149 research outputs found
The Optimal Gravitational Lens Telescope
Given an observed gravitational lens mirage produced by a foreground
deflector (cf. galaxy, quasar, cluster,...), it is possible via numerical lens
inversion to retrieve the real source image, taking full advantage of the
magnifying power of the cosmic lens. This has been achieved in the past for
several remarkable gravitational lens systems. Instead, we propose here to
invert an observed multiply imaged source directly at the telescope using an
ad-hoc optical instrument which is described in the present paper. Compared to
the previous method, this should allow one to detect fainter source features as
well as to use such an optimal gravitational lens telescope to explore even
fainter objects located behind and near the lens. Laboratory and numerical
experiments illustrate this new approach
A diamond AGPM coronagraph for VISIR
In recent years, phase mask coronagraphy has become increasingly efficient in imaging the close environment of stars, enabling the search for exoplanets and circumstellar disks. Coronagraphs are ideally suited instruments, characterized by high dynamic range imaging capabilities, while preserving a small inner working angle. The AGPM (Annular Groove Phase Mask, Mawet et al. 2005) consists of a vector vortex induced by a rotationally symmetric subwavelength grating. This technique constitutes an almost unique solution to the achromatization at longer wavelengths (mid-infrared). For this reason, we have specially conceived a mid-infrared AGPM coronagraph for the forthcoming upgrade of VISIR, the mid-IR imager and spectrograph on the VLT at ESO (Paranal), in collaboration with members of the VISIR consortium. The implementation phase of the VISIR Upgrade Project is foreseen for May-August 2012, and the AGPM installed will cover the 11-13.2 ÎŒm spectral range. In this paper, we present the entire fabrication process of our AGPM imprinted on a diamond substrate. Diamond is an ideal material for mid-infrared wavelengths owing to its high transparency, small dispersion, extremely low thermal expansion and outstanding mechanical and chemical properties. The design process has been performed with an algorithm based on the rigorous coupled wave analysis (RCWA), and the micro-fabrication has been carried out using nano-imprint lithography and reactive ion etching. A precise grating profile metrology has also been conducted using cleaving techniques. Finally, we show the deposit of fiducials (i.e. centering marks) with Aerosol Jet Printing (AJP). We conclude with the ultimate coronagraph expected performances
SPICES: Spectro-Polarimetric Imaging and Characterization of Exoplanetary Systems
SPICES (Spectro-Polarimetric Imaging and Characterization of Exoplanetary
Systems) is a five-year M-class mission proposed to ESA Cosmic Vision. Its
purpose is to image and characterize long-period extrasolar planets and
circumstellar disks in the visible (450 - 900 nm) at a spectral resolution of
about 40 using both spectroscopy and polarimetry. By 2020/22, present and
near-term instruments will have found several tens of planets that SPICES will
be able to observe and study in detail. Equipped with a 1.5 m telescope, SPICES
can preferentially access exoplanets located at several AUs (0.5-10 AU) from
nearby stars (25 pc) with masses ranging from a few Jupiter masses to Super
Earths (2 Earth radii, 10 M) as well as circumstellar
disks as faint as a few times the zodiacal light in the Solar System
Exoplanet Characterization and the Search for Life
Over 300 extrasolar planets (exoplanets) have been detected orbiting nearby
stars. We now hope to conduct a census of all planets around nearby stars and
to characterize their atmospheres and surfaces with spectroscopy. Rocky planets
within their star's habitable zones have the highest priority, as these have
the potential to harbor life. Our science goal is to find and characterize all
nearby exoplanets; this requires that we measure the mass, orbit, and
spectroscopic signature of each one at visible and infrared wavelengths. The
techniques for doing this are at hand today. Within the decade we could answer
long-standing questions about the evolution and nature of other planetary
systems, and we could search for clues as to whether life exists elsewhere in
our galactic neighborhood.Comment: 7 pages, 2 figures, submitted to Astro2010 Decadal Revie
Regulation of early signaling and gene expression in the α-particle and bystander response of IMR-90 human fibroblasts
