QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND BACTERICIDAL ACTIVITY OF PELARGONIUM GRAVEOLENS L'HER

Objective: The present study investigates the qualitative and quantitative phytoconstituents and bactericidal effect of medicinally important Pelargonium graveolens L' Her leaves extracts. Methods: Preliminary phytochemical screening analyses were determined using standard protocol methods. In addition, antibacterial activities of the P. graveolens leaves extracts were evaluated by disc diffusion method against Gram-positive and Gram-negative bacteria. Results: The results revealed that ethanolic extract of P. graveolens was found to be the predominant occurrence of phytochemicals (9/11) which contains flavonoid, phenol, tannins, saponins, reducing sugar, glycosides, terpenoids, anthraquinone, and phlorotannins while starch and steroids were absent. In quantitative estimation of bioactive phytoconstituents showed carbohydrates (74 mg/gdw), protein (41.25 mg/gdw), chlorophyll (2.2±0.05 mg/gdw), lipids (0.07 mg/gdw), tannins (135.3 gm/gdw), phenolic compounds (123.75 mg/gdw) and flavonoids (50 mg/gdw) were found to have higher amount in ethanolic extract followed by acetone, methanol and aqueous extract and also showed an inhibitory action on growth of tested bacteria. Conclusion: Ethanolic extract of the P. graveolens leaves hold promises as a potential source of pharmaceutically important phytochemicals and also have strong antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa and Klebsiella pneumonia. Keywords: Phytochemical screening, Primary and secondary metabolites, Antibacterial screening, Pelargonium graveolen

Success stories of model sea-cage farm Sippikulam fishervillage, Thoothukudi district

Success stories of model sea-cage farm Sippikulam fishervillage, Thoothukudi distric

Landings of juvenile Uroteuthis (Photololigo) singhalensis in Tuticorin Fishing Harbour

In Tuticorin Fishing Harbour about 200 trawlers operate daily from 5 am to 11 pm for single day fishing. Wooden and steel trawlers in three sizes, namely small boats (OAL 35-40 feet), medium boats (OAL 40-50 feet) and large boats (OAL up to 80 feet) operate from this harbour

Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters

Hypoxia exerts profound effects on cell physiology, but its effect on colonic uptake of the microbiota-generated forms of vitamin B1 (i.e., thiamin pyrophosphate [TPP] and free thiamine) has not been described. Here, we used human colonic epithelial NCM460 cells and human differentiated colonoid monolayers as in vitro and ex vivo models, respectively, and were subjected to either chamber (1%

PocketMatch: A new algorithm to compare binding sites in protein structures

Background: Recognizing similarities and deriving relationships among protein molecules is a fundamental
requirement in present-day biology. Similarities can be present at various levels which can be detected through comparison of protein sequences or their structural folds. In some cases similarities obscure at these levels could be present merely in the substructures at their binding sites. Inferring functional similarities between protein molecules by comparing their binding sites is still largely exploratory and not as yet a routine protocol. One of
the main reasons for this is the limitation in the choice of appropriate analytical tools that can compare binding sites with high sensitivity. To benefit from the enormous amount of structural data that is being rapidly accumulated, it is essential to have high throughput tools that enable large scale binding site comparison.

Results: Here we present a new algorithm PocketMatch for comparison of binding sites in a frame invariant
manner. Each binding site is represented by 90 lists of sorted distances capturing shape and chemical nature of the site. The sorted arrays are then aligned using an incremental alignment method and scored to obtain PMScores for pairs of sites. A comprehensive sensitivity analysis and an extensive validation of the algorithm have been carried out. Perturbation studies where the geometry of a given site was retained but the residue types were changed randomly, indicated that chance similarities were virtually non-existent. Our analysis also demonstrates that shape information alone is insufficient to discriminate between diverse binding sites, unless
combined with chemical nature of amino acids.

Conclusions: A new algorithm has been developed to compare binding sites in accurate, efficient and
high-throughput manner. Though the representation used is conceptually simplistic, we demonstrate that along
with the new alignment strategy used, it is sufficient to enable binding comparison with high sensitivity. Novel methodology has also been presented for validating the algorithm for accuracy and sensitivity with respect to geometry and chemical nature of the site. The method is also fast and takes about 1/250th second for one comparison on a single processor. A parallel version on BlueGene has also been implemented

Adoption of Sea Cage, Culture Practices in Thoothukudi District Tamil Nadu: An Eye Opener for Income Generation

Adoption of Sea Cage, Culture Practices in Thoothukudi District Tamil Nadu: An Eye Opener for Income Generatio

पिंजरा मछ्ली पालन पद्धति के इंजनीयरी और तकनीकी पहलू

कृपया पूरा लेखा पढ

Incidence of amyloodiniosis in snubnose pompano juveniles and its effective control in marine hatchery

Amyloodiniosis infection in snubnose pompano was reported in marine fish hatcheries of ICAR-CMFRI during preparation for certain feeding experiments in this fish that is of aquaculture importance. The control and elimination of Amyloodinium sp. from the hatchery system is a herculean but vital task and several methods were carried out following the infection.These were evaluated for their usefulness and are reported below

Account of bivalve fishery resources of Southern Tamil Nadu

Account of bivalve fishery resources of Southern Tamil Nad

Larviculture and seed production of the silver pompano, Trachinotus blochii (Lacepede, 1801) for the first time in India

Larviculture and seed production protocols for the silver pompano Trachinotus blochii, which is one of the promising finfish species for brackishwater and marine aquaculture was developed based on six trials for the first time in India. The newly hatched larvae were stocked at a density of 20 larvae l-1 in 2 t FRP tanks with 1.5 t of seawater. Green water technique was employed during larviculture upto the weaning period. Enriched S-type rotifers were fed at a density of 10-12 nos. ml-1 until 3 day post hatch (dph) and the enriched L-type rotifers were given at a density of 6-8 nos. ml-1 from 7 dph and thereafter with enriched Artemia at a density of 3-5 nos. ml-1. Weaning started from 15 dph and by 20-25 dph metamorphosis was completed. The survival on completion of metamorphosis ranged from 10-15% and the specific growth rate in terms of length (mm) was 8% per day. The larval morphology and growth pattern are also described