Phonocardiogram simulator Patent

Phonocardiogram simulator producing electrical voltage waves to control amplitude and duration between simulated sound

Heterologous prion-forming proteins interact to cross-seed aggregation in Saccharomyces cerevisiae

AbstractThe early stages of protein misfolding remain incompletely understood, as most mammalian proteinopathies are only detected after irreversible protein aggregates have formed. Cross-seeding, where one aggregated protein templates the misfolding of a heterologous protein, is one mechanism proposed to stimulate protein aggregation and facilitate disease pathogenesis. Here, we demonstrate the existence of cross-seeding as a crucial step in the formation of the yeast prion [PSI+], formed by the translation termination factor Sup35. We provide evidence for the genetic and physical interaction of the prion protein Rnq1 with Sup35 as a predominant mechanism leading to self-propagating Sup35 aggregation. We identify interacting sites within Rnq1 and Sup35 and determine the effects of breaking and restoring a crucial interaction. Altogether, our results demonstrate that single-residue disruption can drastically reduce the effects of cross-seeding, a finding that has important implications for human protein misfolding disorders.</jats:p

A phonocardiogram simulator

Simulator calibrates and checks out phonocardiograms used in physiological monitoring of astronauts in flight and during flight simulation. It is also used to check out telemetry systems and instrumentation systems for phonocardiogram monitoring in hospitals and medical care centers, and in training personnel to use such systems

The Critical Incident Questionnaire (CIQ): From Research to Practice and Back Again

This paper reviews ways the Critical Incident Questionnaire (CIQ) has been used, adapted, and developed over four years of near continual organizational and academic use. It explores how the tool is implemented, the issues that led to its adaptation, and the current working version. Future research areas are also suggested

Critical HRD—Need for Emergence of Critters in Adult Education

Critters seek to expose, understand, and change the underlying causes of a phenomenon—the business of adult education. Adult education is foundational to the engagement of critical management studies (CMS). Critical HRD appears to be a bridge between CMS frameworks and Adult Education theories. Critters will ensure the complexities and breadth of influences get threaded for all stakeholders

Prion-associated toxicity is rescued by elimination of cotranslational chaperones

The nascent polypeptide-associated complex (NAC) is a highly conserved but poorly characterized triad of proteins that bind near the ribosome exit tunnel. The NAC is the first cotranslational factor to bind to polypeptides and assist with their proper folding. Surprisingly, we found that deletion of NAC subunits in Saccharomyces cerevisiae rescues toxicity associated with the strong [PSI+] prion. This counterintuitive finding can be explained by changes in chaperone balance and distribution whereby the folding of the prion protein is improved and the prion is rendered nontoxic. In particular, the ribosome-associated Hsp70 Ssb is redistributed away from Sup35 prion aggregates to the nascent chains, leading to an array of aggregation phenotypes that can mimic both overexpression and deletion of Ssb. This toxicity rescue demonstrates that chaperone modification can block key steps of the prion life cycle and has exciting implications for potential treatment of many human protein conformational disorders