<p>Abstract</p> <p>Background</p> <p>The existence of a radiation bystander effect, in which non-irradiated cells respond to signals from irradiated cells, is well established. To understand early signaling and gene regulation in bystander cells, we used a bio-informatics approach, measuring global gene expression at 30 minutes and signaling pathways between 30 minutes and 4 hours after exposure to α-particles in IMR-90 fibroblasts.</p> <p>Methods</p> <p>We used whole human genome microarrays and real time quantitative PCR to measure and validate gene expression. Microarray analysis was done using BRB-Array Tools; pathway and ontology analyses were done using Ingenuity Pathway Analysis and PANTHER, respectively. We studied signaling in irradiated and bystander cells using immunoblotting and semi-quantitative image analysis.</p> <p>Results</p> <p>Gene ontology suggested signal transduction and transcriptional regulation responding 30 minutes after treatment affected cell structure, motility and adhesion, and interleukin synthesis. We measured time-dependent expression of genes controlled by the NF-ÎșB pathway; matrix metalloproteinases 1 and 3; <it/>chemokine ligands 2, 3 and 5 and <it/>interleukins 1ÎČ, 6 and 33. There was an increased response of this set of genes 30 minutes after treatment and another wave of induction at 4 hours. We investigated AKT-GSK3ÎČ signaling and found both AKT and GSK3ÎČ are hyper-phosphorylated 30 minutes after irradiation and this effect is maintained through 4 hours. In bystander cells, a similar response was seen with a delay of 30 minutes. We proposed a network model where the observed decrease in phosphorylation of ÎČ-catenin protein after GSK3ÎČ dependent inactivation can trigger target gene expression at later times after radiation exposure</p> <p>Conclusions</p> <p>These results are the first to show that the radiation induced bystander signal induces a widespread gene expression response at 30 minutes after treatment and these changes are accompanied by modification of signaling proteins in the PI3K-AKT-GSK3ÎČ pathway.</p
Technology for a Mid-IR Flagship Mission to Characterize Earth-like Exoplanets
The exploration of Earth-like exoplanets will be enabled at mid-infrared wavelengths
through technology and engineering advances in nulling interferometry and precision formation
flying. Nulling interferometry provides the dynamic range needed for the detection of
biomarkers. Formation flying provides the angular resolution required in the mid-infrared to
separately distinguish the spectra of planets in multi-planet systems. The flight performance
requirements for nulling have been met and must now be validated in a flight-like environment.
Formation-flying algorithms have been demonstrated in the lab and must now be validated in
space. Our proposed technology program is described
Transient Alteration of Cellular Redox Buffering before Irradiation Triggers Apoptosis in Head and Neck Carcinoma Stem and Non-Stem Cells
Background: Head and neck squamous cell carcinoma (HNSCC) is an aggressive and recurrent malignancy owing to intrinsic radioresistance and lack of induction of apoptosis. The major focus of this work was to design a transient glutathione depleting strategy during the course of irradiation of HNSCC in order to overcome their radioresistance associated with redox adaptation. Methodology/Principal Findings: Treatment of SQ20B cells with dimethylfumarate (DMF), a GSH-depleting agent, and L-Buthionine sulfoximine (BSO), an inhibitor of GSH biosynthesis 4 h before a 10 Gy irradiation led to the lowering of the endogenous GSH content to less than 10 % of that in control cells and to the triggering of radiation-induced apoptotic cell death. The sequence of biochemical events after GSH depletion and irradiation included ASK-1 followed by JNK activation which resulted in the triggering of the intrinsic apoptotic pathway through Bax translocation to mitochondria. Conclusions: This transient GSH depletion also triggered radiation-induced cell death in SQ20B stem cells, a key event to overcome locoregional recurrence of HNSCC. Finally, our in vivo data highlight the relevance for further clinical trials o
In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice
Brucella are facultative intracellular bacteria that chronically infect humans and animals causing brucellosis. Brucella are able to invade and replicate in a broad range of cell lines in vitro, however the cells supporting bacterial growth in vivo are largely unknown. In order to identify these, we used a Brucella melitensis strain stably expressing mCherry fluorescent protein to determine the phenotype of infected cells in spleen and liver, two major sites of B. melitensis growth in mice. In both tissues, the majority of primary infected cells expressed the F4/80 myeloid marker. The peak of infection correlated with granuloma development. These structures were mainly composed of CD11b+ F4/80+ MHC-II+ cells expressing iNOS/NOS2 enzyme. A fraction of these cells also expressed CD11c marker and appeared similar to inflammatory dendritic cells (DCs). Analysis of genetically deficient mice revealed that differentiation of iNOS+ inflammatory DC, granuloma formation and control of bacterial growth were deeply affected by the absence of MyD88, IL-12p35 and IFN-Îł molecules. During chronic phase of infection in susceptible mice, we identified a particular subset of DC expressing both CD11c and CD205, serving as a reservoir for the bacteria. Taken together, our results describe the cellular nature of immune effectors involved during Brucella infection and reveal a previously unappreciated role for DC subsets, both as effectors and reservoir cells, in the pathogenesis of brucellosis
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