VI Workshop on Computational Data Analysis and Numerical Methods: Book of Abstracts

The VI Workshop on Computational Data Analysis and Numerical Methods (WCDANM) is going to be held on June 27-29, 2019, in the Department of Mathematics of the University of Beira Interior (UBI), Covilhã, Portugal and it is a unique opportunity to disseminate scientific research related to the areas of Mathematics in general, with particular relevance to the areas of Computational Data Analysis and Numerical Methods in theoretical and/or practical field, using new techniques, giving especial emphasis to applications in Medicine, Biology, Biotechnology, Engineering, Industry, Environmental Sciences, Finance, Insurance, Management and Administration. The meeting will provide a forum for discussion and debate of ideas with interest to the scientific community in general. With this meeting new scientific collaborations among colleagues, namely new collaborations in Masters and PhD projects are expected. The event is open to the entire scientific community (with or without communication/poster)

Radon concentration assessment in water sources of public drinking of Covilhã's county, Portugal

Radon, the heaviest of the noble gases on the periodic table of elements, is a natural radioactive element that can be found on water, soils and rocks. The main goal of this work is to present an evaluation of radon concentration on samples of water, used for human consumption, collected on uranium-rich granitic rock areas. Once the geological features of the sampling region evidence the presence of this natural radionuclides, their slow dissolution steadily increases concentration in ground water. Although, the most important contribution of natural radiation, for most populations, is from inhaled radon (generic term used commonly to refer to the isotope 222Rn), in some circumstances, exposure to natural radionuclides, through drinking water, could exceed acceptable levels, and also present a hazard. Despite the fact that radon can be reduced if the water is boiled, this gas, dissolved in ground water, can be released into the air during household activities such as showering, dishwashing and laundry. So, the short lived radon decay products will contribute to increase the number of those which are present in particles suspended in the indoor air and can be accumulated up to dangerous concentrations. Once the radon progeny emits highly ionizing alpha-radiation, they may cause substantial health damage after long-term exposure. Radon concentration measurements were performed on thirty three samples collected from water wells at different depths and types of aquifers, at Covilhã's County, Portugal with the radon gas analyser DURRIDGE RAD7. Twenty three, of the total of water samples collected, gave, values over 100 Bq/L, being that 1690 Bq/L was the highest measured value.info:eu-repo/semantics/publishedVersio

Punching of reinforced concrete flat slabs – Rational use of high strength concrete

PTDC/ECI-EST/30511/2017; SFRH/BD/76242/2011; The authors are grateful to CIMPOR for providing the cement and Sika for providing VISCOCRETE 20HE and SIKACRETE HD.This paper deals with punching of reinforced high strength concrete (HSC) flat slabs. Despite the use of HSC increased significantly in the last years, the experimental research on punching behavior of HSC slabs is still limited. Furthermore, most of this past research adopted concrete compressive strength lower than 90 MPa. In a previous work by this research group three specimens with concrete compressive strength around 120 MPa and one with normal strength concrete (NSC) were tested. The present work represents the continuation of the previous activity and it is focused on the rational use of HSC. Four specimens with HSC and one of NSC were tested under monotonic vertical loading. The HSC was placed only in the slab-column connection region and it was limited to a thin layer in the compressive zone, in order to have a more economical and sustainable solution. This rational use of the HSC showed excellent results in terms of punching strength. Limiting the HSC to a thin layer in the compressive zone resulted in an almost equal punching strength to that obtained with the slab entirely casted in HSC.authorsversionpublishe

Generation of a gene-corrected human induced pluripotent stem cell line derived from a patient with laterality defects and congenital heart anomalies with a c.455G > A alteration in DAND5.

