The colourful antenna subtraction method

We present a general subtraction scheme for NNLO calculations in massless QCD: the colourful antenna subtraction method. It is a reformulation of the antenna subtraction approach designed to address some of the limitations of the traditional framework, especially aiming at high-multiplicity processes. In the context of the new formalism, structures needed to locally subtract the infrared-divergent behaviour of real emission corrections are systematically inferred from virtual subtraction terms, relying on the cancellation of infrared singularities and on the correspondence between integrated and unintegrated antenna functions. We illustrate in detail how the colourful antenna subtraction method works up to NNLO. The algorithm is particularly suited to be fully automated for the generation of NNLO subtraction terms for generic processes. We employ the new formalism to assemble the subtraction terms required for the calculation of the NNLO correction to hadronic three-jet production and describe their validation procedure

The colourful antenna subtraction method

We present a general subtraction scheme for NNLO calculations in massless QCD: the \textit{colourful antenna subtraction method}. It is a reformulation of the antenna subtraction approach designed to address some of the limitations of the traditional framework, especially aiming at high-multiplicity processes. In the context of the new formalism, structures needed to locally subtract the infrared-divergent behaviour of real emission corrections are systematically inferred from virtual subtraction terms, relying on the cancellation of infrared singularities and on the correspondence between integrated and unintegrated antenna functions. We illustrate in detail how the colourful antenna subtraction method works up to NNLO. The algorithm is particularly suited to be fully automated for the generation of NNLO subtraction terms for generic processes. We employ the new formalism to assemble the subtraction terms required for the calculation of the NNLO correction to hadronic three-jet production and describe their validation procedure

Automation of antenna subtraction in colour space: gluonic processes

We present the colourful antenna subtraction method, a reformulation of the antenna subtraction scheme for next-to-next-to-leading order (NNLO) calculations in QCD. The aim of this new approach is to achieve a general and process-independent construction of the subtraction infrastructure at NNLO. We rely on the predictability of the infrared singularity structure of one- and two-loop amplitudes in colour space to generate virtual subtraction terms and, subsequently, we define an automatable procedure to systematically infer the expression of the real subtraction terms, guided by the correspondence between unintegrated and integrated antenna functions. To demonstrate the applicability of the described approach, we compute the full colour NNLO correction to gluonic three-jet production pp(gg)→ggg, in the gluons-only assumption

Automation of antenna subtraction in colour space: gluonic processes

We present the colourful antenna subtraction method, a reformulation of the antenna subtraction scheme for next-to-next-to-leading order (NNLO) calculations in QCD. The aim of this new approach is to achieve a general and process-independent construction of the subtraction infrastructure at NNLO. We rely on the predictability of the infrared singularity structure of one- and two-loop amplitudes in colour space to generate virtual subtraction terms and, subsequently, we define an automatable procedure to systematically infer the expression of the real subtraction terms, guided by the correspondence between unintegrated and integrated antenna functions. To demonstrate the applicability of the described approach, we compute the full colour NNLO correction to gluonic three-jet production pp(gg) → ggg, in the gluons-only assumption

Automation of antenna subtraction in colour space: gluonic processes

We present the colourful antenna subtraction method, a reformulation of the antenna subtraction scheme for next-to-next-to-leading order (NNLO) calculations in QCD. The aim of this new approach is to achieve a general and process-independent construction of the subtraction infrastructure at NNLO. We rely on the predictability of the infrared singularity structure of one- and two-loop amplitudes in colour space to generate virtual subtraction terms and, subsequently, we define an automatable procedure to systematically infer the expression of the real subtraction terms, guided by the correspondence between unintegrated and integrated antenna functions. To demonstrate the applicability of the described approach, we compute the full colour NNLO correction to gluonic three-jet production pp(gg) → ggg, in the gluons-only assumption

Heterodimer of A2A and Oxytocin Receptors Regulating Glutamate Release in Adult Striatal Astrocytes

