande och icke rökande sjuksköterskor både vad gäller preventivt omvårdnasarbete och kontrollThe aim of the present study is to investigate whether nurses smoking habits influence their attitude to tobacco prevention in hospitals, both in their work with patients and regarding their attitude to hospital smoking bans. The following question was posed: is there a difference between smoking and non-smoking nurses in patient-care activities regarding smoking prevention and control? The study is qualitative, based on qualitative interviews with six nurses at a ward of a hospital in Sweden. Interview data were analyzed and eight themes emerged: the role in prevention work knowledge of prevention who is to lead prevention work smoking control smoking and education levels the nurse as a role model who is to help the patient give up smoking possible differences between nonsmoking and smoking nurses regarding smoking prevention. The role in prevention work turned out to be central. Smoking nurses had greater difficulties in connection with preventive work and control, due to their personal experience of how hard it can be to give up smoking habits
Does plant growth and yield affected by Prohexadione Ca cause changes in chemical fruit composition of 'Loch Ness' and 'Triple Crown' blackberries?
Two semi-erect blackberry cultivars, 'Loch Ness' and 'Triple Crown', were used to investigate the effect of Prohexadione-Ca (ProCa) on vegetative growth, generative potential and fruit quality parameters. ProCa reduced the cane and internode length, but increased the node number per meter of cane length in both cultivars. ProCa application enhanced the number of fruiting branches and yield per bush in the following year. Fruit weight, number of drupelets per fruit, fruit shape index, content of soluble solids, shikimic acid and some phenolics were significantly increased by ProCa. ProCa-cultivar interaction was significant regarding the content of quercetin-3-rutino-side and procyanidin trimer. 'Loch Ness' had approximately 40% higher yields per bush and contained abundant quantities of anthocyanins and flavonols, whereas larger fruits and higher content of primary metabolites, some flavanols and total hydroxycinnamic acid derivatives were found in 'Triple Crown'. According to obtained results, the ProCa application at the rate from 100 to 200 mg L-1 is able to establish acceptable growth control without negative effects on yield and chemical fruit composition
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