This work was supported by Fundação para a Ciência e a Tecnologia (PTDC/ BIM-MED/3363/2014) and iNOVA4Health -UID/Multi/04462/ 2013, a program financially supported by Fundação para a Ciência e Tecnologia/ Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement.Human induced pluripotent stem cells (hiPSCs) from individual patient basis are considered a powerful resource to model human diseases. However, to study complex multigenic diseases such as Congenital Heart Disease, it is crucial to generate perfect isogenic controls to understand gene singularity and contribution. Here, we report the engendering of an isogenic hiPSC line with homozygous correction of c.455G > A alteration in the DAND5 gene, using CRISPR/Cas9 technology. The characterization of a clone of this cell line demonstrates normal karyotype, pluripotent state, and potential to differentiate in vitro towards endoderm, mesoderm, and ectoderm.publishersversionpublishe

Are CSR leaders less prone to engage in impression management?

This study examines the readability of corporate communication in the CEO letters included in the corporate social responsibility (CSR) reports presented by the firms that comprise the S&P 500 Index. These documents were content analyzed through the use of an automated algorithm provided by Readable.IO. Using a frame of analysis based on the social psychology theory of impression management, we studied the impression management tactics used. The main findings suggest that leading CSR companies (those listed in the Dow Jones Sustainability Index) present more readable CSR information in terms of comprehension and extension. These companies disclose CSR information generally in a positive way. However, these disclosure patterns are mediated by the “goal relevance of the impressions” and the “value of desired goals” related to the impression management tactics usedinfo:eu-repo/semantics/publishedVersio

Epigenetic and Drug Response Modulation of Epigalocaten-In-3-Gallate in Staphylococcus aureus with Divergent Resistance Phenotypes

Healthcare-associated methicillin-resistant Staphylococcus aureus infections represent extremely high morbidity and mortality rates worldwide. We aimed to assess the antimicrobial potential and synergistic effect between Epigalocatenin-3-gallate (EGCG) and different antibiotics in S. aureus strains with divergent resistance phenotypes. EGCG exposure effects in epigenetic and drug resistance key modulators were also evaluated. S. aureus strains (n = 32) were isolated from infected patients in a Lisbon hospital. The identification of the S. aureus resistance phenotype was performed through automatized methods. The antibiotic synergistic assay was performed through disk diffusion according to EUCAST guidelines with co-exposure to EGCG (250, 100, 50 and 25 µg/mL). The bacteria's molecular profile was assessed through FTIR spectroscopy. The transcriptional expression of OrfX, SpdC and WalKR was performed by using qRT-PCR. FTIR-spectroscopy analysis enabled the clear discrimination of MRSA/MSSA strains and the EGCG exposure effect in the bacteria's molecular profiles. Divergent resistant phenotypes were associated with divergent transcriptional expression of the epigenetic modulator OrfX, particularly in MRSA strains, as well as the key drug response modulators SpdC and WalKR. These results clearly demonstrate that EGCG exposure alters the expression patterns of key epigenetic and drug response genes with associated divergent-resistant profiles, which supports its potential for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms.info:eu-repo/semantics/publishedVersio

First report of Thielaviopsis paradoxa causing stem rot in Dracaena marginata in Brazil.

Comunicação

Optimization of raw ewes’ milk high-pressure pre-treatment for improved production of raw milk cheese

Serra da Estrela protected designation of origin (PDO) cheese is manufactured with raw milk from Bordaleira and/or Churra Mondegueira da Serra da Estrela sheep breeds. Several socioenvironmental shortcomings have reduced production capacity; hence, treatments that may contribute to its efficient transformation into cheese are welcome. High-pressure processing (HPP) milk pre-treatment may contribute to a cheese yield increment, yet optimization of processing conditions is warranted. An initial wide-scope screening experiment allowed for pinpointing pressure intensity, holding time under pressure and time after HPP as the most important factors influencing curd yield. Based on this, a more targeted screening experiment allowed for selecting the range of experimental conditions to be used for an experimental design study that revealed an HPP treatment at 121 MPa for 30 min as the optimum for milk processing to improve curd yield (>9%) and effectively maintain the beneficial cheese microbiota; the optimum was validated in a final experimental framework.info:eu-repo/semantics/publishedVersio