Background: Roles of astrocytes in the modulatory effects of oxytocin (OT) in central nervous system are increasingly considered. Nevertheless, OT effects on gliotransmitter release have been neglected. Methods: In purified astrocyte processes from adult rat striatum, we assessed OT receptor (OTR) and adenosine A2A receptor expression by confocal analysis. The effects of receptors activation on glutamate release from the processes were evaluated; A2A-OTR heteromerization was assessed by co-immunoprecipitation and PLA. Structure of the possible heterodimer of A2A and OT receptors was estimated by a bioinformatic approach. Results: Both A2A and OT receptors were expressed on the same astrocyte processes. Evidence for A2A-OTR receptor-receptor interaction was obtained by measuring the release of glutamate: OT inhibited the evoked glutamate release, while activation of A2A receptors, per se ineffective, abolished the OT effect. Biochemical and bio-physical evidence for A2A-OTR heterodimers on striatal astrocytes was also obtained. The residues in the transmembrane domains 4 and 5 of both receptors are predicted to be mainly involved in the heteromerization. Conclusions: When considering effects of OT in striatum, modulation of glutamate release from the astrocyte processes and of glutamatergic synapse functioning, and the interaction with A2A receptors on the astrocyte processes should be taken into consideration

Virtual QCD corrections to gluon-initiated diphoton plus jet production at hadron colliders

We present an analytic computation of the gluon-initiated contribution to diphoton plus jet production at hadron colliders up to two loops in QCD. We reconstruct the analytic form of the finite remainders from numerical evaluations over finite fields including all colour contributions. Compact expressions are found using the pentagon function basis. We provide a fast and stable implementation for the colour- and helicity-summed interference between the one-loop and two-loop finite remainders in C++ as part of the NJet library

Flow synthesis and biological studies of an analgesic adamantane derivative that inhibits P2X7-evoked glutamate release

We report the biological evaluation of a class of adamantane derivatives, which were achieved via modified telescoped machine-assisted flow procedure. Among the series of compounds tested in this work, 5 demonstrated outstanding analgesic properties. This compound showed that its action was not mediated through direct interaction with opioid and/or cannabinoid receptors. Moreover, it did not display any significant anti-inflammatory properties. Experiments carried out on rat cerebrocortical purified synaptosomes indicated that 5 inhibits the P2X7-evoked glutamate release, which may contribute to its antinociceptive properties. Nevertheless, further experiments are ongoing to characterize the pharmacological properties and mechanism of action of this molecule

Vesicular glutamate release from feeder-free hiPSC-derived neurons

Human-induced pluripotent stem cells (hiPSCs) represent one of the main and powerful tools for the in vitro modeling of neurological diseases. Standard hiPSC-based protocols make use of animal-derived feeder systems to better support the neuronal differentiation process. Despite their efficiency, such protocols may not be appropriate to dissect neuronal specific properties or to avoid interspecies contaminations, hindering their future translation into clinical and drug discovery approaches. In this work, we focused on the optimization of a reproducible protocol in feeder-free conditions able to generate functional glutamatergic neurons. This protocol is based on a generation of neuroprecursor cells differentiated into human neurons with the administration in the culture medium of specific neurotrophins in a Geltrex-coated substrate. We confirmed the efficiency of this protocol through molecular analysis (upregulation of neuronal markers and neurotransmitter receptors assessed by gene expression profiling and expression of the neuronal markers at the protein level), morphological analysis, and immunfluorescence detection of pre-synaptic and post-synaptic markers at synaptic boutons. The hiPSC-derived neurons acquired Ca2+-dependent glutamate release properties as a hallmark of neuronal maturation. In conclusion, our study describes a new methodological approach to achieve feeder-free neuronal differentiation from hiPSC and adds a new tool for functional characterization of hiPSC-derived neurons

Event Generators for High-Energy Physics Experiments

We provide an overview of the status of Monte-Carlo event generators for high-energy particle physics. Guided by the experimental needs and requirements, we highlight areas of active development, and opportunities for future improvements. Particular emphasis is given to physics models and algorithms that are employed across a variety of experiments. These common themes in event generator development lead to a more comprehensive understanding of physics at the highest energies and intensities, and allow models to be tested against a wealth of data that have been accumulated over the past decades. A cohesive approach to event generator development will allow these models to be further improved and systematic uncertainties to be reduced, directly contributing to future experimental success. Event generators are part of a much larger ecosystem of computational tools. They typically involve a number of unknown model parameters that must be tuned to experimental data, while maintaining the integrity of the underlying physics models. Making both these data, and the analyses with which they have been obtained accessible to future users is an essential aspect of open science and data preservation. It ensures the consistency of physics models across a variety of experiments